Análise de atividade epileptiforme com uso simultâneo de eletrencefalografia e ressonância magnética funcional

The use of multimodal neuroimaging techniques has been helpful in the investigation of epileptogenic zone in patients with refractory epilepsies. This work aims to describe an ictal event during EEG-fMRI performed simultaneously in a 39-year-old man with refractory epilepsy. The EEG data were recorded at a sampling rate of 5 kHz, using a BrainAmp (BrainProducts, München, Germany) amplifier, with 64 MR (magnetic resonance) compatible Ag/AgCl electrodes. MR images were acquired using a 3T scanner in 3 sequences of 6 minutes of echo-planar images (EPIs), with TR = 2s, being the last sequence stopped after the ictal event. The EEG was corrected for gradient and pulse artifacts using the Brain Vision Analyzer2 software (BrainProducts), and the functional images were realigned, slice-timing corrected, normalized and smoothed. The start of the ictal changes was used for the evaluation of the BOLD response in MR images, using a t-test with a minimum cluster of 5 voxels, p <0.005 (T>2.5). The patient had a partial complex seizure, as noted by neurologist. The fMRI data showed positive BOLD responses (activation) in dysplastic areas, but showed the most significant activation outside the lesion, in areas compatible with secondary spread of the epileptic focus, probably caused by motor reaction also observed during the seizure. As a conclusion, we note that the technique of EEG-fMRI can detect the epileptogenic zone in patients with refractory epilepsy, but areas of dissemination of primary epileptogenic focus may show significant activation, introducing additional difficulties to the interpretation of the results

O ensino de física nos primeiros anos da educação básica: possibilidades lúdicas e experimentais

We discuss in the work the importance of experiential activities for Physics Teaching in the first years at Elementary Education. For this we start from the experience provided by the Teaching Project Initiation under Pibid CAPES, linked to the Department of Education from the Institute of Biosciences and activities of the Graduate Diploma in Physics Supervised, at Rio Claro Campus, São Paulo State, in partnership with Basic Education schools of the city. Based on some patterns and studies on the Teaching of Science in Elementary Education and particularly in the Ferreira’s work with Instrumentation for Physics Teaching, Electrostatic was chosen as base theme. We opted for the use of didactic experiments that provided in its construction the utilization of low cost materials, easy access and portability. Teaching strategies were aimed at inserting experiential activities during Elementary School I (first and third years of elementary school), intending to rescue the trial of Physics Education and Science for Basic Education. These experimental teaching activities were analyzed in this paper from the perspective of playfulness. We argue that experimentation with recreational attributes when properly used provides the interaction of individuals with the knowledge of Physical Sciences

Ação antimicrobiana da clorexidina gel e líquida e do hipoclorito de sódio

The purpose of this study was to evaluate in vitro antimicrobial activity of chlorhexidine gel and liquid 2%, and 2% sodium hypochlorite on Candida albicans, Enterococcus faecalis, Escherichia coli in root canals. For this, we used 40 human single-rooted teeth were divided into 4 groups (n = 10) according to assist the chemical used: 1) 2% chlorhexidine liquid, 2) 2% chlorhexidine gel, 3) sodium hypochlorite 2%, and 4) physiological saline (control). Content were collected immediately after root canal instrumentation and after 7 days of biomechanical. For the samples was conducted to evaluate the antimicrobial activity and the results were subjected to statistical analysis of Kruskal-Wallis and Dunn's test with a significance of 5%. It was found that chlorhexidine gel and liquid as well as sodium hypochlorite were effective against the microorganisms tested

Atividade espontânea e estimulação do inseto (T. molitor)

The heart of the insect is essentially myogenic, in others words, electrical activity and spontaneous contractions are generated by their muscle cells. However, the nature of pacemaker activity is not yet fully elucidated. One aspect that deserves attention, beyond the pacemaker activity, is the amplitude of heart contractions of the insect. For this, it is necessary that the heart to be stimulated electrically to the frequency to be controlled. In the present work, it was developed a method of stimulation (preparation and instrumentation). The contractile activity of the heart of the insect remained regular when stimulated at frequencies lightly above the spontaneous frequency. To characterize excitability of the preparation the intensity-duration curve was determined

