556 resultados para bactérias oportunistas


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Pós-graduação em Microbiologia Agropecuária - FCAV

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Pós-graduação em Microbiologia Agropecuária - FCAV

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Microbiologia Agropecuária - FCAV

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The objective of this work was to evaluate the effects of the inoculation with plant growth-promoting bacteria on the formation of sugarcane pre-sprouted seedlings, originated from individual buds, and to quantify the initial growth of these seedlings in association with nitrogen application, in a low fertility soil. Two experiments were carried out: one in greenhouse conditions, with a time span of 50 days, and the other in vases under field conditions, with a time span of 180 days. In both experiments, a randomized complete block design was used with a factorial arrangement of 2x3, in the first experiment - with or without inoculation, and with three quantities of bud reserve -, and of 2x2x4, in the second one - with or without inoculation, with or without nitrogen, evaluated in four times: at 45, 90, 135, and 180 days. The inoculant had effect on the initial growth of the pre-sprouted seedlings, increasing sprouting speed and dry matter accumulation on roots and shoots, regardless of the bud reserve amount. In the second experiment, the inoculant increased the initial growth of shoots and root system up to 180 days after transplantation, increasing height, tillering, stalk diameter, dry matter production of stalks and straw, and root length, regardless of nitrogen application. The inoculant has a positive physiological effect on plant growth.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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A utilização de compostos naturais na medicina de populações tradicionais é uma realidade em todo o mundo e o cerrado brasileiro apresenta grande diversidade de vegetais com uso potencial no tratamento de infecções por microrganismos oportunistas. Esse estudo avaliou a atividade antimicrobiana dos extratos vegetais do cerrado brasileiro sobre microrganismos oportunistas e superinfectantes. Nos testes foram preparados extratos hidroalcoólicos e aquosos de 22 espécies de plantas utilizadas nas regiões Norte, Nordeste e Centro-Oeste como parte da medicina popular. Esses extratos foram testados sobre Enterococcus faecalis ATCC 19433 e Pseudomonas aeruginosa ATCC 10145 e dez isolados clínicos de cada uma dessas espécies microbianas. Inicialmente foi realizada uma triagem para se determinar a atividade antimicrobiana dos extratos. Em seguida, foram realizados testes para avaliar a maior diluição inibitória dos extratos por meio do método de diluição em caldo e testes de atividade inibitória em biofilme. Os resultados demonstraram que embora aproximadamente 20% de todos os extratos mostraram atividade inibitória sobre algum dos microrganismos alvo, os mais ativos foram os extratos aquosos e hidroalcoólicos de araçá e aroeira, se mostrando ativos frente a todos os microrganismos.

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The relationship between the occurrence of enterococci in the oral microbiota and serious infections in patients hospitalized in intensive care units (ICU) has been established. This study evaluated the presence of Enterococcus faecalis and other species of this genus in the mouths of patients on ICU, correlating it with oral and systemic conditions. Data on health and socioeconomic, medication use, medical and family history of patients maintained for 72 hours in the ICU, diagnosed with severe infection or who have developed this condition after the entry to the unit were obtained. Fifty patients provided intraoral and extraoral clinical samples for analysis (above and subgingival biofilm, saliva and buccal mucosa, followed by obtaining samples of respiratory secretions for patients with pneumonia, and blood and urine for sepsis). The presence of target microorganisms was performed by polymerase chain reaction (PCR) and culture using selective media. The chi-square and Mann-Whitney tests were used for statistical analysis, and the significance level was 5%. The intraoral clinical conditions of the patients showed poor. E. faecalis was significantly more frequent microorganism, followed by E. faecium. The use of broadspectrum antimicrobial action was associated with the presence of these opportunistic microorganisms. These bacteria were more frequent in patients with periodontitis or gingivitis. The results showed that enterococci associated with serious infectious processes may originate from resident microbiota of patients and its prevalence is not elevated in healthy individuals.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)