Water quality and communities associated with macrophytes in a shallow water-supply reservoir on an aquaculture farm

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparative analysis of the replication of bovine herpesvirus 1 (BHV1) and BHV5 in bovine derived-neuron-like cells

Members of the subfamily Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as preferential sites for primary replication. However, bovine herpesvirus 5 (BoHV5) is neurotropic and neuroinvasive and responsible for meningoencephalitis in cattle and in animal models. A related virus, BoHV1 has also been occasionally implicated in natural cases of neurological infection and disease in cattle. The aim of the present study was to assess the in vitro effects of BoHV1 and BoHV5 replication in neuron-like cells. Overall, cytopathic effects, consisting of floating rounded cells, giant cells and monolayer lysis, induced by both viruses at 48 h postinfection (p.i.) resulted in a loss of cell viability and high virus titres (r = 0.978). The BoHV1 Cooper strain produced the lowest titres in neuron-like cells, although viral DNA was detected in infected cells during all experiments. Virus replication in infected cells was demonstrated by immunocytochemistry, flow cytometry and qPCR assays. BoHV antigens were better visualized at 48 h p.i. and flow cytometry analysis showed that SV56/90 and Los Angeles antigens were present at higher levels. In spite of the fact that BoHV titres dropped at 48 h p.i, viral DNA remained detectable until 120 h p.i. Sensitive TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) and annexin V assays were used to identify apoptosis. BoHV5 induced death in approximately 50 % of cells within 24 h p.i., similar to what has been observed for BoHV1 Los Angeles. Infection with the BoHV1 Cooper strain resulted in 26.37 % of cells being in the early stages of apoptosis; 63.69 % of infected cells were considered viable. Modulation of mitochondrial function, as measured by mitochondrial membrane depolarization, was synchronous with the virus replication cycle, cell viability and virus titres at 48 h p.i. Our results indicate that apoptosis plays an important role in preventing neuronal death and provides a bovine-derived in vitro system to study herpesvirus-neuron interactions.

Percepção do cirurgião-dentista sobre formação profissional e dificuldades de inserção no mercado de trabalho

Introduction: The evaluation of the perception of graduates is an essential strategy to support the political project educational in universities. Objective: This study aimed to verify the perception of dentists which are working in the labor market, about the difficulties of professional insertion after the graduation, positives and negatives aspects and suggestions regarding the training received. Methodology: This is a qualitative survey conducted with graduates of the years 2000 to 2010, of dentistry course from a Brazilian Public University. A self-administered questionnaire was sent by mail/email to all graduates in the period. The data were transcribed and subjected to content analysis, divided into pre-analysis, characterized by carefully reading floating, material exploration, guidelines for finding the analysis itself. Result: The categories related to the difficulties in early life were: getting a job/workplace, adequate working conditions and low pay, insecurity and confrontation obtained the academic and lack of administrative experience. The perceived positive aspects for the training were: good infrastructure and reputation of the university, integrating teaching-research, teacher-student relationship. The negative aspects comprised the following categories: administrative preparation, lack of integration between content theoretical/ practical, concepts used in the labor market. The suggestions presented are consistent with the difficulties to the beginning of professional life and the negative aspects. Conclusion: The professionals have faced the saturation of the labor market and different reality from those found in academic life. Despite the positive aspects, graduates suggest changes in the course conducted.

Selección del tratamiento farmacológico en el estado de mal epiléptico en niños

The status epilepticus (SE) is characterized by a series of crises which occur without recovery of consciousness or a single seizure lasting more than 30 minutes and can damage the central nervous system and systemic. The duration and frequency of attacks are directly related to patient prognosis. Whether seizures occur often and are longer, increase the risk of neurological and systemic complications. Child population has a higher frequency of seizures and it is associated with a lower threshold of immature brain to trigger these episodes. Aim: To determine the safest drugs prescribed for children in SE, their doses and schedules. A bibliographic survey was performed in electronic databases. Methods: The scientific health descriptors used for search was: “status epilecticus” and “anticonvulsivants” and “child”. Results: This strategy identified 396 manuscripts, of whom four were considered eligible for the study, after the assessment by floating reading and criteria. Of these, two were randomized trials and two descriptive. The studies address the use of oral midazolam, rectal diazepam and intravenous lorazepam. Conclusions: All investigated drugs were effective in treat in status epilepticus. Lorazepam is highlight, since it has fewer secondary effects and, as an alternative to the intravenous, oral midazolam. However, there is a need for further studies to demonstrate the efficacy and safety in the use of drugs in children.

Análise de redes sociais e aspectos financeiros da produção de tilápias em tanques-rede na uhe de Ilha Solteira

Pós-graduação em Agronegócio e Desenvolvimento - Tupã

Influência das temperaturas constantes e flutuantes sobre as características biológicas de Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) em gerações sucessivas

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Projeto de um tanque de armazenamento atmosférico com teto flutuante para estocagem de gasolina

In this paper are discussed the main project stages of an atmospheric storage tank with floating roof. It emphasizes the calculations of the bottom, the shell and the floating roof, discussing its stages and conditions, based on the technical standards of the project. The storage tanks are static equipment working under low pressure and has an essential importance for the operation of a process plant. This paper has the purpose of obtaining the dimensions and materials of each component of the tank through the calculations and prove them with results obtained by numerical simulation by finite elements with the help of Autodesk Inventor software, consolidating the project