Do Atopobium vaginae, Megasphaera sp. and Leptotrichia sp. change the local innate immune response and sialidase activity in bacterial vaginosis?

Objectives: To investigate if the participation of Atopobium vaginae, Megasphaera sp. and Leptotrichia sp. in the bacterial community of bacterial vaginosis (BV) is associated with distinct patterns of this condition. Methods: In this cross-sectional controlled study, 205 women with BV and 205 women with normal flora were included. Vaginal rinsing samples were obtained for measuring the levels of pro-inflammatory cytokines and bacterial sialidases. Real-time PCR was used to quantify the BV-associated bacteria and to estimate the total bacterial load using the 16S rRNA. Principal component analysis (PCA) using the measured parameters was performed to compare the BV samples with lower and higher loads of the species of interest. Results: Higher bacterial load (p<0.001), levels of interleukin 1-β (p<0.001) and sialidase activity (p<0.001) were associated with BV. Women with BV and higher relative loads of A vaginae, Megasphaera sp. and Leptotrichia sp. presented increased sialidase activity, but unchanged cytokine levels. PCA analysis did not indicate a different pattern of BV according to the loads of A vaginae, Megasphaera sp. and Leptotrichia sp. Conclusions: Greater participation of A vaginae, Megasphaera sp. and Leptotrichia sp. in vaginal bacterial community did not indicate a less severe form of BV; moreover, it was associated with increased sialidase activity.

Sialidase activity in aerobic vaginitis is equal to levels during bacterial vaginosis

Objective: To evaluate levels of proinflammatory cytokines and sialidase activity in aerobic vaginitis (AV) in relation to normal vaginal flora and bacterial vaginosis (BV). Study design: In this cross-sectional study, a total of 682 consecutive non-pregnant women attending the gynecology service were assessed and 408 women were included. Vaginal rinsing samples were collected from 223 women with microscopic finding of BV (n = 98), aerobic vaginitis (n = 25) and normal flora (n = 100). Samples were tested for interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, and sialidase activity. Results: Compared to women with normal flora, vaginal levels of IL-1β were highly increased in both BV and AV (p < 0.0001). Significantly higher vaginal IL-6 was detected in AV (p < 0.0001) but not in BV, in relation to normal flora. Women with AV also presented increased IL-8 levels (p < 0.001), while those with BV presented levels similar to normal flora. Sialidase was increased in BV and AV compared with the normal group (p < 0.0001) but no difference in sialidase activity was observed between BV and AV. Conclusion: A more intense inflammatory host response occurs for AV than for BV when compared with normal flora. Furthermore, the increased sialidase activity in AV and BV indicates that both abnormal vaginal flora types can be harmful to the maintenance of a healthy vaginal environment. © 2012 Elsevier B.V.

Entre o sofrimento e a esperança: A reabilitação da incontinência urinária como componente interveniente

This is a qualitative study seeking to understand Brazilian women's experience of urinary incontinence (UI) and design a representative theoretical model for the experience. Theoretical saturation occurred after analysis of the 18th non-directive interview in accordance with Grounded Theory. Two phenomena emerged: living with the challenges of UI and experiencing the hope and disappointment of rehabilitation from UI. Upon re-alignment of the components, the core category emerged, namely: between suffering and hope - rehabilitation from urinary incontinence as an intervening component. From the analysis in light of symbolic interactionism, pregnancy and vaginal birth were observed to be symbols of women's vulnerability to the suffering from living with the moral and physio-psychosocial challenges of UI. It is also inferred that the lack of consideration of the Unified Health System (SUS) in investing in the process of rehabilitation from UI may be having a negative effect on the incentive programs for promoting vaginal birth. Most of all, it reveals the ongoing suffering of women with UI, most of whom do not have access to rehabilitation due to the lack of programs geared to the real needs of these users of the Unified Health System.

Vitamina E em dietas para reprodutoras de tilápia-do-nilo (Oreochromis niloticus)

Pós-graduação em Aquicultura - FCAV

Extração semi-automática de rodovias no espaço-objeto: uso integrado de um estéreo par de imagens aéreas e um MDT

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Vale de ferro: estudo histórico e estético das estações ferroviárias do Vale do Paraíba Paulista

Pós-graduação em Artes - IA

Projeto e implementação com chaveamento de reguladores fuzzy Takagi-Sugeno para um conjunto de pontos de operação

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Um modelo de unit commitment hidrotérmico para o ambiente de mercados de energia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Projeto de reguladores fuzzy Takagi-Sugeno utilizando as condições iniciais da planta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Modos deslizantes discretos em sistemas incertos com atraso na computação do sinal de controle

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Controle com modos deslizantes aplicado em sistemas com atraso e acesso somente à saída

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A interferência da tarefa dupla, motora e cognitiva, no andar de pacientes com doença de Parkinson

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Descrição e comparação de dois tipos de chute no futebol feminino através de variáveis angulares

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise cinemática de cobranças de pênaltis

Pós-graduação em Ciências da Motricidade - IBRC

Utilização de diferentes culturas lácteas na fabricação da mussarela de leite de búfula

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)