Use of uniform designs in combination with neural networks for viral infection process development

This work aimed to compare the predictive capacity of empirical models, based on the uniform design utilization combined to artificial neural networks with respect to classical factorial designs in bioprocess, using as example the rabies virus replication in BHK-21 cells. The viral infection process parameters under study were temperature (34°C, 37°C), multiplicity of infection (0.04, 0.07, 0.1), times of infection, and harvest (24, 48, 72 hours) and the monitored output parameter was viral production. A multilevel factorial experimental design was performed for the study of this system. Fractions of this experimental approach (18, 24, 30, 36 and 42 runs), defined according uniform designs, were used as alternative for modelling through artificial neural network and thereafter an output variable optimization was carried out by means of genetic algorithm methodology. Model prediction capacities for all uniform design approaches under study were better than that found for classical factorial design approach. It was demonstrated that uniform design in combination with artificial neural network could be an efficient experimental approach for modelling complex bioprocess like viral production. For the present study case, 67% of experimental resources were saved when compared to a classical factorial design approach. In the near future, this strategy could replace the established factorial designs used in the bioprocess development activities performed within biopharmaceutical organizations because of the improvements gained in the economics of experimentation that do not sacrifice the quality of decisions.

Devir - criança da matemática: experiências educativas infantis imagéticas

Pós-graduação em Educação Matemática - IGCE

Relações de trabalho dos jogadores profissionais do poker: um estudo à luz da psicossociologia

Pós-graduação em Psicologia - FCLAS

Differential cross section measurements for the production of a W boson in association with jets in proton-proton collisions at √s=7 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Long-range two-particle correlations of strange hadrons with charged particles in pPb and PbPb collisions at LHC energies

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Searches for supersymmetry using the M-T2 variable in hadronic events produced in pp collisions at 8 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Bose-Einstein correlation measurements at CMS

Multidimensional and one-dimensional quantum-statistical (Bose-Einstein) correlations are measured in proton proton collisions at 0.9, 2.76 and 7 TeV, in proton lead collisions at 5.02 TeV/nucleon pair and peripheral lead lead collisions at 2.76 TeV/nucleon pair center-of-mass energy with the CMS detector at the LHC. The correlation functions are extracted in terms of different components of the relative momentum of the pair, in order to investigate the extension of the emission source in different directions. The results are presented for different intervals of transverse pair momentum, k(T), and charged particle multiplicity of the collision, N-tracks, as well as for their integrated values. Besides inclusive charged particles, charged pions and kaons, identified via their energy loss in the silicon tracker detector, can also be correlated. The extracted source radii increase with increasing multiplicity, and decrease with increasing k(T). The results open the possibility to study scaling and factorization properties of these radii as a function of multiplicity, k(T), colliding system size and center-of-mass energy.

Potentiodynamic behaviour of CuA1Ag alloys in NaOH: a comparative study related to the pure metals electrochemistry

The mechanism of electrochemical oxidation of surface reformed CuA1Ag alloys having different composition of heat treatment, in 0.5 M NaOH was studied by means of cyclic polarization, constant potential electrolysis, ICP, AA, SEM and EDX. The surface reformation consisted of a repetitive triangular potential sweep (RTPS) between H 2 and O 2 evolution at 100 mV s -1 in the working solution itself, performed in order to increase the electrode roughness and obtain a quasi-stationary I/E profile in which the potentiodynamic behaviour of copper and silver was clearly revealed. The alloys suffer aluminum dealloying after such an RTPS. The quasi-stationary cyclic polarization curve exhibits a multiplicity of current peaks which have been related to the electrochemical reactions involving the pure alloying elements. Complex potential perturbation programmes in regions having different anodic and cathodic limits allowed the study of the mechanism of the electrochemical oxidation of the surface reformed alloys and the compare with that corresponding to the pure metals. The basic differences between the electro-oxidation processes of the surface reformed CuA1Ag alloys with respect to those established for the high purity alloying metals are the splitting of the peaks corresponding to the formation of the Cu(I) and Ag(I) species. © 1991.

Interleukin 10 and tumor necrosis factor-alpha in pregnancy: aspects of interest in clinical obstetrics

The purpose of this study was to review the literature regarding the action of the cytokines interleukin 10 (IL-10) and tumor necrosis factor-alpha (TNF-α) in pregnancy and to emphasize the factors that are of interest to clinical obstetrics. The literature highlights several actions of IL-10 and TNF-α during pregnancy. The actions of these cytokines seem to be antagonistic and dependent on the balance between them, which is orchestrated by the specific immunosuppressive action of IL-10. TNF-α has a characteristic inflammatory action, and it is an additional diabetogenic factor in pregnancy. The loss of the control of the production of these cytokines, with increase of TNF-α, is related to the risk for developing obstetric complications, particularly recurrent fetal loss, gestational diabetes mellitus, hypertensive syndromes, and fetal growth restriction. However, study results are controversial and are not clearly defined. These issues are attributed to the heterogeneity of the studies, particularly regarding their sample sizes and sources, the evaluation methods, and the multiplicity of factors and conditions that influence cytokine production. These questions are fundamental and should be addressed in future investigations to obtain more consistent results that can be applied to obstetric practice.

O que os homens têm a dizer sobre as mulheres? Os novos posicionamentos de jovens do gênero masculino frente às transformações femininas nas relações afetivas: uma leitura sob a ótica da Psicologia Analítica

Pós-graduação em Educação Sexual - FCLAR

A configuração de forças na constituição histórica do trabalho como estratégia de cuidado em saúde mental: problematizações foucaultianas

Investigated in this paper, as contributions to Foucault's genealogy of knowledge, the historical constitution of the joint activities between Work and Care in Mental Health (called Therapeutic Workshops, among other names) in their different purposes and dimensions that contributed to the production care of patients with psychological distress at different times. History is here understood as a multiplicity (and nonlinearity) of time series and various blending and shuffleforming speeches as truths. In conjunction we have discussed, we conclude that the work as a therapeutic modality in nursing homes, there emerged in the world of psychiatry, but of capitalism. Associated with measures of occupation "empty minds" of misfits, later earned the position of an instrument of discipline and social regulation. With the Psychiatric Reform, activities Work in Mental Health have been re-invented as spaces enablers of access to citizenship and contractuality social network users.

"...Depois, querida, ganharemos o mundo": reflexões sobre gênero, sexualidade e políticas públicas para travestis adolescentes, meninos femininos e outras variações

This article originated in a research that was meant to map out and examine the life conditions of “travesti” (transgender) teenagers and youth living in the city of Campinas, state of São Paulo. We contacted our informants outside the environments usually identifi ed as the common spaces where members of this group spend their time and share their experiences, as a strategy that we believed could enable us to better perceive how they construct their subjectivities. As we began our ethnographic endeavors, we were surprised by the multiplicity of ways in which these young people experimented with gender and sexuality, yet which were, at the same time, accompanied by eff orts to fi t these experiments within available identity categories. Feminine boys, cross dressers and drags thus constructed their own ways of living the feminine, and in doing so, stirred our imagination regarding contemporary experiences and political struggles in the realm of sexuality.

Efeitos da exposição gestacional, lactacional e juvenil às dietas com deficiência e suplementação de zinco e suscetibilidade a carcinogênese da mama em fêmeas Sprague-Dawley

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A representação social da infância veiculada no desenho animado Hora de aventura

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da ingestão de simbiótico e indol-3-carbinol sobre o processo de carcinogênese química de cólon em ratos Wistar alimentados com dieta contendo heme

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)