Development of a method for sampling and determination of corrosion inhibitors in modified atmospheres

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Synthesis and characterization of cross-linked molecularly imprinted polyacrylamide for the extraction/preconcentration of glyphosate and aminomethylphosphonic acid from water samples

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Method validation using weighted linear regression models for quantification of UV filters in water samples

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

HPLC-DAD method for metabolic fingerprinting of the phenotyping of sugarcane genotypes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Microdissection testicular sperm extraction causes spermatogenic alterations in the contralateral testis

Testicular sperm extraction (TESE) associated with intracytoplasmic sperm injection has allowed many men presenting non-obstructive azoospermia to achieve fatherhood. Microdissection TESE (microTESE) was proposed as a method to improve sperm retrieval rates in these patients; however, there have been failures. Little is known about whether microTESE leads to spermatogenic alterations in the contralateral testis. We assessed histological outcomes of experimental microTESE in the contralateral testis of adult male rabbits. Nine adult male rabbits were divided into three groups: control (testicular biopsy to observe normal histological and morphometric values), sham (incision of the tunica vaginalis, and a contralateral testicular biopsy to observe histological and morphometric patterns, 45 days later), and study (left testicular microTESE, and a right testicular biopsy to observe histological and morphometric patterns, 45 days later). Sections were assessed by calculating Johnsen-like scores, and measuring total tubule diameter, lumen diameter and epithelial height. The results were compared using ANOVA and Bonferroni's statistical analysis. Morphometric evaluation of the seminiferous tubules did not demonstrate differences between the three groups. However, microTESE caused spermatogenic alterations, leading to maturation arrest in the contralateral testis.

Feature extraction in pressure signals for leak detection in water networks

Techniques based on signal analysis for leak detection in water supply systems typically use long pressure and/or flow data series of variable length. This paper presents the feature extraction from pressure signals and their application to the identification of changes related to the onset of a leak. Example signals were acquired from an experimental laboratory circuit, and features were extracted from temporal domain and from transformed signals. Statistical analysis of features values and a classification method were applied. It was verified the feasibility of using feature vectors for distinguish data acquired in the absence or presence of a leak.

High precision and selectivity for quantitation of enrofloxacin and ciprofloxacin in five chicken tissues using solid phase extraction and ESI LC-MS/MS for application in monitoring residues

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Development of a new clean methodology with ultrasound-assisted extraction for analysis of sodium in pet foods

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Semiautomatic object-space road extraction combining a stereoscopic image pair and a TIN-Based DTM

In this letter, a semiautomatic method for road extraction in object space is proposed that combines a stereoscopic pair of low-resolution aerial images with a digital terrain model (DTM) structured as a triangulated irregular network (TIN). First, we formulate an objective function in the object space to allow the modeling of roads in 3-D. In this model, the TIN-based DTM allows the search for the optimal polyline to be restricted along a narrow band that is overlaid upon it. Finally, the optimal polyline for each road is obtained by optimizing the objective function using the dynamic programming optimization algorithm. A few seed points need to be supplied by an operator. To evaluate the performance of the proposed method, a set of experiments was designed using two stereoscopic pairs of low-resolution aerial images and a TIN-based DTM with an average resolution of 1 m. The experimental results showed that the proposed method worked properly, even when faced with anomalies along roads, such as obstructions caused by shadows and trees.

Optimization of extraction conditions and antioxidant activity of Solanum lycocarpum fruits

Antioxidants are substances that may protect cells from the damage caused by unstable molecules known as free radicals. The capacity of natural antioxidant from phytochemical has increase attention from researchers and public. However, the extraction process is affecting the activity and the bioavailability of bioactive compounds. The Solanum lycocarpum is a plant of the Brazilian “cerrado”, popularly used as a hypoglycemic, hypocholesterolemic and control of metabolic diseases. Its effects are attributed to the presence of several glycoalkaloids (solamargine, solasonina) and solasodine. Therefore, the purpose of this communication was, investigate the optimization of extraction condition and evaluation of antioxidant activity from fruits of Solanum lycocarpum. The extracts were obtained using different solvent systems, i.e., water, 50% ethanol, ethanol absolute and ethyl ether (1:10 and 1:20) and different extraction processes: maceration with constant agitation at room temperature, maceration with constant agitation and heating at 30°C and ultrasound. The extracts were characterized by the amount of material extracted (1, 6 and 24 h) and the action of antioxidant activity by DPPH method. The results showed that the polar solvent (50% ethanol) and extractive process maceration with agitation to ambient temperature showed higher contents of extractable of fruits of S. lycocarpum (3.4 g %) and also showed higher antioxidant activity (88.57±2.41% de inhibition). This action whether the presence of glycoalkaloids (solamargine, solasonine and solasodine) in fruits S. lycocarpum which are polar compounds and may explain this increased antioxidant action of this extract.

Development and validation of an LC-MS/MS method for quantitative analysis of mirtazapine in human plasma

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)