Allelic variants of XRCC1 and XRCC3 repair genes and susceptibility of oral cancer in Brazilian patients

Background: The capacity for DNA repair is essential in maintaining cellular functions and homeostasis; however, this capacity can be altered based on DNA sequence variations in DNA repair genes, which may contribute to the onset of cancer. Many single-nucleotide polymorphisms (SNPs) in repair genes have been found to be associated with oral cancer. The aim of this study was to investigate the relationship between the presence of allelic variants Arg194Trp (rs:1799782) and Arg399Gln (rs: 25487) of XRCC1 gene and Thr241Met (rs: 861539) of XRCC3 gene and susceptibility to oral cancer. We also attempted to correlate the frequencies obtained for each of the SNPs to histopathological parameters. Methods: A case-control study was conducted with genomic DNA from 150 patients with oral squamous cell carcinomas and 150 controls. SNPs were genotyped by RFLP-PCR. Results: The presence of the polymorphic variants of the XRCC1 gene within codon 194 (OR 0.82, 95% CI: 0.44-1.51) and codon 399 (OR 0.94, 95% CI: 0.59-1.50) and within the XRCC3 gene (OR 0.72; 95% CI: 0.45-1.16) were not associated with an increased risk of oral cancer. A combinational analysis of SNPs in both genes indicated no association. The presence of the allelic variants of these two genes had no statistically significant effect on tumor differentiation, lymph node invasion or tumor size. Conclusions: These results suggest that allelic variants of XRCC1 and XRCC3 are not suitable markers for susceptibility to carcinomas of the oral cavity and are also not related to the later stages of such tumors. © 2012 John Wiley & Sons A/S.

Resposta da alface à aplicação de biofertilizante sob dois níveis de irrigação

The use of biofertilizers is interesting for agriculture as being an economical alternative as well as it is environmentally friendly by using organic waste and reducing the application of mineral fertilizers. The aim of this study was to evaluate the effect of biofertilizer doses of bovine origin (biodigester effluent) applied on the ground and two levels of irrigation on lettuce. The experiment was conducted under protection of a greenhouse in pots, applying to the soil different doses of biofertilizer of bovine origin obtained from anaerobic reactor (10, 20, 40 and 60 m3 ha-1) and mineral fertilizer as a witness in two irrigation levels calculated at 50 and 100% of reference evapotranspiration. The lettuce plants were analyzed in their: Height, leaves number, crown diameter, fresh weight and dry weight of shoots. The biofertilizer treatments showed better results than the mineral fertilizer and has increased with increasing doses of biofertilizer, the highest dose (60 m3 ha-1) showed the best results in all variables. For dry, mineral fertilization showed higher values. The irrigation levels had no effect on plant growth.

Recent mouse and rat methods for the study of experimental oral candidiasis

The Candida genus expresses virulence factors that, when combined with immunosuppression and other risk factors, can cause different manifestations of oral candidiasis. The treatment of mucosal infections caused by Candida and the elucidation of the disease process have proven challenging. Therefore, the study of experimentally induced oral candidiasis in rats and mice is useful to clarify the etiopathology of this condition, improve diagnosis, and search for new therapeutic options because the disease process in these animals is similar to that of human candidiasis lesions. Here, we describe and discuss new studies involving rat and mouse models of oral candidiasis with respect to methods for inducing experimental infection, methods for evaluating the development of experimental candidiasis, and new treatment strategies for oral candidiasis. © 2013 Landes Bioscience.

Telenomus remus Nixon Egg Parasitization of Three Species of Spodoptera Under Different Temperatures

Telenomus remus Nixon is a promising biocontrol agent as an egg parasitoid of Spodoptera spp., but the lack of information on the host-parasitoid interactions in this system precludes its applied use in agriculture. Therefore, we studied the parasitism capacity of T. remus on eggs of Spodoptera cosmioides (Walker), Spodoptera eridania (Cramer), and Spodoptera frugiperda (Smith) in a range of temperatures (19, 22, 25, 28, 31, and 34 ± 1°C) under controlled conditions (70 ± 10% RH and 12 h photophase). Egg masses of Spodoptera spp. were offered to a single-mated T. remus female on a daily basis. More than 80% lifetime parasitism on eggs of S. cosmioides, S. frugiperda, and S. eridania was reached from 1 to 5, 1 to 7, and 1 to 9 days, respectively, at temperatures from 19 to 34°C. More than 80% parasitization was obtained at extreme temperatures for all hosts studied. Lifetime parasitization of S. frugiperda, S. cosmioides, and S. eridania was affected by temperature, with the lowest values for S. frugiperda (34°C) and S. cosmioides (19 and 34°C). Parasitization of S. eridania eggs was reduced around 18% at 28 and 31°C, but dropped more severely at 34°C. Parasitoid longevity was reduced as temperature increased. Thus, our data indicated that T. remus might be suitable as a biocontrol agent against S. eridania, S. cosmioides, and S. frugiperda in geographical areas that fit the temperature range studied here, even though T. remus parasitism was reduced at 34°C. © 2013 Sociedade Entomológica do Brasil.

