Macrophage migration inhibitory factor and oral cancer

Background: Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine with pro-inflammatory functions and involved in tumorigenesis. The aim of this study was to evaluate the expression and localization of the macrophage MIF in oral squamous carcinoma (OSC). In addition, the relationship between MIF expression and clinicopathological parameters such as survival data, tobacco use, alcohol habits, TNM stage, tumor graduation, and peritumoral inflammatory infiltrate were evaluated. Methods: Using immunohistochemistry, expression and localization of MIF was detected in 44 specimens of OSC. The absolute number and relative proportions of MIF-positive cells detected were also determined separately for tumor parenchyma vs. stroma. All counts were determined from 10 consecutive high-power fields using an integration graticule. Moreover, some parameters were analyzed separately for lip and intra-oral cancers. Results: Migration inhibitory factor-positive cells were observed in both the tumor parenchyma and in inflammatory cells of all specimens. In contrast, MIF expression was not detected in tumoral nests associated with poorly differentiated tumors. In specimens of lip cancer, a greater number of MIF-positive stromal immune cells were detected than in intra-oral cancer specimens (Mann-Whitney test, P = 0.049). Conclusions: Oral squamous carcinoma cells consistently express MIF independent of their location. Lip tumors presented more MIF-positive peritumoral inflammatory cells, similar to control, suggesting that immunological differences in leukocyte activation exist between in lip and intra-oral cancers. © 2012 John Wiley & Sons A/S.

Liposomal-praziquantel: Efficacy against Schistosoma mansoni in a preclinical assay

Currently, schistosomiasis mansoni is treated clinically with praziquantel (PZQ). Nevertheless, cases of tolerance and resistance to this drug have been reported, creating the need to develop new drugs or to improve existing drugs. Considering the small number of new drugs against Schistosoma mansoni, the design of nanotechnology-based drug delivery systems is an important strategy in combating this disease. The aim of this study was to evaluate the activity of PZQ containing liposome (lip.PZQ) on S. mansoni, BH strain. Mice were treated orally with different concentrations of PZQ and lip.PZQ 30 and 45 days following infection. The number of worms, recovered by perfusion of the hepatic portal system, and the number of eggs found in the intestine and liver were analysed. Parasite egg counts were also performed. The most active formulation for all parameters was 300. mg/kg of lip.PZQ, since as it decreased the total number of worms by 68.8%, the number of eggs in the intestine by 79%, and the number of hepatic granulomas by 98.4% compared to untreated controls. In addition, this concentration decreased egg counts by 55.5%. The improved efficacy of the treatment with lip.PZQ, especially when administered 45 days following infection, compared with the positive-control group (untreated) and the groups that received free PZQ, can be explained by greater bioavailability in the host organism; the preferred target of lip.PZQ is the liver, and lip.PZQ is better absorbed by the tegument of S. mansoni, which has an affinity for phospholipids. © 2013 Elsevier B.V.

Respiração freno labial em indivíduos com doença pulmonar obstrutiva crônica: revisão da literatura e influência sobre a modulação autonômica cardíaca

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise clínico-histológica da queilite actínica: uma abordagem interexaminadores e correlação de áreas atróficas e não atróficas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seleção de espécies de basidiomicetos produtoras de ligninases para caracterização e aplicação das enzimas sobre corantes aromáticos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alterações cefalométricas do perfil facial decorrentes do crescimento natural e induzidas pelo aparelho de Herbst no tratamento da classe II divisão 1, em fase pré-puberal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação estética do lábio nos portadores de fissura pré-forame incisivo unilateral completa, submetidos a queiloplastia primária com a técnica de Spina

Pós-graduação em Bases Gerais da Cirurgia - FMB

Etiologia e susceptibilidade à drogas de microrganismos relacionados com a otite média crônica supurativa em pacientes portadores de fissura palatal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo das necessidades, planejamento e prognóstico endodôntico em pacientes com fissuras de lábio e/ou palato submetidos ao tratamento cirúrgico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Linhagens fúngicas na hidrólise enzimática de bagaço de cana-de-açúcar

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Ensino de Física para pessoas surdas: o processo educacional do surdo no ensino médio e suas relações no ambiente escolar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção do papilomavírus humano (HPV) em carcinoma epidermóide de lábio através da nested PCR e sua correlação com os fatores de risco, características clínicas, anatomopatológicas e de sobrevida

Pós-graduação em Odontologia - FOA

Avaliação das alterações tegumentares produzidas pelo aparelho de Herbst em indivíduos classe II divisão 1º, tratados em diferentes estágios de crescimento

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Alterações do perfil médio facial no tratamento da má oclusão de classe II, divisão 1 de Angle com aparelho extrabucal de Kloehn

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação cefalométrica das alterações labiais de indivíduos dolicocéfalos portadores de má oclusão classe III, tratados com cirurgia bimaxilar

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)