Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817

This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads). The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil. Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive). The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids). Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli. In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules. The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells. The bound calcium was mainly found in the F and M cells. The F cells showed strong positive staining for lipid while the R cell only stained weakly. The E cells did not react to any of the applied staining techniques. The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis. Also, they may be more related to the molt stage, as in the case of calcium salts. © 2013 Elsevier GmbH. All rights reserved.

Uso da técnica de difusão em filmes finos por gradientes de concentração (DGT) para determinação in situ de metais-traço nos rios da Bacia Amazônica Central

Pós-graduação em Geociências e Meio Ambiente - IGCE

Metallomic study of mercury in fish from Amazon region - Brazil

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish from Amazonas region - Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and subsequent evaluation of mercury by Thermal Decomposition and Amalgamation Coupled with Atomic Absorption (TTA-CAAS). It was found that mercury is present in 18 protein spots of dourada muscle (Brachyplatystoma rousseauxii) and 9 protein spots of pacu muscle (Myleus sp.). The protein spots in which they were determined the presence of mercury present molecular weight of 13.5 and 21.4 kDa and isoelectric point of 3.8 and 9.2, respectively.

Cassava wastewater as carbon source for the polyhydroxybutyrate production by Azotobacter vinelandii CCT 2841

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fracionamento geoquímico de metais em sedimentos e avaliação da qualidade da água do Rio Bento Gomes, Pantanal de Poconé, Mato Grosso

Pós-graduação em Química - IQ

Constructed Wetland for Treating Effluent from Subtropical Aquaculture Farm

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sílica mesoporosa como suporte sólido para o ancoramento da molécula 4-amino-3-hidrazino-5-mercapto-1,2,4-triazole e aplicação na adsorção de Cu(II), Cd(II), Ni(II), Pb(II) e Co(II) em amostras aquosas

Pós-graduação em Química - IQ