Estudo Comparativo de Três Diferentes Modalidades de Elisa para Medir os Anticorpos Contra o Vírus da Bronquite Infecciosa em Frangos Vacinados e Não Vacinados

No presente estudo, foram vacinados 150 frangos de corte de um dia de idade com sorotipo H120, e, após 28 dias desafiados à vacinação com o sorotipo M 41 do vírus da bronquite infecciosa das aves. da mesma forma, foram obtidos 150 soros de aves não vacinadas para a análise. Os respectivos soros foram analisados 28, 34 e 46 dias após o desafio, examinados através das técnicas de ELISA indireto (ELISA-I), Sandwich ELISA (S-ELISA) e ELISA com bloqueio de fase líquida (ELISA-BFL) e comparados com a técnica padrão de soroneutralização (SN) para efeito de cálculo da especificidade e sensibilidade relativas, bem como os valores predictivos positivos e negativos. O cálculo do coeficiente de correlação também foi empregado para a análise de concordância. Assim, os valores da correlação encontrados foram r = 0.98 entre ELISA-BFL x SNT, r = 0.79 entre S-ELISA x SNT e r = 0.74 ELISA-I x SNT. No entanto, quando comparamos as técnicas de ELISA entre si, ELISA-BFL x S-ELISA, ELISA-BFL x ELISA-I e S-ELISA x ELISA-I os valores encontrados foram r = 0.75, r = 0.69 e r = 0.79. A técnica de ELISA-BFL demonstrou melhor sensibilidade relativa que as técnicas de S-ELISA e ELISA-I, mesmo 46 dias após o desafio com a estirpe heteróloga. Entretanto, apesar das técnicas de S-ELISA e ELISA-I apresentarem especificidade realtiva superiores, a melhor correlação observada foi entre as técnicas de ELISA-BFL e a SN.

Desempenho Produtivo de Animais de Quatro Raças Zebuínas, Abatidos em Três Estádios de Maturidade. 1. Ganho de Peso e de Carcaça e Eficiência de Ganho

This research was conducted to evaluate the productive performance (live weight gain and carcass weight gain and gain efficiency) of animals from four zebu breed slaughtered in three maturity stages. Thirty-six bulls from Gyr, Guzera, Mocho Tabapua and Nellore with twenty-four months of average age and average initial live weight of 357.6, 362.0, 368.6 and 376.4 kg, respectively, were used. The bulls, from each breed, were randomly assigned to individual pens and were full-fed a diet containing 50% concentrate (DM basis) and distributed to three category (1, 2 and 3) of slaughter weights. The bulls from category 1, 2 and 3 were slaughtered when reached the individual live weight of 405, 450 and 500 kg, respectively. At slaughter the empty body weight was determined. The Nellore breed bulls showed greater average empty body weight gain and carcass gain in relation to the other breeds, which did not differ among themselves. There were no differences among breeds regarding to feed efficiency (empty body weight gain efficiency and carcass gain efficiency per unit of metabolizable energy intake). The slaughter weight did not influence the empty body weight gain and carcass gain, but animals slaughtered at higher slaughter weights showed lower gain efficiency and spent more time in feedlot.

Efeito da calagem sobre a produção de matéria seca de três gramíneas forrageiras

A greenhouse investigation was carried out to study the effects of liming on the dry matter (DM) production of the grasses Brachiaria brizantha (Hoscht.) Stapf. cv. Marandu, Andropogon gayanus Kunth cv. Planaltina and Panicum maximum Jacq cv. Aruana, cultivated on a Dark-Red Latosol, medium texture. The liming levels were obtained through the addition of carbonates in a Ca:Mg ratio of 4:1, necessary to increase the degree of base saturation (V%) to the following values; 4% (control), 20%, 36%, 52%, 68% and 84%. During the experimental period (180 days), four cuts were done to determine the DM production. The results indicated that the DM production was increased (P<0,05) with the liming-levels increased to the three grasses. Panicum maximum cv. Aruana yielded the lower DM quantity, but it presented a better response to liming. The inverse was observed in relation to A. gayanus. The data allowed to conclude that the DM production increased by liming and when the V% level was lower than 50%, it will be increased to 70% for establishment.

Influência do uso e ocupação do solo nos recursos hídricos do Córrego Três Barras, Marinópolis

Land occupation and its use have a remarkable influence on the superficial water flow and the carrying of sediments to the riverbed and may change the quality and availability of water. The current study aimed to check the influence of the land occupation and use on the Tres Barras stream resources, in Marinopolis, SP, Brazil. To check this influence, an analysis of Pearson's correlation between the parameters of quality and availability and use of water and land was developed. The samples covered the period of January, 2006 until December, 2007. It may be concluded that areas occupied by forests and pastures (in less intensity) favoured the availability and quality of water of the watershed. The inhabited areas and those used for agriculture, and the degraded forests in general, reduced the availability and quality of water in the watershed.

