Study of seed-germination and etiolation of seedlings in Emilia-sonchifolia (l) dc and Emilia-fosgerbii nicolson (Asteraceae)

The germination of seeds of Emilia sonchifolia (2n) and E. fosgerbii (4n) was analysed and our results indicate that the former present light insensitive seeds and the latter germinated only under continuous white light. The germination of seeds under shade light presented no differences between both species, However under continuous white light, which maintain 70% of Pfr, the velocity of germination was greater in Emilia sonchifolia when compared to Emilia fosbergii, indicating that the threshold of 4n seeds were greater than 2n seeds. The analysis of the fluence response curves for etiolating process indicated that the process presented no differences between both species. The same was observed for the effect of the shade light in the etiolating process.

Desiccation tolerance of four seedlots from Euterpe edulis Mart.

In order to identify the desiccation tolerance of E. edulis seeds, four fruitlots were collected from the Campinas Agronomic Institute Palmae collection, located at Ubatuba, state of Sao Paulo, Brazil, and taken in moisture-proof containers to the College of Agriculture in Botucatu, state of Sao Paulo. There, the fruits were shelled and the seeds were dried, either under silica gel conditions or in a drying chamber. The effects of dehydration were evaluated by measurements of the germination, embryo protrusion, germinative button, plumule, speed of emergence, seedling length, seed dry matter and water content. Differences among seedlots and between harvest season for most of the traits were observed. Euterpe edulis seedlots seem to tolerate water content reductions until 39% without significant reduction in germination and vigor. Below this value, most of the evaluated traits were negatively affected. Total loss of germination was observed whenever seed water content was reduced to values below 21%.

Storage in cerrado soil and germination of Psychotria vellosiana (Rubiaceae) seeds

The regeneration of plant communities from seed depends, to a large extent, on the capacity of the seed remaining viable in the soil. The viability and germination of artificially buried Psychotria vellosiana seeds in cerrado soil were studied, with the purpose of discovering some physio-ecological aspects of dispersed seeds and evaluating their potential to constitute a soil seed bank. Seed samples were placed in nylon envelopes and buried in the soil of a Cerrado reserve at two different depths and sites. Buried seeds were retrieved periodically and tested for germination along with dry-stored seeds. In general, there was a reduction in seed germination with storage time, both in soil and dry stored conditions, and in some assays exhumed seeds germinated faster than dry stored ones. In general the soil storage favoured seed viability of ungerminated seeds as compared to dry stored ones, with the seeds remaining partially viable after 10 months of storage. The lack of germination of viable seeds suggests that seeds showed true dormancy and/or required an extended time to germinate. It was observed that some seeds had germinated while buried and such in situ germination tended to increase with rainfall. The water availability in the soil might be a limiting factor for successful germination of P. vellosiana in the field, and the seeds may constitute a persistent soil seed bank in the cerrado as dispersed seeds remain viable in the soil until the following period of seed dispersal.

Effects of gender on stroke rates, critical speed and velocity of a 30-min swim in young swimmers

Our objective was to analyze the effect of gender on the relationship between stroke rates corresponding to critical speed (SRCS) and maximal speed of 30 min (SRS30) in young swimmers. Twenty two males (GM1) (Age = 15.4 ± 2.1 yr., Body mass = 63.7 ± 12.9 kg, Stature = 1.73 ± 0.09 m) and fourteen female (GF) swimmers (Age = 15.1 ± 1.6 yr., Body mass = 58.3 ± 8.8 kg, Stature = 1.65 ± 0.06 m) were studied. A subset of males (GM2) was matched to the GF by their velocity for a 30 min swim (S30). The critical speed (CS) was determined through the slope of the linear regression line between the distances (200 and 400 m) and participant's respective times. CS was significantly higher than S30 in males (GM1 - 1.25 and 1.16 and GM2 - 1.21 and 1.12 m·s-1) and females (GF - 1.15 and 1.11 m·s-1). There was no significant difference between SRCS and SRS30 in males (GM1 - 34.16 and 32.32 and GM2 - 34.67 and 32.46 cycle·s-1, respectively) and females (GF - 34.18 and 33.67 cycle·s-1-1, respectively). There was a significant correlation between CS and S30 (GM1 - r = 0.89, GF - r = 0.94 and GM2 - r = 0.90) and between SRCS and SRS30 (GM1 - r = 0.89, GF - r = 0.80 and GM2 - r = 0.88). Thus, the relationship between SRCS and SRS30 is not influenced by gender, in swimmers with similar and different aerobic capacity levels. ©Journal of Sports Science and Medicine (2007).

