Dinâmica populacional no Sertão do Rio Pardo (1801-1829)

Pós-graduação em História - FCHS

Cryopreservation of bovine spermatozoa from the epididymal tail using conventional and automated methods

Cryopreservation of sperm is important to preserve the gerrnplasm from animals of genetic value, which can die unexpectedly. This study compares conventional and automated methods of cryopreservation of spermatozoa obtained from the epididymis of bulls post-mortem. Twenty-two epididymides were obtained from a commercial slaughterhouse. Spermatozoa were collected from the tail of the epididymis using the retrograde flow technique. Thus, the samples, which were diluted in 10 ml of extender without glycerol (Botubov (R) I, Botupharma, Botucatu, SP, Brazil), were evaluated on motility, sperm vigor, structural and functional (swelling hypoosmotic test) membrane integrity, mitochondrial activity, sperm viability and ADN fragmentation. The samples were divided into two aliquots and diluted in extender with glycerol (Botubov (R) II, Botupharma, Botucatu, SP, Brazil) at a concentration of 50x10(6) motile sperm/0.5 French straws. One sample was frozen by the conventional method (4 hours at 5 degrees C, in a refrigerator and 20 min in nitrogen vapor) and the other by the automated method (Cryogen (R) Dualflex, Neovet, Uberaba, MG, Brazil). The parameters were higher in all the tests of fresh sperm samples, with the exception of the swelling hypoosmotic test, which showed no significant difference when the results were compared with sperm frozen by the conventional method. The average motility of fresh spermatozoa was 74%, and conventional and automated averages were 29 and 25%, respectively. Therefore, although cryopreservation techniques reduce sperm quality parameters, the viability of the sperm is maintained, and these methods can be used to preserve sperm.

Leucoencefalomalácia em equídeos da região Leste de Mato Grosso

Background: Fumonisins produced by Fusarium verticillioides are among the most important medical mycotoxins known. The intake of concentrate based on corn and corn by-products contaminated with fumonisins can cause severe poisoning in horses. The injuries are observed mainly in the white matter of the brain, and the disease is known as Equine Leukoencephalomalacia (ELEM). This study aims to describe and discuss the epidemiological, clinical and diagnostic aspects of an outbreak of ELEM occurred in three farms in the municipalities of Canarana and Agua Boa, in the eastern region of Mato Grosso, Brazil.Materials, Methods & Results: The outbreak occurred between May and August 2010. The disease affected six horses and four mules of different ages and sex. Clinical examination was only possible in animals with chronic evolution of the disease. All the affected animals showed neurological clinical signs such as ataxia and recumbency, which progressed to death or sudden death. Histopathological analysis showed foci of necrosis that predominantly affected the white matter, and the presence of gitter cells. Degenerative lesions were observed in the liver of the animals. Mortality rate ranged from 12.5 to 71%, and lethality reached 100%. The cases were preceded by sudden drops in the weather temperature. Fumonisins levels of 6.6 ppm were detected in the feed of the animals.Discussion: The presumptive diagnosis of leukoencephalomalacia was consistent and based on clinical and epidemiological studies. However, the definitive diagnosis was based upon the histological features of the brain including the presence of extensive areas of malacia. Moreover, the animals were being fed with corn or corn by-products contaminated with fumonisins levels considered to be toxic to equids. The mortality and lethality rates are in agreement with outbreaks described in previous studies. The animals showed neurological signs as the predominant clinical manifestation, with gait ataxia followed by recumbency, prostration and death between 24 h and 29 days. Similarly to other reports, the disease was more frequent in adult animals, which succumbed in 24-48 h. Conversely, the evolution of the disease in young animals was of 10 to 29 days. Sudden death was more prevalent in the mules. Previous studies have shown a predominance of cerebral and brainstem lesions in horses, whereas in mules the clinical signs are related to brainstem lesions. Corn and corn by-products are commonly used as energy supplementation to horses in the southern of Mato Grosso state, but outbreaks of the disease are uncommon. This may be influenced by the prevalence of hot climate conditions, which does not favor the production of toxin by the fungus. The atypical low weather temperatures (9-11 degrees C) observed prior to the outbreak could have contributed to the mycotoxin production by F. verticillioides, which requires temperatures between 8-12 degrees C to produce toxins. However, the disease in the region may be underestimated, considering that the practice of necropsies is not common among field technicians, mainly in the occurrence of sporadic deaths among horses intended for work. Preventive measures include avoiding the use of corn and corn by-products for horses after periods of sudden drops in temperature in the region. Furthermore, clinical and epidemiological surveys and post-mortem and histopathological analyses are undoubtedly important for appropriate differential diagnosis, especially in equids with neurological signs.

