144 resultados para light dark box test
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Influência do fotoperíodo no consumo alimentar, produção e composição do leite de ovelhas Bergamácia
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O objetivo deste trabalho foi avaliar o efeito do fotoperíodo sobre o consumo alimentar, produção e composição do leite (gordura, proteína, lactose, sólidos totais) e número de células somáticas de sete ovelhas da raça Bergamácia, submetidas a um fotoperíodo considerado curto (12 horas luz:12 horas escuro), e oito ovelhas da mesma raça, a um fotoperíodo longo (18 horas luz:6 horas escuro), durante as últimas quatro semanas de gestação e três meses de lactação. Para comparação, foi utilizado o teste t para duas amostras independentes. O consumo alimentar avaliado, diariamente, para cada baia com duas ovelhas, não diferiu entre os tratamentos. No fotoperíodo curto, o consumo médio diário foi de 4,48 kg e, no fotoperíodo longo, de 4,40 kg. Não foi observada diferença significativa entre os fotoperíodos, para a produção de leite e teores de proteína, lactose e sólidos totais, quando considerados os 84 dias de lactação. No fotoperíodo curto, a porcentagem média de gordura foi 5,57%, índice superior ao obtido no fotoperíodo longo, de 5,21%. Na análise dos resultados, a cada semana, notou-se que na segunda e na quarta semanas de lactação, a produção de leite foi maior nas ovelhas do fotoperíodo longo. O número de células somáticas não diferiu entre os fotoperíodos e manteve-se baixo por toda a lactação. O maior número de horas de luz estimula a produção de leite no primeiro mês de lactação, principalmente na quarta semana, e as porcentagens de gordura e sólidos totais são menores nos períodos de maior produção de leite.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Purpose: To evaluate the bond strength between human dentin and composites, using two light-activated single-bottle total-etch adhesive systems with different pHs combined with chemically activated and light-activated-composites. The tested hypothesis was that the dentin bond strength is not influenced by an adhesive system of low pH, combined with chemically activated or light-activated composites. Material and Method: Flat dentin surfaces of twenty-eight human third molars were allocated in 4 groups (n=7), depending on the adhesive system: (One Step Plus-OS and Prime & Bond NT-PB) and composite (light-activated Filtek Z-100 [Z100] and chemically activated Bisfil 2B [B2B]). Each adhesive system was applied on acid-etched dentin and then one of the composites was added to form a 5 mm-high resin block. The specimens were stored in tap water (37 degrees C/24 h) and sectioned into two axes, x and y. This was done with a diamond disk under coolant irrigation to obtain beams with a cross-section area of approximately 0.8 mm(2). Each specimen was then attached to a custom-made device and submitted to the microtensile test (1 mm.min(-1)). Data were analyzed using two-way ANOVA and Tukey's tests (p<0.05). Results: the anticipated hypothesis was not confirmed (p<0.0001). The bond strengths (MPa) were not statistically different between the two adhesive systems when light-activated composite was used (OS+Z100 = 24.7 +/- 7.1(a); PB+Z100 = 23.8 +/- 5.7(a)). However, with use of the chemically activated composite (B2B), PB (7.8 +/- 3.6(b) MPa) showed significantly lower dentin bond strengths than OS (32.2 +/- 7.6(a)). Conclusion: the low pH of the adhesive system can affect the bond of chemically activated composite to dentin. on the other hand, under the present conditions, the low pH did not seem to affect the bond of light-activated composites to dentin significantly.
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Cucumis anguria present dark germinating seeds at 25 degrees C. Seeds without the tegument are photosensitive germinating better in darkness than under continuous white light. However, pre-incubation at 40 degrees C for 48 hours allows the seeds to germinate under continuous white light and the incubation of naked seeds at -0.6MPa restored the light inhibition of seed germination. Our results suggest that the tegument interact with the phytochrome in the control of seed germination in part of the population of seed of C. anguria.
