Um estudo introdutório da literatura de João Carlos Marinho

Pós-graduação em Letras - FCLAS

Avaliação de diferentes espaçamentos, altura e épocas de poda na cultura do pinhão manso na região de Selvíria-MS

Pós-graduação em Agronomia - FEIS

Isolation, Culture and Differentiation of Buffaloes Bone Marrow Mesenchymal Stem Cells Obtained from the Coxal Tuberosity

Currently, much attention has been devoted to the renewal of knowledge about Stem Cells and Cell Therapy in domestic species. In this sense, the present work aimed to develop a methodology for collecting, processing and cultivation of mesenchymal stem cells obtained from bone marrow of coxal tuberosity in buffaloes. The collection was performed using a Komiyashiki needle, which was introduced in the coxal tuberosity and the bone marrow aspirated into a heparinized syringe with the aid of negative pressure. Directly after collection samples were processed at the laboratory at FMVZ - UNESP. The samples took approximately 32 days to reach 80% confluence, when the first passage and differentiation was performed. To confirm the mesenchymal origin, cells were induced to differentiate into adipogenic and osteogenic lineages. Samples showed morphological changes during differentiation protocol, but not all presented production of extracellular deposits of calcium or intracellular fat droplets, observed after staining with Alizarin Red and Oil Red respectively. Compared with the material obtained from other species and processed in the same laboratory, the primary culture was longer. Therefore, more studies are needed to standardize the age of animals used and to test other inducers of cell differentiation.

Automação do manejo alimentar na engorda de tilápias criadas em tanque-rede

Pós-graduação em Zootecnia - FMVZ

Custo de produção e lucratividade da cultura do amendoim no município de Jaboticabal, São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A perspectiva metodológica da resolução de problemas: um estudo sobre enunciados de situações matemáticas na EJA

In this paper we discuss the importance of a methodological perspective of solving problems as a sustaining process of teaching mathematics situated on the perspective of concept formation. Organizing a significant didactic situation for students imposes the need to study the interaction between them and the teacher and between them and their mathematical knowledge, learning environment in which the mere transmission of content gives way to contextualization, to historicizing and handling of topics from intuitive and everyday situations for the student. Thus, we understand mathematics as a fundamental language for the creation of theoretical thinking as a whole. We made use of documental analysis and classroom situations aiming at the use of instructional procedure related to the resolution of problems with the purpose of overcoming some representations about the process of teaching and learning mathematics which is strongly marked by imitative-repetitive algorithmic procedures. Considering mathematics as an investigation discipline, we point out renewal prospects for the curricula of this discipline, which are concrete in the movement of cultural action of the school itself as the cell generating discussion.

Extracellular matrix remodeling of the testes through the male reproductive cycle in Teleostei fish

During the fish reproductive cycle, testes undergo morphological changes related to germinal epithelium and remodeling of extracellular matrix components (ECM). ECM is degraded mainly by action of matrix metalloproteinases (MMPs). Due to the natural renewal of ECM in fish testes, we choose Pimelodus maculatus to study remodeling of ECM throughout reproductive cycle, using picrosirius (to identify type I, II, III collagen) and reticulin (type III collagen), and to immunolocalize MT1-MMP (membrane type 1-matrix metalloproteinase) and MMP-2 in testis cells. Testes were classified in four reproductive phases: regenerating, development, spawning capable and regressing. Picrosirius and reticulin demonstrated a differential distribution of total collagen fibers during the reproductive cycle. Immunohistochemistry showed MT1-MMP only in acidophilic granulocyte cells mainly inside blood vessels, in connective tissue of capsule close to the germinal compartment, and also infiltrated in interstitial connective tissue. MMP-2 was detected in fibroblast and endothelial cells of interstitial and capsule blood vessels, in epithelial cells of capsule, and in acidophilic granulocyte cells at same description for MT1-MMP. The fish testes ECM were remodeled throughout reproductive cycle in according to morphophysiological alterations. During reproductive season (spawning capable), the interstitium increased in total collagen fibers (type I, II, III). After spermiation period (regression and regenerating), the amount of collagen fibers decreased in response to action of MMPs on collagen degradation and other interstitial components (not assessed in this study). MMPs seem to be indispensable components for natural cyclic events of ECM remodeling of fish testes and for guarantee tissue homeostasis throughout reproductive cycle.

