Influência da fixação e de métodos de secagem na detecção de alterações proliferativas ultraestruturais induzidas pelo Diuron no urotélio vesical de ratos wistar

Pós-graduação em Patologia - FMB

Imobilização de lipases por adsorção e ligação covalente em derivados de agarose e quitosana e sua aplicação em biocatálise

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Scanning Electron Microscopy of the Tongue, Pharynx, and Larynx of Rats Exposed to Cigarette Smoke

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Stabilization of dentin matrix after cross-linking treatments, in vitro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Uso de invertase imobilizada em pó de sabugo de milho para produção de açúcar invertido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e imobilização de celulases em matriz de agarose com diferentes ativações químicas

Pós-graduação em Biotecnologia - IQ

Ti/TiO2 nanotubes enhance Mycobacterium fortuitum, Mycobacterium chelonae and Mycobacterium abscessus inactivation in water

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Trichodina heterodentata (Ciliophora) infestation on Prochilodus lineatus larvae: a host-parasite relationship study

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Síntese de microesferas de quitosana para aplicação em liberação controlada de drogas pelo método de atomização e coagulação

Chitosan is a natural biodegradable polymer with great potential for pharmaceutical applications due to its biocompatibility, high charge density , nontoxicity and mucoadhesion. Gel formation can be obtained by the interactions of chitosans with low molecular counterions such as polyphosphates, sulphates and crosslinking with glutaraldehyde. This gelling property of chitosan allows a wide range of applications such as coating of pharmaceuticals and food products, gel entrapment of biochemicals, whole cells, microorganisms and algae. One of its main applications is the synthesis of microspheres for coating of pharmaceuticals , magnetic particles an other substances. In such a way, we can build targeted drug delivery systems. In the present work, we applied the method of spraying and coagulation. The resulting microspheres, then, were characterized by optical microscopy

Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento

Pós-graduação em Cirurgia Veterinária - FCAV

Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering

Scaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities.

Obtention and characterization of collagen and chitosan based cements for bone regerneration. Part 1: extraction and characterization of collagen

Several cements are used as biomaterials. Biopolymers such as chitosan and collagen exhibit excellent biocompatibility and can be used in the remodeling of bone tissue. The cement must have high mechanical strength and compatibility with original tissue. In this context, the objective of this study was to extract, characterize and cross-link collagen from bovine tendon, forlater associate it with chitosan and calcium phosphate to obtain cements for bone regeneration. Glutaraldehyde was used as cross-linker in 0.1, 0.5, 1.0 and 10% concentration. Infrared analysis confirmed the presence of functional groups characteristic of collagen, whereas the capacity of water absorption decreased with the increasing of cross-linking degree. Denaturation temperatures of collagen samples were obtained by Differential Scanning Calorimetry and Scanning Electron Microscopy showed the fiber structure characteristics of collagen, which were more organized for high degree of cross-linking samples.

Reuse of Cyclodextrin Glycosyltransferase through Immobilization on Magnetic Carriers

National Council for Scientific and Technological Development (CNPq)

Immobilization of papain on chitin and chitosan and recycling of soluble enzyme for deflocculation of saccharomyces cerevisiae from bioethanol distilleries

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Matrizes de quitosina-colágeno podem regular as atividades biológicas de células tronco mensenquimais adiposas para a engenharia de tecidos

Pós-graduação em Biofísica Molecular - IBILCE