OLEO ESSENCIAL EM ALGUMAS ESPECIES DE EUCALYPTUS

In this work we used leaves of four species of Eucalyptus - E. bicostata Maiden, E. deanei Maiden, E. dunnii Maiden and E. viminalis Labill, grown in Colombo, Parana State, Brazil. Preliminary studies were done, in the following sequence: botanic identification, olfactory research, extraction and purification of the essential oil of each species. The physical and chemical tests done were the following: relative density, refractive index, optical rotation, solubility, coagulation point and cineol percentage. The essential oils analysed through gaseous chromatography, presented the following cineol percentage: E. bicostata Maiden - 26.92%, E. deanei Maiden - 70.21%, E. dunnii Maiden - 53.16% and E. viminalis Labill - 23.05%.

Sensitivity of mycobacteria to different species of eucalyptus l'herit

Sensitivity profile of 10 mycobacteria of clinical interest to eight different species of Eucalyptus L'Herit was studied. A Proportion Method Indirect Test was performed to detect resistant bacilli utilizing 5 and 10 mg/ml of essential oils incorporated to Lowestein-Jensen medium. Mycobaterium tuberculosis and other slow growing mycobacteria were more sensitive to essential oils than faster growing ones. The most effective essential oils, in decreasing order, were Eucalyptus citriodora, Eucalyptus maculata and Eucalyptus tereticornis.

Níveis críticos de fósforo no solo e nas folhas para a implantação de Eucalyptus grandis Hill ex Maiden, em quatro tipos de solos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Variação da massa específica da madeira de Eucalyptus grandis aos 8 anos de idade em função de diferentes níveis de produtividade

It were analyzed Eucalyptus grandis trees of 8 years old in the forest group of Duraflora S/A, with different annual increments based on volume, to the related studies of specific gravity values based on productivity indexes. The methodology used in analysis of specific gravity was the gamma-ray attenuation technique of Americium-241. The Eucalyptus grandis showed a specific gravity decreasing with the increasing of parcel productivity. For a gain of 3,5 m3 in productivity there was a decreasing of 1% in specific gravity. Nevertheless, it was not observed correlation between specific gravity and basal area in a same parcel. The present work stands out too, some of the difficulties found with relation to wood specific gravity determinations and emphasize the importance of take samples in the form of full rounded plates in three or four relative positions in the tree.

Manejo de brachiaria decumbens e seu reflexo no desenvolvimento de Eucalyptus grandis

A field trial was carried out in Santa Rita do Passa Quatro, SP, on sandy soil, between February 1993 to February 1995, aiming to study the effects of fertilizer doses and brachiaria grass control on the growth of E. grandis. In the field the plots (9×9 plants, spacing 2×3 m) were located following a randomized block experimental design with four repetitions. The treatments were arranged in a factorial design with four systems of brachiaria grass control in the space between the Eucalyptus rows: mowing, cultivation, chemical control with glyphosate (2,08 kg eq. ác./ha) and hand hoeing were developed when the population reached the early flowering stage and four doses of the fertilizer 20-05-20: 0, 115, 230 and 345 kg/ha, handled at 3, 6 and 12 monthes after the transplantation. The hand hoeing was the most effective method of brachiaria grass control. However the hand hoeing controlled plots showed a decrease on the E. grandis growth exhibiting slower growth rate, shorter plants, thinner stems, smaller leaf area results and reduction on dry matter accumulation than the plants from the other plots under different weed control management's. The glyphosate promoted an excellent brachiaria grass control while the E. grandis plants grow better. Both remainder weed control management systems were intermediary in terms of efficacy. The mowing management was the most similar method as compared to the hand hoeing one and the cultivation treatments to the chemical control method. The growth rate differences observed between the hand hoeing and chemical control treatments were not caused directly by late fertilizations. There were no significative interactions among the weed control systems and the fertilization doses. Considering the brachiaria grass that grew between the E. grandis rows there were detected benefits to the crop and these effects increased when the chemical control was used for weed management.

Cytogenetic analysis of seven species of Eucalyptus L'Hér. (Myrtaceae)

Seven species of the genus Eucalyptus were studied cytogenetically (E. deanei, E. dunni, E. grandis, E. maculata E. propinqua, E. saligna and E. tereticornis). The species showed a symmetrical karyotype with 2n=22 chromosomes, with chromosome length ranging from 0.58 μm to 1.39 μm. Karyotypic analysis indicated homogeneity of morphology and of chromosome number for most of the species of this genus studied here, although casual disploid species with 2n=24 have been found in previous studies. According to these data, a basic number of x=11 was established for this genus. The evolutionary tendency probably occurred by structural alterations (deletions, duplications, additions and translocations) and in some cases by aneuploid chromosome alterations.

