Ação do diclofenaco de sódio, após a interrupção da peritonite estercorácea, no cólon de ratos. Estudo da resistência mecânica e do colágeno tecidual.

The objective of the present study was to investigate changes in colon wall in rats with fecal peritonitis (Per) associated with sodium diclofenac (SD) by studying breaking strength and tissue collagen concentration. The rats were divided into the following experimental groups: GROUP 1-SD: 60 animals injected intramuscularly with sodium diclofenac at the dose of 2 mg/kg body weight; GROUP 2-Per: 60 animals injected intraperitoneally with a suspension of human feces. Peritonitis was interrupted after six hours of evolution; GROUP 3-Per+SD: 60 animals injected intraperitoneally with a suspension of human feces and receiving SD according to the schedule used for Groups 1 and 2; CONTROL GROUP: 12 animals injected intramuscularly with physiological saline. The animals of Group 1, 2 and 3 were successively sacrificed 2, 4, 7, 14 and 21 days after interruption of peritonitis an/or the beginning of treatment. Under conditions of the experimental model and of the methods used, we conclude that sodium diclofenac, peritonitis and the peritonitis-sodium diclofenac association decrease the breaking strength and the concentration of tissue collagen in the colon segment.

Estudo sobre a resistência aos antimicrobianos em bactérias isoladas de pacientes hospitalizados (Botucatu, 1988-1992)

Since 1988 to 1992, a study about susceptibility to antimicrobial drugs of bacterias isolated from hospitalized patients was performed. The compared susceptibility to important drugs (ampicilin, cephalothin, cefoxitin, ceftazidime, ceftriaxone, aztreonam, gentamicin, amikacin, peftoxacin, ciprofloxacin, imipenem, oxacillin and vancomicin) was investigated in 1200 strains (300 of each specie) of the prevalent bacterias: E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and S. aureus. Minimal inhibitory concentration (MIC) was determined by agar dilution method, using from 0,05 to 256 mcg of each drug per ml of culture medium (Mueller-Hinton). Ranges of MIC, MIC 50%, MIC 90% and the proportion of resistant strains were determined and permited to know the 4 drugs that were found to be more active against bacterias; the CIM 90% values are: E. coli - aztreonam (0,1 mcg/ml), pefloxacin (0,1), ceftazidime (0,25) and ceftriaxone (0,05); K. pneumoniae - aztreonam (0,25), ceftriaxone (0,25), ceftazidime (0,5) and pefloxacin (2,0); P. aeruginosa - imipenem (4,0), aztreonam (16), ceftazidime (16) and ciprofloxacin (16); S. aureus - vancomicina (1,0), ciprofloxacin (8,0), arnicacina (128) and cephalothin (128 mg/ml). The better in vitro antibacterial activity observed was related to: aztreonam (77-100% of the sensitive strains), ceftazidime (50-99,7%), pefloxacin (73-99,7%), ciprofloxacin(80%), imipenem (93%) and vancomicin (100%).

Estimativa da mudança genética na produção de leite e gordura em bovinos da raça Holandesa

The objective of this study was to estimate the genetic trends of the milk and fat yield in three herds maintained in São Paulo State, Brazil. The estimation of genetic, environmental and phenotypic trends were based on 716 first lactations. The cows were sired by 134 bulls. The statistical models included the fixed effects of herds, kind of gestation, season and year of the calving, age of the cow at calving, besides the random effect of the bulls. The statistical analyses, by the last squares method showed effects for bulls, herds and age of the cows on milk and fat yield. The genetic, environmental and phenotypic trends estimated were -10.20; 6.74 and -3.46 kg for milk yield and -1.90; 2.20 and 0.12 kg for fat yield.

Tipos de resistência a Alabama Argillacea (Huebner, 1818) (Lepidoptera: Noctuidae) envolvidos em genótipos de algodoeiro: II. Antibiose

Cotton genotypes resistance to Alabama argillacea (Huebner, 1818) due to antibiosis has been evaluated in laboratory trials (at 27 ± 2°C, 70 ± 10% relative humidity and 14 hours photoperiod). Six different genetic materials (T 1122-13-1, STO 285 N, JPM 157, T 953-13-4-2, CNPA 9211-21, and CNPA 9211-31) have been tested in a completely randomized experimental design, with ten replications, each one with ten larvae/genotype. Larvae have been supplied with leaves daily. The following variables have been scored: time length of several insect stages (from larvae to adult), average mass of larvae and pupae as well as average larval, pre-pupal and pupal mortality rates. All genotypes have revealed longer overall average time length of stage duration from larvae to adult, as compared to STO 285 N; however, CNPA 9211-31 and CNPA 9211-21 have shown the smallest average mass of pupae along with high frequencies of pre-pupae and larvae to adult mortalities. The results strongly suggest that antibiosis is one of the resistance mechanisms to A. argillacea in that two cotton genotypes.

