Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes

This work was carried out with Psychotria ipecacuanha, a Brazilian medicinal plant the roots of which contain emetine. The main objective was to develop a protocol for the micro-propagation of these species, by testing different culture techniques, the temporary immersion system, and the semi-solid and liquid media systems. In the semi-solid system, experiments were developed in flasks of two different sizes containing MS, B5, and WP media to which were added different growth regulators. Innoculum density was also evaluated. The liquid medium system consisted of MS medium supplemented with different growth regulators. For the temporary immersion system, the MS medium received an addition of 1.5mg/L BAP and 0.5mg/L GA3, and a reverse digital apparatus and vacuum pump were used. The liquid medium system with MS medium supplemented with 1.5mg/L BAP and 0.5mg/L GA3 presented the best results for shoot proliferation in a period of 30 days in culture (2.37 ± 0.32 shoots/explant). Cultures carried out for 90 days in the semi-solid system, using 8.5 × 5.5cm flasks and 3 explants per flask, developed 1.80 ± 0.20 shoots/explant, achieving 3.06 ± 0.51 cm of height adn presented superior survival ratio (96%). Explants cultured in temporary immersion system for 90 days showed 2.30 ± 1.10 shoots/explant achieving a growth of 2.08 ± 0.12 cm and 52% survival.

Uso e conservação de Piper cernuum Vell. (Piperaceae) na Mata Atlântica: I. Fenologia reprodutiva e dispersão de sementes

The pariparoba (Piper cernuum Vell.), a native plant from the Atlantic Tropical Forest (ATF), has been commonly used in folk medicine. Works seeking the exploitation of P. cernuum natural populations need to be accomplished, seeking compatibility in obtaining income rates and preservation of ATF. The present work aimed to study the reproductive phenology and seed dispersal in P. cernuum natural population in the counties of Sete Barras/SP (Parque Estadual Intervales - PEI). The population of P. cernuum studied in PEI presented continuously bud-inflorescences, green-infrutescences and bud-inflorescence emission, with variation among individuals. Fruit-set average was of 9.1%, varying among individuals. Shading and foliar damage presented negative effect on the fruit-set. Three species of bats were identified as potential dispersors of the P. cernuum seeds: Carollia perspicillata, Anoura geoffroyi and Artibeus sp. The foragement strategy observed was the withdrawal of some seeds per infrutescence per flight. This strategy increases the potential in establishing new plants as well as in increase the potential of gene flow in comparison with the entire infrutescence plant eaten at the night perches. In consequence, the results of reproductive phenology and seed dispersal ecology presented in this work can help to establish management and conservation strategies of P. cernuum natural populations in the ATF.

Produção de composto orgânico a partir de residuos de plantas medicinais

Extraction of active principles from medicinal plants generates wastes usually discharged. This process does not extract minerals from plant tissues, which may be considered of great value in organic composting. Wastes of 81 medicinal plant species processed at Centroflora/Anidro industry were analyzed for macro and micronutrients, and according to the results, three different ways to elaborate the compost were proposed: a) wastes of medicinal plants with high content of a specific mineral, aiming to fertilize soils where would be cultivated species that need high quantities of that mineral; b) wastes with mineral contents in order to support specific failures on mineral content of a soil; c) wastes of the eleven richest species in each nutrient that should elevate all mineral contents in soil.

Caracterização histológica de folhas de Mentha pulegium x spicata (Lamiaceae)

The glandular hairs of Mentha pulegium x spicata (Lamiaceae) were studied by light microscopy and scanning electron microscopy, Three morphologically distinct types of trichomes are described: two of them are glandulars (peltate and capitate) and one is non-glandular hair (tector). Capitate trichome is of Type I, and consists of a foot cell, a stalk and a unicellular head with elongated format and two lateral depressions, showing the thinnest cuticle. The peltate trichome consists of a foot cell, a stalk cell and a radial cluster of secretory cells, cover by cuticle. The tector trichomes can be simple and forked -format, both multicellular, in which there are ornamentations on cell cuticle. The leaf cross sections, analysed in light microscopy, showed mesophyll organized for unistratification palisade parenchyma and lacurary parenchyma with four to five cell stratum. It was observed the presence of cristal mass agglomerate in mesophyll cells and in epidermical cells.