Feed digestibility and productive performance of bullfrogs raised in cages and fed in different periods and high frequency

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A new probabilistic canopy dynamics model (SLCD) that is suitable for evergreen and deciduous forest ecosystems

There are strong uncertainties regarding LAI dynamics in forest ecosystems in response to climate change. While empirical growth & yield models (G&YMs) provide good estimations of tree growth at the stand level on a yearly to decennial scale, process-based models (PBMs) use LAI dynamics as a key variable for enabling the accurate prediction of tree growth over short time scales. Bridging the gap between PBMs and G&YMs could improve the prediction of forest growth and, therefore, carbon, water and nutrient fluxes by combining modeling approaches at the stand level.Our study aimed to estimate monthly changes of leaf area in response to climate variations from sparse measurements of foliage area and biomass. A leaf population probabilistic model (SLCD) was designed to simulate foliage renewal. The leaf population was distributed in monthly cohorts, and the total population size was limited depending on forest age and productivity. Foliage dynamics were driven by a foliation function and the probabilities ruling leaf aging or fall. Their formulation depends on the forest environment.The model was applied to three tree species growing under contrasting climates and soil types. In tropical Brazilian evergreen broadleaf eucalypt plantations, the phenology was described using 8 parameters. A multi-objective evolutionary algorithm method (MOEA) was used to fit the model parameters on litterfall and LAI data over an entire stand rotation. Field measurements from a second eucalypt stand were used to validate the model. Seasonal LAI changes were accurately rendered for both sites (R-2 = 0.898 adjustment, R-2 = 0.698 validation). Litterfall production was correctly simulated (R-2 = 0.562, R-2 = 0.4018 validation) and may be improved by using additional validation data in future work. In two French temperate deciduous forests (beech and oak), we adapted phenological sub-modules of the CASTANEA model to simulate canopy dynamics, and SLCD was validated using LAI measurements. The phenological patterns were simulated with good accuracy in the two cases studied. However, IA/max was not accurately simulated in the beech forest, and further improvement is required.Our probabilistic approach is expected to contribute to improving predictions of LAI dynamics. The model formalism is general and suitable to broadleaf forests for a large range of ecological conditions. (C) 2014 Elsevier B.V. All rights reserved.

Lean Six sigma aplicado a uma empresa do setor de embalagens

With the advent of a global economy, Lean Six Sigma meets the demand of new production process that creates products within the customer’s specification without any wastage. The objectives of this work is to use the concepts Lean Manufacturing and Six Sigma parallel to minimize costs, increase the customer base, reduce the delivery time which implies an increase in the turnover of products on the shelves in a packaging company. We used the DMAIC method to continue working. The method DMAIC indicated the main failure modes that were fixed in the step improve. At this stage the tools of Lean Manufacturing gain tremendous value by optimizing processes with defects. The conclusion involves an increase in monthly billing, a corporate restructuring and, finally, a renewal in how to conduct the work of management

Análise do plano de radioproteção em radioterapia

Ionizing radiation is used nowadays in various sectors such as agriculture, industry and medicine. The main specialties of medicine which use radiation are the diagnostic radiology, nuclear medicine and radiotherapy. Radiotherapy is a therapeutic modality that is a well established feature for the treatment of malignant disease or not. However, the inadvertent use of ionizing radiation can produce deleterious effects that result in sequels that compromise the welfare of the people involved. The analysis of radiological protection emphasizes the importance of avoiding inappropriate exhibitions aimed at protecting the health of patients, the professionals involved and the general public. The basic principles of radioprotection are justification, optimization and restriction for individual dosage. The departments of radiotherapy are regulated in accordance with specific technical standards of the National Commission of Nuclear Energy (CNEN), which during the inspection for issue and renewal of the authorization of operation requires the submission of a radioprotection plan, this document that requires great demand of time, and has generated much debate among professionals in medical physics, given the difficulties encountered in their preparation. After examining the radioprotection plan of some radiotherapy services, as suggested in order to guide those responsible for drawing up these plans, especially beginners in the career of the physics of radiation, this paper presents a model plan that is in line radioprotection it requires the Technical Standards of CNEN and can easily be the reality of appropriate services

Diferenciação miogênica “in vitro” de células tronco mesenquimais murinas de diferentes origens

