Immunohistochemistry as a fundamental tool for the differential diagnosis of polymorphous low-grade adenocarcinoma

Polymorphous low-grade adenocarcinoma (PLGA) is a subtype of salivary gland carcinoma with a marked predilection for the minor salivary glands. The diagnosis of this lesion can be a challenge in incisional biopsies because of its similarity to other salivary neoplasms and the difficulty of analyzing its infiltrative nature and neurotropism. Diagnosis can be facilitated, however, by immunohistochemistry, a technique that has become a fundamental tool to the pathologist. The purpose of this study was analyze the immunohistochemical aspects of 4 cases that were examined in the São José dos Campos School of Dentistry. The patients included 4 women, ages 32 to 57 years (mean, 50 years), who reported an intraoral painless lesion, ranging in size from 1 to 3 cm (mean, 1.75 cm) with a variable evolution period. Histologically, the lesions showed similar aspects, characterized by polymorphous growth pattern, including solid, cribriform, trabecular, papillary, and single-file arrays. The tumors showed positivity to cytokeratins (Cks) 7 and 8 and vimentin, variable expression for Ck 14, and a negative reaction to Cks 13 and 19 and muscle-specific actin. PLGA is a tumor of the salivary gland that must be distinguished from other neoplasms for therapeutic and prognostic considerations, and the panel of antibodies employed in this study was effective for its diagnosis.

Composição e digestibilidade in vitro de genótipos de capim-elefante, aos 56 dias de rebrota

Twelve elephant grass genotypes were evaluated in a randomized blocks design with three repetitions. The experimental parcel was of 4 lines with 3m of length, spaced of 1 m and fertilized with 100 kg of P2O5, 100 kg of N, 60 kg of K2O and 25 kg of micronutrients/ha. Significant differences were observed among the genotypes for neutral detergent fiber (FND), acid detergent fiber (FAD) and lignin. The genotypes CNPGL 92-70-02, CNPGL 91-25-01 and CNPGL 93-32-02 showed low values for FND, FAD and lignin, simultaneously, outstanding between the others. There were no significant differences in the levels of dry matter and crude protein contents and in in vitro dry matter digestibility between the genotypes.

Efeito de níveis de fósforo não-fítico e de fitase sobre a tíbia de frangos de corte

It was evaluated the effect of nonphytate phosphorus (NNP) and phytase on broiler tibia. One thousand and two hundred chicks were allocated in a completely randomized design and factorial arrangement 4 x 3 (NPP x phytase levels), with four replicates. NPP levels, at each phase, were 0.45, 0.37, 0.29 and 0.21% - initial, 0.41, 0.33, 0.25 and 0.17% - growth, and 0.37, 0.29, 0.21 and 0.13% - withdrawal. Phytase levels were 0, 500 and 1000 FTU/kg of diet. There was no effect on diameter at 21 days of age. Tibia with lower weight, length and diaphysis, distal epiphysis and average densities e proximal epiphysis density resulted from NPP reduction from 0.37 to 0.29% and from 0.29 to 0.21%, respectively. Inclusion of 500 FTU/kg of phytase improved length and diaphysis density of the tibia, independent of NPP levels. Diets with NPP, at initial, growth and final phases, of 0.37, 0.33 and 0.29%, respectively, supplemented with 1000 FTU/kg of phytase, can be used with no negative effect on bone quality in broilers.

Entropy variation in isothermal fluid flow considering real gas effects

The present paper concerns on the estimative of the pressure loss and entropy variation in an isothermal fluid flow, considering real gas effects. The 1D formulation is based on the isothermal compressibility module and on the thermal expansion coefficient in order to be applicable for both gas and liquid as pure substances. It is emphasized on the simple methodology description, which establishes a relationship between the formulation adopted for ideal gas and another considering real gas effects. A computational procedure has been developed, which can be used to determine the flow properties in duct with a variable area, where real gas behavior is significant. In order to obtain quantitative results, three virial coefficients for Helium equation of state are employed to determine the percentage difference in pressure and entropy obtained from different formulations. Results are presented graphically in the form of real gas correction factors, which can be applied to perfect gas calculations.

Extração de vias combinando métodos de detecção de regiões e linhas em imagens de intensidade de pulso laser

This paper aims at extracting street centerlines from previously isolated street regions by using the image of laser scanning intensity. In this image, streets are easily identified, since they manifest as dark, elongate ribbons contrasting with background objects. The intensity image is segmented by using the region growing technique, which generates regions representing the streets. From these regions, the street centerlines are extracted in two manners. The first one is through the Steger lines detection method combined with a line length thresholding by which lines being shorter than a minimum length are removed. The other manner is by combining the skeletonization method of regions based on the Medial Axis Transform and with a pruning process to eliminate as much as possible the ramifications. Experiments showed that the Steger-based method provided results better than the method based on skeletonization.

Determination of 2-methylimidazole and 4-methylimidazole in caramel colors by capillary electrophoresis

The use of chemical preservative compounds is common in the food products industry. Caramel color is the most usual additive used in beverages, desserts, and breads worldwide. During its fabrication process, 2- and 4-methylimidazole (MeI), highly carcinogenic compounds, are generated. In these cases, the development of reliable analytical methods for the monitoring of undesirable compounds is necessary. The primary procedure for the analysis of 2- and 4-MeI is using LC- or GC-MS techniques. These procedures are time-consuming and require large amounts of organic solvents and several pretreatment steps. This prevents the routine use of this procedure. This paper describes a rapid, efficient, and simple method using capillary electrophoresis (CE) for the separation and determination of 2- and 4-MeI in caramel colors. The analyses were performed using a 75 μm i.d. uncoated fused-silica capillary with an effective length of 40 cm and a running electrolyte consisting of 160 mmol L-1 phosphate plus 30% acetonitrile. The pH was adjusted to 2.5 with triethylamine. The analytes were separated within 6 min at a voltage of 20 kV. Method validation revealed good repeatability of both migration time (<0.8% RSD) and peak area (<2% RSD). Analytical curves for 2- and 4-MeI were linear in the 0.4-40 mg L-1 concentration interval. Detection limits were 0.16 mg L-1 for 4-MeI and 0.22 mg L-1 for 2-MeI. The extraction recoveries were satisfactory. The developed method showed many advantages when compared to the previously used method. © 2013 American Chemical Society.

Efeitos da experiência no andar na organização da passada durante a ultrapassagem sobre obstáculos em bebês

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo conformacional de polianfóteros fracos utilizando simulações de Monte Carlo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da paralisação escalonada da aeração na eficiência do tratamento aeróbio na manipueira em batelada

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Consumo de suplementos esportivos e composição corporal de adolescentes praticantes de exercícios físicos em academias

Pós-graduação em Alimentos e Nutrição - FCFAR

Análise morfológica da superfície dos cones de guta-percha submetidos à desinfecção com hipoclirito de sódio a 1% e a 2,5% e sua influência no selamento marginal da obturação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização morfológica e citogenética de sementes e plântulas de algumas espécies de plantas tóxicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do plano de desenvolvimento e proteção ambiental da Bacia Hidrográfica do Guarapiranga em consonância com o planejamento urbano

Pós-graduação em Geociências e Meio Ambiente - IGCE

Adubação orgânica e biodinâmica na produção de chicória (cichorium endivia) e de beterraba (beta vulgaris), em sucessão

Pós-graduação em Agronomia (Horticultura) - FCA

Distribuição do sistema radicular e produtividade de cana-de-açúcar (Saccharum spp.) fertirrigada por gotejamento subsuperficial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)