Avaliação quantitativa da insuficiência da veia safena magna nos pacientes portadores de varizes primárias de membros inferiores pelo mapeamento dúplex e fotopletismografia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo farmacocinético e farmacodinâmico de cloridrato metadona por via oral, intramuscular e intravenosa e carreadores lipídicos nanoparticulados de metadona via oral em equinos

Pós-graduação em Anestesiologia - FMB

Sistema multiparticulado magnético: desenvolvimento e avaliação In Vivo através da biossusceptometria AC

Pós-graduação em Ciências Farmacêuticas - FCFAR

Determinação da concentração alveolar mínima(CAM) de sevofluorano em filhotes de cães (neonatos e pediátricos)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise e extração de características de imagens termográficas utilizando componentes principais

Pós-graduação em Engenharia Mecânica - FEIS

Ultrassonografia transcraniana em cães hígidos: padronização da técnica e descrição anatômica

The study of the nervous system is increasing in Veterinary Medicine. Transcranial ultrasonography (TCUS) has the advantage of being a non-invasive and low cost method compared to computerized tomography (CT) and Magnetic Resonance Imaging (MRI). The temporal bone has been used as an acoustic window in TCUS in humans. This study aimed to correlate transcranial ultrasonographic images obtained through the temporal and occipital window with healthy dog's encephalic anatomy, and to standardize the technique. 37 adult mongrel dogs were used: 30 animals in vivo, in order to perform USTC screening and seven dog corpses for brain section as well as USTC planes. Data analysis was accomplished by the non parametric Wilcoxon test. Results obtained indicate that TCUS (in dorsal and oblique planes) is a viable method for brain evaluation in dogs weighting up to 10kg without anesthesia.

Scale morphology of Prochilodus lineatus with emphasis on the scale epithelium

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterisation of the methylation pattern in the intragenic CpG island of the IGF2 gene in Bos taurus indicus cumulus cells during in vitro maturation

The aim of this study was to characterise the methylation pattern in a CpG island of the IGF2 gene in cumulus cells from 1-3 mm and a parts per thousand yenaEuro parts per thousand 8.0 mm follicles and to evaluate the effects of in vitro maturation on this pattern.Genomic DNA was treatment with sodium bisulphite. Nested PCR using bisulphite-treated DNA was performed, and DNA methylation patterns have been characterised.There were no differences in the methylation pattern among groups (P > 0.05). Cells of pre-IVM and post-IVM from small follicles showed methylation levels of 78.17 +/- 14.11 % and 82.93 +/- 5.86 %, respectively, and those from large follicles showed methylation levels of 81.81 +/- 10.40 % and 79.64 +/- 13.04 %, respectively. Evaluating only the effect of in vitro maturation, cells of pre-IVM and post-IVM COCs showed methylation levels of 80.17 +/- 12.01 % and 81.19 +/- 10.15 %.In conclusion, the methylation levels of the cumulus cells of all groups were higher than that expected from the imprinted pattern of somatic cells. As the cumulus cells from the pre-IVM follicles were not subjected to any in vitro manipulation, the hypermethylated pattern that was observed may be the actual physiological methylation pattern for this particular locus in these cells. Due the importance of DNA methylation in oogenesis, and to be a non-invasive method for determining oocyte quality, the identification of new epigenetic markers in cumulus cells has great potential to be used to support reproductive biotechniques in humans and other mammals.

Eye darkening as a reliable, easy and inexpensive indicator of stress in fish

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Short interspersed CAN SINE elements as prognostic markers in canine mammary neoplasia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estimating outcomes in newborn infants using fuzzy logic

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estrutura do esmalte humano e sua interface com sistemas adesivos

Esta tese sistematiza uma série de estudos dedicados à compreensão da interface do esmalte e sistemas adesivos. Para tanto, a estrutura da camada aprismática do esmalte em alta resolução pela microscopia eletrônica de varredura foi avaliada quanto a sua continuidade, espessura, distribuição nas faces coronárias e em fossas e fissuras, predominantemente em dentes decíduos não erupcionados. Foi também analisado pela microscopia eletrônica de varredura os efeitos do condicionamento de diferentes tipos de ácido e, em especial, do ácido fosfórico em diferentes concentrações, metodologias e tempos de aplicação. Estes trabalhos permitiram visualizar as principais ações ácidas desmineralizantes e os tipos de retenções no centro ou periferias dos prismas ou em ambas as regiões, bem como a profundidade e forma dos “tags” resinosos com carga e sem carga na intimidade do esmalte. No caso particular da aplicação dos selantes em fossas e fissuras foi também avaliada comparativamente a eficácia das técnicas invasivas ou não invasivas. Os últimos experimentos foram dedicados ao estudo da microinfiltração de diferentes sistemas adesivos e da resistência destes sistemas adesivos na estrutura do esmalte. Novos estudos merecem dar continuidade à linha de pesquisa de sempre priorizando o propósito de vincular os procedimentos aplicados com uma consistente e indispensável fundamentação de pesquisa básica no interesse da clínica odontopediátrica.

Ultrasonographic measurements of kidney fat thickness and Longissimus muscle area in predicting body composition of pregnant goats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Valor de corte para teste respiratório com isótopos estáveis (Carbono 13) no diagnóstico do Helicobacter pylori utilizando IRMS para análise do sopro

After the isolation of Helicobacter pylori from an injury at the stomach mucosa by Marshall and Wareen, work that was recognized with the Nobel Prize of Medicine or Physiology in 2005, many other works showed the relationship between the presence of H. pylori and diseases at the digestive system, such as gastritis, gastric, duodenal and peptic ulcer, and stomach cancer. The 13C-Urea Breath Test - 13C-UBT is a non-invasive diagnostic method that utilizes the breath of a patient to determine the presence of H. pylori through stable isotopes. This work aimed to find an ideal 13C-UBT Isotopic Ratio Mass Spectroscopy cut-off value (a threshold between positive and negative) to diagnose H. pylori infection at Brazilian population. Patients were selected at the UNESP-Botucatu Clinical Hospital Endoscopy Section. With these results it was possible to indicate that the best cut-off value is between 2.5 to 6 ‰ of Delta Over Baseline (DOB)

Síndromes paraneoplásicas hematológicas em cães

Tumoral masses can cause several direct problems in the organism, such as invading organs and altering their functions, leading to other problems such as the Paraneoplastic syndrome. The paraneoplastic syndrome is an alteration in the structure and function of the body due to the non-invasive actions of the tumor, for example, liberating hormones, peptides, cytokines and leading to cross reactions between normal tissues. The syndrome can affect different locations in the body, being that some are indicative of specific tumors, however the interpretation of the clinical and pathological findings referring to this syndrome should be utilized in the diagnostic and treatment. In the hematological paraneoplastic syndrome, there are alterations that occur due to indirect actions of the tumor on the blood cellular elements and the coagulation system, and that are generally detected in routine clinical and laboratorial exams. This study objective is to aboard some hematological paraneoplastic syndromes in dogs such as: anemia, thrombocytopenia, neutrophilic leukocytosis, hypergammaglobulinemia and erythrocytosis, emphasizing the different etiologies that may assist in differential diagnosis and the principal neoplasm related to this syndrome. Besides this, the early discovery and treatment of the paraneoplatic syndrome is important as the tumor itself, as it improves the prognostic and quality of life of the patient