A Educação Musical Brasileira e Três Modelos Explicativos de Mundo: em busca de significados

In this paper we study the main modalities of music education in Brazil and how they can be compared to different views worldwide. Recognition that modern practices in music education are inserted in contemporary knowledge paradigms shows their relevance to a changing world. According to this contemporary paradigm the practices included herein point to alternatives in the relationship between men and themselves, men and other men and men and the environment. They also encourage cooperation, the relationship among the arts, the conduction of actions ruled by the principles of ecology and the recovery of values lost or weakened.

Influência da precipitação pluviométrica nas características físicas, química e higiênico-sanitária da água de trés mananciais de abastecimento público.

Aiming to know the influence of the pluviometric precipitation over the physical chemical and bacteriological characteristics of the water coming from three sources of public supply, and to verify the probable origin of faecal contamination, tests of total coliforms, faecal coliforms, faecal streptococci and mesophilic microorganisms, as well as the determination of pH, temperature and turbidity of 156 water samples were done. The samples were taken from a stream, a drain and an artesian well, located in the city of Jaboticabal, SP, Brazil. The results obtained showed that the occurrence of pluviometric precipitation determined a significant increase of the number of total coliforms in the water samples from the stream. The use of the ratio CF/EF, indicate the probable origin of the faecal contamination only in the samples of the water coming from the stream, which were defined as being of human origin. In relation to the physical-chemical composition it was noticed that the influence of the pluviometric precipitation over the turbidity appeared to be higher and significant in the samples of water of the stream.

Efeitos do potencial alelopático de três leguminosas forrageiras sobre três invasoras de pastagens

Bioassays were carried out to identify and characterize the allelopathic potential effects of forage legumes leucena (Leucaena leucocephala), mineirão (Stylosanthes guianensis cv. Mineirão) and calopogônio (Calopogonium mucunoides) on germination and radicle elongation of the pasture weeds desmódio (Desmodium adscendens), guanxuma (Sida rhombifolia) and assa-peixe (Vernonia polyanthes). Aqueous seeds and shoot extracts were prepared in a concentration of 10% (w/v). The pH and osmotic potential were measured in each extract. The effects of the osmotic potential on the results were calculated considering the regression equations adjusted to the variations of osmotic potential in each parameter and the osmotic potential of the extracts. The results showed that the pH did not constitute in a source of variation of the results. The donor species indicated allelopathic potential that varied in function of donor and receiver species and part of the donor plant. The aqueous shoot extract of mineirão and calopogônio showed inhibition potential higher than the extract from seeds, while for leucena the effects more evident were obtained with the extract from seeds. Comparatively, the radicle elongation was a more sensitive indicator than germination to the effects of the extracts.The receiver species assa-peixe was the less sensitive to the effects of the extract.

Épocas de consórcio de alho com beterraba perante três manejos do mato sobre a produtividade e o lucro

The research was carried out with intercropped cultivation of garlic and beet, in Caçador, SC, Brazil. Four seeding epochs for beet (0, 15, 30 and 45 days after garlic planting) and three management systems for weeds (with herbicides, without control and with frequent weeding) were tested. It was hoped to determine the effects of this intercropping, in time and space, seeking the benefits in .weed control, efficient land use, productivity, commercial quality, and profitability. The randomized block design was in split-plots. The epochs represented the plots, and the management, the subplots, with four replicates. Napromide at 750 g/ha did not cause symptoms of intoxication in the garlic plants, nor in the beet. All the treatments in epochs at the initiation of intercropping, in three management systems for weeds, presented efficient land use values greater than 1. The profitability of the intercropping of garlic and beet only became evident in the management involving frequent weeding; in all epochs, profits were greater than for the respective epochs of monoculture of beet and monoculture of garlic.

