Diagnosis of Giardia infections by PCR-based methods in children of an endemic area

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Moxidectin-resistant nematodes in cattle in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Environmental factors influencing the transmission of Haemonchus contortus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Molecular screening of Plasmodium sp. asymptomatic carriers among transfusion centers from Brazilian Amazon region

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Primeiro registro de predação de Poecilia reticulata Peters, 1859 e Phalloceros caudimaculatus (Hensel, 1868) por Noctilio leporinus (Linnaeus, 1758) (Chiroptera, Noctilionidae)

Noctilio leporinus (Chiroptera, Noctilionidae) has adaptations to piscivorous feeding habits, but also consumes crustaceans and insects. Between June 2005 and May 2006, bats were surveyed with mist nets during two evenings per month in the gallery forest of Corrego dos Tenentes, Pindorama, São Paulo State, Brazil. The objectives were to collect fecal samples and identify the fish species that were consumed by N. leporinus in this fragmented forest landscape. We documented two new fish species to its diet, the guppy Poecilia reticulata, Peters, 1859 and the "guaru" Phalloceros caudimaculatus (Hensel, 1868). Both fish species forage near the surface of water for insects and zooplankton, which is an area also utilized by N. leporinus when foraging for insects, crustaceans and fishs.

Screen-Printed Carbon Electrode Modified with Poly-L-histidine Applied to Gold(III) Determination

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Development of a potentiometric flufenamate ISE and its application to pharmaceutical and clinical analyses

The characteristics, performance, and application of an electrode, namely, Pt vertical bar Hg vertical bar Hg-2(FF)(2)vertical bar Graphite, where FF stands for flufenamate ion, are described. This electrode responds to FF with sensitivity of (-58.6 +/- 1.2) mV decade(-1) over the range 1.0 x 10(-6) - 1.0 x 10(-2) mol L-1 at pH 6.0-9.0 and a detection limit of 7.1 x 10(-7) mol L-1. The electrode is easily constructed at a relatively low cost with fast response time (within 10-25 s) and can be used for a period of 5 months without any considerable change in its performance characteristics. The proposed electrode displayed good selectivity for flufenamate in the presence of several substances as well as in the presence of some carboxylate and inorganic anions. The electrode was successfully applied to the determination of flufenamic acid in pharmaceuticals and human serum samples.

Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Frugivoria em morcegos (Mammalia, Chiroptera) no Parque Estadual Intervales, sudeste do Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diagnóstico de criptosporidiose em amostras fecais de bezerros por imunofluorescência direta e microscopia de contraste de fase

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Contaminação de praças públicas de Campo Grande, Mato Grosso do Sul, Brasil, por ovos de Toxocara e Ancylostoma em fezes de cães

De fevereiro a outubro de 1998, avaliou-se a contaminação de praças públicas de Campo Grande, MS, Brasil, por ovos de Toxocara e Ancylostoma, em amostras de fezes de cães. Das 74 praças examinadas, 42 (56,8%) estavam contaminadas por ovos de Ancylostoma, 8 (10,8%) com ovos de Toxocara e 7 (9,5%) com ambos.

Fezes de galinhas poedeiras na alimentação de ruminantes análises microbiológicas

The objectives of the present study were: to evaluate the variation in total number of microorganisms in laying hen fecal samples and to identify the pathogenic microorganisms possibly present such as Staphylococcus aureus, Salmonella sp., Clostridium botulinum, C chauvoei, Campylobacter sp., Escherichia coli, and Corynebacterium sp. The experiment was conducted at the School of Agrarian and Veterinarian Sciences of Jaboticabal, - UNESP. Fecal samples were collected from the aviary. A fully randomized experimental design was used with 7 treatments and 4 replications, for a total of 28 samples. A decrease in total number of microorganisms was detected when treatment T0 (zero days of storage) was compared with treatment T6 (42 days of storage), with oscillations in numbers being observed during this period of time. Pathogenic bacteria such as Clostridium noyvi, C perfringens, C sordelli, C septicum and C noyvi type B were detected, as well as non-pathogenic bacteria such as Bacillus sp. and Staphylococcus epidermidis.

A Simple Method Based on ICP-MS for Estimation of Background Levels of Arsenic, Cadmium, Copper, Manganese, Nickel, Lead, and Selenium in Blood of the Brazilian Population

Throughout the world, biomonitoring has become the standard for assessing exposure of individuals to toxic elements as well as for responding to serious environmental public health problems. However, extensive biomonitoring surveys require rapid and simple analytical methods. Thus, a simple and high-throughput method is proposed for the determination of arsenic (As), cadmium (Cd), copper (Cu), manganese (Mn), nickel (Ni), lead (Pb), and selenium (Se) in blood samples by using inductively coupled plasma-mass spectrometry (ICP-MS). Prior to analysis, 200 l of blood samples was mixed with 500 l of 10% v/v tetramethylammonium hydroxide (TMAH) solution, incubated for 10 min, and subsequently diluted to 10 ml with a solution containing 0.05% w/v ethylenediamine tetraacetic acid (EDTA) + 0.005% v/v Triton X-100. After that, samples were directly analyzed by ICP-MS (ELAN DRC II). Rhodium was selected as an internal standard with matrix-matching calibration. Method detection limits were 0.08, 0.04, 0.5, 0.09, 0.12, 0.04, and 0.1 g//L for As, Cd, Cu, Mn, Ni, Pb, and Se, respectively. Validation data are provided based on the analysis of blood samples from the trace elements inter-\comparison program operated by the Institut National de Sante Publique du Quebec, Canada. Additional validation was provided by the analysis of human blood samples by the proposed method and by using electrothermal atomic absorption spectrometry (ETAAS). The method was subsequently applied for the estimation of background metal blood values in the Brazilian population. In general, the mean concentrations of As, Cd, Cu, Mn, Ni, Pb, and Se in blood were 1.1, 0.4, 890, 9.6, 2.1, 65.4, and 89.3 g/L, respectively, and are in agreement with other global populations. Influences of age, gender, smoking habits, alcohol consumption, and geographical variation on the values were also considered. Smoking habits influenced the levels of Cd in blood. The levels of Cu, Mn, and Pb were significantly correlated with gender, whereas Cu and Pb were significantly correlated with age. There were also interesting differences in Mn and Se levels in the population living in the north of Brazil compared to the south.

Investigação de parasitoses e/ou comensais intestinais em manipuladores de alimentos de escolas públicas

The objective of this work was to identify intestinal parasites and/or commensals among food handlers of public schools in the city of Patos of Minas (MG). The fecal samples were analyzed by Lutz's method (1919). The positive results were 21.7% (13 cases) for parasites and/or commensal. The frequence of intestinal parasites and/or commensal of food handlers in the public schools showed low positive, probably as a result of good habits of personal hygiene and prophylactic treatment of parasitic.