Healing of fresh frozen bone allograft with or without platelet-rich plasma: A histologic and histometric study in rats

Purpose: This study histomorphometrically analyzed the effect of autogenous platelet-rich plasma (PRP) on healing of fresh frozen bone allograft (FFBA) in bony defects in rat calvaria. Materials and Methods: A 5mm-diameter defect was created in the calvarium of 30 rats. Animals were divided into three groups: C (defect was filled by blood clot only), FFBA (defect was filled with 0.01mL of FFBA), and FFBA/PRP (defect was filled with 0.01mL of FFBA combined with 100μL of PRP). All animals were euthanized at 30 days postoperatively. Histomorphometry and histology analyses were performed. Data were statistically analyzed (analysis of variance, Tukey, p<.05). Results: FFBA had a statistically smaller new bone area than groups FFBA/PRP and C. No statistically significant differences were observed between groups FFBA and FFBA/PRP with regard to remaining bone graft particle area. Conclusion: It can be concluded that (1) PRP improved the incorporation of FFBA, increasing the amount of new bone formed; (2) PRP has not influenced the resorption of nonviable particles of the FFBA; and (3) presence of remaining FFBA particles might have accounted for the smaller amount of new bone observed in group FFBA when compared with control group. © 2011 Wiley Periodicals, Inc.

Quantification of methomyl levels in cabbage, tomato, and soya milk using a renewable amperometric biosensor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Clinical similarities and histological diversity comparing fresh frozen onlay bone blocks allografts and autografts in human maxillary reconstruction

Background: In the absence of autologous bone for harvesting, fresh-frozen bone allografts turned into an alternative for bone reconstruction procedures. Purpose: The purpose of this study was to make a histological analysis of fresh-frozen onlay bone allografts (ALs), compared with autografts, in patients who needed maxillary reconstruction prior to dental implants placement. Materials and Methods: Twelve patients with bone deficiencies (width inferior to 4mm) in the sites where the implants were planned were enrolled in the study. From these, six were elected to be treated with autogenous (AT) bone grafts and six with fresh-frozen bone AL. This last group included the patients who had absence of a convenient amount of bone in donor sites. Each patient received from one to six graft blocks, totalling to 12 ATs and 17 ALs. Seven months after grafting procedures, biopsies of the grafts were made using 2-mm internal diameter trephine burs, and processed for histological analysis. One biopsy was retrieved from each patient. Results: Clinically, all grafts were found to be firm in consistency and well-incorporated to the receptor bed. Histological analysis showed a large amount of necrotic bone surrounded by few spots of new-formed bone in the AL group, suggesting low rate of graft remodeling. In the AT group, an advanced stage of bone remodeling was seen. Conclusions: Human fresh-frozen bone block AL showed clinical compatibility for grafting procedures, although associated to slow remodeling process. Further studies are needed to define, at long term, the remodeling process chronology the clinical longitudinal results for fresh-frozen bone AL. Copyright © 2013 Wiley Periodicals, Inc.

Immunological aspects of fresh-frozen allogeneic bone grafting for lateral ridge augmentation

Objectives: To present some immunological aspects of fresh-frozen allogeneic bone grafting for lateral bone augmentation, based on the quantitative evaluation of IL-10, IL-1β, IFN- γ and TNF- α in patients sera. Material and methods: Thirty-three partially or totally edentulous patients received fresh-frozen allogeneic bone (AL - 20 patients) or autologous bone onlay block grafts (AT - 13 patients) prior to oral implant placement. Blood samples were collected from each patient at various time-points during a 6 month-period (baseline, 14, 30, 90 and 180 days postoperatively). Quantitative evaluation of IL-10, IL-1β, IFN- γ and TNF- α was performed by enzyme linked immunosorbent assay (ELISA). Results: For all evaluated markers and at all evaluated periods, inter-group comparisons showed no statistically significant differences between the groups, while the observed values were within normal levels. For AL-treated patients, intra-group evaluation showed statistically significant increase of TNF-α from baseline to 90 (P < 0.001) and 180 (P < 0.01) days, and from 14 to 90 (P < 0.01) and 180 (P < 0.05) days. IFN- γ showed intercalated results, with a decrease from baseline to 14 days (P < 0.05), and increase from 14 to 90 days (P < 0.001) and 180 (P < 0.05) days. No differences between the periods of evaluation were found for the AT group. Conclusions: AL grafting for lateral bone augmentation, similar to AT grafting, does not seem to challenge the immune system significantly. © 2012 John Wiley & Sons A/S.