Propriedades físicas e infiltração de água de um Latossolo Vermelho Amarelo (Oxisol) do noroeste do estado de São Paulo, Brasil, sob três condições de uso e manejo

This study presents the results obtained in a field experiment carried out at Glicério, Northwest of São Paulo state, Brazil, whose objective was to analyze changes of selected soil physical properties and water infiltration rates on a Yellow-Red Latosol, under three different management conditions. The experimental design was arranged as completely randomized split-block with twelve treatments, which corresponded to four depths (0-0.05 m; 0.05-0.10 m; 0.10-0.20 m and 0.20-0.40 m) and three conditions of soil use and management with four replications. The soil surface conditions were: conventional tillage (one disking with moulboard plus two levelling passes with harrow), nine months before starting filed experiences; recent conventional tillage (also one disking with moulboard plus two levelling passes with harrow) and native forest. The conventional tillage areas were cropped for about fifteen years with annual cultures. The considered soil general physical properties were: macroporosity, microporosity, total porosity, bulk density, soil moisture and penetration resistance and, in addition; soil water infiltration rates were also recorded. According to our results, differences on general soil physical properties and infiltration rates appeared when both tilled sub-treatments and native forest were compared. Both, plots recently prepared by conventional tillage and those prepared by tillage but left nine months in rest, presented a statistically significant decrease of constant (final) water infiltration rates of 92.72% and 91.91% when compared with native forest plots.

Desenvolvimento de três cultivares de mangueira sobre quatro porta-enxertos

The experiment was conducted at the physics structure of the Rio Grande do Norte State Farming Research Company (EMPARN) in Açu-RN, with the objective of evaluate the behavior of grafts-holder cultivates in the plants formation of cultivates used as tops. The experimental design was randonizated blocks in the factorial outline 3 x 4, with 3 repetitions. The analyzed factors were four cultivates of grafts-holder and three cultivates of grafts. As grafts-holders were used the cultivates Carabao and Manga d'agua, originated at the Campinas Agrarian Institute, and the cultivates Rosinha and Espada, obtained from the EMPARN collection. The cultivates used as tops were Tommy Atkins, Van Dyke and Keitt, with the fork that were also obtained at EMPARN. In a period of six months was observed the diameters of the grafts-holder, grafts and hight of the plants. The Cultivar Manga d'agua, as a graft-holder, provided the smallest growing to the mango tree and the Cultivar Tommy Atkins, used as graft, was the one who presented the tallest hight.

Espectro de agentes etiológicos associados a surtos de doenças transmitidas por alimentos em núcleos receptores turísticos de três regiões geográficas do Estado de São Paulo

Foodborne and waterborne diseases are spread by the consumption of food or water contaminated with bacteria and/or their toxins, viruses, parasites or chemicals. The aim of the research reported here was to establish the spectrum of etiologic agents of foodborne outbreaks at 15 tourist resorts in three geographic regions of the State of Sao Paulo (Brazil). The study was based on the cases reported to the Epidemic Surveillance Center (CVE) of the Public Health Authority of the State of São Paulo (SES), from 2002 to 2005. The tourist centers were chosen at random in three regions of the state (Capital, Interior and Coast) and offered the following attractions: events, agribusiness, cultural history, shopping, town center, gastronomy, health and leisure, sun and sea. Among the bacteria, the results showed that Salmonella spp. were most frequently associated with outbreaks, followed by Shigella spp., enterotoxigenic Escherichia coli, Staphylococcus aureus, Clostridium perfringens, Bacillus cereus and Campylobacter spp. Viruses (Rotavirus and Hepatitis A) played a part in many of the cases, while the frequency of parasites and worm infestations was low in the foodborne disease outbreaks at these resorts. The mixed foods (rice, beans, liver, potatoes, barbecue, juice), fish and poultry were the three commonest vehicles implicated in the outbreaks.

Aspirado traqueal de cavalos clinicamente sadios da raça quarto de milha após prova de três tambores

The aim of the present study was to evaluate through endoscopy the tracheal aspiration cytology in twenty seven Quarter Horses from Curitiba and surroundings, following the Three Barrel Competittion. Upper respiratory tract secretion was obtained by tracheal aspiration using a polyethylene catheter introduced through the endoscopic fiberoptic working channel, at the level of tracheal bifurcation. Cytologic slides were prepared by smear and stained by diff-quick technique and the differential was performed in 500 cells counting by 1,000X optic microscopy. None of the horses presented abnormality, including epixtasis, at the clinical examination. However, hemosiderophages were detected at cytology in three animals, suggesting that some may be suffering of subclinical pulmonary hemorrhage. Differential cell counting of tracheal aspiration results were, in average: 44.09 ± 35.68% of epithelial cells; 1.10 ± 2.18% of Globet cells; 23.10 ± 35.93% of neutrophils; 0.13 ± 0.37% of lymphocytes; 0.91 ± 2.81% of eosinophils; 30.57 ± 23.62% of macrophages and 0.13 + 0.93% of hemosiderophages. In conclusion, based in the present study, the evaluation of cellular populations with the tracheal aspiration may offer important additional information to the clinician, particularly about the inflammatory processes of lower respiratory tract and pulmonary bleeding.