A preliminary investigation of pre-dispersal seed predation by Acanthoscelides schrankiae horn (Coleoptera: Bruchidae) in Mimosa bimucronata (DC.) Kuntze trees

This is the first record of Acanthoscelides schrankiae Horn, feeding in seeds of Mimosa bimucronata (DC.) Kuntze. We investigated the pattern of oviposition and seed exploitation by A. schrankiae, and the distribution of mature fruits and seed predation in the inflorescences. We also compared the percentage of predated seeds, the total dry weight of fruits and non-predated seeds, the percentage of aborted seeds, and the percentage of non-emergent insects, among different quadrants of the M. bimucronata canopy. To determine the occurring species, the emergence of bruchids and parasitoids was observed in the laboratory, resulting altogether, only in individuals of A. schrankiae and Horismenus sp. (Hymenoptera: Eulophidae) species, respectively. Mean number of fruits produced in the median region of inflorescence was significantly higher than in the inferior and superior regions, and the frequencies (observed and expected) of predated and non-predated seeds differed among the different regions of inflorescence. Females of A. schrankiae laid their eggs on fruits, and larvae, after emergence, perforated the exocarp to reach the seeds. Most fruits presented one to three eggs and only one bruchid larva was observed in each seed. The highest value of the rate number of eggs/fruit and the highest percentage of predated seeds were recorded in April. Dry weight of fruits (total) and seeds (non-predated), proportions of predated seeds, seed abortions, and non-emergent seed predators, were evenly distributed in the canopy.

Few substitutions affect the bioluminescence spectra of Phrixotrix (Coleoptera: Phengodidae) luciferases: a site-directed mutagenesis survey

Phrixotrix (railroad worm) luciferases produce bioluminescence in the green and red regions of the spectrum, depending on the location of the lanterns, and are the only luciferases naturally producing red bioluminescence. Comparison of the luciferase sequences showed a set of substitutions that could be involved in bioluminescence colour determination: (a) unique substitutions in the red luciferase replacing otherwise invariant residues; (b) conserved basic residues in the green-yellow emitting luciferases; and (c) an additional R353 residue in red-emitting luciferase (Viviani et al., 1999). To investigate whether these sites have a functional role in bioluminescence colour determination, we performed a site-directed mutagenesis. Natural substitutions in the region 220-344 and residues in the putative luciferin-binding site were also investigated. With the exception of the previously identified substitution of R215 and T226 (Viviani et al., 2002), which display dramatic red-shift effects on the spectrum of green-yellow-emitting luciferases, only a few substitutions had a moderate effect on the spectrum of the green-emitting luciferase. In contrast, no single substitution affected the spectrum of the red-emitting luciferase. The results suggest that the identity of the active site residues is not so critical for determining red bioluminescence in PxRE luciferase. Rather, the conformation assumed during the emitting step could be critical to set up proper interactions with excited oxyluciferin. Copyright ©2007 John Wiley & Sons, Ltd.

Avaliação da importância da unidade de conservação na preservação da diversidade de Chironomidae (Insecta: Diptera) no córrego Vargem Limpa, Bauru, Estado de Sáo Paulo

This study evaluated the water quality of the Vargem Limpa stream and tested the hypothesis that protected stretches of the aquatic system, located within a conservation unit, favor the preservation of the diversity of Chironomidae (Insecta: Diptera). Samplings were conducted in four stations, where some physical and chemical variables were measured. The results obtained by the study indicated the predominance of genera associated with arenaceous substrata, and that the stretches protected by the conservation unit presented better environmental conditions, with better-preserved Chironomidae diversity, demonstrating the importance of these areas in maintaining the diversity of Chironomidae.

Chromosomal similarities and differences among four Neotropical Elateridae (Conoderini and Pyrophorini) and other related species, with comments on the NOR patterns in Coleoptera

