Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples

Background. The use of methods, both sensitive and specific, for rabies diagnosis are important tools for the control and prophylaxis of the disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed materials. Additionally, molecular techniques have been used for epidemiological studies and to gain a better knowledge of viral epidemiology. Findings. The aim of this work was to evaluate the RT-PCR and hnRT-PCR for rabies virus detection in original tissues stored at -20°C for different periods considering their use for rabies virus detection in stored and decomposed samples. RT-PCR and hnRT-PCR were evaluated in 151 brain samples from different animal species, thawed and left at room temperature for 72 hours for decomposition. The RT-PCR and hnRT-PCR results were compared with previous results from Direct Fluorescent Antibody Test and Mouse Inoculation Test. From the 50 positive fresh samples, 26 (52%) were positive for RT-PCR and 45 (90%) for hnRT-PCR. From the 48 positive decomposed samples, 17 (34, 3%) were positive for RT-PCR and 36 (75%) for hnRT-PCR. No false-positives results were found in the negatives samples evaluated to the molecular techniques. Conclusion. These results show that the hnRT-PCR was more sensitive than RT-PCR, and both techniques presented lower sensibility in decomposed samples. The hnRT-PCR demonstrated efficacy in rabies virus detection in stored and decomposed materials suggesting it's application for rabies virus retrospective epidemiological studies. © 2008 Arajo et al; licensee BioMed Central Ltd.

Estudio de la compactación de un Ferralsol manejado con un sistema de no laboreo en Selvíria (MS, Brasil)

No tillage management is widely used by the Brazilian farmers and technicians like a soil conservation system, which reduces the soil losses by water erosion, increasing the infiltrated and stored water in soil, warranting environmental sustainability. No-tillage system does not invert the soil; it causes the creation of a compacted layer. The samples were taken in the agricultural year 2005/2006 in an Oxisoil at Selviria (MS/Brazil). The tillage management in the last 15 years was no-tillage system with crop rotation (maize -Zea mays L./bean - Phaseolus vulgaris L.). The analyzed soil physical properties were bulk density (BS), gravimetric water content (U) and mechanical resistance to penetration (RP) at three depths: 0-0.10 m, 0.10-0.20 m and 0.20-0.30 m. The samples were taken in a mesh with 117 sampled points covering an area of 0.16 ha. It was investigated the existence of compacted soil layer, using the mechanical resistance to penetration to 0.60 m depth with soil water content at field capacity. The data shows low coefficient of variation, except the resistance penetration data. Bulk density and gravimetric water content has a normal distribution. Only resistance to penetration at 0.10-0.20 m depth layer has a normal distribution. The correlation between different properties was low. The bulk density increases with depth; the increase of the values of soil bulk density are consistent with data in other papers, indicating there are not compaction problems for the crop development at the study area. Most of the values of resistance to penetration are lower than 2 MPa, being this value restrictive for root development. The analysis of resistance to penetration profile 0 to 0.60 m shows a compacted layer between 0.20-0.30 m. This compacted layer was caused by the conventional tillage system used at this area before the use of no-tillage system. The soil bulk density has higher values at the upper area, that it shows higher values of soil compaction. Although the values of bulk density and resistance to penetration are high, the area does not show great problems of soil compaction.

Dimensional alterations and solubility of new endodontic sealers

This study evaluated the dimensional alterations and the solubility of two experimental endodontic sealers based on Copaifera multijuga oil-resin (Biosealer) and castor oil bean cement (Poliquil), maintained in different storage solutions. Twenty specimens (3 mm diameter and 2 mm height) of each sealer were assigned to 2 groups (n=10) according to the storage solution: simulated tissue fluid (STF) or distilled water (DW). The specimens were stored in these solutions during 90 days, being removed every 30 days for weighting. The solutions were renewed every 15 days. The results were subjected to statistical analysis by Dunn's and Mann-Whitney tests (α=0.05). The solubility of Poliquil was higher in STF (38.4 ± 36.0) than in DW (28.4 ± 15.0), while Biosealer showed higher solubility in DW (34.61 ± 6.0) than in STF (18.59 ± 8.0). The storage solution influenced the behavior of sealers in relation to the weight variation (p=0.0001). Poliquil presented higher variation of weight independent of the solution (p=0.239). Biosealer also presented higher variation of weight regardless of the solution (p=0.0001). The solubility of Biosealer was different from that of Poliquil, but both sealers showed low solubility in STF. Under the tested conditions, neither of the materials were according to the ADA'S specification.

Postharvest decay development on guava stored under several controlled atmosphere conditions

The control of post-harvest fungal decay on guava (Psidium guajava L. 'Pedro Sato') stored under low oxygen controlled atmosphere (5 kPa) was compared with increasing concentrations of carbon dioxide in the atmospheres. The combination of high concentrations of carbon dioxide (1, 5, 10, 15 and 20 kPa) with low oxygen (5 kPa) did not result in additional decay control. The low oxygen level (5 kPa) was the main factor for controlling post-harvest fungal development which resulted in a very low percentage of fruits with symptoms of anthracnose and stylar end rot throughout cold storage, regardless of the CO2 concentration. After transfer to ambient conditions, only the atmospheres with 5 kPa O2 (control), 5 kPa O2 + 1 kPa CO2 and 5 kPa O2 + 5 kPa CO2 resulted in reduced incidence of stylar end rot (P<0.05). There was not a significant interaction among CA combinations and storage duration on the percentage and number of typical anthracnose lesions.

