Estudo comparativo entre anestesia venosa total alvo-controlada e por infusão contínua em cães pré-tratados com levomepromazina e tratados com propofol e remifentanila

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Síntese de cerâmicas nanoestruturadas híbridas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e eficiência de isolados de Metarhizium anisopliae (Metsch.) Sorok. no controle da cigarrinha-das-raízes da cana-de-açúcar, Mahanarva fimbriolata (Stal, 1854) (Hemiptera: Cercopidae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Análise da prevalência e de fatores de risco para as hepatites virais crônicas B e C em idosos residentes no município de Botucatu-SP

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Efeitos do calor e da ação microbiana em filmes de PHBV / PP-co-PE aditivados em solo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Perfil epidemiológico de acidentes com material biológico entre estudantes de medicina em um pronto-socorro cirúrgico

OBJECTIVE: To evaluate the accidents with biological material among medical students interning in a trauma emergency room and identify key related situations, attributed causes and prevention. METHODS: we conducted a study with a quantitative approach. Data were collected through a questionnaire applied via internet, with closed, multiple-choice questions regarding accidents with biological material. The sample comprised 100 students. RESULTS: thirty-two had accidents with biological material. Higher-risk activities were local anesthesia (39.47%), suture (18.42%) and needle recapping (15.79%). The main routes of exposure to biological material were the eyes or mucosa, with 34%, and syringe needle puncture, with 45%. After contamination, only 52% reported the accident to the responsible department. CONCLUSION: The main causes of accidents and routes of exposure found may be attributed to several factors, such as lack of training and failure to use personal protective equipment. Educational and preventive actions are extremely important to reduce the incidence of accidents with biological materials and improve the conduct of post-exposure. It is important to understand the main causes attributed and situations related, so as general and effective measures can be applied.

1,3-propanodiol: produção, aplicações e potencial biotecnológico

1,3-propanediol is a high-value specialty chemical which has many industrial applications. Its main use is the production of the polymer polypropylene terephthalate, a thermoplastic used in the textile and automobile industries. The interest in 1,3-propanediol production from glycerol bio-conversion has increased after the employment of biodiesel by various countries, being produced by chemical synthesis from petroleum intermediates or biotechnologically by microbial fermentation. Glycerol is an abundant low-cost byproduct from biodiesel refineries, and it is the only substrate that can be naturally or enzymatically converted to 1,3-propanediol by microbial fermentation. In this review, information on 1,3-propanediol's importance, production and purification are presented, along with results from recent research on glycerol microbial conversion to 1,3-propanediol. The bio-production of this intermediate compound from glycerol is very attractive both economically and environmentally, since it allows the replacement of fossil fuels by renewable resources.

Produção de beterraba em função do espaçamento

Atualmente alguns híbridos importados têm substituído as tradicionais cultivares de beterraba de mesa, sem no entanto, desenvolver estudos básicos sobre a melhor densidade de plantio para as condições brasileiras. O objetivo deste trabalho foi avaliar o efeito do espaçamento entre linhas e entre plantas na produção de beterraba híbrido Boro. O experimento foi conduzido em campo da UNESP em Botucatu-SP, de 10 de setembro a 8 de dezembro de 2010. Foram estudados dois fatores, espaçamentos entre linhas (EL) de 20 e 25 cm, e 7,5; 10,0 e 12,5 cm entre plantas (EP), no esquema fatorial 2 EL x 3 EP, totalizando seis tratamentos (populações variando de 246.400 a 513.333 plantas ha-1, considerando-se canteiros com 1,0 m de largura e espaço entre canteiros de 0,3 m). O delineamento experimental foi em blocos ao acaso, com cinco repetições e parcelas de 2 m2. As mudas foram produzidas em bandejas de polipropileno com 288 células. A irrigação foi por aspersão e a adubação foi igual para todos os tratamentos, seguindo a recomendação para o estado de São Paulo. A maior massa fresca por raiz (116,1 g) foi obtida no EL de 25,0 cm. Em relação ao comprimento da raiz, observaram-se diferenças significativas apenas para o EP, sendo que o maior espaçamento, 12,5 cm, resultou em maior comprimento (63,1 mm). Foram obtidos maiores valores de diâmetro das raízes quanto maiores os EP (65,6 mm para 12,5 cm) e EL (63,1 mm para 25 cm). A maior produtividade (44,7 t ha-1) foi obtida no menor EP (7,5 cm), enquanto que o EL não afetou a produtividade. Pode-se concluir que o melhor EP foi de 7,5 cm pela maior produtividade. Quanto ao tamanho da raiz, tanto o maior EL (25 cm) como o maior EP (12,5 cm) resultaram em raízes com maiores massa média e diâmetro.