Leaf senescence in tomato mutants as affected by irradiance and phytohormones

We explored the interaction between radiation of different wavelength and jasmonic acid (JA) or brassinosteroids (BR) on leaf senescence-induced oxidative stress. Three approaches were used: 1) jasmonic acid insensitive1-1 (jai1-1) and brassinosteroid-deficient [dumpy (dpy)] mutants were treated with red (R) or far-red (FR) radiation; 2) phytochromedeficient aurea (au) and high pigment-1 (hp-1) (radiation exaggerated response) mutants were treated with methyl jasmonate (MeJA) or epibrassinolide (epiBL); and 3) double mutants au jai1-1 and au dpy were produced. Leaf chlorophyll content, lipid peroxidation, and antioxidant enzyme activities were determined. After senescence induction in detached leaves, we verified that the patterns of chlorophyll degradation of hormonal and photomorphogenic mutants were not significantly different in comparison with original cv. Micro-Tom (MT). Moreover, there was no significant change in lipid peroxidation measured as malondialdehyde (MDA) production, as well as catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) activities in the hormonal mutants. Exogenous BR increased CAT and APX activities in MT, au, and hp-1. As concerns the double mutants, severe reduction in H2O2 production which was not accompanied by changes in MDA content, and CAT and APX activities was observed during senescence in au dpy. The results suggest that JA and BR do not participate in light signaling pathway during leaf senescence-induced oxidative stress. © 2013 Springer Science+Business Media Dordrecht.

Uso do extrato pirolenhoso: Efeito no solo e nas plantas de tomate

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de fungicida via irrigação por gotejamento na cultura do tomateiro em cultivo protegido

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Desenvolvimento do tomate em diferentes níveis de irrigação e de doses de salinidade

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Interferência relativa entre o tomateiro industriale Amaranthus viridis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ocorrência de begomovírus de plantas de pimentão no Estado de São Paulo e comportamento de genótipos de Capsicum spp. ao tomato severe rugose virus e a Bemisia tabaci meam1

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Aplicação de luz na faixa do vermelho-extremo em mudas e diferentes sistemas de condução do tomateiro cultivado em ambiente protegido

Pós-graduação em Agronomia - FEIS

Interação de Tomato severe rugose virus com Bemisia tabaci biótipo B, a acessos de Capsicum spp. e ocorrência de espécies de mosca-branca no Estado de São Paulo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito do processo de obtenção do catchup sobre seus compostos antioxidantes, capacidade seqüestrante do radical DPPH e cor

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de Fungicida Na Cultura do Tomateiro Via Água de Irrigação em Cultivo Protegido

O presente trabalho objetivou estudar os efeitos de uma nova técnica de aplicação de fungicida, através da água de irrigação (gotejamento), na cultura do tomate híbrido “Débora Plus”, no controle da doença causada pelo fungo Septoria lycopersici Sepg. Além de estudar a comparação dos tipos de aplicação, foi estudado também 6 (seis) doses do fungicida benomyl na fungigação. As doses utilizadas foram: 75% (59,5 mg/vaso), 50% (119,0 mg/vaso), 25% a menos da dose recomendada (178,5 mg/vaso), 100% da dose recomendada (238,0 mg/vaso), 25% (297,5 mg/vaso) e 50% superior à dose recomendada (357,0 mg/vaso). As doses utilizadas nas aplicações via pulverização foram de 100% da dose recomendada pelo fabricante (238,0 mg/vaso). Para efeito de comparação entre os tratamentos avaliaram-se o número e pesos das matérias fresca e seca dos frutos colhidos e a altura das plantas. Na instalação do experimento adotou-se o delineamento em blocos casualizados com oito tratamentos e cinco repetições. De acordo com os resultados obtidos verificou-se que a aplicação convencional do benomyl, para controle da septoriose do tomateiro, foi superior à fungigação. Não houve diferença significativa entre as diferentes dosagens do produto na fungigação, mas houve o controle da doença. O parâmetro altura das plantas foi semelhante para todos os tratamentos, evidenciando que tanto a fungigação como a pulverização convencional não interferiu no crescimento das mesmas. O cultivo das plantas em casa de vegetação, utilizando vasos, proporcionou excelente resultado no controle de pragas e doenças, pois os tomateiros não apresentaram pragas tampouco outras doenças, além da Septoria lycopersici, que foi inoculada. Mesmo nas plantas testemunhas não houve disseminação da septoriose e, a irrigação com garrafas individuais também indicou bons resultados, pois deste modo sabe-se a quantidade de exata de fertilizantes, defensivos e água aplicada na planta.