Productivity of beet and roquette cultivation as a function of time of establishing intercropping

With the aim of evaluating the effect of intercropping on the productivity of beet and roquette crops, a study was conducted in the Section of Vegetable and Aromatic-Medicinal Plants, FCAV-UNESP, Jaboticabal/SP. The treatments consisted of co-cultivations of roquette (Cultivada) and beets (Early Wonder) established at 0, 7, 14 and 21 days after the transplanting of beets, and as monocultures of beets and roquette. The experiments were delineated in a randomized block design. The productivity of beets in monoculture was not superior to that obtained with intercropping with roquette. The production of dry mass of roquette with intercropping was less than that with monoculture and the reduction was more marked with more delayed establishment of co-cultivation. Intercropping established at 0 and 7 days after the transplanting of beets was the most efficient, with indices of efficient use of land of 1.26 and 1.27, respectively.

Total and harvest-date yield of processing tomato cultivars

The productivity of 28 tomato cultivars was evaluated over three stages of harvest. The study was carried out during from June to December of 1999 in an open field at the experimental area of the Section of Olericulture and Aromatic Medicinal Plants, Department of Crop Production at FCAV-UNESP, Jaboticabal, SP, Brazil. The cultivars studied were H 7155, Hypeel, Andino, U 573, H 9036, IPA 6, H 9494, AG 33, Yuba, RPT 1294, AG 72, Pelmeech, Curico, Hypeel 45, RPT 1478, H 9492, H 9498, H 2710, Hitech 45, Halley, Botu 13, H 9553, U 646, NK 1570, AG 45, RPT 1095, RPT 1570, and PSX 37511. The experimental design was a randomized block design with four repetitions, with five plants per plot. Productivity was evaluated at three stages of harvest at 119, 149 and 179 days after seeding. There were no significant differences among the cultivars at the first harvest (119 days). The majority of the cultivars produced their highest yield at the second harvest; the most productive cultivars were Curicó and AG 72, which yielded 4.69 and 4.67 kg/plant, respectively, although they did not differ statistically from the cultivars Hypeel 45 (4.35 kg/plant) and H 9498 (4.16 kg/plant). Yields of the cultivars Andino and H 9494 were evenly distributed between the second and third harvests. At the third harvest, cultivar IPA 6 had the highest yield (2.9 kg/plant) and was statistically different from all other cultivars except H 9036 (2.34 kg). These two cultivars had the most delayed and concentrated maturity, making them suitable for mechanical harvesting, although at a later time. Cultivar AG 72 had the greatest total yield (5.76 kg/plant), but it was not statistically different from cultivars Hypeel 45 (5.43 kg), Curico (4.17 kg), H 9498 (4.83 kg), H 7155 (4.58 kg) and Halley (4.55 kg). All of the cultivars, with the exception of cultivars H 9036, IPA 6, Andino and H 9494 showed in the second harvest concentrated maturity, making it suitable for mechanical harvesting.

Das plantas medicinais aos fitofármacos

The plants, since the antiquity, have been a resource within reach of the human being. Along millenniums, the man empirically deepened its knowledge for the improvement in the alimentation and cure conditions of its illnesses, demonstrating a narrow relation between plant use and its own evolution. A lot of people described the herbs utilization like medication form in their records and manuscripts, but many centuries have been passed until the true power of the plants was recognized. The great discoveries of the active plants principles only were possible after technological advances for the isolation and structural elucidation. Several active substances with pharmacological activity, many times indicated by the popular use, were going proved scientifically. Nowadays, the man still seeks solutions for several diseases and health problems and maybe the plants can contribute of more significant way for resolve them.

Essential oil composition of Aloysia triphylla (L'Herit) Britton leaves cultivated in Botucatu, São Paulo, Brazil

The family Verbenaceae comprises about 175 genera and 2300 species, distributed in tropics and subtropics, mainly in temperate zone of southern hemisphere. The lemon verbena (Aloysia triphylla (L'Herit) Britton) is a perennial, bushy plant originally from South America. The essential oil of this plant is used in pharmaceutical, cosmetic and perfumery industry. Therapeutic properties include febrifuge, sedative, stomachical, diuretic, and antispasmodic activities. The present work aimed to identify the chemical composition of essential oil of Aloysia triphylla leaves. The study was done in Lageado Experimental Farm of the Department of Plant Production-Horticulture, Agronomical Sciences College, São Paulo State University Campus of Botucatu. Leaves of lemon verbena from Medicinal and Aromatic Plant Garden, were collected in the end of winter (September/2001). The essential oil was extracted by hydrodistillation, in Clevenger apparatus. 100 g of leaves were used in each extraction. Four extractions were performed during three hours. The essential oils of the leaves were analyzed in Gas Chromatography Mass spectrometry (CG-MS, Shimadzu, QP-5000), equipped with capillary column DB-5 (30 m × 0,25 mm × 0,25 mm), split 1/35, injector for 220 C°, detector for 230 C°, dragged by gas He (1,0 mL/min), with programmed temperature for 60 C° to 240 C°, 3 C°/min. The identification of the substances was held by comparison of their mass spectra with data of the CG-MS (Nist 62 lib), literature references and retention index of Kovats. The main constituents of essential oils were geranial (29.54 %), neral (27.01 %), limonene (15.93 %), geranyl acetate (4.0 %) and geraniol (3.96 %). This species possesses high quantity of monoterpenes and low quantity of sesquiterpenes.