Resistência ao Cisalhamento de um Latossolo sob Diferentes uso e Manejo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diagnóstico de uma área compactada por atividade minerária, na floresta amazônica, empregando métodos geoestatísticos à variável resistência mecânica à penetração do solo

A quite common problem in the recovery of degraded areas in the mineral exploration understands the compaction of the soil due to the intense traffic of machines and earth movement. The most common problem of the compaction of a degraded surface is: increase of the mechanical resistance to the penetration of the rooats, reduction of the aeration, alteration of the water flow and heat. Thus the present work had the basic objective of diagnosing the compaction of a degraded area by mining in a space way, through the mechanical resistance the way penetration to guide a future subsoiling in the place seeking recovery. Through the studies it was concluded that the kriging method in agreement with the space variation allows the division of the area studies in sub areas facilitating a future work to reduce cost and unnecessary interference to the atmosphere. The method was shown quite appropriate and it can be used in diagnosis of the compaction in a degraded area by mining, foreseeing subsoiling need.

Resistência à penetração de um solo em recuperação sob sistemas agrosilvopastoris

In the Research and Teaching Farm of the State University of São Paulo (UNESP) - Campus of Ilha Solteira, located in Selviria, in Mato Grosso do Sul State, a study was conducted with the objective of investigating the resistance to penetration of a highly degraded soil due to construction of a Hydroelectric Power Plant. The experimental design was a completely randomized, with five treatments and five replications. The treatments were constituted of the following uses and managements: area in advanced state of degradation; area under regeneration cultivated 13 years with Pinus; area under regeneration cultivated 11 years with green manure and pasture; degraded area with natural regeneration; and area with natural vegetation (savanna). The system where the area was under regeneration with Pinus cultivation and the niches with natural regeneration were the closest to natural condition (savanna). Except for the natural area (savanna), all others presented a higher resistance to penetration in the layer of 0.20-0.40 m, which represents serious limitations for growth of plant roots.

Correlação da produtividade do feijão com a resistência à penetração do solo sob plantio direto

The soil mechanical resistance to penetration (PR) has great influence on vegetative development as the root growth and the crop productivity change in inverse proportion. Thus, the objective of this research was to study correlation between the bean grain productivity and the PR in an Oxisol cultivated for four years in no-tillage system at FEIS/UNESP. The attributes PR and yield were determined in a regular grid with 119 sample points. The PR was determined in the layers of 0-0.05, 0.05-0.10, 0.10-0.15, 0.15-0.20 and 0.25-0.30 m. The results were submitted to procedures of descriptive statistics, linear correlation and geostatistic analysis. The linear correlation between the yield and PR was practically null, as in all soil layers investigated it showed determination coefficients (R2) smaller than 0.03 and not significant. The geostatistic analysis showed moderate structure of spatial dependency for PR in the layers of 0.05-0.10 and 0.10-0.15 m, and strong for yield; however, the conjugate spatial analysis of such attributes showed no correlation, therefore, the spatial variability of PR did not influence the yield.

Condutividade hidráulica e resistência à penetraçao do solo influenciada por diferentes sistemas de manejo

Soil management measures that increase the efficiency of organic matter cycling and maintain favorable soil structure are needed for improving soil quality. On the other hand, soil structure degradation due to inadequate soil management systems is widespread. Among the indicators of soil physical quality, saturated hydraulic conductivity and penetration resistance are thought to be sensitive to soil management system. The aim of this work was to study the influence of soil tillage system and organic fertilization on selected soil physical properties after the first year of treatment. The field work was conducted in Selviria, MS, Brazil on an Oxisol. The experimental design was randomized complete blocks with split-plots, with 12 treatments and 4 repetitions. Tillage treatments included conventional ploughing (CT) and direct drilling (DD). Fertilizer treatments were: 1) manure, 2) manure plus mineral, 3) traditional mineral 4) plant residues of Crotalaria juncea, 5) plant residues of Pennisetum americanum and 6) control plot. The plots were cropped to bean in winter and to cotton in summer, and both cultures were irrigated. After one year no significant differences between treatments in mechanical resistance and porosity were found. However, saturated hydraulic conductivity and infiltration were higher in the conventional tillage treatment at the 0.00-0.10 m depth. Moreover, an improvement in soil physical condition by organic fertilizers was shown.