Protective effect of oregano (Origanum vulgar L.) and thyme (Thymus vulgaris L.) oleoresins in stored soybean oil

Purpose: The objective of this study was to evaluate the antioxidant effect of oregano and thyme extracts isolatedly and combinedly applied in soybean oil. Design/methodology/approach: Soybean oil containing 3,000 mg/kg of oregano and thyme oleoresins and the mixture of both, as well as soybean oil containing TBHQ (50 mg/kg) and soybean oil free of antioxidants, were subjected to accelerated oven test (60°C/10 days). Samples were collected every two days and analyzed as to their peroxide and conjugated diene values. Findings: The mixture of oleoresins and consequent increase of concentration were as effective as the antioxidant TBHQ. Practical implications: These studies may prove to be beneficial to the exploitation of natural antioxidant sources for the preservation and/or extension of raw and processed food shelf life. Therefore, they could also be applied in the area of pharmaceuticals for the protection of human life. Originality/value: This study offers information on the use of natural antioxidants as an alternative to the use of synthetic antioxidants, which might be considered toxic. © Emerald Group Publishing Limited.

Effect of reduced fungicide doses on control of soybean Asian rust and bean yield

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The fat body of termites: Functions and stored materials

The functions of the fat body in the different castes of termites, and accumulation of energy reserves, proteins and urates within this organ, are reviewed. The termite fat body is involved in multiple metabolic activities, including recycling of nitrogen. Termite fat body showed three different types of cells: adipocytes, urocytes and mycetocytes, the latter restricted to the species Mastotermes darwiniensis. Adipocytes synthesize and store lipids, glycogen and several proteins. These cells also elaborate important peptides, including some that act in immune processes. Urocytes are responsible for the storage of spherocrystals of urates, which vary quantitatively among the termite castes. The different metabolic functions of the fat body in the several castes and stages of termites are associated with specific adipocyte morphologies. The synthesis and storage of different compounds modify the structure of the fat body; this differentiation is coordinated by hormones involved with molting and reproductive cycles. © 2013 Elsevier Ltd.

Posição do racemo, do fruto e armazenamento na qualidade de sementes de mamona (Ricinus communis L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Complementação do seqüenciamento do genoma do Southern bean mosaic virus, isolado São Paulo, expressão da porção C-terminal da polimerase e produção de anti-soro policlonal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão em Escherichia coli e produção de antissoro policlonal para proteína P1 do Southern bean mosaic virus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Condutividade elétrica e composição mineral da solução de embebição de sementes de feijão armazenadas em duas temperaturas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização biofísica e produção de antissoro policlonal contra a capa proteica recombinante do Southem bean mosaic virus

Pós-graduação em Genética - IBILCE

Activity of some isoenzymatic systems in stored coffee grains

Considering the worldwide consumption of coffee, it is natural that throughout the history many people have dedicated the research to markers that contribute somehow on gauging its quality. This research aimed to evaluate the biochemical performance of arabica coffee during storage. Coffee in beans (natural) and in parchment (pulped) dried in concrete terrace and in dryer with heated air were packed in jute bags and stored in not controlled environmental conditions. Enzymatic activities of superoxide dismutase, catalase, peroxidase, polyphenoloxidase, esterase and lipoxygenase in coffee grains were evaluated at zero, three, six, nine and twelve months by means of electrophoresis. Independently of the drying method, the activity of isoenzymatic complexes highlighted deteriorative processes in stored grains of coffee. The treatments 60/40º C and 60º C used to reduce the water content imposed a greater stress condition, accelerated metabolism of natural coffee in the storage with decreased activity of defense mechanisms due to latent damage in these grains. Natural coffees are more sensible to high drying temperatures and its quality reduces faster than pulped coffee in the storage.

Relations between the yield of bean (Phaseolus vulgaris L.) and chemical attributes of an Acrustox under no-tillage

In crop year 2006/07, in Selviria, MS, Brazil, were analyzed the productivity of beans because of the chemical attributes of an Acrustox cultivated under conditions of high technological level of management by no-tillage irrigated with pivot central. The objective was to select, among the attributes studied soil, the one with the best representation to explain the variability of agricultural productivity. Geostatistical grid was installed to collect data from soil and plant, with 117 sampling points in an area of 2,025 m(2) and homogeneous slope of 0.055 m m(-1). From the standpoint of linear and spatial bean yield was respectively explained in terms of P and soil pH. So much for the values of phosphorus (P) in the intermediate layer and subsurface between 24-26 mg dm(-3), as well as for Hydrogen (pH) in the surface layer between 5.0 to 5.4, resulted in sites with the most high yield (2,160-2,665 kg ha(-1)).

Effects of different formulations of neem oil-based products on control Zabrotes subfasciatus (Boheman, 1833) (Coleoptera: Bruchidae) on beans

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)