146 resultados para Type Iii Restriction-modification System


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The main objective of the presented study is the development of a predictive interval type-2 fuzzy inference system in order to estimate the mortality risk for a newborn, to be used as an auxiliary tool for decision making in medical centers where there is a lack of professionals for this purpose and, afterwards, to compare its performance to a type-1 fuzzy system. The input variables were chosen due to their acquisition ‘simplicity, not involving any invasive tests, such as blood tests or other specific tests. The variables are easily obtained in the first few minutes of life: birth weight, gestational age at delivery, 5-minute Apgar score and previous report of stillbirth. Databases from the DATASUS were used to validate the model. 1351 records from the city of São José dos Campos, a mid-sized city in the São Paulo state’s countryside, were considered in this study. Finally, an analysis using the ROC curve was performed to estimate the model’s accuracy

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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Pós-graduação em Engenharia Elétrica - FEIS

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Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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ObjectiveTo compare the sequential healing at immediately loaded implants installed in a healed alveolar bony ridge or immediately after tooth extraction.Material and methodsIn the mandible of 12 dogs, the second premolars were extracted. After 3months, the mesial roots of the third premolars were endodontically treated and the distal roots extracted. Implants were placed immediately into the extraction sockets (test) and in the second premolar region (control). Crowns were applied at the second and third maxillary premolars, and healing abutments of appropriate length were applied at both implants placed in the mandible and adapted to allow occlusal contacts with the crowns in the maxilla. The time of surgery and time of sacrifices were planned in such a way to obtain biopsies representing the healing after 1 and 2weeks and 1 and 3months. Ground sections were prepared for histological analyses.ResultsAt the control sites, a resorption of the buccal bone of 1mm was found after 1week and remained stable thereafter. At the test sites, the resorption was 0.4mm at 1-week period and further loss was observed after 1month. The height of the peri-implant soft tissue was 3.8mm both at test and control sites. Higher values of mineralized bone-to-implant contact and bone density were seen at the controls compared with the test sites. The differences, however, were not statistically significant.ConclusionsDifferent patterns of sequential early healing were found at implants installed in healed alveolar bone or in alveolar sockets immediately after tooth extractions. However, three months after implant installation, no statistically significant differences were found for the hard- and soft-tissue dimensions.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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The dental trade has offered dental impression and dental stone for orthodontic use ensuring accurate models. The compatibility between these materials is defined by the wetting of the model surfaces by the mixture water/stone poured over it and the influenced by the method of disinfection of model and disinfectant solution used. It was evaluated the influence of spray disinfection with sodium hypochlorite 1% on the wettability of two commercial alginate (Jeltrate ® - Dentsply and Orthoprint ® - Zhermack) at two commercial type III gypsum (Rio ® - ME and AOBussoli Orthogesso Orthogesso ®-SA). Twenty models were fabricated for each type of alginate, which were divided into two groups (water and sodium hypochlorite), receiving respectively water and sodium hypochlorite 1% spray. Each group of models was then further divided into two subgroups, and on their surface were poured 2 ml of type III gypsum (Gesso Rio® or Orthogesso®). Reached the final setting of the gypsum specimens were sectioned vertically and medially, settled water with sandpaper No. 400 and mounted on suitable device for reading (in the right and left) of the contact angle Carl Zeiss microscope (precision, 001). The results were submitted to ANOVA and founded statistical significance for solutions used. It was concluded that sodium hypochlorite spray improved wettability of alginates studied.

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The cares at the manipulation of the alginate (condensation, disinfection of the impression and the time elapsed until the leak of the plaster) deserve special attention considering the great amount of distortions occurring every time the impression is not fill of plaster in a brief space of time and appropriate storage conditions. Besides its adaptation to the plaster suffers influence of the events happened after the impression is removed of the mouth. The purpose of this research was to evaluate the wetting capacity of three brands of alginate (Jeltrate™,Hydrogum™ e Orthoprint™) by the plaster type III (Rio™) under the influence of disinfection by sodium hypochlorite 1% sprays and the time of storage of 15 minutes,30 minutes, 1 hour, 6, 12 and 24 hours. There were made 60 impressions of each brand of alginate divided in two groups (water and sodium hypochlorite 1%) rearranged after the application of the sodium hypochlorite spray according to the storage time (15 or 30 minutes and 1,6,12, or 24 hours). On the surface of the impressions 2ml of plaster were flowed, proportioned and condensed in agreement with manufacturer‘s instructions. After the final setting expansion the casts were sectioned vertically and medially, regularized at the cut surface (emery paper 400) and setted for reading the contact angle at the microscope Carl Zeiss. The obtained results, submitted to statistical treatment (ANOVA) revealed significant differences when compared the employed solutions (water and sodium hypochlorite 1%) and the time of storage. The sodium hypochlorite 1% exhibited the smallest contact angles and the times of storage of 15 minutes and 6 hours the smallest and larger angles, respectively. It can be concluded that the alginate impressions exhibited larger adaptation to the plaster when disinfected by hypochlorite of sodium 1% and stocked by 15 minutes.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The present work realized a comparative study in meiosis of two triatomines, Panstrongylus herreri and P. megistus, by cytogenetic techniques involving the restriction endonucleases Hae III and Alu I and C-banding. The system of sex chromosomes in Panstrongylus is of the X1X2Y type, and experiments corroborated the common origin hypothesis of the X chromosomes by fragmentation of single X. In both species the restriction endonucleases (RE) presented banding patterns in part similar to C-banding. However, in some early meiotic phases it was possible to verify differentiation of the heterochromatic pattern. This work suggests that other elements besides presence of recognition sites, such as chromatin packing degree and DNA-protein interaction, act in RE results, since digestion patterns occur in early spermatogenesis. However, metaphase chromosomes were practically inaccessible to the endonucleases.