Sistemas de preparo e resistência à penetração e densidade de um Latossolo Vermelho eutrófico em cultivo intensivo e pousio

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Distribuição longitudinal de sementes de soja e características físicas do solo no plantio direto

As técnicas do plantio direto são complementares às atuais técnicas de conservação do solo, que envolvem menor mobilização e permanência de cobertura vegetal na superfície do solo. O trabalho foi realizado na UNESP - Jaboticabal, de novembro de 2003 a março de 2004, e teve como objetivo avaliar o efeito de culturas, manejos e marchas do trator na semeadura da soja no plantio direto. O delineamento utilizado para instalação do experimento foi em blocos ao acaso, no esquema de parcelas subsubdivididas, com quatro repetições. Foram analisados: estande inicial, distribuição longitudinal de plantas, resistência mecânica à penetração, densidade e teor de água no solo. O estande inicial não foi influenciado pelos tratamentos. A distribuição longitudinal das plantas foi afetada pela marcha do trator. A resistência do solo à penetração sofreu efeito apenas na camada de 0,20 a 0,25 m na interação culturas, manejos e marchas do trator. A interação culturas e manejos na camada de 0,10 a 0,20 m para densidade indicou a presença de resistência maior do que 0 a 0,10 m e de 0,20 a 0,30 m. O teor de água no solo foi menor nas camadas superficiais do solo, influenciado pelos manejos.

Efeitos da roda compactadora de semeadoras sob cargas verticais na deformação do solo com dois teores de água

O presente trabalho foi conduzido na Pista de Ensaios de Semeadura do Laboratório de Máquinas e Mecanização Agrícola (LAMMA) da UNESP/Jaboticabal - SP, para o estudo da ação da roda compactadora de semeadoras sob cargas verticais, na deformação do solo, com dois teores de água. Os tratamentos consistiram da combinação de dois teores de água e seis cargas verticais, totalizando 12 tratamentos, com três repetições, em dois ensaios, analisando-se a resistência mecânica do solo à penetração e a deformação do solo provocada pela roda compactadora. A roda compactadora utilizada era de alumínio, com massa de 6,4 kg, 40 cm de diâmetro e 10 cm de largura, sob a ação de cargas verticais de 63; 161; 259; 357; 455 e 553 N, obtidas acoplando-se lastros de chumbo sobre a roda compactadora, sendo os teores de água do solo de 15,4 e 9,2%. Os resultados permitem concluir que o teor de água do solo tem grande influência na deformação e compactação do solo, que aumentam proporcionalmente com as cargas verticais sobre a roda compactadora, e que, quanto maior o teor de água do solo, mais suscetível o mesmo fica à compactação e deformação.

Diallel analysis of maize resistance to Phaeosphaeria maydis

Em programas de melhoramento visando resistência genética a doenças, a estimativa de parâmetros genéticos que governam a resistência permite direcionar a introdução de resistência em germoplasmas. O objetivo deste trabalho foi estimar os efeitos heteróticos, a capacidade geral (CGC) e específica (CEC) de combinação, utilizando-se de dois métodos de avaliação da resistência, à Phaeosphaeria maydis através da análise dialélica de 36 híbridos F1 e de suas nove linhagens genitoras, em experimentos conduzidos em três ambientes. Foi utilizado um delineamento experimental em blocos casualizados com três repetições e a parcela experimental foi representada por uma fileira de 5 m. As diferenças entre as estimativas da capacidade de combinação, em diferentes ambientes e para os dois métodos de avaliação, apresentaram efeitos significativos (P < 0.01) para ambientes (E), CGC e CGC x E. O efeito de CEC e a interação CEC x E não foi significativa para os dois métodos de avaliação. Os efeitos de CGC foram mais importantes que CEC nesse conjunto de linhagens, sugerindo que efeitos genéticos aditivos são mais importantes como fonte de variação para resistência a esta doença. Efeitos heteróticos para resistência foram estimados, sendo possível identificar combinações híbridas específicas entre linhagens com alto potencial para o controle genético deste patógeno. Resultados para os dois métodos de avaliação foram praticamente idênticos, embora o método PI seja de maior praticidade de uso.