Geochemical and radioactivity characterization of rocks from the Rio Preto (Go) Project

The Rio Preto Project, developed by the extinct Brazilian nuclear state company, Nuclebrás, during the late 70s and early 80s, consisted of basic geological mapping and radiometric characterization by aerogeophysical gamma-ray spectrometry, without channel discrimination, of a surface area of 650 km2 located to the west of the Chapada dos Veadeiros National Park on the northeastern of Goiás State, Brazil, including the confluence area of Claro and Preto Rivers. Additionally, the natural radioelements U, Th and 40K were determined by gamma-ray spectrometry in 300 rock samples from cores of the Rio Preto Project area. The tests were conducted at LABIDRO-Isotopes and Hydrochemistry Laboratory of the Departament of Petrology and Metallogeny (DPM) of the Institute of Geosciences and Exact Sciences, UNESP, in Rio Claro, SP, Brazil. This paper reports the results of petrographic characterization and chemical analyses of major oxides (SiO2, TiO2, Al2O3, Fe2O3, MgO, MnO, K2O, Na2O, CaO and P2O5) for all samples used to determine the natural radioelements present in the region. The organic matter content results obtained by colorimetry are also reported for selected cores of different lithotypes in order to investigate the possible relationship between graphite and the radioelements uranium and thorium. Finally, uranium content and 234U/238U activity ratio data for selected samples of schists and gneisses of the Lower Member of the Ticunzal Formation suggest the influence of weathering processes in the area. © 2012 Sociedade Brasileira de Geofísica.

Aplicação de sílica organicamente modificada em sistema de extração em fase sólida de íons Cu(II), Cd(II) e Pb(II) em meio aquoso

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sofistas e filósofos na administração imperial: o olhar de Eunápio sobre a unidade política do império Romano no século IV D. C

Pós-graduação em História - FCHS

Efeito do aumento da concentração de dióxido de carbono do ar sobre a ferrugem e o crescimento de mudas clonais de eucalipto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Equações volumétricas e produção de madeira em plantios mistos visando à restauração da floresta estacional semidecidual

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de diferentes técnicas de ventilação mecânica sobre o estresse oxidativo pulmonar induzido por lesão pulmonar aguda experimental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos toxicogenômicos tardios de terapias antineoplásicas para linfomas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Diagnóstico das infecções relacionadas a cateter em recém-nascidos e epidemiologia molecular dos Staphylococcus spp. isolados

Pós-graduação em Biologia Geral e Aplicada - IBB

Cardiorespiratory evaluation in pre and post operative moments of laparoscopic cholecystectomy

PURPOSE: To analyze the changes in both respiratory function and cardiopulmonary exercise tests results in patients subjected to laparoscopic cholecystectomy. METHODS: Fifty patients were evaluated (76% women) and the average age was 47.8±14.2 years. All individuals underwent the measurement of spirometry, manovacuometry, 6-minute walk test (6MWT) and stair-climbing test (SCT). All tests were performed at the first (PO1), fifth (PO5) and thirtieth (PO30) postoperative days. RESULTS: BMI average was 28.8±4.8 kg/m2. Sample comprised 68% non-smokers, 20% current smokers, and 12% former smokers. There was no incidence of postoperative complication whatsoever. There was a significant decrease in spirometric values at PO1, but values were similar to the ones of PRE at PO30. Manovacuometry showed alterations at PO1 displaying values that were similar to the ones of PRE at PO30. 6MWT was significantly shorter at until PO5, but at PO30 values were similar to ones of PRE. As for SCT, values were significantly compromised at PO5 and PO30 since they were similar to the ones of PRE. CONCLUSION: Patients submitted to laparoscopic cholecystectomy present a decrease in cardiorespiratory function on the first postoperative moments but there is a rapid return to preoperative conditions.

Obtencao de animais negativos para Circovirus suino 2 oriundos de granjas positivas: estrategia de manejo

O objetivo do trabalho é descrever uma estratégia para a obtenção de animais negativos para o PCV2 oriundos de uma granja positiva para este vírus. Dezesseis leitões foram obtidos de fêmeas que tiveram os títulos de IgG anti-PCV2 e o DNA viral testados durante a gestação. Esses leitões, aos sete e dez dias de idade, foram transferidos para a unidade de pesquisa. Durante o período de 7 e 10 aos 49 e 52 dias de idade, amostras de soro, suabes nasal e fecal foram coletadas, a cada sete dias. Após esse período, três animais permaneceram na unidade de pesquisa e foram acompanhados dos 49 aos 114 dias de idade, com coletas realizadas a cada 28 dias. Não houve diferença significativa (p = 0,317) de viremia entre marrãs (n = 6) e porcas (n = 10). Com relação aos níveis de IgG, observou-se diferença significativa (p = 0,0213) entre porcas e marrãs. Os leitões (n = 16), obtidos de duas fêmeas, foram transferidos para a unidade de pesquisa. Os animais entre 7 e 10 dias e aos 49 e 52 dias de idade apresentaram queda de IgG e ausência de IgM anti-PCV2; e as amostras de soro, suabe nasal e fecal foram negativos para o DNA de PCV2. Após os 49 dias, nos três animais mantidos isolados, a detecção de IgG, IgM e DNA para PCV2 permaneceu negativa. Concluindo, a estratégia de manejo utilizada permitiu obter suínos negativos para PCV2 oriundo de granjas positivas para o agente.

Compreendendo o significado da liderança para o aluno de graduação em enfermagem: uma abordagem fenomenológica

This study aimed at understanding the meaning of leadership to undergraduate nursing students and the expectation related to their professional practice. Phenomenology was used as theoretical framework. Fifteen undergraduate nursing students were recruited as subjects and answered the following question: What do you understand by leadership, and how can it be applied in your professional practice? The topics which were revealed and analyzed, Leadership Styles and Leadership Exercise, enabled us to understand that the meaning attributed to leadership is unveiled as a dynamic process, and the style adopted is the form to lead a team; therefore, an ideal leadership style does not exist. In teaching, the leadership style began to be discussed when the participant forms of personnel management were approached. In leadership practice, the dissociation between leadership theory and practice is emphasized, pointing out that integration with practice is relevant for leadership learning.