Efeitos da Isradipina (LOMIR). Agente inibidor de canais de cálcio, no processo de reparo em feridas de extração dental: estudo histológico em ratos

In order to make a histological evaluation of the dental alveolus wound healing in presence of the Isradipine (Lomir/Sandoz Pharma), the new calcium channels blockers, forthy-eight albino rats were utilized. These animals were divided in two groups. The animals of group I were considered as control; in group II, after the dental extractions the rats received intraperitonial injections of 2,5 mg/Kg of isradipine, administered a single dose. From each group of animals were sacrificed at 3, 7, 14 and 21 post-operative days. The pieces were fixed, descalcified and embedded in paraffin. The blocks thus obtained were cut at 6 micrometers thick and sections were stained with hematoxylin and eosin for morphological study. According to the results obtained i t was verified that the treated animals did not alter of wound healing after dental extractions

Efeitos do tipo de sistema adesivo e região do dente sobre a resistência adesiva à dentina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação in vitro da resistência estrutural em dentes fragilizados utilizando reforços intra-radiculares

Pós-graduação em Odontologia Restauradora - ICT

Ação antimicrobiana da clorexidina gel e líquida e do hipoclorito de sódio

The purpose of this study was to evaluate in vitro antimicrobial activity of chlorhexidine gel and liquid 2%, and 2% sodium hypochlorite on Candida albicans, Enterococcus faecalis, Escherichia coli in root canals. For this, we used 40 human single-rooted teeth were divided into 4 groups (n = 10) according to assist the chemical used: 1) 2% chlorhexidine liquid, 2) 2% chlorhexidine gel, 3) sodium hypochlorite 2%, and 4) physiological saline (control). Content were collected immediately after root canal instrumentation and after 7 days of biomechanical. For the samples was conducted to evaluate the antimicrobial activity and the results were subjected to statistical analysis of Kruskal-Wallis and Dunn's test with a significance of 5%. It was found that chlorhexidine gel and liquid as well as sodium hypochlorite were effective against the microorganisms tested

Influência do uso de hipoclorito de sódio 2,5% e extrato glicólico de sálvia 20% durante o preparo biomecânico sobre a resistência adesiva de retentores intra- radiculares: avaliação através do teste de push-out

The aim of this study it was to evaluate the use of irrigating solution used during root canal preparation on the adhesive cementation of prefabricated fiberglass pins. The bond strength between different regions of the root and the fiberglass pins cemented into the root canal were evaluated by push-out assay. For this study, 36 human teeth were used, all equally prepared. The irrigating solutions used for biomechanical preparation were: saline solution, sodium hypochlorite 2,5% and glycolic extract of salvia 20%. The dual cure resin cement was used for cementation of pins. After cementation, specimens were sectioned to give 3 slices of approximately 2 mm each (cervical, middle and apical), which were submitted to mechanical push-out test. Were performed ANOVA and Tukey's test with a significance level of 5%, for the analysis of mechanical test. Comparing the thirds we observed that the only solution that presented statistical difference in the bond strength was sodium hypochlorite, which showed higher values for the middle and apical third compared with the cervical third, but when compared without taking into consideration the dental thirds, solutions studied showed no differences in bond strength. Therefore, it was concluded that the solutions studied can be used during endodontic treatment without causing harmful effects on the final adhesive restoration

Análise do tempo de ação de medicações intracanais sobre candida albicans, enterococcus faecalis, escherichia cole em canais radiculares

The purpose of this study was to evaluate in vitro the antimicrobial activity of intracanal medications against Candida albicans, Enterococcus faecalis, Escherichia coli present in root canals. It was used 24 single root human teeths, contaminated for 28 days and prepared with physiological saline solution as irrigation solution. The teeth were divided into 2 groups according to the intracanal medication used: 1) 2% gel chlorhexidine, 2) sterile and pyrogen free physiological saline solution. Samples were taken of the root canals immediately after instrumentation, 7 days after intracanal medication and 7 days after removal of intracanal medication. For all samples the antimicrobial activity was performed by plating method. All results were submitted to Mann-Whitney and Dunn's test with significance of 5%. There was significant reduction of microorganisms after instrumentation and the intracanal medication of 2% gel chlorhexidine completely eliminated C. albicans and E. coli, and significantly reduced E. faecalis. It was concluded that 2% gel chlorhexidine as intracanal medication for 7 days was effective on microorganisms