Genetics of resistance to soybean cyst nematode, Heterodera glycines ichione (Race 3), in a brazilian soybean population

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Combining ability of Phaseolus vulgaris L. for resistance to common bacterial blight

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identification of QTLs associated with citrus resistance to Phytophthora gummosis

Citrus gummosis, caused by Phylophthora spp., is an important citrus disease in Brazil. Almost all citrus rootstock varieties are susceptible to it to some degree, whereas resistance is present in Poncirus trifoliata, a closely related species. The objective of this study was to detect QTLs linked to citrus Phylophthora gummosis resistance. Eighty individuals of the F, progeny, obtained by controlled crosses between Sunki mandarin Citrus sunki (susceptible) and Poncirus trifoliata cv. Rubidoux (resistant), were evaluated. Resistance to Phytophthora parasitica was evaluated by inoculating stems of young plants with a disc of fungal mycelia and measuring lesion lengths a month later. Two QTLs linked to gummosis resistance were detected in linkage groups I and 5 of the P. trifoliala map, and one QTL in linkage group 2 of the C sunki map. The phenotypic variation explained by individual QTLs was 14% for C sunki and ranged from 16 to 24% for P. trifoliala. The low character heritability (h(2) = 18.7%) and the detection of more than one QTL associated with citrus Phytophthora gummosis resistance showed that inheritance of the resistance is quantitative.

Clinical features and genetic analysis of four Brazilian kindreds with resistance to thyroid hormone

Objective Resistance to thyroid hormone (RTH) is a dominantly inherited syndrome of reduced tissue responsiveness to thyroid hormone usually due to mutations located in the ligand-binding domain and adjacent hinge region of the thyroid hormone receptor beta (TR beta). In the present report we describe the clinical and laboratory characteristics and the genetic analysis of patients with this rare disorder from a Brazilian population.Patients Four unrelated Brazilian families with diagnosis of RTH were studied. Age at diagnosis varied from 14 months to 29 years.Results All affected individuals were clinically euthyroid, except for one patient who presented immediately after birth with hyperthyroidism. All individuals had tachycardia and goitre, elevated concentrations of free thyroid hormones and reduced sensitivity to thyroid hormone. Direct sequencing analysis of the TR beta gene revealed four previously reported mutations: c.949G -> A, c.1313G -> A, c.1357C -> A and c.1358dupC in families A, B, C and D, respectively.Conclusion the present report shows that the frequent mutations described in the thyroid hormone receptor worldwide are also present in the Brazilian population, which is characterized by a variable ethnic background.

Identification and Analyses of Candidate Genes for Rpp4-Mediated Resistance to Asian Soybean Rust in Soybean

Asian soybean rust is a formidable threat to soybean (Glycine max) production in many areas of the world, including the United States. Only five sources of resistance have been identified (Resistance to Phakopsora pachyrhizi1 [Rpp1], Rpp2, Rpp3, Rpp4, and Rpp5). Rpp4 was previously identified in the resistant genotype PI459025B and mapped within 2 centimorgans of Satt288 on soybean chromosome 18 (linkage group G). Using simple sequence repeat markers, we developed a bacterial artificial chromosome contig for the Rpp4 locus in the susceptible cv Williams82 (Wm82). Sequencing within this region identified three Rpp4 candidate disease resistance genes (Rpp4C1-Rpp4C3 [Wm82]) with greatest similarity to the lettuce (Lactuca sativa) RGC2 family of coiled coil-nucleotide binding site-leucine rich repeat disease resistance genes. Constructs containing regions of the Wm82 Rpp4 candidate genes were used for virus-induced gene silencing experiments to silence resistance in PI459025B, confirming that orthologous genes confer resistance. Using primers developed from conserved sequences in the Wm82 Rpp4 candidate genes, we identified five Rpp4 candidate genes (Rpp4C1-Rpp4C5 [PI459025B]) from the resistant genotype. Additional markers developed from the Wm82 Rpp4 bacterial artificial chromosome contig further defined the region containing Rpp4 and eliminated Rpp4C1 (PI459025B) and Rpp4C3 (PI459025B) as candidate genes. Sequencing of reverse transcription-polymerase chain reaction products revealed that Rpp4C4 (PI459025B) was highly expressed in the resistant genotype, while expression of the other candidate genes was nearly undetectable. These data support Rpp4C4 (PI459025B) as the single candidate gene for Rpp4-mediated resistance to Asian soybean rust.

