Correlation between volume of apical periodontitis determined by cone-beam computed tomography analysis and endotoxin levels found in primary root canal infection

This clinical study was conducted to correlate the levels of endotoxins and bacterial counts found in primary endodontic infection with the volume of periapical bone destruction determined by cone-beam computed tomography (CBCT) analysis. Moreover, the levels of bacteria and endotoxins were correlated with the development of clinical features. Twenty-four root canals with primary endodontic disease and apical periodontitis were selected. Clinical features such as pain on palpation, pain on percussion, and previous episode of pain were recorded. The volume (cubic millimeters) of periapical bone destruction was determined by CBCT analysis. Endotoxins and bacterial samplings were collected by using sterile/apyrogenic paper points. Endotoxins were quantified by using limulus amebocyte lysate assay (KQCL test), and bacterial count (colony-forming units [CFU]/mL) was determined by using anaerobic culture techniques. Data were analyzed by Pearson correlation and multiple logistic regression (P < .05). Endotoxins and bacteria were detected in 100% of the root canal samples (24 of 24), with median values of 10.92 endotoxin units (EU)/mL (1.75-128 EU/mL) and 7.5 × 10(5) CFU/mL (3.20 × 10(5)-8.16 × 10(6) CFU/mL), respectively. The median volume of bone destruction determined by CBCT analysis was 100 mm(3) (10-450 mm(3)). The multiple regression analysis revealed a positive correlation between higher levels of endotoxins present in root canal infection and larger volume of bone destruction (P < .05). Moreover, higher levels of endotoxins were also correlated with the presence of previous pain (P < .05). Our findings revealed that the levels of endotoxins found in root canal infection are related to the volume of periapical bone destruction determined by CBCT analysis. Moreover, the levels of endotoxin are related to the presence of previous pain.

Avaliação da capacidade seladora apical de um adesivo dentinário, acrescido ou não de hidróxido de cálcio, comparada à de um cimento endodôntico à base de hidróxido de cálcio, na obturação de canais radiculares

Pós-graduação em Odontologia Restauradora - ICT

Efeito dos lasers de Nd:YAG e de Er:YAG na adaptação das obturações de canais radiculares: análise do selamento marginal apical

Pós-graduação em Odontologia Restauradora - ICT

Influence of irrigation and intracanal dressing on the healing process of dogs' teeth with apical periodontitis.

Dogs' teeth with apical periodontitis were treated endodontically, Dakin's solution being used in an experimental group as the irrigation solution, and camphorated paramonochlorophenol as an intracanal dressing. For a second group of teeth, the irrigation solution used was physiologic saline, dry cotton only being placed into the pulpal chamber to take the place of an intracanal dressing. In a second visit, overinstrumentation and a new application of the same kind of dressing were performed, the root canals being then filled with gutta-percha cones and zinc oxide-eugenol cement. Other specimens were treated, in one visit, where physiologic saline or Dakin's solution were the irrigation solutions. The animals were sacrificed 6 months after the obturation of the root canals. Histologic exams showed better results for the experimental group where Dakin's solution and camphorated paramonochlorophenol had been used, with signs of repair characterized by newly formed cementum and bone tissue, as well as a marked reduction in size of the periapical lesions. No differences were seen in the results with physiologic saline or Dakin's solution as irrigants.

Root reconstructed with mineral trioxide aggregate and guided tissue regeneration in apical surgery: a 5-year follow-up

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Poda apical para produção de frutos e sementes de abóbora

The tip pruning stimulates the emission of lateral shoots, thus can produce higher number of flowers, fruits and seeds. This work aimed to evaluate the effect of apex pruning on fruit and seed production in pumpkin. The treatments consisted of plants without pruning, with pruning in the sixth, eighth and tenth node of main stem. The experimental design was a randomized block, with six replications. It was studied a line of pumpkin of the Germplasm Bank of the Universidade Estadual Paulista/Faculdade de Ciências Agronômicas. The characteristics evaluated were: number of branches per plant and fruit position in branches of the plant production (number and weight) of fruits per plant, fruit yield, fruit average weight, fruit length and diameter, seed weight per fruit, seed yield and quality (germination test, first count, thousand seed weight, emergence, speed of emergence and accelerated aging). There was a significant difference only for number of secondary branches per plant and thousand seed weight, with larger values for plants that have not been pruned. It was obtained high average of germination (94%) and good fruit (16.9 t ha-1) and seed (148 kg ha-1) yield. The apex pruning does not influence the production of fruits and seeds, as well as the physiological seed quality in pumpkin.