Efeito da infestação de macrófitas aquáticas na comunidade planctônica em um viveiro de piscicultura

Pós-graduação em Microbiologia Agropecuária - FCAV

Reprodução de papagaio-verdadeiro (Amazona aestiva) em cativeiro: perfil anual de esteróides sexuais e ensaio de estímulo hormonal exógeno

Pós-graduação em Medicina Veterinária - FCAV

Variação temporal e espacial da taxa de sedimentação e das características limnológicas na microbacia do córrego da Barrinha, no município de Pirassununga, SP

Studies regarding the quality and quantity of water have become more and more important because of the increasing negative environmental impacts occurred and their scarcity increase as well. At the Center of Tropical Fish Research (Centro de Pesquisas de Peixes Tropicais - CEPTAlIBAMA) located in the city of Pirassununga, SP, there is a dam named Represa Velha which supplies 128 tanks that are fish hatcheries. This dam takes in part of the Barrinha stream micro-basin where it has been observed strong impacts due to the deforestation of its support forest, as well an the intensive agricultural activities in the neighboring areas. There has been established and distributed 13 points of water-collectors disposed inside the stream and the dam. The water samples were taken from the stream only from its surface because of the low depth and, inside. In the dam, the water samples were taken at the water column, at a 50cm distance over the sediment. In every point of collection inside the dam, it was placed a chamber of sedimentation, vertically in the water column at a 70% height from the total depth. The collections took place during the dry/cold and raining/warm periods of the years 2000 and 2001. Then, it were analyzed the physical, physical-chemical, chemical and micro-biological parameters of the water samples. The obtained results were statistically analyzed throughout the SAS system (Statistical Analisys System). Parameters such as total phosphate, nitrate, nitrite and turbidity were within the established limits for the Class 2 water, according to the n° 20/1986 CONAMA Resolução. However, the total and Escherichia coli coliforms, mainly inside the Barrinha stream, had showed values above of the established limits for the same... (Complete abstract click electronic access below)

Enzimas de biotransformação em tilápia (Oreochromis niloticus) exposta ao hormônio natural 17b-estradiol

Among the sexual hormones the estrogens are receiving major attention due to their biological activity. Such biological response is atributed to the best conformation recognized by their receptors, resulting in maximum responses. The estrogens are also considered responsible for most of disruptor´s effects caused by their presence in aquatic ecosystems. The 17β-estradiol hormone is produced by vertebrates during the reproductive phase of their lives and its presence has been detected in superficial waters. The objective of this study was to evaluate the cause-effect of tilapia exposition to the hormone 17β-estradiol through the analysis of biotransformation enzymes in liver and gills, complemented with the quantification of 17β-estradiol and estrone in water samples collected from fish ponds integrated to swine production. The present study was conducted under laboratory conditions, in a randomized experimental design with three levels of 17β-estradiol (E2) (0, 5, 15 µg L-1), with three replicates. After 7 days of exposure time, liver and gills were extracted to analyze three isoforms of cytochrome P450: EROD, BROD, PROD and the activity of Glutathione S-Transferase (GST). The results showed that the EROD activity (CYP1A), normally induced by the metabolism of aromatic compounds, did not present statistical differences among the treatments exposed to E2, what means that the hormone did not induce isoform 1A in fish under these particular experimental conditions. PROD activity was significantly altered in both concentrations, by means of 5 and 15 µg L-1, when compared to control. This result can indicate an important role of PROD on the metabolism of E2 present in water. Regarding to the BROD activity, it could be observed differences statistically significant between control and both groups of treatments. Two or more CYP isoforms can contribute to the metabolism of the same compound, what makes BROD a candidate as a next bioindicator of the exposure to E2 in aquatic ecosystems. Analysis of variance could confirm the effect of E2 statistically significant on the GST activity in liver tissues with >90% of significance (Prob>F = 0.0753). Furthermore, it was possible to observe that the values of GSTs activities in liver and gills in both, control and treatments, follow a tendency, that means, enzymatic activity in gills increase as the increasing of the activity in the liver tissues. In this study, the 17β–estradiol was found in measurable concentrations in three sampled points, and these values were similar to the findings of other authors at different locations in Brazil. In addition, those values are much higher than the minimum concentration that presented observable effects (10 ng L-1).