Identification of quantitative trait loci affecting resistance to gastrointestinal parasites in a double backcross population of Red Maasai and Dorper sheep

A genome-wide scan for quantitative trait loci (QTL) affecting gastrointestinal nematode resistance in sheep was completed using a double backcross population derived from Red Maasai and Dorper ewes bred to F1 rams. This design provided an opportunity to map potentially unique genetic variation associated with a parasite-tolerant breed like Red Maasai, a breed developed to survive East African grazing conditions. Parasite indicator phenotypes (blood packed cell volume PCV and faecal egg count FEC) were collected on a weekly basis from 1064 lambs during a single 3-month post-weaning grazing challenge on infected pastures. The averages of last measurements for FEC (AVFEC) and PCV (AVPCV), along with decline in PCV from challenge start to end (PCVD), were used to select lambs (N = 371) for genotyping that represented the tails (10% threshold) of the phenotypic distributions. Marker genotypes for 172 microsatellite loci covering 25 of 26 autosomes (1560.7 cm) were scored and corrected by Genoprob prior to qxpak analysis that included BoxCox transformed AVFEC and arcsine transformed PCV statistics. Significant QTL for AVFEC and AVPCV were detected on four chromosomes, and this included a novel AVFEC QTL on chromosome 6 that would have remained undetected without BoxCox transformation methods. The most significant P-values for AVFEC, AVPCV and PCVD overlapped the same marker interval on chromosome 22, suggesting the potential for a single causative mutation, which remains unknown. In all cases, the favourable QTL allele was always contributed from Red Maasai, providing support for the idea that future marker-assisted selection for genetic improvement of production in East Africa will rely on markers in linkage disequilibrium with these QTL.

Evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to pyrazinamide

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Molecular mapping of two loci that confer resistance to Asian rust in soybean

Asian soybean rust (ASR) is caused by the fungal pathogen Phakopsora pachyrhizi Sydow & Sydow. It was first identified in Brazil in 2001 and quickly infected soybean areas in several countries in South America. Primary efforts to combat this disease must involve the development of resistant cultivars. Four distinct genes that confer resistance against ASR have been reported: Rpp1, Rpp2, Rpp3, and Rpp4. However, no cultivar carrying any of those resistance loci has been released. The main objective of this study was to genetically map Rpp2 and Rpp4 resistance genes. Two F(2:3) populations, derived from the crosses between the resistant lines PI 230970 (Rpp2), PI 459025 (Rpp4) and the susceptible cultivar BRS 184, were used in this study. The mapping populations and parental lines were inoculated with a field isolate of P. pachyrhizi and evaluated for lesion type as resistant (RB lesions) or susceptible (TAN lesions). The mapping populations were screened with SSR markers, using the bulk segregant analysis (BSA) to expedite the identification of linked markers. Both resistance genes showed an expected segregation ratio for a dominant trait. This study allowed mapping Rpp2 and Rpp4 loci on the linkage groups J and G, respectively. The associated markers will be of great value on marker assisted selection for this trait.

Qualidade física de um Latossolo Vermelho em recuperação há dezessete anos

The bulk density and resistance to penetration as indicators of recovery were studied with the objective to investigate the physical quality of a Red Latosol (Oxisol) under restoration for 17 years using green manures, soil correction, gypsum and pasture. The experimental design was a completely randomized with nine treatments and four replications. The treatments were: control (tilled soil without crop); Stizolobium aterrium; Cajanus cajan until 1994 and then substituted by Canavalia ensiformis; lime+S. aterrimum; lime+C. cajan until 1994 and then substituted by C. ensiformis; lime+gypsum+S. aterrimum; lime+ gypsum+C. cajan until 1994 and then substituted by C. ensiformis, the treatments were installed in 1992 and remained for seven years, in 1999 Brachiaria decumbens was planted; two other controls (native vegetation and exposed soil) were also used to compare. Bulk density, resistance to penetration and soil moisture were evaluated. The results were analyzed performing analysis of variance and Tukey test at probability of 5% to compare means. The treatments of reclamation are improving the physical qualities of soil and the results of treatment with C. cajan/C. ensiformis, lime and gypsum attained physical conditioning similar to soil under natural conditions.