Determination of disease biomarkers in Eucalyptus by comprehensive two-dimensional gas chromatography and multivariate data analysis

In this paper is reported the use of the chromatographic profiles of volatiles to determine disease markers in plants - in this case, leaves of Eucalyptus globulus contaminated by the necrotroph fungus Teratosphaeria nubilosa. The volatile fraction was isolated by headspace solid phase microextraction (HS-SPME) and analyzed by comprehensive two-dimensional gas chromatography-fast quadrupole mass spectrometry (GC. ×. GC-qMS). For the correlation between the metabolic profile described by the chromatograms and the presence of the infection, unfolded-partial least squares discriminant analysis (U-PLS-DA) with orthogonal signal correction (OSC) were employed. The proposed method was checked to be independent of factors such as the age of the harvested plants. The manipulation of the mathematical model obtained also resulted in graphic representations similar to real chromatograms, which allowed the tentative identification of more than 40 compounds potentially useful as disease biomarkers for this plant/pathogen pair. The proposed methodology can be considered as highly reliable, since the diagnosis is based on the whole chromatographic profile rather than in the detection of a single analyte. © 2013 Elsevier B.V..

Fertilisation residues alter leaf scleromorphy in an evergreen savannah shrub (Maprounea brasiliensis, Euphorbiaceae)

In the present study, we examined how residues of nitrogen (N), phosphorus (P) and calcium (Ca) fertilisers affect leaf anatomical traits in Maprounea brasiliensis (Euphorbiaceae), a typical and dominant cerrado (Brazilian savannah) species adapted to dystrophic soils. We predicted that fertiliser residues would alter qualitative and quantitative aspects of M. brasiliensis leaves and would decrease their scleromorphy. Leaves were sampled from plants that were growing in soils previously fertilised with N, P and Ca and in plants that were growing in soils without fertiliser residues. We measured the thickness of the cuticle, the epidermis of adaxial and abaxial surfaces, thickness of palisade parenchyma and spongy parenchyma, total thickness of the leaf, total area of the midrib and leaf mass per area (LMA). We found that plants under fertiliser residues produced fewer scleromorphic leaves with low LMA, thinner cuticle and epidermis and thicker palisade and spongy parenchyma. They also showed a decrease in the size and area occupied by the leaf midvein. However, plants under fertiliser residues produced similar leaf thickness as did the plants in the control group. Our results showed that residual effects of fertilisation changed structural patterns of a typical species of cerrado. Thus, further studies about fertilisation effects on leaf traits are needed because larger areas of the central cerrado are being occupied for agricultural production. © 2013 CSIRO.

New species of Tenuipalpus (Acari: Tenuipalpidae) from Semidecidual Forest remnants in the State of São Paulo, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Micropropagação e conservação in vitro de Croton antisyphiliticus Mart

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Potencial de atividade antimicrobiana in vitro de extratos vegetais do cerrado frente estirpes de Staphylococcus aureus

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito da sazonalidade no teor proteico e composição de aminoácidos no pólen apícola produzido em Botucatu, Estado de São Paulo

Pós-graduação em Zootecnia - FMVZ

Enxertia e caracterização de rambutanzeiro por aspectos foliares

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Avaliação do TaV (Thyrinteina arnobia vírus) no controle de Thyrinteina arnobia (Lepidoptera: geometridae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Nitrogênio em cobertura e bioestimulante aplicado via foliar em feijoeiro de inverno no sistema plantio direto

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pulverização conjugada à fertilização para controle de Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) em diferentes híbridos de milho, em função do volume de calda e ponta de pulverização

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Fertirrigação na produção e qualidade de mudas de Eucalyptus spp. nos períodos de inverno e de verão

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estoque de carborno em solos sob plantios de eucalipto e fragmento em Cerrado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Doses de N-Ureia e de esterco bovino na qualidade nutricional da alface em consórcio com a rúcula

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Fatores ambientais e fenológicos na atividade antimicrobiana e produção de ativos em Cochlospermum regium (Schrank) Pilg

Pós-graduação em Agronomia (Horticultura) - FCA

Identificação de raças de Bremia lactucae no Estado de São Paulo e desenvolvimento de linhagens de alface-crespa resistentes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)