Resistência à compressão do concreto autoadensável: Influência da atividade pozolânica do calcário e do basalto

This research studies the influence of the pozzolanic activity of the calcareous and basalt in the resistence behavior of the compressive strength of high performance self-compacting concrete (HPSCC). The selected aditives are the calcareous filler and basalt filler, for they are industrial residues helping that way the sustainable development. The paste of this concrete type is constituted of cement, silica fume, calcareous filler or basalt filler, water and superplasticizer additive. In this research the relationships water/cement are fixed in 0,40 kg/kg, silica fume/cement of 0,10 kg/kg and the relationships filler/cement and superplasticizer/cement are determined through of Marsh́s cone and mini-slump tests. The granular skeleton is gotten from a composition between quartzous sand and brita of basalt that presents the lesser index of emptinesses. The results show that the HPSCC with the addition of calcareous filler has greater compressive strength than what the HPSCC with addition of basalt filler in the ages of 7, 28 and 63 days. It is explained by the fact that the calcareous filler presents greater index of pozzolanic activity than the basalt filler. Besides that the relation water/fine for the HPSCC with calcareous filler is 0,27 l/kg whereas the HPSCC with basalt filler is of 0,29 l/kg.

Efeito de um mês de destreinamento sobre a aptidão física relacionada à saúde em programa de mudança de estilo de vida

Physical exercise induces hemodynamic/ventilatory and neuromuscular adaptations that can be reverted with interruption of the training program. The aim of the present study was to evaluate the effect of detraining on physical fitness related to health. Forty-four healthy subjects, both male and female, aged 57.6±8.9 years performed the 'Mexa-se Pró-Saúde' protocol with nutritional orientation and supervised physical exercises for nine months. The program consisted of aerobic, localized muscular endurance and flexibility exercises, with duration 80 minutes/session, five days/week. Only subjects who participated in the program for more than three days/week have been selected. The detraining period was one month. Weight (kg) and height (m) were measured and the body mass index (BMI) calculated. Additionally, motors tests to evaluate the flexibility (FLEX), strength of lower limbs (SLL) and upper limbs (SUL), and maximal oxygen uptake (VO2máx) were conducted in the beginning of the study (MI), after nine months of practicing (MT) and after detraining period (MD). ANOVA (p<0.05) and Tukey test to show the difference between groups when it evidence were used for statistical treatment. The results showed that the gains of 22% and 7% on SLL and VO2máx respectively, obtained with the training, have not changed during the detraining period; however, the flexibility gain of 8% returned back to baseline after the detraining period. BMI and SUL did not change during the study. Although the lower limbs strength gains and maximal oxygen uptake obtained have been kept, one month of detraining was enough for losing the flexibility acquired.

Níveis constitutivos de compostos fenólicos podem estar relacionados à resistência da cana-de-açúcar à cigarrinha-das-raízes

The interaction of the leafhopper-of-roots with the sugar cane is still poorly marked and there is little information on resistant varieties. The phenolic compounds are involved in resistance mechanisms of the antibiosis type, but few studies are devoted to the studying role of these compounds in the interaction of plants with sucking insects. The study was conducted to determine how the sugar cane responded to the infestation of Mahanarva fimbriolata in terms of accumulation of phenolic compounds. An experiment was carried out under controlled conditions in a randomized design in a factorial schedule 3 x 2 x 4, with 3 genotypes and sugar cane, 2 levels of nymphs infestation of M. fimbriolata and sampling 4 times, with 4 repetitions. The genotypes SP80-1816 and RB72454 showed higher levels of total phenols when subjected to an infestation of the pest, but the duration of the nymphal stage and mortality of sharpshooters were significantly lower in those varieties, indicating that the increase in the concentration of phenolic compounds may had been caused by death and decay of the roots. It was not observed variation in levels of total phenols in the variety SP83- 5073 subject to an infestation of M. fimbriolata. However, the mortality of the leafhopperof- roots and duration of the stage of nymphs were significantly higher in genotype, indicating the existence of resistance-type antibiosis. Analysis of correlation showed that higher initial levels of phenolic compounds resulting in mortality of the pest can increase the duration of the nymph stage.