Tomografia por Biosusceptometria de Corrente Alternada (tBAC): desenvolvimento, caracterização, implementação

The Biosusceptometry AC (BAC) is a research tool that has been extensively explored by the group Biomagnetism IBB-UNESP for monitoring of the gastrointestinal tract, its response to a known drug or in vivo performance of solid dosage forms. During this period the BAC, which has the characteristics of high sensitivity and low cost, has been developed primarily for recording signals contraction of activity and traffic human gastrointestinal tract. With the possibility of producing images with this instrumentation, it was possible to evaluate different situations in vitro and in vivo for physiological studies and pharmaceuticals. Considering the good performance of this system to produce planar images, the first aim of the BAC system tomography (TBAC) was to evaluate the system performance of BAC to produce tomographic images of phantoms ferromagnetic for a single channel system. All these applications were only possible because of their sensitivity to materials of high magnetic suscepitibility as ferrite, which allow to produce an electrical signal proportional to the variation of the magnetic flux generated by the presence of magnetic marker next to a first-order gradiometer. Measuring this variation at various points was possible to generate planar images that recently came to be produced in systems with multiple detectors, said multi-channels. From planar images, also producing tomographic images of simulators BAC bars in a system of 13 channels using only the center channel, with good results when applied to simple objects as one and two bars. When testing the resolution of the system with more elaborate forms the quality and resolution of images reconstructed is not satisfactory, which would be solved by increasing the spatial sampling rate and hence the acquisition time. The present system works with an acquisition time of about five hours. Whereas this system will be applied for in vivo experiments, the acquisition time became a ...

Análise do tempo de ação de medicações intracanais sobre candida albicans, enterococcus faecalis, escherichia cole em canais radiculares

The purpose of this study was to evaluate in vitro the antimicrobial activity of intracanal medications against Candida albicans, Enterococcus faecalis, Escherichia coli present in root canals. It was used 24 single root human teeths, contaminated for 28 days and prepared with physiological saline solution as irrigation solution. The teeth were divided into 2 groups according to the intracanal medication used: 1) 2% gel chlorhexidine, 2) sterile and pyrogen free physiological saline solution. Samples were taken of the root canals immediately after instrumentation, 7 days after intracanal medication and 7 days after removal of intracanal medication. For all samples the antimicrobial activity was performed by plating method. All results were submitted to Mann-Whitney and Dunn's test with significance of 5%. There was significant reduction of microorganisms after instrumentation and the intracanal medication of 2% gel chlorhexidine completely eliminated C. albicans and E. coli, and significantly reduced E. faecalis. It was concluded that 2% gel chlorhexidine as intracanal medication for 7 days was effective on microorganisms

Análise dos procedimentos metodológicos de dimensionamento e execução de parede diafragma

The present paper conducts an analysis of all steps for the construction of diaphragm walls, from obtaining the soil parameters until its execution. Construction stages of diaphragm wall are detailed. The paper presents a detailed analysis of the main available calculation methods to obtain the soil parameters and the design of the diaphragm wall. A case history extensively documented in previous research and present in international symposiums was chosen for an analysis of construction process and excavation’s instrumentations, as well as emphasizing the importance of temporary works and construction sequence for deep excavation with cast in-situ diaphragm walls. In general, it is possible to conclude the main difficulty and uncertainty of the dimensioning are obtaining the soil parameter for diaphragm wall design. And, it is also possible to conclude the monitoring and execution control are paramount to the success of the diaphragm wall construction

Caracterização in vitro de técnica biomagnética empregando magnetômetros 3D para avaliar tempo de trânsito esofagiano