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This study evaluated the Knoop hardness of one resin cement (dual-cure mode or light-cure mode) when illuminated directly or through restorative materials-ceramic (HeraCeram) or composite (Artglass)-by two light curing units. Light curing was carried out using a conventional quartz tungsten halogen (QTH) light source (XL2500) for 40 s, and a light emitting diodes (LED) light source (Ultrablue Is) for 40 s. Bovine incisors had their buccal faces flattened and hybridised. on these surfaces, a mould was seated and filled with cement. A disc of the veneering material (1.5 mm thickness) was positioned over this set for light curing. After storage (24 h/37 degrees C), samples (n = 10) were sectioned for hardness (KHN) measurements. Data were submitted to ANOVA and to Tukey's test (alpha = 0.05). In general, light curing with LED resulted in higher hardness values than QTH. Distinct cement behaviour was observed with different veneering material in association with different light curing units (LCUs). (C) 2006 Elsevier Ltd. All rights reserved.
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Photosynthetic characteristics in response to irradiance were analysed in 21 field and culture populations of thirteen freshwater red algal species applying two distinct techniques (chlorophyll fluorescence and oxygen evolution). Photosynthesis-irradiance (PI) curves indicated adaptations to low irradiances in all species analysed, essentially characterized by occurrence of photoinhibition, low values of the saturation parameter (I-k < 225 mu mol m(-2) s(-1)) and compensation irradiance (I-c < 20 mu mol m(-2) s(-1)) and relatively high values of the effective quantum yield of photosystem II (Delta F/F'(m) >= 45). These characteristics have been reported in freshwater red algae and were confirmed from data based on the two techniques, indicating they are typically shade-adapted plants. on the other hand, some species (e.g. Batrachospermum delicatulum) can tolerate high irradiances (up to 2400 mu mol m(-2) s(-1)), suggesting they have mechanisms that enable them to avoid photodarnage of the photosynthetic apparatus. One of these mechanisms is the increase in dissipation of excessive energy captured by reaction centres after exposure to continuous irradiance, as reflected by the non-photochemical quenching fluorescence parameter in dark/light induction curves. Photo-inhibition occurred in all algae tested by both techniques. Light acclimation was evident particularly in field populations, as revealed by lower values of the saturation parameter (Ik) and the compensation irradiance (I-c) and higher values of Delta F/F'(m) in algae under low irradiances (shaded or heavily shaded stream segments), and vice-versa. Forms living within the boundary layer (e.g. crusts), in a region of reduced current velocity, tended to be more shade-adapted than semi-erect plants (e.g. non-mucilaginous or mucilaginous filaments), as indicated by highest values of photosynthetic efficiency (alpha = 0.31) and effective quantum yield (Delta F/F'(m) = 0.88) under natural conditions. Higher photo- synthetic rates (P-max) for the same species or population were observed under culture than field conditions when measured with the oxygen evolution technique, whereas the opposite trend was observed using chlorophyll fluorescence. (c) 2005 Elsevier B.V. All rights reserved.
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The aim of this study was to evaluate the effects of the laser radiation (685 nm) associated with photosensitizers on viability of different species of Candida genus. Suspensions of Candida albicans, Candida dubliniensis, Candida krusei and Candida tropicalis, containing 106 viable cells per milliliter were obtained with the aid of a Neubauer's chamber. From each species, 10 samples of the cell suspension were irradiated with diode laser (685 nm) with 28 J/cm(2) in the presence of methylene blue (0.1 mg/ml), 10 samples were only treated with methylene blue, 10 samples were irradiated with laser in the absence of the dye, 10 samples were treated with the dye and irradiated with laser light and 10 samples were exposed to neither the laser light nor to the methylene blue dye. From each sample, serial dilutions of 10(-2) and 10(-3) were obtained and aliquots of 0.1 ml of each dilution were plated in duplicate on Sabouraud dextrose agar. After incubation at 37 degrees C for 48 h, the number of colony-forming units (CFU/ml) was obtained and data were submitted to ANOVA and Tukey's test (p < 0.05). Laser radiation in the presence of methylene blue reduced the number of CFU/ml in 88.6% for C. albicans, 84.8% for C. dubliniensis, 91.6% for C krusei and 82.3% for C tropicalis. Despite of this, only laser radiation or methylene blue did not reduce significantly the number of CFU/ml of Candida samples, except for C tropicalis. It could be concluded that the photo activation of methylene blue by the red laser radiation at 685 nm presented fungicide effect on all Candida species studied. (c) 2006 Elsevier B.V. All rights reserved.