Free radical entrapment and crystallinity of resin composites after accelerated aging as a function of the expiration date

This study evaluated the spin concentration and the crystallinity in different classifications of dental composites as a function of the material condition (new, aged and expired). Specimens were obtained according to the factors: composites: Filtek P60, Filtek Z250, Filtek Z350XT, and Filtek Silorane; and material conditions: new, aged, and expired. The syringe composites underwent an accelerated aging protocol (Arrhenius model). The magnetic properties of the composites were characterized using Electron Paramagnetic Resonance (EPR) and the concentration of spins (number of spins/mass) was calculated. The crystallinity of the composites tested was characterized with X-ray diffraction (XRD). Filtek P60 and Filtek Z250 presented similarities in terms of spin concentration and crystallinity, irrespective of the material condition. The aging protocol influenced the composite Filmic Z350XT that exhibited a significant increase in the spin concentration. Besides, lower intensity peaks of the organic matrix and amorphous silica were also observed for both aged and expired Filtek Z350XT. Although a significant lower spin concentration was observed for the silorane composite in comparison to that of the methacrylates, a decrease in the relative intensity of peaks of the amorphous region related to the organic components in the diffractograms was observed. The material conditions tested influence the crystallinity and the magnetic properties of the composites evaluated. (C) 2014 Elsevier Ltd. All rights reserved.

Resin composite characterizations following a simplified protocol of accelerated aging as a function of the expiration date

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Bone Marrow'S Harvest in The Coxal Tuberosity for Isolation and Culture of Mesenchymal Stem Cells of Buffaloes (Bubalus Bubalis)

Several studies with mesenchymal stem cells (MSCs) have been developed in many species because of its ability to differentiate into other mesoderm lineages, capacity of self-regeneration, low immunogenicity, paracrine, anti-inflamatory, immunomodulatory and antiapoptotic effects which make then a promissory source to be used in therapeutic strategies. The aim of this study is to report the technique of harvest of bone marrow (BM) in the coxal tuberosity (CT) of buffaloes. For this, the animals were selected, identified and contained in a stock. Then trichotomy was performed in the region corresponding to the CT. After identifying the anatomic site it was performed antisepsis, local anesthetic block and introduction of a myelogram's needle (Lang(R)) for BM aspiration. Once the needle was firmly fixed in the CT, the mandril was removed and proceeded to BM aspiration with a syringe (20 mL) containing 1 ml of heparin at 1000 IU / mL and 1 mL of PBS. After the collection, each sample collected was manually homogenized, identified and referred to the LRACT - FMVZ / UNESP-BRAZIL for the correct processing. The anatomical site tested showed to be an alternative site of harvest of BM once provided the appropriate isolation and culture of the mononuclear fraction. Moreover, the procedure was performed without difficulty and with great security. Based on this, it can be conclude that CT is an excellent anatomical site for isolation and culture of MSCs and the proposed technique is viable and feasible to be held in buffaloes.

Isolation, Culture and Differentiation of Buffaloes Bone Marrow Mesenchymal Stem Cells Obtained from the Coxal Tuberosity

Currently, much attention has been devoted to the renewal of knowledge about Stem Cells and Cell Therapy in domestic species. In this sense, the present work aimed to develop a methodology for collecting, processing and cultivation of mesenchymal stem cells obtained from bone marrow of coxal tuberosity in buffaloes. The collection was performed using a Komiyashiki needle, which was introduced in the coxal tuberosity and the bone marrow aspirated into a heparinized syringe with the aid of negative pressure. Directly after collection samples were processed at the laboratory at FMVZ - UNESP. The samples took approximately 32 days to reach 80% confluence, when the first passage and differentiation was performed. To confirm the mesenchymal origin, cells were induced to differentiate into adipogenic and osteogenic lineages. Samples showed morphological changes during differentiation protocol, but not all presented production of extracellular deposits of calcium or intracellular fat droplets, observed after staining with Alizarin Red and Oil Red respectively. Compared with the material obtained from other species and processed in the same laboratory, the primary culture was longer. Therefore, more studies are needed to standardize the age of animals used and to test other inducers of cell differentiation.

Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate

Zymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 10(7) CFU mL(-1) by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30 degrees C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 10(9) CFU mL(-1) during fermentation time.

Conservação pós-colheita de melões inteiros e minimamente processados

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Effects of autohemotherapy on hematological responses in Wistar female rats Autohemotherapy in rats

Introduction: Autohemotherapy is a type of treatment that have acquired an opposite role and have presented its efficiency strived by the medical community for many reasons. In this study we aimed to evaluate the effects of authohaemotherapy on hematological response. Method: We used Wistar female rats (300g). The study consisted in a control group and a treatment group, blood samples were collected at the first day and at the eighth day after the application. In the both groups we collected 300 μl of blood from each rat through a syringe with a previously prepared solution of 30 μl of sodium citrate 3.2%. In the autohemotherapy group the blood sample was immediately injected in the quadriceps muscle on the back of the thigh hind limb. Rats from the control group did not receive intramuscular blood application. The cellular count was done through flow cytometry and the samples were dosed for immunoglobulin. Results: In the both groups we observed increased production of erytrocites, hemoglobin and platelet (p<0.05). However, there was reduction of basophil in the control group and reduction of lymphocyte, monocyte and neutrophil in the both groups. No effects were observed in IgA, IgG and IgM levels. Conclusion: Autohemotherapy did not influence hematological responses in Wistar female rats.