Avaliação in vitro da ação de substâncias químicas auxiliares, clorexidina e medicações intracanais sobre C. albicans, E. faecalis, E. coli e sua endotoxina em canais radiculares: [recurso eletrônico]

The purpose of this study was to evaluate the antimicrobial activity of chlorhexidine gel 2% as auxiliary chemical substance on the biomechanical preparation (BMP) and medication intracanal (ICM) on C. albicans, E. faecalis, E. coli and their endotoxin in root canals. We used 48 single-rooted human teeth divided into four groups according to dressing ICM: 1) Ca(OH)2 + pyrogen-free saline solution; 2) 2% chlorhexidine gel (CLX); 3) Ca(OH)2 + CLX, and; 4) pyrogen-free saline solution (control group). Were collected the contents of root canals to confirm the presence of microorganisms (confirmation), immediately after instrumentation (1st collection), after 7 days of the BMP (2nd collection), after 14 days of the action of ICM (3rd Collection) and 7 days after removal of the ICM (4 th collection). Were performed: the evaluation of antimicrobial activity and the content analysis of endotoxins for all sampling tests. The results were statistically analyzed using Kruskall-Wallis and Dunn tests with a significance of 5%. It was found that the CLX as auxiliary chemical substance has significantly reduced microorganisms confirmation collection when compared. In relation to the neutralization of endotoxin, it was found that the 1st and 2nd collections presented a decrease of 92.03% and 98.10% in mean percentage respectively, when compared to the confirmation collection. In the 3rd and 4th samplings, the Ca (OH)2 + CLX group showed the best results. It was concluded that the BMP and the ICM were able to eliminate the tested microrganisms, however, they were not able to completely eliminate endotoxins root canal

Estudo da anatomia interna dos incisivos laterais superiores pelos métodos radiográficos digital e convencional

The maxillary lateral incisor has a very peculiar and internal morphology that can be influenced by the its external anatomy such as supernumerary roots and ruts deep root, which often hinders an effective endodontic treatment. Generally, this tooth have a single root with one root canal, but teeth with two to four channels can be found. The main canal can present ramifications such as accessory canals, laterals, collaterals, recorrents and apical deltas. All this factors interfere directally on endodonthics treatment. The objective of this work is a study of the internal anatomy of the maxillary lateral incisors by digital and conventional radiographics methods, comparing the results obteined by each method Six hundred upper lateral incisor were used in this study teeth of the collection of the Department of Anatomy ICT – UNESP. All the teeth were radiographed by vestibular face, using a digital radiographic unit(Dabi Atlante). After this step, all the teeth underwent by conventional radiographic technique(Kodak 2200) so that the results obtained were compared. To classify the channel system, it was adopted the classification of Pucci and Reig. All data obtained by digital technique were compared with those obtained in the conventional technique and were subjected to statistical analysis

Monitoramento do perfil microbiológico de dentes com infecção endodôntica primária durante terapia com diferentes medicações intracanal

Pós-graduação em Odontologia Restauradora - ICT

Biocompatibilidade da terapia fotodinâmica: estudo in vitro e in vivo

Pós-graduação em Ciência Odontólogica - FOA

Comparação entre soluções irrigadoras na endodontia: clorexidina x hipoclorito de sodio

The objective of this review was reporting an overview about the properties of two irrigating solutions, solution of sodium hypochlorite and chlorhexidine, used in the treatment of root canals. A solution must submit irrigating antimicrobial action, dissolve waste tissue, promote wetting to facilitate the instrumentation, and present biocompatibility with the adjacent tissues. The sodium hypochlorite used in different concentrations has been the solution of choice among professionals by presenting antimicrobial action and act as a solvent tissue. However, today, chlorhexidine has been widely used as an irrigating solution due to specific properties that enable its use, such as substantivity, antimicrobial effectiveness, and low toxicity. Thus the solution of chlorhexidine with different concentrations is presented as an alternative to irrigating solution for the treatment of root canals.