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We tested the effects of monochromatic light on the specific growth rate (SGR), feed intake and feed efficiency (FE) of juvenile pikeperch, Sander lucioperca (L.). Pikeperch were reared individually for 42 days in aquaria covered with blue, green, yellow or red gelatin filters or white paper (control; n=5). Linear regression analysis indicated a significant positive effect of longer wavelengths of light on the condition factor (CF), FE and SGR. The final weight, SGR and CF were significantly higher in fish reared under red than under white light, and FE was better under green, yellow and red light than under white light (Dunnett's post hoc test, P < 0.05) while blue was comparable to white light in terms of the measured parameters. After the growth trial, the spectral sensitivity of photoreceptor cells in the retina was tested using microspectrophotometry, which revealed the presence of rods with lambda(max) at ca. 530 nm and two cone classes, absorbing maximally at ca. 535 and 603 nm, all containing a porphyropsin-based pigment. These results suggest that the presence of mid and long wavelength-sensitive cones enhances visual sensitivity under mid-wavelength and long-wavelength environments, and thus supports the finding that longer wavelengths of incoming light can improve FE and SGR of the cultivated pikeperch.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Monochromatic light excitation in conjunction with thermally stimulated depolarization current measurements are applied to indirect bandgap AlxGa1-xAs. The obtained average activation energy for dipole relaxation is in very close agreement with the DX center binding energy. Monochromatic light induces state transition in the defect and makes possible the identification of dipoles observed in the dark. Charge relaxation currents are destroyed by photoionization of Al0.5Ga0.5As using either 647 nm Kr+ or 488 nm Ar+ laser lines, which are above the DX center threshold photoionization energy. It suggests that correlation may exist among charged donor states DX--d+. Sample resistance as a function of temperature is also measured in the dark and under illumination and shows the probable X valley effective mass state participation in the electron trapping. Ionization with energies of 0.8 eV and 1.24 eV leads to striking current peak shifts in the thermally stimulated depolarization bands. Since vacancies are present in this material, they may be responsible for the secondary band observed in the dark as well as participation in the light induced recombination process.
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The present assay had as objective evaluating spore germination of Blechnum brasiliense in relation to light, plant density and disinfection. The assay was carried out at Jaboticabal, Sào Paulo State, Brazil, from February, 22 to June, 30, 1996. The experimental design was randomized blocks on a factorial scheme (3x2x2), consisting of 12 treatments, three environments (shade-house, dark-house and germination camera), 2 densities (0.005 grs and 0.010 grs of spores/treatment) and presence or absence of disinfection. The leaf coverage area (130 days) and the number of days necessary to germinate were evaluated. The germination camera data were not analysed because they were insignificant; consequently, the remining data were analysed on a 2×2×2 scheme. The shade-house provided larger green covering area and a faster germination. The density of 0.0 lOg of spore/treatments presented the largest green covering area. The supply of partial light was necessary for good germination. The interaction between the environment and the density had significant effect on the green covering area.
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We apply the negative dimensional integration method (NDIM) to three outstanding gauges: Feynman, light-cone, and Coulomb gauges. Our aim is to show that NDIM is a very suitable technique to deal with loop integrals, regardless of which gauge choice that originated them. In the Feynman gauge we perform scalar two-loop four-point massless integrals; in the light-cone gauge we calculate scalar two-loop integrals contributing to two-point functions without any kind of prescriptions, since NDIM can abandon such devices - this calculation is the first test of our prescriptionless method beyond one-loop order; and finally, for the Coulomb gauge we consider a four-propagator massless loop integral, in the split-dimensional regularization context. © 2001 Academic Press.