Estimation of electrical machine speed using sensorless technology and neural networks

The use of sensorless technologies is an increasing tendency on industrial drivers for electrical machines. The estimation of electrical and mechanical parameters involved with the electrical machine control is used very frequently in order to avoid measurement of all variables related to this process. The cost reduction may also be considered in industrial drivers, besides the increasing robustness of the system, as an advantage of the use of sensorless technologies. This work proposes the use of a recurrent artificial neural network to estimate the speed of induction motor for sensorless control schemes using one single current sensor. Simulation and experimental results are presented to validate the proposed approach. ©2008 IEEE.

Allelopathic effects of aqueous extract of Brassica napus on germination of seeds of Phaseolus vulgaris

Secondary compounds produced by plants are considered an alternative method of weed suppression but can cause negative effects on crops in succession, especially in a no-tillage system, due to the degradation of crop residues with allelopathic potential. The objective of this work was to analyze the influence of foliar aqueous extracts of Brassica napus on the germination and initial development of seedlings of Phaseolus vulgaris. The extract was prepared as a stock 10 % weight/volume solution, and diluted into treatments of relative concentrations of 100 % (i.e. 10 % w/v stock), 75 %, 50 %, 25 % and 0 % (untreated control consisting of distilled water), in a completely randomized design. The seeds of Phaseolus vulgaris were moistened with the differing concentrated extracts and kept in a germination chamber at 25 °C, with a photoperiod of 12 h for nine days. The variables evaluated were: percentage germinating, first count of germination and germination velocity index, as well the root and hypocotyl length, and fresh and dry mass of the seedlings. The aqueous leaf extracts of Brassica napus did not influence the germination of Phaseolus vulgaris seeds, but did induce the growth of abnormal seedlings by inhibition of secondary roots and reduced prominence of the primary root.

Propagation of Camptosema grandiflorum native to Brazil by seeds and cuttings

Camptosema grandiflorum Benth., belonging to Fabaceae, is a voluble climber plant native to Brazil. Plants bloom in autumn-winter, producing long and hanging inflorescences with showy red flowers, which are much visited by hummingbirds. The leaves are also attractive, composed by three leaflets. It can be propagated by seeds or cuttings, but both seed germination and cutting rooting percentages are very low. Thus, the objective of this work was to study the effect of different temperatures on seed germination and of different indolebutyric acid (IBA) concentrations on the rooting of cuttings of C. grandiflorum. The experiment was set up at the São Paulo State University, located in Jaboticabal, São Paulo State, Brazil. The germination study was conducted in an entirely randomized design with six different temperatures (constant at 20, 25, 30 and 35°C; and alternated at 20-30 and 25-35°C, with a photoperiod of 12 hours) and four replications of 25 seeds each, placed in plastic boxes with vermiculite. The percentage of germination and the speed germination index (SGI) were evaluated. An entirely randomized block design was adopted for the cutting rooting evaluation, with four IBA concentrations (0; 1,000; 2,000; and 3,000 mg kg-1) and five replications of ten cuttings each, comprising 200 cuttings. After 30 days from the beginning of the rooting experiment, data referring to rooting percentage, number and length of roots and dry weight of roots were collected. For the seed germination experiment, fastest germination and highest germination percentage (87%) were obtained when seeds were maintained under the constant temperature of 30°C. For the cutting experiment, the concentrations of 2,000 and 3,000 mg kg-1 of IBA promoted the highest rooting percentages (98.5 and 94.1%, respectively) and number of roots. There were no statistical differences among the IBA concentrations for length of roots and dry weight of roots.