Influence of IBA and boric acid on rooting of stem-cuttings of Aloysia triphylla (L'Hérit) Britton

Aloysia triphylla (L'Hérit) Britton is a perennial and bushy plant, with simple, entire, lanceolate and whorl shaped leaves and originally from South America. It is used as medicinal plant in Brazil with stomatic and sedative properties. The employment of stem cuttings for propagation of pre-selected plants, acquired great importance, because it eliminates the juvenile phase of seedlings, which can be produced in a shorter period of time. The rooting of stem cuttings is stimulated by auxin and, boric acid supply is essential for growth and development of initial rootlets. This micronutrient is required 48 hours after plant segments have been placed into auxin solution and it can be supplied any time, including the seedling growth period. The experiment was carried out in the Department of Plant Production, UNESP-Botucatu-SP-Brazil, with stem-cuttings of Aloysia triphylla (L'Hérit) Britton, Verbenaceae obtained from the Medicinal and Aromatic Plant Garden. The aim of the work was to verify the influence of growth regulators and boric acid on stem cutting rooting of this species. The 15 cm-stem cuttings, without leaves, were submerged during 24 hs in the following solutions: water; 150 mg.L-1 of IBA; 150 mg.L-1 of IBA+ Boric acid; 250 mg.L-1 of IBA; 250 mg.L-1 of IBA + Boric acid. The statistical design was entirely randomized with 5 treatments and 3 replications, totalizing 15 plots with 10 stem cuttings each. They were planted on propylene trays with vermiculite and kept under spraying condition during twenty five days. The best results were observed in treatment 250 mg.L -1 of IBA+ Boric acid on number of roots, length of roots, rooting percentage, fresh and dry weight of leaves when compared with all other treatments. We can conclude that this treatment is the most suitable for stem cutting rooting of this species.

Efeitos hipotensivo e diurético dos extratos aquosos de pitanga (Eugenia uniflora L.) e jambolão (Eugenia jambolana Lam.) em ratos normotensos anestesiados

Plants that possess a diuretic effect are widely used by people in the treatment of some important diseases as edema and hypertension. The objective of this work was to study the effects of pitanga and jambos aqueous extracts (AE) about the arterial pressure (AP) and urinary flow (V) in normotensive and anesthetized rats. The AE were prepared for the decoction method and administrated for intragastric way in different concentrations: 10%, 15%, 20% and 25%. These concentrations corresponded respectively at doses of 56, 94, 145, 172 mg of pitanga dried extract /Kg and 44, 73, 83, 95 mg of jambos dried extract/Kg. The animals were divided in nine groups with seven individuals (n=7): control (C), P-10%, P-15%, P-20%, P-25%, J-10%, J-15%, J-20% and J-25%. The rats were anesthetized (hypnol 3%) and submitted to tracheotomy. The left carotide artery was catheterized to measure the AP through a mercury manometer, in periods of 15 minutes. The bladder was catheterized for urine collection and to measure the V, in periods of 30 minutes. The experimental protocol was divided in four periods of 30 minutes each: basal (to evaluate of the basal parameters) and experimental (Exp) 1, 2 and 3 (after the administration of the AE). The results were analyzed for ANOVA and Tukey (X±SD, p<0.05). In the C group did have not alteration of the AP basal but the V basal increased. In the experimental groups (AE of P and J) had significative decline in the AP basal: 34% (P-10%), 20% (P-15%), 21% (P-20%), 31% (P-25%), 24% (J-10%), 20% (J-15%) 16% (J-20%) and 29% (J-25%). Moreover, the administration of AE increased the V basal in: 280% (P-15%) and 192% (J-20%). The results showed that the plants evaluated are hypotensive and diuretic.

Aplicação exógena de artemisinina e indução floral em Artemisia annua L.

The objective of this work was to evaluate the effect of different artemisinin concentrations in the flowering induction of A. annua. Two genotypes of A. annua (CPQBA 239x1V and 3MxPOP) were atomized with four different artemisinin concentrations (0, 500, 5000, and 10000 mg L-1). The application of artemisinin didn't induce the flowering of both genotypes tested, in none of the used concentrations.