Anatomical and biochemical characterization of the calcium effect on eucalyptus urophylla callus morphogenesis in vitro

Calcium chloride concentrations from 0.0 to 12.12 mM were added to the culture medium and calcium content in calluses were determined directly by X-ray fluorescence spectrometry, a non-destructive method, allowing the processing of the same tissue for histological analysis. A multivariate statistical analysis (PCA - Principal Components Analysis) grouped the treatments into 5 blocks and indicated the most responsive group. Lack of calcium supply caused a complete absence of a morphogenic process and tissue collapse. An increase in calcium concentration gave higher total protein and sugar contents, an increase in peroxidase specific activity and changes in the histological characteristics. It was possible to verify that calcium stimulated globular somatic embryo formation at concentration of 6.62 mM.

Efeitos da nutrição mineral na competição inter e intraespecífica de Eucalyptus grandis e brachiaria decumbens: 1- crescimento

The present work was conducted with the objective to study the effects of mineral nutrition on Eucalyptus grandis and Brachiaria decumbens (#BRADC) growth, when submitted to inter- and intra-specific competition. The treatments consisted of two plants of Eucalypts/pot, two plants of BRADC/pot, and one plant of each species/pot. The plants were nourished with the Hoagland e Arnon (1950) complete solution; with no K, P or N; or only with either N, P or K. Sixty days after growing side by side, no effect of the inter- and intra-specific competition on eucalypts plant high, branch number and leaf dry mass was observed. When mineral nutrition did not limit plant growth (complete solution or solution with no K), intra-specific competition reduced on average, 23% of eucalypts root length, leaf area, and stem and root dry mass, and inter-specific competition reduced, on average, 75% of BRADC dry mass. When mineral nutrition became a limiting factor, no plant competition effect on the parameters studied was detected.

Paternity study of an interspecific natural hybrid of the genus Eucalyptus L'Hér (Myrtaceae) based on cytogenetic data

Three species of the genus Eucalyptus (E. dunni, E. grandis, E. saligna) and interspecific hybrid were studied cytogenetically. The Eucalyptus species and the hybrid showed a symmetrical karyotype with 2n=22 chromosomes, with chromosome length ranging from 0.67 to 1.39 μm. Karyotypic analysis indicated a homogenous morphology and chromosome number for the species and the hybrid studied here. Based on the karyotype asymmetry data, together with the chromosome morphology results, the hybrid presented close similarity to E. saligna, suggesting that the latter is one of the parental species involved in the production of the hybrid.

Recuperação de povoamento de Eucalyptus urophylla com aplicações de nitrogênio, potássio e calcário dolomítico

A plantation of Eucalyptus urophylla, established in 1984 on a Quartzpsamment Soil, at Altinopolis, São Paulo State, presented in 1989 an medium increment of the cylindrical volume of the trees of 13.97 m 3/ha/year and also showed deficiency symptoms of potassium, calcium and magnesium. At 4.33 years after planting were applied two doses of nitrogen (0 and 10 g/tree of N), potassium (0 and 20 g/tree of K 2O) and of dolomitic lime (0 and 500 g/tree), combined in a randomized block design arranged in a factorial way (2 × 2 × 2), with four replications, in order to study the recuperation of the tree growth rate. Evaluations of the trees growth were performed at 4.33, 5.67, 6.75 and at 7.58 years when cutting the plantation was done. The soil fertility and leaf concentrations of N, K, Ca and Mg were analyzed before and after the application of the treatments. The accumulation and distribution of biomass and of these nutrients in the tree components and in the litter, and the economic studies referring to the volumes of wood were also evaluated 7.58 years after planting. Potassium fertilization increased the mass of potassium in stemwood, stembark, foliage, fruits and litter, but the increase was greater in foliage and in the stembark. Liming increased the mass of magnesium in stemwood, stembark, foliage and litter, but the increase was greater in the litter. There was no isolated effect of nitrogen fertilization. The growth rate of trees was recovered with the potassium application, which increased the mean annual increment (MAI) of cylindrical volume from 13.85 m 3/ha/year without fertilizer to 16.82 m 3/ha/year. At 7.58 years after planting, there was observed an effect of the N x K x dolomitic lime interaction in the wood production. In the absence of nitrogen and dolomitic lime, the application of potassium increased the real volume of 43% and there was a gain of US$ 86.79/ha.

Envelhecimento acelerado em sementes de Eucalyptus grandis Hill ex Maiden classificadas por tamanho

Two experiments of seed ageing of Eucalyptus grandis Hill ex Maiden were carried out at laboratory conditions. In the first experiment, two seed lots were classified in two square mesh screens (< 0.84 to ≥ 0.71 mm and < 0.71 mm to ≥ 0.59 mm), submitted to 42 °C and 100% relative humidity, for 0, 8, 12, 24, and 32 hours of stress treatments. Seed germination tests were done with just aged seeds. The results showed that the stress period was not sufficient for ageing the seeds. In the second experiment, the best quality seed lot was separated in the same two sizes. The seeds were submitted under the same stress conditions of the first experiment but for 0, 48, 72, and 96 hours. The germination test was done right after the seeds were taken from the stress conditions, and with stored seeds at dry chamber (2 weeks and 26 months). It was concluded that both seed sizes reached 30% of moisture content under 72 hours of ageing conditions and showed significant germination losses when compared with seeds without ageing (control), as the moist seeds as the stored ones at dry chamber, for both 2 weeks and 26 months.

