An alternate impression technique for ocular prostheses

This article describes an alternative two-step ocular prosthesis impression technique that employs two materials of different consistencies. The method is intended to provide better adaptation to underlying tissues, increased mobility of the prosthesis owing to improvements in facial contours, and improved esthetics, as well as offering the patient greater comfort and security. These advantages and this prosthesis' relative ease of fabrication mean it should be considered as the first step in the management of untreated anophthalmic sockets. © 2012 by the American College of Prosthodontists.

Determining the gestational age of crioulo mares based on a fetal ocular measure

The practice of covering in herds is often used in Crioulo breeding farms, thus not allowing the determination of ovulation dates and the early detection of pregnancies. This fact complicates the estimation of gestational age after 70 days of conception. This study aimed to evaluate the diameter of the fetal orbits of Crioulo mares to develop a formula to estimate a gestational date. Measurements of 164 fetal ocular orbits were performed in 124 Crioulo mares with gestational ages ranging between 119 and 341 days. The current study observed a linear growth pattern of Crioulo fetal orbits up to 341 days of gestation. Thus, obtaining a suitable linear regression model to determine the gestational age of Crioulo mares based on fetal ocular orbit diameter measure is possible. The obtained model was y = 8.3756x + 11.90, where y represents the gestational age in days, and x represents the diameter of the fetal ocular orbit in millimeters. This formula had anr2 of 0.985 (P < .001). We conclude that this model allows researchers and veterinarians to accurately estimate the gestational age of Crioulo mares between 4 and 11months of pregnancy. © 2013 Elsevier Inc.

Persistent inflammation in the CNS during chronic EAE despite local absence of IL-17 production

Experimental autoimmune encephalomyelitis (EAE) is an artificially induced demyelination of the central nervous system (CNS) that resembles multiple sclerosis in its clinical, histopathological, and immunological features. Activated Th1 and Th17 cells are thought to be the main immunological players during EAE development. This study was designed to evaluate peripheral and local contribution of IL-17 to acute and chronic EAE stages. C57BL/6 mice were immunized with MOG plus complete Freund's adjuvant followed by pertussis toxin. Mice presented an initial acute phase characterized by accentuated weight loss and high clinical score, followed by a partial recovery when the animals reached normal body weight and smaller clinical scores. Spleen cells stimulated with MOG produced significantly higher levels of IFN-γ during the acute period whereas similar IL-17 levels were produced during both disease stages. CNS-infiltrating cells stimulated with MOG produced similar amounts of IFN-γ but, IL-17 was produced only at the acute phase of EAE. The percentage of Foxp3+ Treg cells, at the spleen and CNS, was elevated during both phases. The degree of inflammation was similar at both disease stages. Partial clinical recovery observed during chronic EAE was associated with no IL-17 production and presence of Foxp3+ Treg cells in the CNS. © 2013 Sofia Fernanda Gonçalves Zorzella-Pezavento et al.

SOCS3 expression correlates with severity of inflammation, expression of proinflammatory cytokines, and activation of STAT3 and p38 MAPK in LPS-induced inflammation in vivo

SOCS3 is an inducible endogenous negative regulator of JAK/STAT pathway, which is relevant in inflammatory conditions. We used a model of LPS-induced periodontal disease in rats to correlate SOCS3 expression with the inflammatory status. In vitro we used a murine macrophage cell line to assess the physical interaction between SOCS3 and STAT3 by coimmunoprecipitation. 30 ug of LPS from Escherichia coli were injected in the gingival tissues on the palatal aspect of first molars of the animals 3x/week for up to 4 weeks. Control animals were injected with the vehicle (PBS). The rats were sacrificed at 7, 15, and 30 days. Inflammation and gene expression were assessed by stereometric analysis, immunohistochemistry, RT-qPCR, and western blot. LPS injections increased inflammation, paralleled by an upregulation of SOCS3, of the proinflammatory cytokines IL-1β, IL-6, and TNF-and increased phosphorylation of STAT3 and p38 MAPK. SOCS3 expression accompanied the severity of inflammation and the expression of proinflammatory cytokines, as well as the activation status of STAT3 and p38 MAPK. LPS stimulation in a macrophage cell line in vitro induced transient STAT3 activation, which was inversely correlated with a dynamic physical interaction with SOCS3, suggesting that this may be a mechanism for SOCS3 regulatory function. © 2013 João Antônio Chaves de Souza et al.

Pico e flutuação da pressão intra-ocular: comparação entre curva tensional diária e teste de sobrecarga hídrica e comparação entre 2 testes de sobrecarga hídrica em horários diferentes

Pós-graduação em Bases Gerais da Cirurgia - FMB

Pressão intra-ocular, condições endoteliais e prostaglandina E2 no humor aquoso de cães com catarata senil madura ou hipermatura

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeitos da instilação de morfina na inflamação ocular induzida experimentalmente em coelhos

Pós-graduação em Cirurgia Veterinária - FCAV

Aspectos ultrassonográficos, biometria e dopplerfluxometria ocular de primatas-Alouatta fusca (BUGIO RUIVO-Geoffroy Saint-Hilaire, 1812)

Pós-graduação em Medicina Veterinária - FMVZ

Efeitos do maleato de timolol, do levobunolol e da apraclonidina sobre a pressão intra-ocular, o diâmetro pupilar, a frequência cardíaca e a pressão sanguínea em cães da raça Beagle

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação clinica e histopatológica da cicatrização de úlceras de córnea experimentais em coelhos, com deficiência de células límbicas, recobertas com membrana amniótica xenógena criopreservada em diferentes meios

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do cloridrato de dorzolamida a 2%, maleato de timol a 0,5% e associação de ambos na pressão intra-ocular: estudo experimental em cães

Pós-graduação em Medicina Veterinária - FMVZ

Avaliação ultrassonográfica do bulbo ocular em cães submetidos à facectomia por facoemulsificação com ou sem implante de lente intraocular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expressão dos antígenos nucleares de células em proliferação na superfície ocular de cães jovens e adultos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão gênica diferencial em resposta aos efeitos da proteína anti-inflamatória Anexina A1 nas células epiteliais pigmentadas da retina humana

Pós-graduação em Genética - IBILCE

Mensurações do bulbo ocular e cálculo do poder dióptrico de lentes intraoculares em coelhos (Oryctolagus cuniculus), LINNAEUS, 1758)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)