757 resultados para digestibilidade de aminoácidos


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Realizou-se um estudo para comparar a técnica de degradação in vitro e digestibilidade in vivo, para a qual foram utilizados oito ovinos capões que receberam feno de capim-de-rhodes (Chloris gayana Kunt.) e capim-green panic (Panicum maximum Jacq. var. Trichoglume cv. Petrie),em quatro estágios de maturação. No estudo da digestibilidade in vivo não se verificaram diferenças significativas entre os diferentes coeficientes de digestibilidade dos alimentos. Para a degradação in vitro da fibra em detergente neutro com 24 a 48 horas, não houve diferenças significativas (P > 0,05) para os mesmos nutrientes. Determinaram-se as equações de regressão e os coeficientes de correlação. Os valores de r foram considerados de médios a elevados, e os erros padrões da estimativa, satisfatórios a elevados. O maior valor de correlação foi obtido entre a taxa de degradação relativa de 24 a 48 horas, e o teor de NDT das forragens (r= 0,84), com erro-padrão da estimativa satisfatório (Sy’x = 3,92).

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Three steers equipped with ruminal and duodenal cannulas were fed roughage:concentrate ratios 80:20, 60:40 and 40:60 in order to study intake and apparent, rumen and post-rumen digestibilities. The roughage was ''coast cross'' (Cynodon dactylon) hay (5.67% CP and 83.30% NDF). Undigestible neutral detergent fiber (NDF) was used as dry matter (DM) flow marker. DM intake means were 77.99, 91.03 and 91.81g DM/kg BW0.75, for the 20, 40 and 60% concentrate diets, respectively. DM intake for the 20% diet was statistically (P < 0.05) different from the other two diets. Apparent digestion coefficient (%) of DM (50.48, 57.32 and 61.33), organic matter (OM) (52.03, 58.91 and 62.76) and gross energy (GE) (48.95, 56.40 and 60.00) increased significantly with the increase in concentrate ratio of the diets. For the following components the apparent digestion coefficients were not statistically different: NDF (44.54, 45.28 and 42.53), ADF (40.69 44.39 and 43.60), cellulose (51.54, 54.34 and 52.03), hemicellulose (49.63, 46.78 and 39.18) and starch (86.59, 91.89 and 93.21). DM, OM, NDF, ATF, cellulose and starch ruminal and post-ruminal digestibilities were not statistically different. But the ruminal digestibilities of hemicellulose (94.81, 90.26 and 85.99) and EG (93.85, 83.30 and 78.77) decreased significantly as the concentrate ratio of the diets increased. The post-ruminal digestibility of hemicellulose (5.19, 9.74 and 14.03%) and GE (6.12, 16.20 and 21.23%) increased as the concentrate ratio of the diets increased.

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This work was carried out at FCAV-UNESP, Campus of Jaboticabal, Brazil, to study the effects of nitrogen fertilization (0 and 50 kg N/ha/cut), three sucessive periods of growth (December 21st 1987, January 25th 1988 and February 29th 1988)) and three cutting ages (28, 35 and 42 days) on composition in crude protein and in vitro dry matter digestibility of two cultivars of Panicum maximum Jacq. i.e., Coloniao and Tobiata. Plants were harvested in five vertical layers above soil level (0-20, 20-40, 40-60, 60-80 and over 80 cm). The samples were divided in: green leaves, green stems and dry material. Generally, values of in vitro dry matter digestibility and the levels of crude protein of both cultivars were greater in the higher layers of the vegetation, with a decrease, however, for the other layers and more advanced phases of plants development, mainly with no N fertilization.

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Corn plants were ensiled at the milk, milk-early dough, medium dough, and semi-hard dough stages. The intake and digestibility trials showed that: a) silage DM and NFE contents increased and ADF, cellulose, and lignin contents decreased with plant maturity; b) corn plant maturity had little effect on the digestibility of the silages, but the milk and medium dough stages produced the best results; c) no consistent effect of maturity was observed in DM and CP daily intakes (mean values 41.86 and 26.77/g/kg(75), respectively). Nevertheless, daily digestible dry matter and digestible protein intakes (g/kg(75)) were higher with medium-dough grains (31.74 and 1.97g, respectively) than with the milk (23.27 and 1.45g, respectively) and the milk-early dough stages (22.60 and 1.28g, respectively). However, similar intakes were observed with the medium-dough and semi-hard dough stages. Total digestible nutrient contents of the silages were affected little by corn plant maturity. Nevertheless, statistical differences were Found between the medium dough (70.09%) and the milk-early dough stages (63.07%).

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To evaluate the nutritional value of palm kernel meal on the performance Nile tilapia (Oreochromis niloticus), five isonitrogenous (30% crude protein), isoenergetic (2800 kcal/kg of digestible energy), and isofibrous (10% crude fiber) diets, with increasing levels of palm kernel meal (0, 7, 14, 21, 28 and 35%). were ad libitum fed to fingerlings with initial body weight of 1.52g +/- 0.04, housed per 120 days in 35 aquarium of 60 liters with fingerlings each. The environmental culture condition were monitored during all experimental period. Statistical analyses of recorded data were performed through polynomial regression models. To determine the apparent digestibility, macroscopic analysis of viscera and microscopic analysis of intestine epithelium were performed. The obtained results indicated that the inclusion of palm kernel meal up to 35% Nile tilapia diets did not affects the apparent digestibility and did not induce any pathological effects on viscera and intestinal epithelium.