The biomagnetic techniques use different magnetic field detectors to measure parameters of the human physiology. Those techniques present the advantage of being noninvasive and radiation free. Among them we can show up the Superconducting Quantum Interference Device (SQUID), the Current Alternate Biosusceptometry (ACB) and, more recently, the employment of anisotropic magnetoresistive sensors. Those magnetic sensors have a low cost and good sensitivity to measure different physiological parameters using magnetic markers. The biomagnetic techniques have being used successfully through study on the characteristics of the gastrointestinal tract. Recent research, the magnetoresistors were used to evaluate the transit time and localization of magnetic sources in different parts of the gastrointestinal tract. The objective of this work is the characterization, with in vitro tests, of a biomagnetic instrumentation using two 3-axis magnetoresistors arranged in a gradiometric coplanar setup to evaluate esophageal transit time, analyze and compare the results of experimental signals and the magnetic theory, as well as evaluate the instrumentation gain with use of tri-axial sensor front to the mono-axial sensor. The instrumentation is composed by two three-axis sensing magnetometers, precision power supply and amplifier electronic circuits. The sensors fixed in a coplanar setup were separate by distance of 18 cm. The sensitivity tests had been carried through using a cylindrical magnet (ø = 4 mm and h = 4 mm) of neodymium-iron-boron (grid 35). The tests were done moving the permanent magnet on the sensors parallel axis, simulating the food transit in... (Complete abstract click electronic access below)

O uso de experimentos didáticos como ferramenta para o ensino de física nos anos iniciais da educação básica

When it comes to teaching physics in the early years of elementary school, the first question is: but there are opportunities to teach concepts to children of such complexity? This study sought to examine approaches and strategies to enter the Basic Education in Physics. To this end, we used low cost materials testing, taking as its starting point the work of Ferreira (1978) instrumentation for Teaching Physics, particularly with the theme electrostatics. The present study was made from the use of prototypes developed with the materials cited. Observations were made in the classroom looking for, from the records of teaching, analyzing the behavior of children and their arguments, possibilities for Physics Teaching this age group as well as some evidence of their cognitive development. In teaching discussions were held with students of the early years of elementary school involving conceptual and phenomenological aspects, adapting such knowledge at the level of logical and mathematical thinking that was still under development. The work shows that it is possible to work on electrostatic physical concepts with children belonging to the age group of nine to ten years. With the support of the group Pibid Physics city of Rio Claro, I realize my observations and practices at the Municipal School Marcelo Schmidt, which proved to be available and open for acceptance of this proposal

Avaliação in vitro da ação de substâncias químicas auxiliares, clorexidina e medicações intracanais sobre C. albicans, E. faecalis, E. coli e sua endotoxina em canais radiculares: [recurso eletrônico]

The purpose of this study was to evaluate the antimicrobial activity of chlorhexidine gel 2% as auxiliary chemical substance on the biomechanical preparation (BMP) and medication intracanal (ICM) on C. albicans, E. faecalis, E. coli and their endotoxin in root canals. We used 48 single-rooted human teeth divided into four groups according to dressing ICM: 1) Ca(OH)2 + pyrogen-free saline solution; 2) 2% chlorhexidine gel (CLX); 3) Ca(OH)2 + CLX, and; 4) pyrogen-free saline solution (control group). Were collected the contents of root canals to confirm the presence of microorganisms (confirmation), immediately after instrumentation (1st collection), after 7 days of the BMP (2nd collection), after 14 days of the action of ICM (3rd Collection) and 7 days after removal of the ICM (4 th collection). Were performed: the evaluation of antimicrobial activity and the content analysis of endotoxins for all sampling tests. The results were statistically analyzed using Kruskall-Wallis and Dunn tests with a significance of 5%. It was found that the CLX as auxiliary chemical substance has significantly reduced microorganisms confirmation collection when compared. In relation to the neutralization of endotoxin, it was found that the 1st and 2nd collections presented a decrease of 92.03% and 98.10% in mean percentage respectively, when compared to the confirmation collection. In the 3rd and 4th samplings, the Ca (OH)2 + CLX group showed the best results. It was concluded that the BMP and the ICM were able to eliminate the tested microrganisms, however, they were not able to completely eliminate endotoxins root canal

O experimento e o lúdico: reflexões sobre atividades didáticas experimentais para o ensino de Física e a ludicidade

In this work, is discussed the importance of experimental activities in Education of Physics in Basic Education from the experience of Supervised Stage and the work with the Initiation of Teaching in the Program PIBID CAPES, in this case linked to the Department of Education of the Institute of Biosciences, Campus Rio Claro, São Paulo State, in partnership with three schools of basic education in the city. At first, based on the work of Ferreira with Instrumentation for Teaching Physics, consider the electrostatic theme. The focus is the searching for equipments constructed with low cost materials and easy access. Were developed and promoted strategies of the use of such materials in Physics’ Teaching, with a view to reintroducing experimental teaching activities in Public Basic Education, in the form Workshop Learning and Teaching of Physics, as well as lessons of Laboratory with the help of script and also in the form of an Experiments Library. These experimental activities were critically analyzed with a focus on playfulness. The focus of work in experimental activities for the Physics’ Teaching allowed to redeem, critically, didactic materials. From the standpoint of playfulness, playful moments and interaction of individuals with the knowledge that provides the experimental material when used strategically, they seem to be of great relevance to the Physics’ Teaching in Basic Education. It is possible to use experiments in Physics’ Teaching in public schools