Determinação da resistência de Staphylococcus aureus: Um desafio?

The aim of this study was to identify the resistance profile of Staphylococcus aureus strains, in relation to induced clindamycin resistance, and to detect oxacillin resistance by the routine phenotypic methods. The strains were isolated from nasal or lingual swabs taken from healthy adult carriers with no medical history of hospitalization or antibiotic treatment. Eighteen strains were distinguished by the different patterns generated by pulsed field gel electrophoresis (PFGE). Four (22.2%) of these showed sensitivity to clindamycin by the conventional antibacterial susceptibility test, but demonstrated inducible resistance to it by the D-test. One strain (5.6%) was characterized as borderline oxacillin-resistant S. aureus (BORSA), and another (5.6%) as CA MRSA (community-associated methicillin-resistant Staphylococcus aureus). Both of these strains were shown to be cefoxitin susceptible by the disk diffusion test. The polymerase chain reaction (PCR) failed to detect the mecA gene in this last strain and it was thus classified as BORSA. These results show the importance of incorporating the D-test into the routine lab tests for S. aureus inducible clindamycin resistance and also of including the cefoxitin resistance test among the phenotypic methods for MRSA characterization.

Influência da contaminação da dentina por cimentos temporários na resistência da união de sistemas adesivos

Objective: The aim of this study was to assess the bond strength of adhesive systems to dentin contaminated by temporary cements with or without eugenol. Method: Flat dentin surfaces were obtained from twenty-four human third molars. With exception of the control group (n=8), the surfaces were covered with Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA) or Cavit (3M ESPE, St. Paul, MN, USA) and kept in an oven at 37°C for seven days. After removing the cements, the adhesive systems Adper Single Bond (3M ESPE, St. Paul, MN, USA) or Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japan) were applied in accordance with the manufacturers' recommendations, and then the crowns were constructed in of resin composite. The teeth were sectioned into specimens with a cross-sectional bond area of 0.81mm2, which were submitted to microtensile testing in a mechanical test machine at an actuator speed of 0.5mm/min. The data were analyzed by t- and ANOVA tests, complemented by Tukey tests (α=0.05). Results: For Adper Single Bond (3M ESPE, St. Paul, MN, USA), bond strength did not differ statistically (p>0.05) for all the experimental conditions. For Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japan), only the Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA) Group showed significantly lower bond strength (30.1 ± 13.8 MPa) in comparison with the other groups; control (38.9 ± 13.5 MPa) and Cavit (3M ESPE, St. Paul, MN, USA) (42.1 ± 11.0 MPa), which showed no significant difference between them. Conclusion: It was concluded that the previous covering of dentin with temporary cement containing eugenol had a deleterious effect on the adhesive performance of the self-etching system only.

Avaliação de Diferentes Compósitos na Resistência à Fratura de Núcleos de Preenchimento

Objective: To evaluate and compare the fracture strength of different composite resins used for core buildup. Method: Thirty-six bovine teeth were decoronated at the cervical third to standardize the length of specimens at 20 mm. Under constant irrigation, the canals were prepared with #5 Largo drills corresponding to the size and diameter of #3 Reforpost fiberglass post. The posts were cemented with Enforce resin sealer, being 16 mm inside the root canal and 4 mm outside the root canal, and the material was light-activated for 30 seconds at each side. The specimens were divided into 3 groups (n=12), in which cores (4 mm diameter and 5 mm high) were prepared from a prefabricated standard with three types of composite resins: Group 1: Z100 (3M), Group 2: Z250 (3M) and Group 3: P60 (3M). The specimens were fixed in a cylindrical device with an adaptor at 45o inclination. This device was adapted to a universal testing machine (EMIC) to simulate the force until fracture of the specimen. Data were subjected to ANOVA (p<0.05). Results: The Z250 resin cores presented the highest mean fracture strength (45.453 kgf), while the mean fracture strengths in Group 1 and Group 3 were 38.014 and 39.506 kgf, respectively. P60 caused the largest number of root fractures. Conclusion: Considering the characteristics and properties of the tested resins, Z250 appears as the most indicated for core buildup.