Lipoperoxidação, perda de viabilidade celular e diminuição da liberação de IL-8 de neutrófilos humanos na presença de corpos cetônicos

Type-1 diabetes patients suffer from frequent episodes of acidosis caused by an increased fatty acid metabolism and consequently increased plasma level of acetoacetate (AcAc) and β-hydroxybutyrate (β-HOB). This article describes a study of the effects of pathological concentrations of AcAc and β-HOB on lipoperoxidation, cell viability and the release of the CXCL8 (IL-8) cytokine by activated neutrophils. Neutrophils from healthy donors were isolated by density gradient (Histopaque® 1077/1119) and incubated with the ketone bodies. Lipoperoxidation was determined as thiobarbituric acid reactive substances (TBARS). The cell viability was evaluated by the release of intracellular lactate dehydrogenase. The release of CXCL8 was measured by ELISA in a 24-h culture of opsonized zymosan-stimulated neutrophils. AcAc, but not β-HOB, provoked a dose-dependent increase in the neutrophil membrane lipoperoxidation (p<0.05; r =0.9915). In the cytotoxicity assay, a dose-dependent release of LDH was observed when the neutrophils were incubated with AcAc in concentrations up to 40 mM (p<0.05). β-HOB was devoid of effect. The release of CXCL8 was inhibited by AcAc and β-HOB in a dose-dependent manner. In conclusion, these results suggest that the accumulation of ketone bodies in diabetic patients could be involved in their usually increased susceptibility to infection.

Viabilidade econômica e seleção de sistemas de irrigação utilizando simulação e dominância estocástica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evolução da perda de peso entre indivíduos da fila de espera para a cirurgia bariátrica em um ambulatório multidisciplinar de atenção secundária à saúde

Objective: The objective of this study is to characterize the assistance and analyze patient's adherence to nutritional follow-up while in the waiting line for bariatric surgery in a multidisciplinary, secondary healthcare outpatient clinic. Methods: This retrospective study was based on the nutritional records of 59 patients subjected to bariatric surgery at the Hospital das Clinics de Botucatu, from 2001 to 2008. The population studied was distributed into 8 groups and analyzed according to follow-up duration. The analysis took into account the evolution of body weight and the number of follow-up visits in a given period. The Kruskal-Wallis test was used to compare data between groups while Spearman's coefficient was used to test correlations. Results: Among the 59 studied patients, 42 (71.2%) returned to the clinic at least once in six months. Of these, 67.8% lost weight while in the waiting line and 27.1% of them lost more than 10% of their body weight. The greatest weight losses in the preoperative period were found in the groups that had more than six months of nutrition follow-up. There was a mean delay of three months between return visits, which could have contributed to the low adherence to follow-up. Conclusion: The patients in the waiting line for surgery analyzed in this study presented low adherence to the long-spaced return visits. The greatest weight losses were observed in groups that had been followed for more than six months. This shows that, to achieve the desired weight loss and an adequate education process before surgery, at least six months of follow-up and regular visits are necessary.

Viabilidad económica de la utilización de biogás para la conversión en energía eléctrica

The economic viability of the electrical power generation using biogas from swine wastes, was determined. The analyzed biodigester is a continuous tubular model with brick concrete duct and plastic covering with a gasometer, in which the waste of 2.300 fattening pigs are deposited daily. The initial investment estimate for the installation was R$ 51.537, the system annual costs were R$ 5.700, for maintenance, R$ 4.400 for depreciation and R$ 1.370 for interests. It was found that an average consumption of 28 kW-hour-1 is the minimum that the system must reach to be economically feasible.

Perda de carga em mangueiras gotejadoras novas e usadas

This paper aims to determine the importance of emitter ́s local head loss in the hydraulic design of hose drip lines, as well as comparing the result of equations that calculate the local head loss; besides verifying the influence of iron precipitated at the hose head loss made in Brazil. The result found that localized head loss represents 23% of the total head loss; the equations showed significant results and the total head loss increased in an average of 20% after the irrigation system was used with water rich in iron.

Viabilidade econômica de quatro intensidades de pastejo do capim Tanzânia na pecuária de corte1

Were evaluated the economic effects of four grazing heights (20, 40, 60 and 80 cm) of Tanzânia grass for beef cattle. The total area was 12 acres divided into paddocks of one hectare, with three replications. We used three animals, males, Nelore per paddock, as more animals need additional adjustment to the height you want. The reduction in sward height allowed higher stocking rate, which, even with a reduction in individual performance, there was more beef production per area. The interest rate on capital given the property represented the largest share in the final cost of production. The participation of fixed variables showed a positive linear function of the increase in height from grazing, showing significant reduction of the production scale. A reduction in cost of production per hectare per year with increasing grazing height. No differences were found in certain economic indicators, and the four systems remunerate the capital invested.

Custos e viabilidade econômica da produção de casulos do bicho-da-seda

Sericiculture is characterized by the possibility of income generation in areas smaller than 10.0 ha and by the rationalization of family labor. Even considering this favorable scenario for the development of this activity, there is still a gap in the management of its economic factors in most of the rural properties within this chain, contributing negatively to their expansion. This study aimed at assessing the production cost and economic viability for producing silkworm cocoons, by using a case study conducted in a property located in Quatá, São Paulo State, Brazil. The main results for a production of 1,904 kg-1 of silkworm cocoons, with labor costs reaching 51.5% of the Effective Operating Cost and 35.5% of the Total Operating Cost, were a profitability index higher than 50% and an Internal Rate of Return of 9.68%, for the second production year, providing a good profitability rate for this agricultural segment, when we consider that the improved profitability of the producer is proportional to the best production indicators, particularly due to the fast return that this activity provides.