Estratégias biotecnológicas para a liberação controlada de antígenos e adjuvantes em vacinas através de lipossomas

Liposomes (LP) are colloidal systems with ability to compartmentalize therapeutic molecules in order to improve biological activity, decreases the potential toxicity, and to obtain prolonged effect. In this work it was discussed the role of the various liposomes types to encapsulate drug molecules able to provoke some immunological response (drugs, antigens and DNA). The effect of the liposomes and the parameters about the formation of the structures are also analyzed. Detailed literature review shows that, depending on the molecules polarity and the superficial charge of the liposome structures, the system may be efficiently used to optimize the therapeutic effects by means of the release control or through a drug delivery mechanism.

A reabilitação psicossocial no atendimento em saúde mental: Estratégias em construção

This study aims to identify the representations about Psychosocial Rehabilitation by Mental Health professionals working in open services, and also the difficulties they have met in the process of turning the care effective for the population. The study uses a qualitative methodology, collecting data by means of semistructured interviews with 15 subjects. The professionals identify the rehabilitation process as complex, meeting several obstacles and requiring their dedication and a flexible attitude to achieve the expected results.

Desempenho ponderal de novilhas mestiças Holandês x Zebu submetidas a duas estratégias de suplementação mineral

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estratégias de seleção dentro de progênies em duas populações de myracrodruon urundeuva Fr. All.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Tipificação das famílias de oito assentamentos rurais da região de Andradina (SP), com base em diferentes estratégias de produção e comercialização

This article intends to provide a typology of the families that are settled in the region of Andradina (Sao Paulo state), according to the main production and commercialization systems and to the presence (or not) of agricultural income diversification. The empiric data used to form the groups originate from socioeconomic questionnaires that had been applied to 169 families of eight rural settlements and also from 80 interviews. The groups were compared with each other, starting from the family's profile; patrimony; credit and technical support; self-consumption production; and the lot's external income. The strategy to increase the production and the productivity (dairy cattle) is limited to less than a quarter of the producers, however different strategies had been used by families, such as the diversification of the agricultural income and the direct sale to the consumer or to the retail trade. The association of the production diversification with the use, in a regular way, of differentiated channels of commercialization demonstrated a strategy capable to approximate the indicators of this type of producer comparing with those that focused on increasing the production and the productivity. This verification is important because, even though there is no necessary contradiction among both strategies, a part of the families does not have resources, infrastructure and/or technical qualification to follow the way of the productive intensification and others do not have this project as a priority.

Estresse oxidativo nos estágios finais da doença renal crônica em pequenos animais

The chronic kidney disease (CKD) it is characterized by irreversible structural lesions that can develop progressively for uremia and chronic renal failure (CRF). In the CRF it happens the incapacity of executing the functions of maintenance of the electrolyte balance and acid-base, catabolitos excretion and hormonal regulation appropriately. When the mechanism basic physiopathology of the renal upset is analyzed, it is observed that present factors, predispose to the unbalance oxidative. Most of the time, the renal patient comes badly nurtured, with lack in reservations of vitamins and minerals, what reduces the antioxidant defense mechanisms, what favors the installation of the renal oxidative stress, with the formation of species you reactivate of reactive oxygen species (ROS), substances these potentially harmful to the organism. The reduction of the glomerular filtration rate (GFR) in the evolution of CKD in dogs and cats is a component for the installation of the renal oxidative stress. The ROS possesses important action in the kidneys, and these substances are highly reactivate, and when presents in excess damage lipids, proteins, DNA and carbohydrate, driving functional and structural abnormalities taking the cellular apoptosis and necrosis. Against the harmful potential action of these substances you reactivate, she becomes fundamental a delicate control of his production and consumption in the half intracellular, in other words, a balance of his concentration intra and extracellular. That is possible due to the activity of the antioxidants. Like this, to present literature revision had as objective describes the participation of the oxidative stress in CRF, as well as the mechanisms defenses against the harmful action of those substances.

Análise de crescimento em clones de eucalipto submetidos a estresse hídrico

The aim of this study was to evaluate Eucaliptus grandis genotypes (Clones 105 and 433) in relation to drought tolerance, through growth plant analysis. Black PVC pots with 10 liter volume were used for cultivate plants in polyethilene greenhouse oriented east/west. Completely randonmized design with four treatments was used: two clones and two minimum soil water potentials (- 0.03 and -1,5 MPa) and sixteen replicates. Pots were weighed daily in order to evaluate water content and characteristic soli water curve was determined. Plant development was obtained each 15 days from planting until 60 days through total dry matter (DM), leaf area index (LAI), leaf area ratio (LAR), net assimilative ratio (NAR), specific leaf area (SLA), relative growth ratio (RGR) and absolute growth ratio (AGR). Results showed that clone 105 presented less sensibility to water deficit, which qualify it as genetic material for use under dry soil conditons. On the other hand, both clones had similar behavior with no water restrictions.

Características fotossintéticas de genótipos de capim-elefante anão (Pennisetum purpureum Schum.), em estresse hídrico

The experiment was carried out aiming to evaluate the gas exchange of dwarf elephant grass genotypes under different hydric conditions, in a randomized design with three replications. Genotypes of dwarf elephant grass (Mott, CNPGL 94-34-3 and CNPGL 92-198-7) were analyzed under two hydric conditions: irrigated (I) and non-irrigated (NI). Differences between treatments I and NI were observed for all genotypes for photosynthesis, stomatal conductance, transpiration and water vapor pressure deficit. Genotype CNPGL 94-34-3 presented highest tolerance to hydric stress, followed by Mott and CNPGL 92-198-7 genotypes. All genotypes presented high photosynthetic rate, under ideal conditions of soil humidity, thus characterizing the analyzed dwarf elephant grass genotypes as plants with high photosynthetic efficiency.

Determinação do índice de estresse hídrico em cultura do feijoeiro com termômetro de infravermelho

In order to calculate the daily stress degree of a bean crop submitted to four water regime applications, cover crop and air daily measurements temperatures were accomplished by using a hand-held infrared thermometer. The treatments did not present crop water stress except the control (without irrigation). The highest yield was obtained by the treatment that received less irrigation frequency, and among the treatments that had the same number of irrigation. The largest yield was obtained with the one that received larger amount of applied water. The largest irrigation frequency did not result in larger productivity. The methodologies used for the irrigation planning were efficient for the replacement of soil water. The daily stress degree index was effective in determining crop water stress; and it was reliable presenting negative values in good water soil condition.

Germinação e vigor de sementes de crambe sob estresse hídrico

This study aimed to evaluate crambe seeds germination and vigor under water stress conditions induced by polyethylene glycol solutions. The seeds were germinated on substrate moistened with polyethylene glycol solutions, using the osmotic potentials of 0 (control), -0.2, -0.4, -0.6, -0.8, -1.0, -1.2 and -1.4 MPa. The seeds germination was evaluated by normal seedlings and root emission percentages. Vigor was evaluated by germination average time, relative frequency, velocity and synchronization index. It was observed that more negative osmotic potential caused significant reduction in crambe seed germination and vigor, and no normal seedlings was observed at potentials below -0.6 MPa.

Avaliação do estresse oxidativo no sangue de consumidores habituais de suco de laranja

Two biomarkers of oxidative stress were evaluated in healthy volunteers treated with daily doses of orange juice that is well known source of vitamin C and citric flavanones, which have been associated with antioxidant, anti-inflammatory and hypolipidemic effects. The antioxidant capacity in the blood serum was evaluated by studying the scavenging of 2,2'-diphenyl-1-picrylhydrazyl (DPPH) free radical, and also lipid peroxidation was evaluated by the thiobarbituric acid reacting substances (TBARS) assay. The results have shown that the regular consumption of orange juice increased 150% and 200% the serum antioxidant capacity for women and men respectively, but it was no significant change in the serum lipid peroxidation. In conclusion, the increase of flavonones and vitamin C in the body due to the regular intake of orange juice expressively improved the antioxidant capacity, but without significant effect on the lipid peroxidation.

Fluorescência da clorofila-a e variação da simetria como ferramentas de investigação de plantas sob estresse

Environmental disturbances in various systems and individuals can result in a tendency for fluctuating asymmetry, which consists of a non-directional deviation from symmetry of a bilateral structure in a normally distributed population. Individuals unable to exhibit plastic responses to developmental disorders that occur at random, either genetic or environmental, may exhibit slight deviations from symmetry in organs with bilateral structure such as leaves. Thus analyzing plants by the morphological pattern of symmetry of the leaves has become an effective tool to describe the developmental of plants under stress conditions. This study aimed to analyze if the pattern of symmetry can be used as a tool to investigate the stress level to which a plant is subjected, using ultraviolet-C radiation (UV-C) as source of stress. We determined the leaf symmetry deviations (sd), the average length (AL) and the maximum width of the lamina (MWL) and the fluorescence parameters of chlorophyll-a in leaves of young plants of G. max subjected to 0, 30, 60, 120 or 240 minutes per week of UV-C during four weeks, submitting the values to ANOVA and Tukey test (p <0.05). The results showed significant reductions in AL and MWL, significant increase in sd in leaves irradiated and a drop in potential quantum yield of photosystem II (PSII) and in the photosynthesis performance index (IP). The analysis of leaf symmetry showed high correlation with the stress level to which plants were submitted, being an important analysis to ascertain the fitness of the plant because it is a noninvasive method and easy to perform.

Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro

To better understand the differences related to HS resistance between Bos indicus and Bos taurus, we aim to verify if the HS tolerance is due mostly to the genetic contribution from the oocyte, spermatozoa or both. Oocytes from Nelore and crossbreed Holstein cows (cHST) were collected, matured and fertilized with semen from Nelore (N), Angus (An), Brahman (Bra) and Gir (Gir) bulls. Nine six hours post insemination (hpi), ≥ 16 cells embryos were separated in two groups: control and HS. In control group, embryos were cultured at 39°C, whereas in the HS group, embryos were subjected to 41°C for 12 h, and then returned to 39°C. There was no effect of HS on blastocyst and hatched blastocyst rates in all breeds analyzed. The percentage of oocytes that cleaved and reached morula stage was significantly lower (p < 0.05) in cHST x Gir as compared to the other breeds. Additionally, blastocyst rates was higher in cHST x N than in cHST x An and cHST x Gir (p < 0.05). It was concluded that the oocyte is more important than the spermatozoa for the development of thermotolerance, since the breed of the bull did not influence embryo development after HS.

Avaliação dos níveis de frutosamina em gatos sob estresse agudo e crônico

Fructosamine are glycated serum proteins that are formed continuously due to the reaction between glucose and circulating proteins, and corresponding to the blood glucose control assessment over the last one to two weeks in cats. The fructosamine concentration has been used for differentiation between persistent and transient hyperglycemia. Therefore, the determination of fructosamine is considered the gold standard for monitoring glycemia into control in diabetic cats. The objective of this study was to evaluate the influence of acute and chronic stress of cats on serum fructosamine. 62 cats were selected from the Veterinary Hospital of FMVZ - UNESP, Botucatu campus. They were distributed into three groups: cats with a history of any illness or stress condition, excluding Diabetes Mellitus (DM), for a maximum of 48 hours (Group A, n = 21) or for a period exceeding 120 hours (Group B n = 27). The third group (Group C = control) was formed by 14 health cats. The groups were evaluated for serum fructosamine, glucose, protein and albumin. In this study, there was a significant increase in the values of fructosamine in animals subjected to acute and chronic stress, but these values remained within the reference range. The animals were, on average, normoglycemic, despite the positive correlation between fructosamine and glucose concentrations. We conclude that the fructosamine concentration is influenced by acute and chronic stress in cats, remaining, however, within the reference range, and therefore, still useful in the diagnosis of DM.

Morfofisiologia da cunhã cultivada sob estresse salino

The experiment was developed based on the importance of the nutritional factor cunhã for large and small, animals and on the growth of this forage in typical soils of the semiarid region, generally with salinity from natural or anthropogenic action. The experiment was performed in vessels with a capacity of 5kg prepared in a protected environment with full sunshine in the Department of Technology and Social Sciences (DTCS) of UNEB Campus III in Juazeiro - BA. It evaluated the effect of seven different salt concentrations on increasing levels of irrigation water electrical conductivity - ECw (0, 2, 4, 6, 8, 10 and 12 dS/m), at room temperature. The analysis of culture were taken every seven days observing the number of leaves, stem diameter, plant, plant length and total chlorophyll content at 13; 20; 27; 34 and 40 days after the start of irrigation with saline. The contents of proline, the ratio aero part/Root (AP/Rt), the root length and water content were evaluated in the 40 th day after the onset of treatment. The results showed that the elevation of ECw affected all variables.