Avaliação do efeito da gastrectomia na motilidade e no esvaziamento gástrico em ratos por biosusceptometria de corrente alternada

The study of gastrointestinal tract (GIT) functions is necessary due to the increasing number of pathologies associated with it. Directly influencing the quality of life, the gastrointestinal tract provides a number of parameters that, when analyzed, allow us to describe its dysfunctions. Thus, many techniques can be combined to obtain these properties related to the GIT. However, these techniques are often invasive, require surgery, catheter insertion, or to build a temporal model of these functions, require the sacrifice of animals in a series of data collection. The technique used in this study has the advantage of having a low operating cost, being free of ionizing radiation, non-invasive and is known as biosusceptometry AC (BAC), used to evaluate the properties of the GI tract by monitoring the position and concentration of materials magnetically marked. The sensor consists of two pairs of coils, one reference and one for detection. A fixed base line separates the sensing and reference coils, and also functions as support for the instrumentation. It is also important to note that the detection coils are arranged in a first order (subtraction) gradiometric way. The objective of this study was to analyze the effects of gastrectomy in gastric emptying and gastrointestinal transit time of solid food in rats using a BAC system associated with magnetic markers. To realize this study was constructed a dedicated BAC sensor, built to analyze these GIT properties. Data acquisition was obtained by aligning the magnetic sensor with the stomach and colon of the animal at pre-determined intervals. Thus, when approaching the magnetic material of the sensor, the balance created between the two sides of the sensor is broken. This imbalance can be measured, digitized and acquired. Tracer was used as a ration magnetically marked with ferrite... (Complete abstract click electronic access below)

Monitoramento do perfil microbiológico de dentes com infecção endodôntica primária durante terapia com diferentes medicações intracanal

Pós-graduação em Odontologia Restauradora - ICT

Desenvolvimento de sistema eletrônico para instrumentar um DPL em ensaio dinâmico de resistência do solo

Pós-graduação em Engenharia Elétrica - FEIS

Morphology and adhesion on blood components in root surfaces treated by a piezoelectric ultrasonic: an in vitro study

The aim of this study was to evaluate the morphology and adhesion of blood components on root surfaces instrumented with piezoelectric ultrasonic Piezon Master Surgery. Methods 10 teeth were used in this study. The teeth had their proximal divided into four areas that received different treatments: Group 1: untreated control Group 2: scaling with manual instrument; Group 3: scaling with ultrasound; Group 4: Scaling with manual instruments and ultrasound. We obtained 20 samples, 10 of which were used to analyze the morphology and the other 10 were used for analysis of adhesion of blood components. The specimens were analyzed by scanning electron microscopy. Photomicrographs were analyzed by the scores of adhesion of blood components and the index of root morphology. The results were statistically by the Kruskall-Wallis and Mann-Whitney with a significance level of 95%. Results The morphological analysis showed that the Group 1 had a surface unchanged in relation to other groups (Group 1 X Group 2 = 0.0025; Group 1 X Group 3 = 0.0003; Group 1 X Group 4 = 0.0003) and Group 2 presented a smoother surface compared to Group 1 and groups instrumented with ultrasound (Group 2 X Group 3 = 0.0025; Group 2 X Group 4 = 0.0025) there were no statistical differences between the Groups 3 and 4. analysis of adhesion of blood components showed that the Groups 2, 3 and 4 had no statistically significant differences between themselves, but more biocompatible surfaces promoted the surface untreated control (Group 1 X Group 2 = 0.02; Group 1 X Group 3 = 0.04; Group 1 X Group 4 = 0.005). Conclusion The instrumentation with piezoelectric ultrasonic promoted an irregular root surface, but did not negatively affect the adhesion of blood components.