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The intake, the apparent digestibility and the nutritive value of elephant grass (Pennisetum purpureum Schum.) silages prepared with the addition of 0, 8, 16 and 24% of ground ear corn with husks, wheat bran and saccharin, dry weight of additive/wet weight of green chop upon the silage were evaluated. A randomized block design with three replications, in a factorial arrangement (3 additives x 4 levels) was used. As experimental silos, 200-liter plastic vessels were used. Sheep weighing approximately 50 kg, kept in individual cages, receiving water and mineral mixture ad libitum, were used to measure the intake and apparent digestibility of silages. There was a ten-day period of adaptation to the experiment conditions. The voluntary intake of the silages was determined by the mean of the intake observed in the last three days of a ten-day period. The fecal collection period lasted for seven days. In this period the animals were fed 80% of the observed intake obtained in the previous phase. The dry matter intake increased as the levels of the additives in the silages were increased. The digestibility of the wall cell components decreased as the rates of the additives in the ensilage process increased. The silages prepared with wheat bran or ground ear corn with husks showed higher nutritive value than the ones with saccharin.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The mechanisms involved in the absorption of amino acids and oligopeptides are reviewed regarding their implications in human feedings. Brush border and basolateral membranes are crossed by amino acids and di-tripeptides by passive (facilitated or simple diffusion) or active (Na + or H + co-transporters) pathways. Active Na +-dependent system occurs mainly at brush border and simple diffusion at basolateral, both membranes have the passive facilitated transport. Free-amino acids use either passive or active transport systems whereas di-tripeptides do mainly active (H + co-transporter). Brush border have distinctive transport system for amino acids and di-tripeptides. The former occurs mainly by active Na + dependency whereas the later is active H +-dependent with little affinity for tetra or higher peptides. Free amino acids are transported at different speed by saturable, competitive carriers with specificity for basic, acidic or neutral amino acids. Di and tripeptides have at least two carriers both electrogenic and H +-dependent. The basolateral membrane transport of amino acids is mostly by facilitated diffusion while for di-tripeptides it is an active anion exchange associated process. The main regulation of amino acids and di-tripeptide transport is the presence o substrate at the mucosal membrane with higher the substrate higher the absorption. Di and tripeptides are more efficiently absorbed than free amino acids which in turns are better absorbed than oligopeptides. So di-tripeptides result in better N-retention and is particularly useful in cases of lower intestinal absorption capacity. The non-absorbed peptides are digested and fermented by colonic bacteria resulting short-chain fatty acids, dicarboxylic acids, phenolic compounds and ammonia. Short-chain fatty acid provides energy for colonocytes and bacteria and the ammonia not fixed by bacteria returns to the liver for ureagenesis.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Two trials were conducted aiming to of evaluate the effect of probiotic supplementation in pig's diet: Trial 1: diarrhea incidence and performance; Trial 2: feed intake and digestibility. In the Trial 1, forty weaning barrow piglets were distributed in four treatments: T0-basal diet; T100-basal diet +100ppm of probiotic; T200-basal diet +200ppm e T300-basal diet +300ppm. The trial 2 was a digestibility trial, where eight barrow pigs were used, distributed in two treatments: T1-basal diet and T2-basal diet + 200 ppm of same probiotic used in the Experiment 1. In the Period 1 the animals of T0 and T100 groups showed higher diarrhea incidence (P<0.05) than the T200 and T300 groups. The performance of animals of T100 group were lower than other treatment groups (P<0.05). In the Period 2 the T200 and T300 animals, showed better FG ratio than the animals of T0 (P<0.05). In the total period it was not observed significative difference concerning performance, except for DFI. In the trial 2, the animals of treatment T2, showed an increase of feed intake when compared with animals of T1. Digestibility coefficients showed no significative differences among treatments. It was concluded that the addition of 200 and 300 ppm of probiotic in the Period 1 reduces incidences of diarrhea. But, in the total period of trial 1, the performance was similar among treatments. In the trial 2 it was observed better adaptation of animals receiving probiotics which was represented by higher feed intake.

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Twelve elephant grass genotypes were evaluated in a randomized blocks design with three repetitions. The experimental parcel was of 4 lines with 3m of length, spaced of 1 m and fertilized with 100 kg of P2O5, 100 kg of N, 60 kg of K2O and 25 kg of micronutrients/ha. Significant differences were observed among the genotypes for neutral detergent fiber (FND), acid detergent fiber (FAD) and lignin. The genotypes CNPGL 92-70-02, CNPGL 91-25-01 and CNPGL 93-32-02 showed low values for FND, FAD and lignin, simultaneously, outstanding between the others. There were no significant differences in the levels of dry matter and crude protein contents and in in vitro dry matter digestibility between the genotypes.