Deforming mandibular osteomyelitis in a cow caused by Trueperella pyogenes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phage-displayed peptides as capture antigens in an innovative assay for Taenia saginata-infected cattle

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nematode resistance to ivermectin (630 and 700 mu g/kg) in cattle from the Southeast and South of Brazil

Two high doses of ivermectin (630 mu g/kg and 700 mu g/kg) that are sold commercially in Brazil were evaluated in dose-and-slaughter trials with 144 naturally nematode-infected cattle from eight regions within the states of Minas Gerias, Sao Paulo and Rio Grande do Sul in Brazil. Treatment groups were based on fecal egg counts 1, 2, and 3 days before treatment; all animals studied had a minimum egg count of at least 500 eggs per gram of feces (EPG). Post-mortem analyses were conducted on day 14. The highest levels of resistance to ivermectin were found for Haemonchus placei, Cooperia punctata and Oesophagostomum radiatum; all populations of H. placei were resistant to the 630 mu g/kg dose, and 67% were resistant to 700 mu g/kg; 86% of C. punctata were resistant to the 630 mu g/kg dose, and 33% were resistant to 700 mu g/kg. A combined analysis revealed that 57% of O. radiatum were resistant to the lower dose of ivermectin. H. placei, C. punctata and O. radiatum, in order, were the nematode populations with the highest indices of resistance, whereas Trichostrongylus axei was the most susceptible to 630 and 700 mu g/kg dosages of ivermectin. The results of helminthic resistance to ivermectin for different populations of H. placei and C. punctata described in the present study support previous literature data, in which a small decrease in the average parasitic burden of C. punctata and a consequent increase of H. placei were observed in cattle from the Southeast, South and Center-West regions of Brazil. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

Seleção genômica para características de carcaça em bovinos da raça Nelore

Pós-graduação em Genética e Melhoramento Animal - FCAV

Estudo das células mononucleares e polimorfonucleares no intestino de cães naturalmente infectados por leishmania infantum

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Importância da correta confecção do prontuário odontológico para identificação humana: relato de caso

The clinical records correspond to a set of documents where all information of the patient is stored. When properly confectioned and filed by the dentist, it may serve as a tool for success in dental expertise. The aim of this paper is to present the importance of proper confection of dental records in human identification by means the presentation of a case of identification, occurred in a São Paulo state city. The notes present in the dental records of the alleged victim were very poor, even with a contradiction. However, having endodontic treatment been performed, the comparative analysis between the radiographs of the skull of the victim (postmortem) and the endodontic treatment of tooth 22 (antemortem) permitted to observe total coincidence between the details of such treatment, and anatomical features present in other dental elements. These important parameters of comparison indicated that the body was of the suspect and, due to the number of coincidences, it could not belong to another individual. Nevertheless, the clinical documentation provided was deficient, and presented contradictory data. Because of its fundamental importance for human identification, it is essential that dental professionals take the necessary care to ensure its proper confection and custody, seeking to make the clinical records also an efficient instrument of consultation in identification cases.

Influência da obesidade induzida por dieta hiperlipídica saturada sobre o comportamento da via beta-adrenérgica miocárdica em ratos Wistar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cytopathology in the diagnosis of oral trichomoniasis in a rock pigeon (columba livia)

A Rock Pigeon (Columba livia) submitted for necropsy had an oral white-yellow mass with a friable surface and putrid odor. Impression smears were performed and tissue samples were collected for histopathology. Cytopathological analysis revealed numerous pyriform protozoa, compatible with Trichomonas gallinae. Protozoans were not evident within the lesions by histopathology after staining the samples with Hematoxylin and Eosin or Gomori methenamine silver (GMS) stain. We conclude that impression smears from avian oral trichomoniasis suspects, even during post-mortem evaluation, can be a useful technique for the diagnosis of this disease.

Potencial do cultivo in vitro e do xenotransplante na sobrevida e desenvolvimento de folículos ovarianos pré-antrais em matrizes de ruminantes domésticos em risco de morte: revisão de literatura

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cytopathology in the diagnosis of oral trichomoniasis in a Rock Pigeon (Columba livia)

A Rock Pigeon (Columba livia) submitted for necropsy had an oral white-yellow mass with a friable surface and putrid odor. Impression smears were performed and tissue samples were collected for histopathology. Cytopathological analysis revealed numerous pyriform protozoa, compatible with Trichomonas gallinae. Protozoans were not evident within the lesions by histopathology after staining the samples with Hematoxylin and Eosin or Gomori methenamine silver (GMS) stain. We conclude that impression smears from avian oral trichomoniasis suspects, even during post-mortem evaluation, can be a useful technique for the diagnosis of this disease.

Detecção precoce e quantificação de vírus da cinomose por PCR quantitativa em Tempo Real (qPCR) em diferentes tecidos e fluidos de um cão

A cinomose é uma doença de desafio diagnóstico, especialmente quando não há histórico de vacinação. O objetivo deste estudo foi detectar e quantificar partículas virais de cinomose em diferentes fluidos e tecidos biológicos de um cão, determinando o melhor tecido para diagnóstico viral ante mortem na fase de viremia. Atendeu-se um cão adulto com manifestações clínicas inespecíficas e corpúsculos de Sinegaglia Lentz em linfócitos. Amostras post mortem foram submetidas a PCR em tempo real (qPCR), que demonstrou RNA viral em concentrações de (x105) em líquor (1.216), bexiga (1.009), cérebro (605), sangue (572), cerebelo (523), rins (373), fígado (257), pulmões (191), estômago (154), terceira pálpebra (70) e urina (2,1). A técnica de qPCR permitiu confirmar a infecção pelo vírus, descartando vacinação recente. A amostra de líquor mostrou-se representativa para diagnóstico molecular de fase aguda de cinomose no animal estudado.

Parâmetros oftálmicos em cachorro-do-mato (Cerdocyon thous, Linnaeus, 1766)

Pós-graduação em Cirurgia Veterinária - FCAV