Bioatividade de plantas medicinais no controle de Sclerotinia isolado de Stevia rebaudiana (Bert.) Bertoni

The microorganism Sclerotinia was isolated from roots of Stevia rebaudiana (Bert.) Bertoni in plantations in the northwest of Parana and submitted to the cultivation in the presence of extracts and vegetable balsams of Tarragon (Artemisia draconculus), Thyme (Thymus vulgaris), Manjerona (Origanum majorona), Mint citrata (Mintpiperita var. citrata), Purple Basil (Ocimum basilicum L.), Andiroba (Carapa guanensis) and Copaíba (Copaifera reticulata Ducke). The first five oils were extracted by steam drags, after the drying of the vegetable in greenhouse with circulation of air at 45°C. The last two were used in natura. A suspension (100ìl) of fungus previously cultivated, was added to each plate. The results show that after 7 days of incubation the thyme oils 10ìl, purple basil 25ìl, manjerona 25ìl, mint citrata 50ìl, tarragon 50ìl were capable to inhibit the growth of Sclerotinia, while the andiroba oil only reached this result with 200ìl. The copaiba balsam, even in the concentration of 500ìl, was unable to inhibit the growth of the microorganism.

Desenvolvimento e produtividade da cúrcuma (Curcuma longa L.) em função de doses de nitrogênio e potássio

The objective of the experiment was evaluating the development and production of turmeric, it was carried out at FCAV-UNESP, in Jaboticabal-SP, during 11/18/ 1999 to 7/18/2000, in randomized blocks, with three replications, factorial scheme 5 × 5. The factors evaluated were doses of N (0, 68, 136, 170 and 204 kg.ha-1) and doses of K2O (0, 92, 184, 230 and 276 kg.ha-1). The potassium doses influenced plant-mother's height significantly, answering lineally to the increment in the potassium fertilization. There was significant interaction between nitrogen and potassium on the number of leaves of the sons and total-plant. In the doses of 0 and 92 kg of K2O.ha-1 a lineal increase was observed in the number of leaves with the increment of the applied nitrogen. The potassium doses also influenced the foliar area of the sons and total-plant, reaching values of 3260 and 4971 cm2 per plant, respectively, for the application of 276 kg K2O.ha1. The number of sons had a lineal increment with the growing fertilization of potassium, contemplating on the dry mass of the aerial part of the sons. The production of rhizomes per plant wasn't influenced for the factors evaluated.

Efeitos do tipo de rizoma de multiplicação e da cobertura morta no desenvolvimento e produtividade de cúrcuma (Curcuma longa L.)

The aim of this study was to evaluate the influence of different types of rhizomes utilized as seed and the effects of mulch in the culture of Turmeric. This work was conducted in the experimental cultivation area of the Universidade de Ribeirão Preto - UNAERP, in Ribeirao Preto, state of Sao Paulo, Brazil from September/1997 to July/1998. The experiments were arranged in a randomized complete block with a factorial arrangement 2x3, design with 4 replications (32 plants each). The treatments consisted of the use of types of rhizomes utilized to proliferation (head and finger) and types of mulch (absent, grass and leaves/branches). This study investigated proliferation (61, 88 and 124 days after installation), length, shooting and number of leaves of the mother plant besides the number of leaves of the shoot (112, 146 and 233 days after installation), number of finger rhizomes and head rhizomes, fresh and dry biomass of rhizomes and the yield of essential oil (after harvest - July 1998). Obtained results showed a great influence of the type of rhizome used to proliferation. Head rhizomes produced, in weight, about 30% more, when compared to plants from finger rhizomes. Mulching almost duplicated rhizome productivity from 2338 kg.ha -1 of dry rhizomes to 4499 kg.ha -1, when rhizome used to proliferation was finger and from 3046 kg.ha -1 to 5943 kg.ha -1, when using head rhizomes. The yield of essential oil did not show any alteration, independently of the treatments realized.

Composição do óleo essencial de rubim (Leonurus sibiricus L. - Lamiaceae)

The essential oils are found in a great number of Lamiaceae family species, but few researches were carried out on this subject. This work had as a goal to identify the Leonurus sibiricus L. (rubim) essential oil composition. Rubim leaves and flowers in infusion are able to avoid vomit, diarrhea, and are also indicated in cold, cough, bronchitis and rheumatisms cases. In order to know what are the phytochemical compositions involved, it was used the gas chromatography techniques with mass spectrometry (GC-MS) as methodology. The results showed 70% volatility compound by trans-cariophylene, alpha-humulene and germacrene-D. Other substances like gamacadinene, beta-bourborene and alpha-copaene were found like compounds of this essential oil species.