This work deals with the comparative cytogenetic analysis of four Neotropical Elateridae species and reviews the nucleolar organizer region (NOR) patterns on Coleoptera chromosomes, for the first time. The cytogenetic characterization of Conoderus malleatus (Conoderini), Pyrearinus candelarius, Pyrophorus divergens and Pyrophorus punctatissimus (Pyrophorini) was accomplished through the study of mitotic and meiotic cells submitted to standard (Giemsa) and differential staining [silver impregnation and GC-specific chromomycin A 3 (CMA 3) plus AT-specific 4′-6-diamidino-2-phenylindole (DAPI) fluorochromes]. The analysis of spermatogonial cells revealed the diploid numbers: 2n = 17 in C. malleatus and 2n = 15 in P. candelarius, P. divergens and P. punctatissimus. In these species, the X0 type sex-determination system and the acrocentric morphology of almost all chromosomes were observed. The study of meiotic cells of the four species revealed the occurrence of total synapsis between the autosomes, the presence of one terminal or interstitial chiasma in the majority of the bivalents, and the reductional behaviour and regular segregation of all chromosomes. Although the three Pyrophorini species demonstrated many similar karyotypical characteristics, there was one discrepancy, which was noted in the diplotene cells and concerns the number of bivalents with two chiasmata; P. candelarius only presented one bivalent, P. divergens showed two bivalents and P. punctatissimus exhibited up to four bivalents with two chiasmata. Testicular cells impregnated with silver nitrate demonstrated two terminal NORs located on the fourth autosomal pair of the Conoderini species and on the second autosomal pair of the three Pyrophorini representatives. Use of CMA 3/distamycin A (DA)/DAPI staining on the P. candelarius and P. punctatissimus chromosomes revealed that the CMA 3 labelled regions were coincident with the NORs. The main strategies of karyotypical differentiation that have occurred among the four Elateridae species and other related species, and the general trends of the NOR shifts during Coleoptera chromosomal evolution are discussed in this work. © 2007 The Authors.

Mitochondrial genome sequence of the Brazilian luminescent click beetle Pyrophorus divergens (Coleoptera: Elateridae): Mitochondrial genes utility to investigate the evolutionary history of Coleoptera and its bioluminescence

The Coleoptera order is the richest group among Metazoa, but its phylogenetics remains incompletely understood. Among Coleoptera, bioluminescence is found within the Elateroidea, but the evolution of this character remains a mystery. Mitochondrial DNA has been used extensively to reconstruct phylogenetic relationships, however, the evolution of a single gene does not always correspond to the species evolutionary history and the molecular marker choice is a key step in this type of analysis. To create a solid basis to better understand the evolutionary history of Coleoptera and its bioluminescence, we sequenced and comparatively analyzed the mitochondrial genome of the Brazilian luminescent click beetle Pyrophorus divergens (Coleoptera: Elateridae). © 2007 Elsevier B.V. All rights reserved.

Functional feeding habits of Chironomidae larvae (Insecta, Diptera) in a lotic system from Midwestern region of São Paulo State, Brazil

Functional feeding habits of Chironomidae larvae (Insecta, Diptera) in a lotic system from Mid-western region of São Paulo State, Brazil were analyzed. Collectors were the dominant organisms, repre-sented by genera Chironomus, Fissimentum and Cryptochironomus, following by shredders and predators.

Controle de Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) em instalações para frangos de corte

Searching for a new alternative to A. diaperinus control, it was evaluated the action of Spinosad in two concentrations (250ppm, 400ppm) and two doses (0.05 L/m 2 and 0.1 L/m 2), applied in poultry broiler facilities naturally infested by this coleoptera. Assessments of the infestation were held in weekly intervals, during 49 days after treatment, using traps. The percentage of effectiveness were calculated from the results of the number of adults and/or larval stages in control and treated groups. Spinosad at the concentration of 250ppm, applied at a dose of 0.1L/m2, can be considered ineffective against these beetles, however the application of 400ppm at a dose of 0.1L/m2 showed high efficacy and short residual period. The dose of 0.1L/m 22 of Spinosad at the concentration of 400ppm demonstrated, between treatments, better effectiveness against coleopters, reaching efficacy of 100% against larvae of A. diaperinus, observed after the seventh day post-treatment.

Índice de infestação e fatores de mortalidade do bicudo-do-algodoeiro (Coleoptera: Curculionidae) no semi-árido do sudoeste da Bahia, Brasil

The boll weevil (Anthonomus grandis Boheman, 1843) (Coleoptera: Curculionidae) is a pest that limits the production of cotton, especially in the North East of Brazil. The present study aimed to estimate the infestation index and natural mortality of boll weevil at Caraibas County, located in the semi-arid of the Southwest ofBahia. The experiment was performed using the cultivar BSR Aroeira, at Fazenda Lagoa Seca, in 2006. Weekly evaluations were performed, in five sampling areas 25m2, collecting blossoms fallen on the ground. The infestation was estimated by counting the oviposition punctures and /or the feeding on blossoms, and also by the presence of one of the phases of the boll weevil in the inside. Mortality factors were studied through the dissection of blossoms, estimating predation, parasitism, dissection and disease. The results obtained allowed to conclude that the boll weevil determines high infestation levels in the region. Parasitism, dissection and predation, following this order, determine high rates of real mortality of the pest. Catolaccus grandis Burks, 1954 (Hymenoptera: Pteromalidae) and Bracon sp.(Hymenoptera: Braconidae) are parasitoids of boll weevil reported in sothwestern Bahia, being the first one the predominant species. © 2009, Sociedad Venezolana de Entomología.

