99 resultados para sincronização
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Medicina Veterinária - FMVZ
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Pós-graduação em Cirurgia Veterinária - FCAV
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The aim of this study was to analyze the effect of flushing on the reproductive performance of Morada Nova (MN) and Santa Inês (SI) ewes submitted to fixed time artificial insemination (TAI). Twenty seven SI and 24 MN supplemented with concentrate (1% of live weight, on average), for 75 days during the breeding season. After 30 days of supplementation, ewes were synchronized with the aid of a hormonal protocol (HP) based on progesterone, eCG and cloprostenol. The estrus observation was conducted at 12, 24, 36 and 48 h after the end of HP with the aid of two ruffians. TAI was done 55 h after the end of HP. From 20 to 45 days after the beginning of the HP ewes were exposed to rams (natural breeding). The pregnancy diagnosis was evaluated 70 days after TAI. We analyzed the weight, body condition score, estrus rate, pregnancy rate and prolificacy testing the effects of race, week of supplementation and body condition score class. The weight and body conditions of ewes varied according to the week of supplementation, with higher values in the first two weeks following TAI. The estrus rate was 88.2% and 43.2% of the ewes showed estrus up to 24 hours of the end of the HP. The pregnancy rate per TAI was 31.3% and the pregnancy rate after natural breeding was 50.0%. It was observed that body condition score classes interfered in pregnancy rates. There was a higher percentage of multiple births by pregnancy by TAI than by natural breeding. It was concluded that the flushing resulted in weight gain and better body conditions ensuring the standardization of the herd for breeding season, which therefore improved reproductive performance. The HP used advanced the onset of estrus and increased prolificacy, but was inefficient in the synchronization of woolless sheep.
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O objetivo deste estudo foi avaliar a eficiência do uso de duas doses de PGF associadas ou não à administração de hCG no início do estro sobre os parâmetros reprodutivos de cabras leiteiras. Um total de 29 cabras receberam duas doses de 30 µg d-cloprostenol pela via latero-vulvar com 10 dias de intervalo (Dia 1 e Dia 10). As cabras foram alocadas para receberem o hCG (250 IU) ou salina i.m. no momento em que o estro foi detectado. Depois da realização da segunda dose de PGF, o estro foi monitorado e exames ultrassonográficos foram realizados duas vezes ao dia. Todas as fêmeas foram inseminadas 16 h após o inicio do estro. Amostras de sangue foram coletadas diariamente para determinação das concentrações plasmáticas de progesterona. O uso do hCG no momento do início do estro não afetou os parâmetros estudados e, portanto, os dados serão apresentados agrupados. A taxa de manifestação de estro foi similar (P > 0,05) na primeira (75,9% - 22/29) ou na segunda dose de PGF (79,3% - 23/29). O intervalo entre a administração de PGF e o início do estro foi maior (P < 0,05) no Dia 1 (75,8±53,9 h) que no Dia 10 (47,7±10,1 h). Duração do estro também diferiu (P < 0,05) 35,4±15,9 (Dia 1) vs 26,8±15, 0 h (Dia 10). A taxa de ovulação foi 79,3% (23/29) após a segunda dose PGF. Não foi encontrada diferença (P>0,05) entre os grupos experimentais quanto aos parâmetros reprodutivos: intervalo entre a aplicação da segunda dose e a ovulação (86,6±11,4h), intervalo do estro a ocorrência da ovulação (39,9±12,3h), diâmetro do maior folículo (7,2±1,4) e número de ovulações (1,8±0,6). No Dia 1, 52,4% (11/21) apresentavam concentrações de progesterona < 1 ng/mL. No Dia 10, 100% dos animais apresentavam concentrações >1ng/mL. O presente estudo permite concluir que o estro pode ser eficientemente sincronizado em cabras leiteiras com duas doses de PGF intervaladas em 10 dias. Novas pesquisas devem se realizadas para avaliar diferentes doses e momentos de utilização do hCG.
