109 resultados para Sucre, Antonio José de, 1795-1830.
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Considering there are few researches about the influence of seedlings fertilization on yield, this work was carried out in order to evaluate the effect of rates of nitrogen and potassium, applied by fertirrigation, in endive seedlings on yield. Two experiments were carried out, the first with four treatments, varying nitrogen rates, and the second with four potassium rates. In both, experimental design was randomized blocks, with four replications. In the first experiment, we utilized ammonium nitrate, weekly, at rates 0; 40; 80 and 120 mg of N L-1, while in the second experiment we utilized potassium chloride, weekly, at rates 0; 70; 140 and 210 mg of K2O L-1. The evaluated characteristics at seedling stage were shoot height, number of leaves, fresh and dry shoot weight. At harvesting, we evaluated plant height, diameter, number of leaves and fresh weight. Potassium rates did not influence all characteristics, at seedling and at harvesting stages. For all seedling characteristics, there was linear increasing depending on nitrogen rates. But, for plant fresh weight, diameter and number of leaves at harvesting, the effect was quadratic. The highest fresh weight and number of leaves at harvesting were obtained with 90 mg of N L-1.
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Pós-graduação em Artes - IA
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The aim of the present study was to evaluate the efficacy of peroxidase immobilized on corncob powder for the discoloration of dye. Peroxidase was extracted from soybean seed coat, followed by amination of the surface of the tertiary structure. The aminated peroxidase was immobilized on highly activated corncob powder and employed for the discoloration of bromophenol blue. Amination was performed with 10 or 50 mmol.L-1 carbodiimide and 1 mol.L-1 ethylenediamine. The amount of protein in the extract was 0.235 ± 0.011 mg.mL-1 and specific peroxidase activity was 86.06 ± 1.52 µmol min-1 . mg-1, using 1 mmol.L-1 ABTS as substrate. Ten mmol.L-1 and 50 mmol.L-1 aminated peroxidase retained 88 and 100% of the initial activity. Following covalent immobilization on a corncob powder-glyoxyl support, 10 and 50 mmol.L-1 aminated peroxidase retained 74 and 86% of activity, respectively. Derivatives were used for the discoloration of 0.02 mmol.L-1 bromophenol blue solution. After 30 min, 93 and 89% discoloration was achieved with the 10 mmol.L-1 and 50 mmol.L-1 derivatives, respectively. Moreover, these derivatives retained 60% of the catalytic properties when used three times. Peroxidase extracted from soybean seed coat immobilized on a low-cost corncob powder support exhibited improved thermal stability.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Background: Pectus excavatum is characterized by a depression of the anterior chest wall (sternum and lower costal cartilages) and is the most frequently occurring chest wall deformity. The prevalence ranges from 6.28 to 12 cases per 1000 around the world. Generally pectus excavatum is present at birth or is identified after a few weeks or months; however, sometimes it becomes evident only at puberty. The consequence of the condition on a individual's life is variable, some live a normal life and others have physical and psychological symptoms such as: precordial pain after exercises; impairments of pulmonary and cardiac function; shyness and social isolation. For many years, sub-perichondrial resection of the costal cartilages, with or without transverse cuneiform osteotomy of the sternum and placement of a substernal support, called conventional surgery, was the most accepted option for surgical repair of these patients. From 1997 a new surgical repair called, minimally invasive surgery, became available. This less invasive surgical option consists of the retrosternal placement of a curved metal bar, without resections of the costal cartilages or sternum osteotomy, and is performed by videothoracoscopy. However, many aspects that relate to the benefits and harms of both techniques have not been defined. Objectives: To evaluate the effectiveness and safety of the conventional surgery compared with minimally invasive surgery for treating people with pectus excavatum. Search methods: With the aim of increasing the sensitivity of the search strategy we used only terms related to the individual's condition (pectus excavatum); terms related to the interventions, outcomes and types of studies were not included. We searched the Cochrane Central Register of Controlled Trials (CENTRAL), PubMed, Embase, LILACS, and ICTPR. Additionally we searched yet reference lists of articles and conference proceedings. All searches were done without language restriction. Date of the most recent searches: 14 January 2014. Selection criteria: We considered randomized or quasi-randomized controlled trials that compared traditional surgery with minimally invasive surgery for treating pectus excavatum. Data collection and analysis: Two review authors independently assessed the eligibility of the trials identified and agreed trial eligibility after a consensus meeting. The authors also assessed the risk of bias of the eligible trials. Main results: Initially we located 4111 trials from the electronic searches and two further trials from other resources. All trials were added into reference management software and the duplicates were excluded, leaving 2517 studies. The titles and abstracts of these 2517 studies were independently analyzed by two authors and finally eight trials were selected for full text analysis, after which they were all excluded, as they did not fulfil the inclusion criteria. Authors' conclusions: There is no evidence from randomized controlled trials to conclude what is the best surgical option to treat people with pectus excavatum.
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The human enzyme dihydroorotate dehydrogenase (HsDHODH) has been studied for being a target for development of new antineoplasic and antiproliferative drugs. The synthetic peptide N-t(DH) represents the N-terminal microdomain of this enzyme, responsible for anchoring it to the inner mitochondrial membrane. Also, it is known to harbor quinones that are essential for enzyme catalysis. Here we report structural features of the peptide/membrane interactions obtained by using CD and DEER spectroscopic techniques, both in micelles and in lipid vesicles. The data revealed different peptide conformational states in micelles and liposomes, which could suggest that this microdomain acts in specific regions or areas of the mitochondria, which can be related with the control of the quinone access to the HsDHODH active site. This is the first study to report on conformational changes of the HsDHODH N-terminal microdomain through a combination of CD and DEER spectroscopic techniques.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The purpose of this study is to characterize the geological subsurface structure of the diabase sill of the Paraná Magmatic Province intruded in the Itararé Group, located near the city of Limeira, in the east-central region of São Paulo state. The magnetometric method served as an important tool defining the igneous body in view of the great contrast between the physical properties of the diabase sill and the host sandstones. Previous cartographic data signaled in region a possible lateral continuity in subsurface, of the various sills that occur in the region. However, the magnetic data obtained herein indicate that the area of occurrence of this body is very restricted and independent of the others observed in the region. The analysis of the radial average power spectrum obtained allowed determine the interval of number of waves adequate for the separation of shallow and deep sources, and thus conclude that the sill of the Limeira region is located more than 100 m deep.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Mulberry leaves (IZ 56/4 cultivar) in the pos-harvest, had been taken to the laboratory and submitted to six storage environments (8 hours in open ambient-O, 8 hours in cover ambientC, 1hO/7hC, 2hO/6hC, 3hO/5hC and 4hO/4hC), aiming to evaluate moisture exchange capacity . It was utilized the experimental design split plot with five replications (blocks), six principal treatments (parcels) and nine secondary treatments (sub parcels, weighing of hour in hour, of 08h00min. until 16h00min.). In the period of eight hours of experimentation mulberry leaves moisturedevreased in all the ambients evaluated. However, for cover leaves with wet cloth, for all periods, the water purport (74,63%) was maintained close to the original value (08h00min.- 76,07%), demonstrating the efficiency technique in the conservation of the humidity of leaves for feeding silkworm.