Predatory behavior of Canthon virens (Coleoptera: Scarabaeidae): A predator of leafcutter ants

We present a detailed description of the predatory behavior of the beetle Canthon virens Mannerheim, 1829, on the leafcutter ant Atta sp. We observed 51 acts of predation, which were also recorded on film and subjected to behavioral analysis. Canthon virens exhibited 28 behaviors while predating upon Atta sp. queens. Adult beetles search for queens while flying in a zigzag pattern, 15 to 20cm above the ground. After catching a queen, the predator stands on its back and starts cutting the queen cervix. Once the prey is decapitated, the predator rolls it until an insurmountable obstacle is reached. The distance from the site of predation to the obstacle can vary widely and is unpredictable. The beetle rolling the queen also buries it in a very peculiar way: first, it digs a small hole and pulls the queen inside, while another beetle is attached to the prey. The burial process takes many hours (up to 12) and may depend on the hardness of the soil and the presence of obstacles. In general, one or two beetles are found in a chamber with the queen after it is buried. They make the brood balls, which serve as food for the offspring. This study contributes to the knowledge of the predatory behavior of Canthon virens, a predator poorly studied in Brazil and widespread in the country. Copyright © 2012 Luiz Carlos Forti et al.

Nitidulidae (Coleoptera) Associados A Frutos de Café (Coffea Arabica L.)

This study reports sap beetles from fruits of a coffee crop in Cravinhos, SP, Brazil. Fruits were collected directly from plants and, in laboratory, from the fruits at the cherry state we obtained 20 adults of three species: Carpophilus nepos Murray, 1864, Colopterus niger Murray, 1864 and Nitops sordidus Erichson, 1847. This is the first report of association between these insects and coffee fruits.

Seleção de isolados de beauveria bassiana (Bals.) Vuill. para o controle de sitophilus zeamais (Mots.) (Coleoptera: Curculionidae)

Sitophilus zeamais (Mots.) (Coleoptera: Curculionidae) is considered a major pest of maize, responsible for reducing grain quality and making the corn inappropriate for industrial use and human consumption. S. zeamais has been controlled exclusively with chemical products. The objective of this research was to select isolates of Beauveria bassiana (Bals.) Vuill. to control S. zeamais. Beetles were immersed in conidia suspensions of each isolate for five seconds and placed in a gerbox container with maize grains. In pathogenicity tests, the isolates that caused the highest mortality to the maize weevil were ESALQ-447 (68.0%), CCA-UFES/ Bb-36 (57.3%) and CCA-UFES/Bb-31 (51.3%). ESALQ-447 was the most virulent, with an LC50 of 1.7 × 107 conidia/ml and shows promise for controlling maize weevils. These isolates of B. bassiana can be used as effective substitutes for conventional chemical control, normally carried out with phosphine. Further tests should be performed under field and semi-field conditions to develop an appropriate strategy for the use of this entomopathogen to manage S. zeamais.

First record of leucospis signifera bouček, 1974 (Insecta: Hymenoptera: Leucospidae) in the São Paulo State, Brazil

The present note extends the geographic range of Leucospis signifera Bouček, 1974 farther to the Northeast, reaching the State of São Paulo, based on the examination of four females collected at Jataí Ecological Station, municipality of Luiz Antônio, São Paulo State, Brazil. The present finding indicates that L. signifera is associated with dry ecosystems, at least in part of its geographic distribution. © 2012 Check List and Authors.

A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis)

The invasive fire ant Solenopsis invicta is medically important because its venom is highly potent. However, almost nothing is known about fire ant venom proteins because obtaining even milligram-amounts of these proteins has been prohibitively challenging. We present a simple and fast method of obtaining whole venom compounds from large quantities of fire ants. For this, we separate the ants are from the nest soil, immerse them in dual-phase mixture of apolar organic solvent and water, and evaporate each solvent phase in separate. The remaining extract from the aqueous phase is largely made up of ant venom proteins. We confirmed this by using 2D gel electrophoresis while also demonstrating that our new approach yields the same proteins obtained by other authors using less efficient traditional methods. © 2013 Elsevier Ltd.

Evolução cromossômica em Cassidinae sensu lato (Coleoptera, Polyphaga, Chrysomelidae)

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Efeito da predação por Acanthoscelides schrankiae(Coleoptera: Bruchidae) e de altas temperaturas na germinação de sementes de Mimosa bimucronata (DC.) Kuntze (Fabaceae: Mimosoideae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)