Cryopreservation, early seedling development, and genetic stability of Oncidium flexuosum Sims

An efficient cryopreservation protocol was developed for mature seeds of Oncidium flexuosum Sims. Seed morphology, protocorm formation, and early seedling development were also assessed. The effects of phloroglucinol and Supercool X-1000® as cryoprotectant additives in the vitrification solution were investigated. Dehydration using the plant vitrification solution 2 (PVS2) for 60 and 120 min prior to immersion in liquid nitrogen promoted the highest frequency of in vitro seed germination 6 weeks following culture on half-strength Murashige and Skoog (1/2 MS) medium. Mature seeds submitted to vitrification for 120 min in PVS2 and 1 % phloroglucinol at 0 °C enhanced germination by 68 %, whereas in PVS2 and 1 % Supercool X-1000® germination was just moderately enhanced (26 %). In vitro-germinating seedlings developed healthy shoots and roots without the use of plant growth regulators. After 6 months of growth, there were no differences between in vitro- and ex vitro-grown seedlings for various phenotypic characteristics, including shoot length, number of leaves, number and length of roots, and fresh and dry weight. Seedlings were transferred to greenhouse conditions and successfully acclimatized, further developing into normal plants with over 90 % survival. Comparative analysis of seedlings from control and vitrified seeds using flow cytometry indicated that no change in ploidy levels occurred as a result of cryopreservation, therefore maintaining seedlings genetic stability. In this study, vitrification with PVS2 for 120 min with the addition of 1 % phloroglucinol offers a simple, safe, and feasible protocol for cryopreservation of O. flexuosum mature seeds. © 2013 Springer Science+Business Media Dordrecht.

Teor de ácido cianídrico e taxa de crescimento do híbrido de sorgo x capim sudão durante o outono

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nutrient accumulation in 'Fantasy' net melon cultivated on substrate

Knowledge of the most essential nutrients for plant growth facilitates the efficient handling of its nutrition, especially when they are grown on a substrate supported by fertigation. The objective of this study was to determine the accumulation of nutrients in net melon grown on a substrate and understand the relationship between mineral nutrition and plant growth. The Fantasy hybrid was cultivated in pots containing a substrate consisting of a mixture of sand and peanut shells (ratio, 1:1). Determination of nutrient accumulation was performed in 6 seasons. The substrate was chemically characterized before and after cultivation. Harvesting occurred 78 days after transplantation, resulting in an average yield of 70,120 kg·ha-1. Substrate analysis showed a small increase in nutrient levels by the end of cultivation. The order of nutrient accumulation was as follows: N>Ca>K>P>Mg>S>B> Fe>Mn>Zn>Cu.

Growth of euterpe oleracea seedlings under different color nets and light conditions

Euterpe oleracea, a native palm to the Amazon, has recognized trading importance because of its fruits and heart of palm. However, it still requires general information on its propagation and cultivation. As light is essential for plant growth, this work aimed to study the effect of different color nets and light conditions on the initial growth of E. oleracea, with the objective to obtain high quality seedlings. The experiment was conducted in Jaboticabal County, São Paulo State, Brazil. The experimental design was entirely randomized, with six treatments (six light conditions: red net with 50% of shading - red Chromatinet®; blue net with 50% of shading - blue Chromatinet®; black nets with 70, 50 or 30% of shading; and full sun) and six replications (six evaluation periods: 30, 60, 90, 120, 150 and 180 days after the beginning of the experiment). Each plot consisted of four pots with one plant per pot, resulting in 144 plants. Seedling growth was monthly evaluated by collecting four plants per replication until the end of the experiment. Shoot length, root length, stem diameter, leaf number, leaf area and root and shoot dry matter were evaluated. The best quality seedlings of E. oleracea were obtained when they were cultivated under the black net with 50% of shading.

Crescimento, produtividade, acúmulo e exportação de nutrientes em cultivares de batata (Solanum tuberosum L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Disponibilidade de fósforo em função da aplicação de calcário e silicatos em solos oxídicos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Adubação fosfatada e lodo de esgoto compostado na nutrição da mamoneira de porte baixo, cultivada sob calagem e silicatagem

Pós-graduação em Agronomia (Agricultura) - FCA

Germinação de sementes de Passiflora setacea DC: temperatura, luz e reguladores vegetais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cálcio e ethephon no desenvolvimento e produção de óleo essencial de menta (Mentha Piperita L.), cultivada em solução nutritiva

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Compostos fenólicos e atividade antioxidante em Salvia officinalis L. micropropagada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação energética e econômica da substituição de tubetes de plástico por tubetes biodegradáveis na produção de mudas de aroeira - Schinus terebinthifokius Raddi

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento de larenjeira 'Pera' Citrus Sinensis (L.) Osbeck enxertada em limoeiro 'Cravo' (Citrus Limonia) e cultivada em pó de balsalto

Pós-graduação em Ciências Biológicas (Botânica) - IBB

Desenvolvimento de plantas jovens de Annona emarginata (SHLTDL.) H. Rainer (Araticum-de-terra-fria) cultivadas e solução nutritiva

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de fitomassa, teor e composição química do óleo essencial de Baccharis dracunculifolia DC. em função da adubação orgânica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Doses de nitrogênio no desenvolvimento de girassol ornamental (Helianthus annuus L.) de vaso

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)