Ecophysiology of seed germination in Digitaria insularis ((L.) Fedde)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Allelopathic potential and survey of natural seed bank under the canopy of Copaifera Langsdorffii Desf

The aim of this study was to evaluate the allelopathic effect of soil samples collected under the canopies of three specimens of Copaifera langsdorffii the germination of Lactuca sativa and survey the natural seed bank according to seasonality. To test the allelopathic effect was carried experiments of pre and post-emergence with seeds of L. sativa and to quantify the stock of seeds, soil samples were collected from three specimens at three distances from the stem (1, 2 and 3 m) and at three depths (0-5, 5-10 and 10-15cm) in the region of savanna in the dry and wet seasons. The samples tested in bioassay of pre-emergence no significant influence on germinability, mean germination time and mean germination speed, but showed a difference in the synchronism of germination, these data were independent of sampling station. In test for post-emergence was observed statistical difference in the parameters evaluate (length of primary roots and hypocotyls) in both seasons. To quantify the natural seed bank were macroscopic analysis of each soil sample with the help of stereoscopic microscope. The analysis of the natural seed bank showed a larger number of seeds in the 0-5cm and in distance of 2m for both seasons. The results suggest the presence of allelochemical substances in soil samples collected under the canopy of Copaifera langsdorffii.

Evaluation of the best-designed graded exercise test to assess peak treadmill speed

This study examined the influence of different speed increments during treadmill exercise tests on peak treadmill speed (Vpeak) and its relationship with a 1-h treadmill running performance. 18 male recreational and amateur runners (10-km running pace: 10–15 km·h−1) performed, in an alternate order, 3 continuous incremental exercise tests with different speed increments (0.5, 1.0 and 2.0 km·h−1) on a motorized treadmill to determine Vpeak. Thereafter they undertook a 1-h time trial on a treadmill. Vpeak was determined as either (a) the highest speed that could be maintained for a complete minute (Vpeak-60 s), (b) the speed of the last complete stage (Vpeak-C), or (c) the speed of the last complete stage added to the product of the speed increment and the completed fraction of the incomplete stage (Vpeak-P). The Vpeak values were highly influenced by the different speed-incremented rates and the Vpeak-P determined during the protocol comprising speed increments of 1 km·h−1 presented the highest correlation with 1-h time trial performance (r=0.89). The results suggest that a protocol with speed increments comprising 1 km·h−1 and with a 3-min stage duration should be used as standard for the determination of Vpeak to assess aerobic fitness and predict endurance performance in recreational runners. Furthermore, the Vpeak-P should be used for the determination of Vpeak.

Controle em pós-emergência e características germinativas de agriãozinho

O agriãozinho é uma planta daninha de grande importância em pastagens do Brasil e apresenta destacada agressividade, sendo seu controle, portanto, desejável para o sucesso da produção forrageira. O objetivo deste trabalho foi avaliar o controle químico de Synedrellopsis grisebachii na fase reprodutiva e as suas consequências sobre as características germinativas dos aquênios da planta daninha. Os tratamentos constaram da aplicação dos herbicidas glyphosate (100, 200, 900 e 1.800 g ha-1), paraquat (34, 68, 300 e 600 g ha-1) e triclopyr (75, 150, 667 e 1.334 g ha-1), além da testemunha sem aplicação. Foram coletados aquênios aos 15 dias após a aplicação, sendo estes submetidos ao teste de germinação, determinando-se a porcentagem e o índice de velocidade de germinação. Após 29 dias em germinação, verificou-se a viabilidade dos aquênios não germinados, através do teste de tetrazólio. A eficácia dos herbicidas foi avaliada por meio de notas visuais de controle aos 7, 14, 21 e 28 DAA. Conclui-se que para o controle total de S. grisebachii, em estádio reprodutivo, é necessária a aplicação de 1.334 g ha-1 de triclopyr. Nesse estádio, a planta apresentou grande tolerância ao glyphosate e também ao paraquat. Quanto às características germinativas da progênie, o herbicida triclopyr nas doses de 150 e 667 g ha-1 promoveu redução na velocidade de germinação e na viabilidade, enquanto o glyphosate e paraquat não proporcionaram efeito.

