140 resultados para chains with unbounded variable length memory


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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Caseins are the major milk proteins associated with lactation performance, milk composition and cheese yield efficiency, representing around 80% of the total amount of proteins found in milk. Among the caseins, kappa-casein is the protein that stabilizes micelle structure during milk coagulation process and being used during the cheese production. The kappa-casein gene (CSN3) has been previously mapped to buffalo chromosome 7 using a radiation hybrid panel and a comparative map was established using the sequence from bovine chromosome 6. The molecular structure of this gene has also been established in river buffalo, with a total length of 13,191 bp (GenBank: AM900443.1) and containing five exons. In this study we searched for single nucleotide variations in specific regions of the CSN3 gene in three animals representing the Murrah breed. Sequencing reactions were performed using ABI3730xl sequencer. The primer walking method was used to span the 5'-UTR and intron 2 regions of the gene, for which ten primer pairs were designed using Oligo 6 software. BLAST tool was used to verify the primers specificity. DNA sequences assemblies from all three animals were performed with Sequencher (R) software 4.1, while multiple alignments were performed using Clustal W software to identify single nucleotide variations. The sequencing revealed a total of 19 single nucleotide variations with 13 located in the upstream regulatory region of the gene (5'-UTR) and six on intron 2. These variations can be validated using commercial populations segregating specific economic traits.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The genus Corydoras, the highest among the Siluriformes, Callichthyidae is owned by the family, consisting of 177 valid species and widely distributed in cis-Andean portion of South America How striking feature has two longitudinal series of dermal plates covering almost the entire body. Cytogenetic studies in Callichthyidae show many chromosomal rearrangements, including events of polyploidy in their evolutionary history, particularly the genus Corydoras, in which the variation is the diploid number of 2n = 40 to 2n = 134 chromosomes. The absence of information on the frequency of chromosomes in the group Bs motivated this work with the species Corydoras aeneus. A population from the Tietê River basin in Ribeirão Claro (subbasin Corumbataí - Rio Claro, SP) was sampled a total of 20 subjects (10 males and 10 females) and 30 metaphases per individual were analyzed cytogenetically. Were carried out by impregnation techniques silver (Ag-NOR). The observed modal diploid number was 2n = 60 (26m +26 sm +8 st), with the variable occurrence of 1 or 2 Bs chromosomes in males and a B chromosome in females, both acrocentric. Regarding the variation in the frequency of chromosome Bs, the occurrence of two B chromosomes is directly linked to males, because there were no female sampled with the occurrence of two chromosomes Bs The low frequency of Bs in females suggests that this event can be sporadic this sex, different from males in which this appears to be set supernumerary chromosome showing a higher frequency of 2 Bs than the actual modal number of 2n = 60. Further studies will be performed to understand the dynamics of the B chromosome in the population

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Techniques based on signal analysis for leak detection in water supply systems typically use long pressure and/or flow data series of variable length. This paper presents the feature extraction from pressure signals and their application to the identification of changes related to the onset of a leak. Example signals were acquired from an experimental laboratory circuit, and features were extracted from temporal domain and from transformed signals. Statistical analysis of features values and a classification method were applied. It was verified the feasibility of using feature vectors for distinguish data acquired in the absence or presence of a leak.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Pós-graduação em Relações Internacionais (UNESP - UNICAMP - PUC-SP) - FFC

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Writing about philosophical practice with children requires a memory of the body, a body that holds on to what is important to itself. My memory begins with my contact with the ideas of Matthew Lipman and the new ideas brought by his words, and continues with the need to change some of them and assign different meanings to others. Since my reading of the thinkers of the so called “Frankfurt School,” some words have taken new meanings to me, and have informed the way I now understand the practice of philosophy with children and its relationship to issues like educational “formation,” as well as others. Philosophical practice is unique, and needs to be thought, felt, and experienced; it has its own time and involves the construction and transformation of subjectivity itself. As such, to search for words in philosophy means to chose those words that can help us make sense and give meaning of what we do and think, allowing us to work with our thinking and with its forms of expression, beyond its technical dimension. In this sense, the usual emphasis of philosophy in its more technical dimension leads to an impoverishment of formation as experience, for the latter, which is a fundamental dimension of our lives, is rendered secondary. This has implications for the relationship between adults and children. When they reduce philosophy to a study of the formal capacity of thinking, teachers put students in the condition of a minority, and therefore in some way also put themselves in such a condition. In this paper, the activity of writing - as a way of expressing thought - allows me to conduct a tour my own subjectivity, and to encounter the words that express the meanings that inform what I think and do about my practice with philosophical novels, and about the value of generating texts related to philosophical practice, formation and assessment.

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This text presents the Pedagogy of Alternating methodology, highlighting its contribution to re-think the formation of catadores of recycling materials (rubbish collectors). Whereas the use of this approach in the urban area is unusual, the goal of the article is to initiate a debate to promote further elaborations. Initially an overview of the Pedagogy of Alternating is presented and the main instruments used in this approach are commented. Two theoretical and methodological issues are indicated as challenges for catadores formation: the relations between Education and Work and the relations between Theory and Practice. The need to overcome dichotomy between these dimensions of human activity (that predominates in today's society) is defended. The catadores work reveals the exclusion of these workers of less organized sectors of production chains, with few opportunities to obtain better income and qualification. Overcome this gap requires an intense training to turn the catadores in recycling workers . To reach this goal the implementation of Alternating experiences between study and work in cooperatives and schools is not enough. A deep transformation is needed over the ways and subjects of both study and work, in order to create emancipating activities that promotes a full development of these workers.

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The representation of the work of memory and the search for identity in The voyage out by Virginia Woolf In The Voyage Out, Virginia Woolf portrays the protagonist by showing her inner thoughts. Strategy that analogically allows the conception of the esthetic work of the author as being close to the Freudian memory supported by life and death drives. The objective of this study is to show how the psychic states, revealed by the protagonist, are articulated with the work of memory and the search for identity in the novel.

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The aim of this study was to evaluate the influence of specimen size, in comparison with the ISO Standard, on the three point flexural strength of resin composite restorative materials Filtek Supreme and Filtek Z-250. Forty specimens were fabricated for each material with the following length, width and thickness measurements (n = 10): 1) 20 × 2 × 2 mm (ISO 4049); 2) 10 × 2 × 1 mm; 3) 10 × 1 × 1 mm; 4) 8 × 0.8 × 0.8 mm. The composites were inserted in a single increment into two-piece metal device and light-polymerized. The specimens were dry stored at 37 ± 1 °C and protected from light for 7 days. After this period, flexural strength was measured by three-point flexure test using MTS 810 equipment, with a load cell of 10 kN at a speed of 0.5 mm/min. For the evaluated sizes, the results showed significant variability (p = 0.00) with values when compared with the ISO Standard (116.700 MPa), being statistically higher for the test specimens measuring 10 × 1 × 1 mm (142.530 MPa), similar for those of 10  ×  2  ×  1  mm (115.815 MPa) and lower for those of 8 × 0.8 × 0.8 mm (86.650 MPa). There was statistical equality (p = 0.08) for the studied composites (Filtek Supreme, 125.270 MPa; Filtek Z-250, 108.130 MPa). Specimens measuring 10 × 2 × 1 mm provided flexural strength values equivalent to those obtained in the sizes recommended by the ISO 4049 standard, with lower consumption of material, energy and time.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)