Incomplete vertical root fracture associated with lateral compaction technique: a microscopic analysis

Objective: This study evaluated the effects of root canal obturation employing lateral compaction technique and spreader load of 1.5 kg on the incidence of complete (CVRF) or incomplete vertical root fractures (IVRF). Material and Methods: Twenty-seven distal roots of extracted human mandibular molars were used. All root canals were prepared by biomechanical step-back technique and obturated by lateral compaction technique. The prepared roots were distributed into two groups: G1- experimental (n = 17) and G2- control (n = 10). During obturation, load of 1.5 kg was applied to a size # 30 finger spreader. Pre- and post-obturation images of the coronal portion of the roots were captured by inverted digital microscopy and analyzed by one trained examiner. Data were evaluated by Fisher’s test (p < 0.05) using GrapH Pad Prism 5.0. Results: No roots exhibited CVRF. All fractures observed before and after obturation were IVRF or “other defects”. In G2 (control group), there was no increase of IVRF number. Interestingly, G1 presented an increase in the IVRF number to 70.59% in the 12 teeth out of 17 teeth studied. The statistical analysis showed that the mean of IVRF increased significantly in G1 when compared to G2 (p < 0.05). Conclusion: The application of a 1.5 kg spreader load during lateral compaction technique does not produce complete vertical root fractures, but may produce incomplete fractures or “other defects”.

Aspectos atuais do tratamento da infecção endodôntica

Endodontics is a science that embodies etiology, diagnosis, prevention, and treatment of apical periodontitis and its repercussion in the organism. Technological resources in diagnosis and root canal negotiation, and new strategies , associated to mainly cleaning and eliminating the infection of the root canal system, shaping, and filling the endodontic space, according to biological bases, will allow us to reach higher standards of post-treatment success.

Avaliação da eficácia de limpeza de quatro agulhas irrigadoras endodônticas

The irrigation of root canals aims to their cleaning and disinfection, improving endodontic treatment success. OBJECTIVE: To investigate the influence of the diameter and type of irrigation needle and the root canal enlargement on the mechanical efficacy of endodontic irrigation. MATERIAL AND METHODS: Twelve human single-rooted mandibular incisors were used. During some instrumentation stages (enlargement by #20, #30, and #40 K file), root canals were filled with radiographic contrast solution mixed to propyleneglycol and zinc oxide. Needles with different diameters and designs were employed: G1 – 23G and lateral opening; G2 – 22G and apical opening; G3 – 30G and lateral opening; G4 – 30G and apical opening. The needles were inserted up to resistance, with 1 mm step-back to avoid root canal obliteration. The irrigation was performed with 2 mL of distilled water. Before and after irrigation/aspiration, teeth were radiographed at bucco-lingual and mesiodistal direction, using a digital radiographic system. Then, root canal areas, before (filled by contrast solution) and after irrigation (remnant of contrast solution), were submitted to image subtraction with Adobe Photoshop CS4 software. Subsequently, the areas were measured by Image Tool 3.0 software, allowing the obtaining of the cleaning percentage for each modality. Data were analysed by using Anova and Tukey's test. The level of significance was set at P < 0.05. RESULTS: For all root canal enlargements, 30G needles (G3 e G4) presented a better cleaning efficacy. In all groups, higher cleaning efficacy percentage was observed at #30 and #40 K file enlargement. CONCLUSION: Regardless their design, thinner needles were more effective; a better cleaning efficacy occurred in more enlarged root canals.