Fitogênicos ou mono e diglicerídeos associados com ácidos orgânicos em dietas para leitões recém-desmamados

Pós-graduação em Zootecnia - FCAV

Probiótico e ácidos orgânicos na alimentação inicial de frangos de corte: Desempenho zootécnico, morfometria e microbiologia intestinal

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Efeitos da adição de agentes tróficos na dieta de leitões desmamados sobre a expressão da enzima ornitina descarboxilase, os conteúdos de proteína e DNA e o desempenho

Two experiments were carried out to evaluate the effect of adding glutamine, fish oil or yeast cellular wall to the diet of weaned piglets on the expression of the enzyme ornithine decarboxylase (ODC), the protein content and DNA in small intestine samples and on performance. In the first experiment, 24 weaned piglets were used to measure the performance at the phases prestarter, starter 1 and starter 2. Four diets were tested (T1 -basal diet (BD); T2 -BD + 1% of glutamine; T3 -BD + 0,2% of yeast cellular wall; T4 -BD + 5% of fish oil). At the second experiment 45 weaned piglets were used and distributed in a randomized block design, in factorial outline with four diets and three slaughter ages (on the day of weaning, on the seventh and fourteenth days postweaning). The tested diets did not alter the piglets' performance in none of the phases. There was reduction of the expression of the ODC enzyme, of the protein concentration and of the relationship protein/DNA to the seven days postweaning, with increase of the values on the 14th day, evidencing a state of hypotrophy of the mucous membrane, suggesting that the process of protein synthesis in the small intestine was diminished in the first week after weaning, but it presented recovery signs in the second week.

Outcome of pigs with short gut syndrome submitted to orthotopic intestinal transplantation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of microbiological quality of drinking water in the intestinal morphology of broilers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Intestinal integrity and performance of broiler chickens fed a probiotic, a prebiotic, or an organic acid

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Benefits of caloric restriction in the myenteric neuronal plasticity in aging rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Performance, carcass characteristics and non-carcass components of Texel × Santa Inês lambs fed fat sources and monensin

Performance, carcass, non-carcass and commercial cuts and components of Texel × Santa Inês crossbred lambs, managed in confinement and fed diets based on soybean oil, soybeans and a conventional diet, with or without the use of monensin (78 ppm dry matter basis) were evaluated. Thirty-six Texel × Santa Inês lambs (18 males and 18 females) were managed in intensive systems. Animals were slaughtered after 87 days of confinement, and performance, carcass characteristics, weight and percentages of carcass and non-carcass components were evaluated. The animals fed the control diet had heavier carcass and parts than animals fed soybean, while the oil diet did not differ from the controls in most parameters. The animals fed soybean showed lower intake kg dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and metabolizable energy (ME) compared with animals fed the control diet, increased ether extract (EE) intake in kg, % body weight (BW) and metabolic weight (MW) and did not differ from the soybean oil diet. Animals receiving monensin had lower DM intake, OM, CP, EE, NDF, gross energy (GE) regardless of the expression, % kg BW, or % PM, than the animals that did not receive the additive. Males produced better and had heavier cuts than the females; the latter deposited subcutaneous fat earlier. Animals that received oil or soybean showed greater body weight and small intestine percentage. Soybean oil intake did not improve performance, carcass weights or parts of Santa Ines × Texel lambs in confinement. Soybeans at 15% dry matter reduced energy intake and lamb performance. The use of monensin at 78 ppm on a dry matter basis is not recommended for lambs in confinement, especially if associated with oil or soybeans that may harm animal performance.

Determinação sérica de haptoglobina, ceruloplasmina, alpha(1)-glicoproteína ácida, transferrina e alpha(1)-antitripsina, em equinos com cólica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization of a monoclonal antibody recognizing mast cells

Immunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells.

Mananas e glucanas em dietas para leitões recém-desmamados

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Histomorphometric study of role of lactoferrin in atrophy of the intestinal mucosa of rats

The purpose of this work was to study the effects of oral administration of lactoferrin (Lf) in rats subjected to atrophy of the small intestine induced by a diet based on soy protein concentrate as the main protein source. We used 24 male Wistar rats aged 40 days, kept in individual cages under appropriate conditions of temperature, light and humidity. The animals were divided into four groups (n = 6); 1) group SL received soy-based food and, once a day, a supplement of 200mg/kg of Lf administered by gavage; 2) group Si received the soy feed without supplement of Lf; 3) group CL received a diet based on casein plus Lf; 4) group Ci received the casein diet without supplement of Lf. At the end of fifteen days, a 10 mm segment of the initial portion of the small intestine was sectioned and subjected to morphometry of the intestinal crypts and villi and assessment of the number and size of myofibroblasts. Comparison between groups showed that the length of the villi was similar in groups Ci and CL and higher in CL than in SL; SL than in Si, in Ci than in SL, and in Ci than in Si to Ci. The crypt depth was similar in SL and CL, SL and Ci and Ci and CL and was higher in Si than in Ci and in Si than in SL. The number of myofibroblasts was higher in SL than in CL, in SL than in Si, in CL than in Ci, and in SL than in and Ci; between Ci and Si there was no difference. The area of myofibroblasts was similar between the groups SL and CL and Si and Ci and higher in SL than in Si, and in Cl than in and Ci, and in SL than in Ci. All statistical analysis assumed significance when p < 0.05. From these results, we conclude that lactoferrin increases the number and size of the pericrypt myofibroblasts and stimulates rapidly the regeneration of atrophied villi.

Ácidos orgânicos em dietas de suínos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aditivo alternativo, associado ou não ao antimicrobiano, na dieta de leitões recém-desmamados

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Análise da eficiência da biomassa de banana verde como complemento alimentar na melhora da constipação em pacientes funcionais e associada ao pós-operatório da Doença de Hirschdprung

Pós-graduação em Bases Gerais da Cirurgia - FMB