Muscular dystrophy refers to a group of more than 30 genetical disorders characterized by progressive weakness and degeneration of the skeletal muscle. No effective therapy is available at present. Recent studies have reported that the transplantation of stem cells can offer an important potential therapy for genetic diseases. Adult bone marrow mesenchymal stem cells have been identified as a nonhematopoietic stem cell population capable of self-renewal with the ability to differentiate into many cell lineages, including bone, fat, cartilage and connective tissue. Because of their similarity with muscle progenitor cells, when they are injected in affected individuals, they are able to migrate into areas of skeletal muscle degeneration and participate in the regeneration process. The adipose tissue represents an alternative source of MSCs that, as the MSCs derived from bone marrow, are capable of in vitro differentiation into osteogenic, adipogenic, myogenic and chondrogenic lineages. The objective of this project is to investigate the “in vitro” myogenic potential of mesenchymal stem cells derived from murine bone marrow and adipose tissue. Four experimental groups were analyzed: mice from lineages Lama2dy-2J/J and C57black and, C2C12 lineage cells and transformed C2C12 expressing the eGFP protein. MSCs cultures were obtained by flushing the bone marrow femurs and tibials with α-MEM or by the subcutaneous and inguinal fat from the mice. Their characterization was done by flow cytometry and in vitro differentiation. Muscle differentiation was studied through the analysis of the expression of transcriptional factors involved in muscle differentiation and/or the presence and amount of specific proteins from muscle differentiated cell. The pluripotency from bone marrow MSCs of the two lineages was evidenced and, in the muscular differentiation... (Complete abstract click electronic access below)

Estudo das políticas de obtenção dos assentamentos de reforma agrária no Brasil entre 1985 e 2009

Understanding the territorial transformations is important to the geographic knowledge, especially when it is related to structural questions as agrarian one. In this way, the land acquisition to agrarian reform enables us to understand some of these transformations, represented by territorialization of landless workers in rural settlements, that are concrete results of the struggle for land that boost the policies of agrarian reform in our country. The renewal of the agrarian structure is fundamental to the concept of land reform and also provides that advances social, political, cultural, economic, etc. Classified as a country with rates of the highest land concentration in the world, Brazilian government attempts to base his actions to take in the expropriation of land the main character of the land reform. However, new policies have been gradually used to obtaining the rural settlements, in a process that Fernandes (2010) called the reconceptualization of land reform. Starting in 1985, Brazil has 63% of the beneficiaries of land reform policies settled on expropriated land, the rest is a result of incorporation policies such as land regularization, of land reform and land purchase. Such policies generate changes in agrarian structure, that is the goal of land reform, but don´t concentrate to the land ownership. This attitude of the state in coping with the agrarian question is his response to the pressures of capitalist agriculture represented by landowners and agribusiness, coping catching an intense and uneven territorial dispute with peasant agriculture, in an attempt to impose its model of development for the field. The aimed of this present study is to understand better the land reform acquisition and its implications in the Brazilian territory, in order to understand it in their multiplicity.

Influência da luz LED em culturas de células-tronco mesenquimais in-vitro

Stem cells are defined as cells capable of self-renewal and differentiation into specialized cells when submited to external signalings in the enviroment. Among adult stem cells, mesenchymal cells occupy an important position because they can differentiate into mesodermal cells such as osteoblasts, adipocytes and chondrocytes. Cell therapy consists in the use of mesenchymal stem cells (MSC) in the treatment of degenerative diseases and harmed tissue reconstruction. Due to the longstanding and costly procedure for cultivation of MSC, it was proposed the use of low power light sources, such as light emitting diodes (LED), to optimize these factors. Recent works have shown a series of results from the influence of LED light on biological tissues such as increased rate of cell proliferation, increased RNA, DNA and ATP synthesis rate. The purpose of this study is to compare the biomodulator effect of LED light set at wavelengths 630nm ± 10nm and 805nm ± 10nm on the mesenchymal stem cells proliferation. For this, the mesenchymal stem cells culture adopted the procedure used in the Departament of Animal Reproduction and Veterinary Radiology of the Faculty of Veterinary Medicine and Animal Sciences of Botucatu. MSC were obtained from an adult horse bone marrow, and isolated by density gradient separation, with the FICOLL reagent and by centrifugation. The pellet containing the stem cells was removed and these were placed in low glucose DMEM culture medium, containing 10% fetal calf serum and antibiotics. The material was observed daily by inverted microscopy for monitoring the progression of the cells and subsequently the amount of cells were counted in a Neubauer counting chamber. The amount of MSC was obtained by cell culture seeded in 24 wells culture plate and segregated into three distinct groups: Group 1 was irradiated with wavelength set at 630nm ± 10 nm, Group... (Complete abstract click electronic access below)

Professor coordenador na formação contínua de professores: um estudo em escolas do município de Limeira

Pós-graduação em Educação - IBRC