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The objective of this study was to assess the reproductive response of adult and prepubertal goats subjected to repeated laparoscopic ovum pick-up (LOPU). The study animals were divided into two groups, specifically, adult nanny goats (GA, n=10) and prepubertal nanny goats (GP, n=10), which were subjected to estrous synchronization and ovarian stimulation for LOPU. Both groups underwent six LOPU procedures at seven-day intervals and were subsequently subjected to controlled mating and pregnancy diagnosis to evaluate their future fertility. The study showed a reduction in the number of follicles visualized and in the amount and quality of the oocytes that were recovered and exposed to in vitro maturation. As indicated by the fertility test, however, no complications were found during the laparoscopic procedures that would impair the reproductive future of the animals. Therefore, a viable number of oocytes were obtained even with the decreased reproductive efficiency, proving that repeated LOPUs do not interfere with the reproductive of adult and prepubertal nanny goats. These results indicate a positive aspect of this procedure, allowing for increasing reproductive performance of this kind, when used for the production in vitro.
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Pós-graduação em Engenharia Elétrica - FEIS
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Germinação de sementes de romäzeiras (Punica granatum L.) de acordo com a concentração de giberelina
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The recombinant bovine somatotropin (rbST) at first, had its widespread use in dairy cows in order to increase milk production. Currently, it has been studied frequently and use their influence both in bovine milk, such as cutting. Its production has been an evolution to the science, using bacteria to produce recombinant DNA. Most authors that have studied and obtained positive results, such as increasing the number of ovarian follicles larger than five millimeters, among others. Its action takes place directly on the ovary, follicles, corpus luteum, the granulosa cells, oviduct, myometrium, endometrium and placenta, where they were found receptors, or indirectly through the release of insuline like growth factor-1 (IGF-1). Therefore, the objective of this work is to explain the importance of bST in bovine as well as the usefulness of this, its mechanism of action and the benefits it can bring when combined with other biotechnology, such as superovulation, embryo transfer, synchronization of estrus, and others
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O presente trabalho apresenta estudos de casos de expressões audiovisuais reunidas por Jan Simons sob a denominação de narrativas complexas, propondo a sua utilização como referência para aplicação de interação na TV Digital. O corpus é constituído das seguintes produções submetidas a essa classificação: forking path narratives e puzzle films com The Butterfly Effect (Erci Bress e J. Mackye Gruber, 2004); mind-game films com Lola Rennt (Tom Tykwer, 1998); modular narratives com Nine Lives (GPS films, 2005), database narratives e modular narratives com Pulp Fiction (Quentin Tarantino, 1994); network narratives com Babel (Alejandro Gonzalez, 2006) e subjectives stories com Being John Malkovich (Spike Jonze, 1999). Compreendida como uma técnica de seleção e articulação de fragmentos de sentidos na composição do discurso audiovisual, aponta-se a montagem como um mecanismo propulsor de interação, manifestada pela transferência da sincronização dos módulos de sentidos que compõe a mensagem ou na construção de discursos audiovisuais com linearidade que se difere do nível dos acontecimentos. A pesquisa gerou um capítulo de livro, duas publicações em revistas científicas, além de integrar anais de diversos eventos acadêmicos
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The aim of the present study was to examine tapping synchronization in children with and without Developmental Coordination Disorder (DCD). Participants were 27 children from which 13 diagnosed with motor difficulties composed the DCD group and 14 children with typical development (TD) the comparison group. The experimental task consisted of performing 25 continuous tapping on a surface of an electronic drum with the preferred hand. Participants were required to tap in synchrony with an auditory bip generated by customized software. Three interval values the tapping task were tested: 470 ms, 1000 ms, 1530 ms. The dependent variables were constant error (CE) and absolute error (AE) and standard deviation of absolute error (SD of AE). The ANOVA 2 x 3 x 3 (Group X Age x Interval) with repeated measures in the last factor for the CE indicated significant interaction among Group X Age X Interval. For the AE and SD of AE the ANOVAs yielded significant main effect of Interval and a significant interaction between Group X Interval. The results of the present study indicated that children with DCD were less accurate and more variable in the tapping synchronization than children with TD. Differences in performance between DCD and children with TD become larger as the interval of the auditory signal increases.
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Pós-graduação em Medicina Veterinária - FCAV