154 resultados para Plasmid incompatibility
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Pós-graduação em Medicina Veterinária - FMVZ
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Medicina Veterinária - FMVZ
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Pós-graduação em Química - IBILCE
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Pós-graduação em Odontologia - FOAR
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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A study of the subject indexing process in university libraries is presented, using a socio-cognitive approach to analyze the procedures, difficulties and perceptions of the librarians, users, and managers that take part in the process. Individual and group verbal protocols were applied in the real environment of the cataloguers, the university libraries. The results were the absence of systematic procedures for book subject analysis and representation, the incompatibility of the indexing language, and problems in subject retrieval from the catalog. It is concluded that there is a tendency in catalogs to act as databases. Therefore, the cataloguer should present a level of commitment in his task similar to that of an indexer who works in the production of bibliographic databases.
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We evaluated study of the indexing process in the cataloging at university libraries, using a socio-cognitive approach to analyze procedures, difficulties and perceptions of librarians, users, and managers. The methodology consisted of the Individual and Group Verbal Protocols applied in university libraries. The results were the absence of procedures for book subject analysis and representation, incompatibility of indexing language, and problems of subject retrieval from the catalog. We have concluded that is tendency of catalogs to act as databases. Therefore, the cataloguer should present a posture of commitment similar to that of an indexer who works in the production of these bases.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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A metilação de ilhas CpG em regiões regulatórias de vários genes tem sido descrita como um processo importante no silenciamento de genes supressores de tumor e diretamente envolvida no processo de carcinogênese de uma série de tumores. O estudo desses genes afetados pela metilação em tumores visa à procura de marcadores moleculares para o diagnóstico, prognóstico e tratamento de tumores, e também a caracterização do seu papel no processo biológico do câncer. O nosso grupo de pesquisa participou de um Projeto Temático que visa a identificação de genes metilados em tumores de cabeça e pescoço (processo 03/09497-3) e foi então proposta a utilização do sistema de duplo-híbrido de levedura como ferramenta no início da análise funcional destes genes. Dessa maneira, utilizando como isca o gene CRABP2 identificado como diferencialmente metilado em câncer de cabeça e pescoço, foi realizado o rastreamento de duplo-híbrido para a identificação de interações físicas proteína-proteína. Foram rastreados aproximadamente 2,1x105 transformantes neste sistema, dos quais 550 foram inicialmente positivos para His+. Desses, 182 transformantes confirmaram a marca His+ e foram testados para -galactosidase. Em seguida, 19 foram selecionados para passar pela etapa do “plasmid linkage”. Após esse teste, 9 clones confirmaram a ligação dos marcadores His+ e β-gal+ com a presença do plasmídeo LEU2 . Assim, após o sequenciamento dos insertos contidos nos clones identificados, ciclina D3 (CCND3), alfa-macroglobulina 2 (A2M) (2 clones), canal aniônico dependente de voltagem 2 (VDAC2), tubulina alfa 1 (TUBA1), tubulina alfa 2 (TUBA2), tubulina beta (TUBB), fator de ligação ao “enhancer” do gene interleucina 2 (ILF2) e desoxi-hipusina sintase (DHPS) emergiram como ligantes de CRABP2
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Equine neonatal isoerythrolysis is a neonatal foals’ illness. Results from the incompatibility of blood type between the foal and the mare and mediated by maternal antibody absorbed by the colostrum against foal’s red blood cells. Characterized by a type ll hypersensitivity reaction, where the exhibition of the organism to a strange antigen, that it takes the sensitization of the lymphocytes B that after the removal of the antigens by the reticule-endothelial system the production of immunoglobulin is decreased, with the formation of cellular immunological will cause the occurrence of the illness in foal of sensitized mares. The most important clinical signs are severe anemia and jaundice, and this illness should be differentiated of other as: hemolysis induced by bacterial toxins, diseases of the hepatobiliary system, disseminated intravascular coagulation and incompatibility in blood transfusions. Like the sensitization happens during the previous incompatible foal’s birth, most cases occur in foals of multiparous mares. However during the first pregnancy the mare can generate a foal with neonatal 7 isoerythrolysis if she have developed placental anomaly in the beginning of the pregnancy which blood cells in her circulation
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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This study aims to assess the evolution of the environmental debate, starting by the contextualization of its first appearance, and perform a theoretical and methodological analysis of its ramifications, especially regarding the two main lines of thought in economics: environmental economics and ecological economics. Once confirmed the incompatibility of economic growth per se with the environmental limits, the degrowth proposal will be presented, its theoretical basis and especially the means by which its creators aim to promote a economic, politic and social restructuring. Finally, the challenges regarding the degrowth implementation on both the developed Northern economies and the third-world or emerging Southern countries will be exposed
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV