Expressão do gene Glipican 3 (Gpc3) no urotélio da bexiga de ratos expostos ao diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) por 7 dias e 20 semanas

Diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a substituted urea herbicide widely used in crops of sugar cane, cotton and soybeans. In 1997, this agent has been classified by the United States Environmental Protection Agency as known/likely human carcinogen because it induced tumors in the urinary bladder and renal pelvis of rats, and breast and skin of mice exposed to 2500 ppm for feed for two years. A previous study from our group demonstrated dose-response relationship in the gene expression profile associated with severe necrosis on bladder urothelium and increased incidence of simple hyperplasia in male Wistar rats treated with different concentrations of diuron for 20 weeks. To check how early the molecular changes occurs, rats were fed for 7 days with diets containing diuron at 0, 125, 500 or 2500 ppm. The main observations recorded were urothelium ultrastructural alterations and disruptions of molecular pathways associated with cell-cell interaction and the tissue organization maintenance. Particularly, the gene Glypican 3 (Gpc3), a surface proteoglycan related to cellular adhesion and apoptosis induction, was down regulated on urothelium exposed to 2500ppm diuron for 7 days and 20 weeks. The aim of this study was validate by quantitative RT-PCR real time, the reduced Gpc3 gene expression in epithelial cells of the urinary bladder of male Wistar rats treated with different concentrations of diuron for 7 days and 20 weeks. The endogenous control of the quantitative PCR real time technique was the β-actin gene and the target was the gene Gpc3. The relative quantification (RQ) was obtained by the method of relative quantification 2-ΔΔCt . Animals exposed to diuron for 7 days or for 20 weeks presented reduction of Gpc3 gene expression compared to the control group. This reduction was statistically significant only for the 7 days study. Moreover, by comparing animals exposed for 7 days with the exposed for 20 weeks, it was ...

Mensagem, imagem e ideologias

O Projeto de pesquisa Mensagem, imagem e ideologias centrou seu foco principal na análise de figuras e signos ideológicos contidos nas placas e nos outdoors da cidade de Rio Claro-SP e região, no âmbito de divulgação de Instituições de Ensino. Pesquisamos os eixos transversais sobre a visão de educação e infância, a ponte entre os signos e o visual, as idéias contidas no processo das propagandas e o que se pretende vender com as imagens e os códigos expostos. Determinamos tais meios de comunicação, com propagandas de escolas de quase todos os níveis de escolaridade, para demonstrar e ilustrar as idéias contidas na aliança entre o capitalismo e a mídia, principalmente a fim de explicitar os fundamentos políticos, suas concepções e possíveis implicações na administração do processo educacional.

Análise de algoritmos computacionais utilizados em sistemas de planejamento de braquiterapia de alta taxa de dose: estudo comparativo com o Código MCNP-5C

In radiotherapy, computational systems are used for radiation dose determination in the treatment’s volume and radiometric parameters quality analysis of equipment and field irradiated. Due to the increasing technological advancement, several research has been performed in brachytherapy for different computational algorithms development which may be incorporated to treatment planning systems, providing greater accuracy and confidence in the dose calculation. Informatics and information technology fields undergo constant updating and refinement, allowing the use Monte Carlo Method to simulate brachytherapy source dose distribution. The methodology formalization employed to dosimetric analysis is based mainly in the American Association of Physicists in Medicine (AAPM) studies, by Task Group nº 43 (TG-43) and protocols aimed at dosimetry of these radiation sources types. This work aims to analyze the feasibility of using the MCNP-5C (Monte Carlo N-Particle) code to obtain radiometric parameters of brachytherapy sources and so to study the radiation dose variation in the treatment planning. Simulations were performed for the radiation dose variation in the source plan and determined the dosimetric parameters required by TG-43 formalism for the characterization of the two high dose rate iridium-192 sources. The calculated values were compared with the presents in the literature, which were obtained with different Monte Carlo simulations codes. The results showed excellent consistency with the compared codes, enhancing MCNP-5C code the capacity and viability in the sources dosimetry employed in HDR brachytherapy. The method employed may suggest a possible incorporation of this code in the treatment planning systems provided by manufactures together with the equipment, since besides reducing acquisition cost, it can also make the used computational routines more comprehensive, facilitating the brachytherapy ...

Estudo da contribuição do espalhamento nuclear não elástico na trajetória mais provável de prótons em alvo homogêneo composto de água

The Therapy with proton beam has shown more e ective than Radiotherapy for oncology treatment. However, to its planning use photon beam Computing Tomography that not considers the fundamentals di erences the interaction with the matter between X-rays and Protons. Nowadays, there is a great e ort to develop Tomography with proton beam. In this way it is necessary to know the most likely trajectory of proton beam to image reconstruction. In this work was realized calculus of the most likely trajectory of proton beam in homogeneous target compound with water that was considered the inelastic nuclear interaction. Other calculus was the analytical calculation of lateral de ection of proton beam. In the calculation were utilized programs that use Monte Carlo Method: SRIM 2006 (Stopping and Range of Ions in Matter ), MCNPX (Monte Carlo N-Particle eXtended) v2.50. And to analytical calculation was employed the software Wolfram Mathematica v7.0. We obtained how di erent nuclear reaction models modify the trajectory of proton beam and the comparative between analytical and Monte Carlo method

A leitura na perspectiva de Bakhtin e suas possibilidades na formação do leitor: na educação infantil e no primeiro ano do ensino fundamental

Overall, in Brazilian schools, the concept of reading does not take into account its possibilities and its meaning for life. But read is assigning meaning to something , make sense, interact with the text read. Thus, the present study addresses the mechanical reading, that without contextualization, performed on a technique by joining letters and syllables loose and also reading social use, consisting of statements, dialogism , polyphony and heteroglossy. It is known that the mechanical reading brings undesirable consequences, including learning problems, such as lack of attention, concentration, memory, imagination. When the reading is taught in a systematic way, with only the goal of the code decoding of written language, destroys social perspectives and intellectual development. It is common, when you offer the children a book, there the difficult disinterest of textual interpretation, because there is no attribute meaning to the content, and even the ability to interact with the text. With the interest in studying the dialogic reading this and the statements and genres, the present study was based on several authors: Bakhtin (1988, 2000), Schneuwly and Dolz (2000), Faraco (2009) and Ponzio (2009) Jolibert (1994), Bajard (2002; 2007) Foucambert (1997) Martins ( 1985) and Smith ( 1999). Aim for this study the following research question: what are the contributions of Mikhail Bakhtin's theory to the teaching of reading from kindergarten through the first grade of elementary school? Our hypothesis is that if they are guaranteed to pre -school and elementary school beginners diversified materials and new approaches in teaching reading and encouragement to her, will be developed in children a taste for the act of reading, so that they can assign meaning and significance, making reading part of their lives...

Análise de matérias primas e solução comercial empregada na perfusão e no transporte de órgãos destinados ao transplante

A solução HTK (histidina, triptofano e cetoglutarato) foi especialmente desenvolvida para preservar a heterogeneidade morfológica e bioquímica dos órgãos destinados ao transplante. Contém exclusivamente substâncias existentes no organismo tais como cloretos de sódio, potássio e magnésio, histidina, triptofano, manitol e cetoglutarato de potássio. Sua composição promove a inibição de todos os processos de ativação muscular, nervosa ou endócrina nas estruturas estimuláveis das membranas. Nos compêndios oficiais encontram-se métodos de análise qualitativa e quantitativa para matérias primas. Análises das matérias primas foram realizadas nos Laboratórios de Controle de Qualidade da faculdade de Farmácia e Laboratório de Eletroanalitica (NDcom) do Instituto de Química aplicando os métodos preconizados nos códigos oficiais. Os resultados obtidos com as matérias primas foram satisfatórios e confiáveis. Os mesmos métodos não possibilitaram a análise da Solução HTK acabada como proposto inicialmente. Esses métodos não apresentaram sensibilidade suficiente para a determinação das matérias primas empregadas no preparo desta solução, pois estas se encontram em concentrações bastante reduzidas, necessitando métodos mais sensíveis que realizem análise de traços de eletrólitos.

Estudo teórico da contribuição do espalhamento nuclear não elástico na trajetória de prótons de energias entre 50 e 250 MeV em materiais de interesse em radiologia: aplicação em tomografia com prótons

The goal of this work is to study the process of interaction of protons with matter through Monte Carlo simulation. For this purpose, it was employed the SRIM program (Stopping and Range of Ions in Matter ) and MCNPX (Monte Carlo N-Particle eXtended) v2.50. This work is going to support the development of a tomography system with protons. It was studied the interaction of proton with the follow materials: Polimethyl Mehacralate (PMMA), MS20 Tissue Substitute and water. This work employed energies in range of 50 MeV and 250 MeV, that is the range of clinical interest. The energy loss of proton after cross a material layer, the decreasing of its intensity, the angular and lateral de ection of incident beam, including and excluding nuclear interactions. This work is related with Medical Physics and Material Physics, like interaction of radiation with matter, particle transport phenomena, and the experimental methods in Nuclear Physics like simulation and computational by Monte Carlo method

Estatuto do índio, direito indígena e impacto ambiental: aldeias Guarani do Jaraguá (SP)

In Brazil, there are three main codes that surround the issue of right or possession of various lands according to concepts of ownership or purchase. When it comes to indigenous issues in Brazil is difficult to say which legal code applies more fairly the native population of the country. In the case of the Indians who have their reserves near urban areas there is such a conflict of laws becomes more evident and takes even greater than in other regions of Brazil. As is the case in the indigenous villages of the District of Jaragua in Greater São Paulo, the Tekoá YTU and Tekoá Pyau. The two villages are located in northeastern São Paulo and currently are surrounded by continuous growth and disorderly city of Sao Paulo while their inhabitants fight to preserve the customs and traditions of the Guarani people. They prevail on the City Statute (2001), the Indian Statute (EDI) Environmental Laws and the National Council of Environment (CONAMA) and the Forestry Code, the latter solely because they are near the State Park Jaragua - area environmental preservation

Produção de partículas secundárias em reações de “spallation” induzidas por prótons

Cosmic radiation has been identi ed as one of the main hazard to crew, aircraft and sensitive equipments involved in long-term missions and even high-altitude commercial ights. Generally, shields are used in spatial units to avoid excessive exposure, by holding the incident radiation. Unfortunatelly, shielding in space is problematic, especially when high-energy cosmic particles are considered, due to the production of large number of secondary particles, mainly neutrons, protons and alpha particles, caused by spallation reactions and quasi-elastic processes of the corpuscular radiation with the shield. Good parameters for checking the secondary particle production at target material are diferential cross section and energy deposited in the shield. Addition experiments, some computer codes based on Monte Carlo method show themselves a suitable tool to calculate shield parameters, due to have evaluated nuclear data libraries implemented on the algorithm. In view of this, the aim of this work is determining the parameters evaluated in shielding materials, by using MCNPX code, who shows good agreement with experimental data from literature. Among the materials, Aluminium had lower emission and production of secondary particles

Memória de onde, memória de quem

The spatial formation of Rio Claro city preserves several features of the problematic transition from the slave to remunerated labor in the late 20th, when the tacit ethnic bipolar sociability standard emerged, imprinting “black” and “white” forms to the city’s urban space. The ethnic social clubs José do Patrocínio and Tamoio, are two objects of ethnic groups’ exclusive use. Such ethnic social clubs indicate the existence of conflicts in their formation, as well as in their present existence, they are forms that preserve in themselves the codes and histories of the local black movement’s fight for their rights and for an autonomous place in the local society. In the present context the spaces are increasingly subjected to commercial interests and the spirits are embedded in confusion. What is the value of these spaces for the subjects who built them? The versions and narratives from oral accounts will allow us to understand the existing dimensions in the relation between the places and the memory, evidencing the importance of the patrimony and the patrimonial policies to the cities in the present historical period of geographic space globalization

Mil años de ciencia ficción hermética latinoamericana [1492-2500]: En tres episodios: Borges, la conspiración; Sor Juana y Antônio Vieira, íntimos herejes; Bizarros profetas ciberculturales

Pós-graduação em Letras - IBILCE

Busca quântica de dados XML usando algoritmo de Grover

This paper presents a quantum search algorithm implementation with small modifications. The algorithm idea is to be hybrid, capable to run on classical systems and quantum systems. We present the concepts of quantum search and introduced a pseudo-framework able to generate code for classical computers (C++) and quantum computers (QCL). The algorithms were submitted to simulations, which resulted in a comparative study of the operation of Grover’s algorithm on both systems, carrying out searches in a mass of data in XML format files. As a result, we see very similar numbers between classical and quantum systems, this creates an expectation that the search in real quantum computers is much more efficient.

Análise microestrutural de tubos API N80 soldados pelo processo HF/ERW para prospecção de petróleo e gás

On the grounds of the great advances achieved over recent years, the process HF/ERW (High-Frequency/Electric Resistance Welding)welded pipe have played an active role in the oil and gas industry for deep water applications, at high and extremely low temperatures, under high pressure and in highly corrosive environments, gradually replacing manufactured pipes by other processes. However, studies have shown that defects in the welded joints are a the leading causes of pipelines failures, which has required the determination of toughness values in this region, in compliance with the strict recommendations of the codes and standards with manufacturers and construction companies, on the oil and gas sector. As part of the validation process required toughness values, this research project focuses on a microstructural analysis in HF / ERW tubes microalloyed, steel grade API 5CT N80, designed to explore oil and gas in deep waters, the subject of strategic relevance to the country because of the recent discoveries in the Santos mega fields: Tupi and Libra (pre-salt). In this scientific work will be presented and discussed the results of mechanical tensile and Charpy, a few CTOD tests curves (showing the trend of toughness values to be obtained), and the microstructures of the base material obtained by optical microscopy, with special emphasis on the formation of non-metallic inclusions in the welded joint

Blogs para adolescentes e a articulação dos PCNs na produção escrileitora do ensino médio

The new National Curriculum of Basic Education in Brazil Parameters (PCNs- br) propose the curriculum reformulation of the High School in thematic areas encompassing competences and abilities. In this aspect the goal of this article is to verify the effects and meaning of the speeches posted on Folhateen's Blog (http://blogdofolha teen.folha.blog.uol.com.br/), to analyze if the practices of reading and writing, taken in this application, can be contemplated on the basic goals of the PCNs from the Languages area, codes and its technologies. It wonders of the using of media blogs for High School students as articulators of the Portuguese Language contents' study. It is possible to verify that the blogs offer its own languages and the expression of knowledge and power. They put the high school student before the articulation of several cross-cutting themes that promotes the interdisciplinarity. It points the need of a teacher's support so in a matted discursive voices the student won't be distracted, drift and paraphrase, but the interpretation and "new" methods of reading and polysemy. The theoretical framework is composed by the authors Foucault, Pecheux, Orlandi and Coracini.