Os papéis dos RNAs não codificantes no desenvolvimento embrionário: um panorama geral

The genome of multicellular organisms shows a ruge index of RNA transcripts that are not protein coding. These ncRNAs act in housekeeping and genic regulation, as well in signaling and cell differentiation, crucial events to embryonic and ontogenetic development. Moreover, another events require the orderly expression these transcripts, as in cromossomic inactivation and genomic imprinting process, and their fail may cause several syndromes, malformations, illness and even death of affected individual. This review focus is to present the main acting pathways of ncRNAs already studied, as well to introduce the actual landscape of Dapper gene cluster and its performance in vertebrate development.

Enzimas de biotransformação em tilápia (Oreochromis niloticus) exposta ao hormônio natural 17b-estradiol

Among the sexual hormones the estrogens are receiving major attention due to their biological activity. Such biological response is atributed to the best conformation recognized by their receptors, resulting in maximum responses. The estrogens are also considered responsible for most of disruptor´s effects caused by their presence in aquatic ecosystems. The 17β-estradiol hormone is produced by vertebrates during the reproductive phase of their lives and its presence has been detected in superficial waters. The objective of this study was to evaluate the cause-effect of tilapia exposition to the hormone 17β-estradiol through the analysis of biotransformation enzymes in liver and gills, complemented with the quantification of 17β-estradiol and estrone in water samples collected from fish ponds integrated to swine production. The present study was conducted under laboratory conditions, in a randomized experimental design with three levels of 17β-estradiol (E2) (0, 5, 15 µg L-1), with three replicates. After 7 days of exposure time, liver and gills were extracted to analyze three isoforms of cytochrome P450: EROD, BROD, PROD and the activity of Glutathione S-Transferase (GST). The results showed that the EROD activity (CYP1A), normally induced by the metabolism of aromatic compounds, did not present statistical differences among the treatments exposed to E2, what means that the hormone did not induce isoform 1A in fish under these particular experimental conditions. PROD activity was significantly altered in both concentrations, by means of 5 and 15 µg L-1, when compared to control. This result can indicate an important role of PROD on the metabolism of E2 present in water. Regarding to the BROD activity, it could be observed differences statistically significant between control and both groups of treatments. Two or more CYP isoforms can contribute to the metabolism of the same compound, what makes BROD a candidate as a next bioindicator of the exposure to E2 in aquatic ecosystems. Analysis of variance could confirm the effect of E2 statistically significant on the GST activity in liver tissues with >90% of significance (Prob>F = 0.0753). Furthermore, it was possible to observe that the values of GSTs activities in liver and gills in both, control and treatments, follow a tendency, that means, enzymatic activity in gills increase as the increasing of the activity in the liver tissues. In this study, the 17β–estradiol was found in measurable concentrations in three sampled points, and these values were similar to the findings of other authors at different locations in Brazil. In addition, those values are much higher than the minimum concentration that presented observable effects (10 ng L-1).

Dopplerfluxometria e avaliação morfométrica do fígado, baço, rins e grandes vasos abdominais de onças pardas (Puma concolor) da região Campo Grande, Mato Grosso do Sul, Brasil

Pós-graduação em Biotecnologia Animal - FMVZ

Caracterização molecular de cryptosporidium spp. em filhotes caninos

Parasitos do gênero Cryptosporidium pertencem ao filo Apicomplexa, com localização intracelular e extracitoplasmática obrigatória e se desenvolvem principalmente na superfície das células epiteliais de hospedeiros vertebrados. O cão, possível fonte de infecção humana, elimina oocistos fecais deste protozoário com grande potencial zoonótico no ambiente. O presente estudo teve como objetivo caracterizar molecularmente Cryptosporidium spp. obtidos de amostras fecais de filhotes caninos (naturalmente infectados). Um total de 200 cães foram examinados, sendo 100 machos e 100 fêmeas, 111 de padrão racial determinado e 89 sem raça definida (SRD). Destes, 81 animais, 43, 48 e 28 tinham até dois, de dois a três; de três a seis e de seis a doze meses, respectivamente. Conforme sua origem, os animais eram provenientes dos Municípios de Araçatuba e Votuporanga, SP, sendo que 126 eram de domicílios; 11 mantidos em Centros de Zoonoses; 50 de Pet Shops; 12 de um criatório e uma (0,5%) era errante e havia sido adotada. A ocorrência de Cryptosporidium spp. foi de 1% (2/200). Ambas eram fêmeas, SRD, com idade entre 60 e 90 dias. A de origem residencial apresentava fezes pastosas com coloração castanho claro e a outra, resgatada do CCZ, material fecal escurecido de consistência liquefeita. O sequenciamento dos fragmentos amplificados confirmou a presença de Cryptosporidium canis. A partir dos resultados obtidos neste trabalho, é possível concluir que 2% dos caninos analisados eram hospedeiros de C. canis

Atividade de enzimas relacionadas ao processo de ossificação em girinos de Lithobates catesbeianus

Pós-graduação em Biotecnologia - IQ

Produção da nucleoproteína recombinante do vírus da influenza aviária para aplicação no imunodiagnóstico

Pós-graduação em Microbiologia Agropecuária - FCAV

Infecção de Lutzomyia longipalpis por leishmania spp. em área urbana endêmica para leishmaniose visceral no Estado de São Paulo, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Helminto fauna de anfíbios da família Leptodactylidae em três fisionomias de Cerrado

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Analise cladística - filogenética e paleobiogeográfica dos Mesoeucrocodylia (Crocodylomorpha; Crocodyliformes) do Grupo Bauru (Cretáceo Superior) do Sudeste do Brasil

Pós-graduação em Geociências e Meio Ambiente - IGCE

Obtenção e caracterização de nanofibras eletrofiadas via solução de quitosana com e sem inclusão de hidroxiapatita

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

GnRH/GnIH e seus receptores no sistema olfato-retinal de zebrafish

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diversidade cariotípica e molecular de Leptodactylus (Anura, Leptodactylidae) e obtenção de sondas cromossomo-específicas de anfíbio por citometria de fluxo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Physa mezzalirai: um novo gastrópode da formação Adamantina (Bacia Bauru), Cretáceo Superior, São Paulo, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A modernização da agricultura no Brasil e a produção do biocombustível como energia alternativa: uma reflexão crítica

This article discusses the policy implications of agricultural modernization implemented by the military - after 1964. This policy, which became known as the Green Revolution, on the one hand contributed to the development of big business, but another caused serious social and environmental impacts. Currently, not only in Brazil but all over the world, have been in a great debate about the need to find alternatives to contain the problems caused to the environment resulting from the use of high technology in the field. One alternative proposed by several researchers is to replace fossil fuels by biofuels. As we believe that the error is in the current model of agricultural production based on mass production, which serves the major markets, the attention in this article, the need to develop an agricultural model designed for small property, with the use of family work and agroecology.

Helmintofauna associada a leptodactylus fuscus (Anura: Leptodactylidae) em regiões de Cerrado, Pantanal e Caatinga no Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)