Hydrogen peroxide centrally attenuates hyperosmolarity-induced thirst and natriuresis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Compact setup for reflection holography with Bi12TiO 20 crystals

A novel optical setup for imaging through reflection holography with Bi12TiO20 (BTO) sillenite photorefractive crystals is proposed. Aiming a compact, robust and simple optical setup the lensless Denisiuk arrangement was chosen, using a He-Ne red laser as light source. In this setup the holographic medium is placed between the light source and the object. The beam impinging the crystal front face is the reference one, while the light scattered by the surface is the object beam in a holographic recording by diffusion. In order to allow the readout of the diffracted wave only and to keep the setup simplicity a polarizing beam splitter cube (PBS) was positioned at the BTO input. The reference beam is s-polarized (polarization direction perpendicular to the table top) and the crystal. 〈001〉-axis is rotated by an angle γ with respect to the input polarization in order to make the transmitted object beam and the diffracted beam to have orthogonal polarizations. While the transmitted wave is reflected by the PBS at a right angle, the diffracted wave carrying the holographic reconstruction of the object passes through the PBS, being collected by a positive lens in order to form the holographic image at a CCD camera. The holographic recording with the grating vector is parallel to the 〈100〉-axis. An expression for the diffracted wave intensity as a function of γ was derived, and this relation was experimentally investigated. © 2008 American Institute of Physics.

A substituição do meio TCM pelo meio BARC, suplementado com SFB, BSA ou PVA, para maturação de oócitos bovinos in vitro, não incrementa a subsequente produção de blastocistos

This study was carried out to assess the influence of bovine embryo culture medium Beltsville Agriculture Research Center (BARC), supplemented with FCS, BSA or PVA, on the in vitro oocyte maturation, evidenced by cleavage rate and blastocysts production at different developmental stages. Three experiments were performed, as follows: exp.1: addition of FCS to BARC medium at concentrations of 0, 5 and 10%; exp. 2: addition of BSA to BARC medium at concentrations of 0, 4 and 8 mg/ml; exp. 3: addition of PVA to BARC medium at concentrations of 0, 0.5 and 1.0 mg/ml. TCM 199 supplemented with bicarbonate, pyruvate, gentamicin sulfate, FSH, LH and FCS was used as control group. Oocytes obtained from cow ovaries at slaughterhouse were selected in PBS, and then matured in BARC medium supplemented with FSH, LH and gentamicin sulfate, according to the experimental design. Percoll gradient was used for sperm selection and TALP medium for IVF. In vitro embryo culture was in SOF-m medium; a humidified atmosphere with 5% CO2, in air, at 38.7oC was used for all steps. The number of oocytes reaching blastocyst, expanded blastocyst, and hatched blastocyt stages was recorded, respectively at 72 and 168 h post-insemination. ANOVA and Bonferroni t test were used to determine differences among groups. Differences of P<0.05 were taken as significant. Higher percentage (P<0.05) of cleaved oocytes was observed in group TCM + FCS than for the other groups matured in BARC supplemented with FCS or BSA, regardless the concentration used. However, the cleavage rate was similar between groups BARC plus PVA with 1 mg/ml (85.7%) and TCM + FCS (90.8%). Significant difference was found among groups for the production of blastocysts, with the control group yielding a higher number of blastocysts (results ranging from 47.4 to 51.4%, in comparison with groups using BARC + FCS (4.1 to 19.7%), BSA (1.4 to 5.6%) and PVA (5.7 to 10.6%). In conclusion, BARC medium supplemented with different macromolecules did not promote a beneficial effect on in vitro oocyte maturation, resulting in lower rate of cleavage and blastocyst production when compared with TCM + FCS medium.

Efeito da inibição dos receptores canabinóides CB₁ ou CB₂ durante o período neonatal sobre o dimorfismo sexual esquelético ao longo do desenvolvimento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da resposta inflamatória local após aplicação de células tronco mesenquimais alogênicas em tendão flexor digital superficial de equinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do benzoato de estradiol na recuperação de oócitos, embriões e nos parâmetros hematológicos de cadelas recém acasaladas

We investigated estradiol benzoate effects on oocytes/embryos recovery rate and the influence of this drug on the hematopoietic system. Twenty four bitches were divided in two groups, Group I, 12 females that received a single shot of estradiol benzoate, 0.2 mg/kg intramuscularly, between 2 and 7 days after the date of the last mismating or insemination and, Group II (control), 12 bitches that received 0.2 ml/kg of oily diluent, in corresponding dates. The bitches were ovary-hysterectomized and the uterus/oviduct were isolated and flushed with a PBS, heparin and polyvinyl alcohol solution. Oocytes and embryos were quantified and classified according to their stage of development. Blood counts were performed on M1 (before drug administration), M2 (15 days after drug administration) and M3 (40 days after drug administration). Pearson correlation coefficient was used to analyze the variable retrieval structures, while Fisher exact test was used for the analysis of embryonic viability. ANOVA was used to analyze repeated measurements and Tukey test for hematological parameters. All tests were performed at 5% significance level. The recovery rate of total structures in group I was lower (22.88%) than group II (65.85%). A lower embryo recovery (ratio 3: 52) rate and a greater number of degenerated structures (ratio 11: 1) were observed in group I. Hematological parameters showed significant difference in erythrocytes, hematocrit and hemoglobin concentrations 15 days after drug administration and difference in leukocytes concentration 40 days after using the medication in bitches of group I, however, at the end of the experiment all bitches had blood counts considered normal.

Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos

Fundação de Apoio à Pesquisa do Estado de São Paulo (FAPESP)

Apoptose em placentônios bovinos de gestações de conceptos naturais e de transgênicos clonados

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Modulação da doença periodontal por curcumin modificado quimicamente. Estudo de dose-resposta em roedor

Pós-graduação em Odontologia - FOAR

Inflamassomos NLRC4 E NLRP3 na Doença Periodontal Experimental Induzida por Aggregatibacter Actinomycetemcomitans

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização da liga Ti-30Ta após tratamento biomimético associado à incorporação de antibiótico

Surface treatments have been used to modify the surface of titanium alloys. The purpose of this study is to evaluate the surface of Ti-30Ta alloy after biomimetic approach associated to antibiotic incorporation. The ingots were obtained in arc melting furnace, treated and cold-worked by swaging. The surface treatment was performed in two steps: biomimetic treatment and antibiotic incorporation. For biomimetic treatment, first an alkaline treatment (NaOH 1M at 60ºC) was performed, followed by heat treatment and immersion in SBFx5 (Simulated Body Fluid) for a period of 24 hours. In order to incorporate the antibiotic, samples were immersed in a solution formed by drugs plus SBFx5 for 48 hours. The sample surfaces were analyzed by scanning electron microscopy (SEM), X-Ray diffraction (XRD), atomic force microscopy (AFM) and contact angle measurements. The release of antibiotic from coated implants was measured in phosphate buffer saline at pH 7.4 by using UV/VIS spectrometry. Results have shown changes on the surface after incorporating the drug, which is gradually co-precipitated with the Ca-P crystals, forming a uniform and rough layer on the metal surface