Armazenamento de sementes germinadas de Handroanthus heptaphyllus (Vell.) Mattos e seu comportamento em viveiro

Pós-graduação em Ciência Florestal - FCA

Seleção fenotípica e identificação de snps em regiões genômicas ligados a resistência à ferrugem em Eucalyptus grandis

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Inter-relações entre nematoides, fungo e a cultura da seringueira

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Expression of Vascular Endothelial Growth Factor (VEGF) in Macrophages, Fibroblasts, and Endothelial Cells in Pterygium Treated with 5-Fluorouracil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Histochemical and morphological features of biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga, Brycon nattereri (Characiformes)

The aim of the present study was to characterize biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga (Brycon nattereri) using histochemical and morphological analyses. Biopsied oocytes had a mean diameter of 2.225 mm (modal diameter: 2.312 mm), complete vitellogenesis and a central or slightly eccentric nucleus. Neutral polysaccharides were detected in the follicular cells, zona radiata and yolk globules, while acidic polysaccharides were detected in the follicular cells and cortical alveoli. Ten out of the 19 females treated with two doses of carp pituitary extract (cPE) released oocytes, which were also analysed. Stripping occurred 292 +/- 39 degree-hours after the second dose of cPE and led to a mean spawning weight of 36.2 g, 10% spawning index, 241 oocytes/g of ova, 8222 oocytes/female and 23 oocytes/g of body weight. Stripped oocytes had a mean diameter of 2.33 mm and a mode at 2.375 mm, were weakly adhesive and coloration ranged from wine to brown. Under scanning electron microscopy, stripped oocytes exhibited a single funnel-shaped micropyle located at the animal pole and a zona radiata that measured 7.7 m in thickness with eight pore canals/m(2). Oocyte morphology in Brycon nattereri is similar to that found in other species of the genus, except for the larger size and weaker adhesiveness. These findings provide essential information for a better understanding of the reproductive biology of B. nattereri and the establishment of conservation measures for this threatened species.

Initial growth of bauhinia variegata trees under different colored shade nets and light conditions

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of salinity on the metabolism and osmoregulation of selected ontogenetic stages of an Amazon population of Macrobrachium amazonicum shrimp (Decapoda, Palaemonidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In vitro study of cyclosporine A 0.05 % on primary and recurrent pterygium fibroblasts

To compare the cyclosporine 0.05 % exposure effect on fibroblasts from primary and recurrent pterygium. Primary culture of fibroblasts from primary and recurrent pterygium was performed until the third passage, which was exposed to cyclosporine 0.05 % in a group and the other remaining unexposed (control group), in triplicates. After 3, 6, 12, and 17 days of exposure the viable cell counting was performed by hemocytometer. The results were statistically analyzed using the technique of analysis of non-parametric variance model for repeated measures with three factors. There was a significant reduction in both fibroblast proliferation, in primary as in the recurrent pterygium cultures exposed to cyclosporine when compared not exposed cultures, with statistical significance (P < 0.05). Comparing primary and recurrent pterygium that received the drug, there was no significant difference in cell proliferation in relation to primary or recurrent pterygium. Cyclosporine 0.05 % is effective in inhibiting fibroblast proliferation in culture, both in primary and as in recurrent pterygium.

Utilização de redes neurais artificiais aplicadas na discriminação de padrões de doenças florestais

Pós-graduação em Ciência Florestal - FCA

Viabilidade do recipiente biodegradável na produção de mudas florestais nativas

Pós-graduação em Ciência Florestal - FCA

Avaliação das condições bióticas e abióticas em um viveiro neotropical impactado

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)