Aplicação de zinco via sementes e seu efeito na germinação, nutrição e desenvolvimento inicial do sorgo

O objetivo deste trabalho foi avaliar o efeito da aplicação de zinco em sementes de sorgo (Sorghum bicolor (L.) Moench) na nutrição, na germinação e no desenvolvimento inicial das plantas, em casa de vegetação. Utilizou-se delineamento inteiramente casualizado dos tratamentos, em esquema fatorial 2x5, duas cultivares de sorgo (BR 304 e BR 310) e cinco doses de Zn (0, 3,57, 7,14, 14,28 e 28,56 g de Zn por kg de sementes), com três repetições. Foram avaliadas as variáveis de germinação (tempo médio, média diária e a velocidade), e 28 dias depois da semeadura, determinou-se a massa da matéria seca e os teores de Zn na parte aérea e nas raízes. A aplicação de Zn nas sementes de sorgo não afetou o acúmulo de matéria seca da parte aérea, entretanto, diminuiu a germinação e o acúmulo de matéria seca das raízes e da planta inteira. O Zn aplicado nas sementes de sorgo acumulou-se principalmente nas raízes. A cultivar BR 304 apresentou maior absorção de Zn e acúmulo de matéria seca, em relação à cultivar BR 310.

Operational parameters of soybean seeding in santa fe system

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção, transmissão e efeito de Xanthomonas campestris pv. campestris na qualidade fisiológica de sementes de brócolis

A detecção, a transmissão e o efeito de Xanthomonas campestris pv. campestris (Xcc) na qualidade fisiológica de sementes de brócolis (Brassica oleracea var. italica) foram avaliados, a partir de sementes obtidas de plantas (Baron, Flórida, Hana Midori Sakata, Precoce Piracicaba de Verão, Ramoso Santana e Sabre) inoculadas com a bactéria, em condições de campo. Para a detecção do patógeno nas sementes foram utilizados os meios de cultura semi-seletivos: SX ágar, NSCAA e BSCAA; a taxa de transmissão da bactéria pelas sementes às plântulas foi avaliada usando semeadura em areia e meio de cultura contido em tubo de ensaio. Para a avaliação da qualidade fisiológica de sementes foram realizados o teste padrão de germinação e os testes de vigor: envelhecimento acelerado, índice de velocidade de emergência, crescimento de plântulas e massa seca. de acordo com os resultados, o meio de cultura semi-seletivo NSCAA foi mais eficaz para detectar Xcc em sementes de brócolis; não houve diferença significativa entre os genótipos na taxa de transmissão da bactéria pelas sementes e Xcc não afetou a germinação e o vigor das sementes.

Exercise intensity, but not electrolyte reposition, alters plasmatic cortisol and glucose levels of horses submitted to 30 and 60km distance endurance rides

O presente estudo teve como objetivo comparar os níveis plasmáticos de glicose e cortisol de um grupo de eqüinos atletas submetidos ao esforço de longa duração (enduro eqüestre) em distâncias de 30 e 60km, com velocidades médias de 10 e 15km h-1, respectivamente. Os animais foram aleatoriamente separados em dois grupos, sendo que a um deles foi administrada, por via oral, uma pasta eletrolítica hipertônica antes, durante e após as referidas provas. O outro grupo foi usado como controle. Os resultados das concentrações de cortisol revelaram um aumento significativo, no primeiro momento, em que a intensidade do esforço era maior (15km/h), ou com a continuação deste (30km de distância), no segundo momento, para todos os grupos. Os resultados referentes à concentração de glicose permaneceram constantes durante a prova de 30km; entretanto, nos primeiros 20km da prova de 60km, tais resultados revelaram um aumento significativo no grupo controle, retornando a valores basais no momento subseqüente. A reposição eletrolítica tem a sua importância na manutenção do equilíbrio hidroeletrolítico e ácido básico e, no presente estudo, pode ter contribuído para a manutenção da concentração média de glicose plasmática.