Efeito da densidade de plantas de Bracharia decumbens Stapf sobre o crescimento inicial de mudas de Eucalyptus grandis W. Hill ex Maiden

In order to verify the effect of Brachiaria decumbens plant density on the initial growth of Eucalyptus grandis plants, one assay was conducted under semi-controlled conditions of soil fertility and humidity. Dark red Latossol, collected from the arable layer, was used as substrate in 50 liters amianthus cement boxes. One seedling of Eucalyptus was planted in each box. Fifteen days later, seedlings of B. decumbens were transplanted on the same box. The treatments consisted of 0, 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 60, 80, 100, and 120 plants of B. decumbens. The experimental design was the complete randomized design, with 15 treatments and four replicates. The Eucalyptus plants that lived with B. decumbens were evaluated for stem diameter, plant high, leaf area, and dry weight of stem, branches and leaves. B. decumbens plants were evaluated for dry weight of aerial parts. B. decumbens, at the density level of four plants per m 2 and over, reduced of the initial growth of the Eucalyptus plants. B. decumbens reduced on average 27.78% stem diameter, 18.47% plant high, 70.56% leaf number, 63.26% leaf area, and 55.22%, 77.29% and 55.30% of stem, branch and leaf dry weight, respectively. Plant high was not a good parameter to evaluate the B. decumbens interference.

Avaliação da variabilidade genética em quatro gerações de Eucalyptus urophylla S.T. Blake por meio do marcador molecular RAPD

Molecular markers have gradually replaced morphological markers in population studies. The advantages of molecular markers are the speed and precision of evaluations, mainly for long cycle cultures, where determinate traits can take years to manifest. The principle objectives of this research were to assess variability and genetic distances in four generations of Eucalyptus urophylla and provide data that help with the continued improvement of these materials. The populations can be found at the Experimental Forestry Sciences Station, Anhembi, SP, belonging to the College of Agriculture Luiz de Queiroz of São Paulo University. The initial base population was introduced by seeds collected in indonesia and designated P0 generation. The subsequent segregated generations, derivatives of recombination starting with open pollination, were designated P1, P2, and P3. One hundred and seventy four individual trees representing the four generations were analysed. The RAPD technique allowed the identification of 86 loci that were analysed with the Jaccard Coefficient, generating a genetic similarity matrix, permitting the estimation of genetic distances. The genetic distance of generation PO was 0.3338333, P1 was 0.336824, P2 was 0.40000, and P3 was 0.381093. In percentage terms the genetic distances between individuals grew in relation to base population, being 0.15% for generation P1, 18.93% for P2, and 13.31% for P3. This shows an increase in genetic variability with the advance of the program, despite the selective processes. From this came the belief that the initial base population was resulting from seed collection from isolated trees. These populations, although going through successive selections, had a high cross efficiency through satisfactory pollination, which then permitted genetic variation to increase, the outcome of effective recombination between individuals. Generations P2 and P3 gave a better perspective for the continuance of the improvement program due to the high number of different groups with standard genetic distances of 35%. The selections made between the diverse genetic groups allowed the efficient use of genetic variability evaluation.

Caracterização silvicultural, botânica e avaliação da variabilidade genética por meio do marcador molecular RAPD em um teste de progênies de Eucalyptus urophylla S. T. Blake

Molecular markers have recently been incorporated into genetic improvement programs. They are already considered as powerful tools with several different uses, for instance the monitoring of genetic variability in tree populations. The main objectives of this study were to evaluate genetic variability in Eucalyptus urophylla progenies and together with silvicultural and botanical information, provide assistance to the improvement program. The Eucalypts population is located at the Experimental Forestry Sciences Station, Anhembi, SP, which belongs to the College of Agriculture Luiz de Queiroz. Sixty-nine progenies were analysed representing one individual by family in open pollinated Eucalyptus urophylla trees. The RAPD technique allowed the identification of 72 loci that were analysed using Jaccard's Coefficient generating a genetic similarity matrix to permit estimation of genetic distances. The results obtained showed genetic distance between individuals of 0.40 with 12 groups of genetic variability using a standardised distance of 40%. The progenies showed different bark patterns, allowing the establishment of bark groups. The groups formed based on genetic distances obtained using DNA analysis did not correspond to those based on bark pattern. Genetic selection was simulated in which silvicultural and genetic variability data were linked, thus avoiding excessive variability losses. The simulation of controlled crossings allowed the maximum genetic difference to be obtained linked with height and individual bark roughness.