Lipoperoxidação, perda de viabilidade celular e diminuição da liberação de IL-8 de neutrófilos humanos na presença de corpos cetônicos

Type-1 diabetes patients suffer from frequent episodes of acidosis caused by an increased fatty acid metabolism and consequently increased plasma level of acetoacetate (AcAc) and β-hydroxybutyrate (β-HOB). This article describes a study of the effects of pathological concentrations of AcAc and β-HOB on lipoperoxidation, cell viability and the release of the CXCL8 (IL-8) cytokine by activated neutrophils. Neutrophils from healthy donors were isolated by density gradient (Histopaque® 1077/1119) and incubated with the ketone bodies. Lipoperoxidation was determined as thiobarbituric acid reactive substances (TBARS). The cell viability was evaluated by the release of intracellular lactate dehydrogenase. The release of CXCL8 was measured by ELISA in a 24-h culture of opsonized zymosan-stimulated neutrophils. AcAc, but not β-HOB, provoked a dose-dependent increase in the neutrophil membrane lipoperoxidation (p<0.05; r =0.9915). In the cytotoxicity assay, a dose-dependent release of LDH was observed when the neutrophils were incubated with AcAc in concentrations up to 40 mM (p<0.05). β-HOB was devoid of effect. The release of CXCL8 was inhibited by AcAc and β-HOB in a dose-dependent manner. In conclusion, these results suggest that the accumulation of ketone bodies in diabetic patients could be involved in their usually increased susceptibility to infection.

Organização celular dos testículos em Hylidae e leptodactylidae, no Pantanal (Estado do Mato Grosso do Sul, Brasil)

The objective of this work was to make a comparative analysis of germ cell organization at different stages of cellular differentiation in adult males of Dendropsophus nanus (Boulenger, 1889), Pseudis limellum (Cope, 1862), P. paradoxa (Linnaeus, 1758), and Scinax acuminatus (Cope, 1862), belonging to the family Hylidae; and Leptodactylus chaquensis (Cei, 1950) and L. podicipinus (Cope, 1862), belonging to the family Leptodactylidae, collected in the Pantanal and in Serra da Bodoquena, Mato Grosso do Sul State, Brazil. The testes were removed and fixed, dehydrated in a graded series of ethanol and embedded in methacrylate glycol resin (Historesin Leica®). The sections were stained by 1% toluidine blue and observed under light microscope. It was detected that all individual of the Hylidae family show, throughout the year, the presence of all germ cell types of spermatogenesis. However, all Leptodactylidae family individuals only show the presence of all germ cell types during the rainy season. The variations of characteristics in seminiferous epithelium organization, as well as the evident difference in the amount of spermatozoa inside the tubules, are evidence that the anurans in this work show different forms of spermatogenesis development throughout the year: the cycle is continuous for the Hylidae family, and discontinuous with explosive release of spermatozoa for the Leptodactylidae family.

Efeito da temperatura no desenvolvimento e sobrevivência da cochonilha pseudococcus longispinus (Targioni Tozzetti, 1867) (hemiptera: Pseudococcidae) em cafeeiro

The longtailed mealybug Pseudococcus longispinus (Targioni Tozzetti, 1867) (Hemiptera: Pseudococcidae) has been reported attacking coffee crops causing fruit fall in the State of Minas Gerais, Brazil. The knowledge of the population dynamic of this pest is then necessary to implement control measures. Its development was studied at temperatures of 15, 20, 25, 30 and 35°C, determining the thermal requirements. The insects were confined inside a Petri dish containing a foliar disc of 4 cm diameter of Coffea arabica L., cultivar 'Acaiá Cerrado'. The temperature affected the P. longispinus development and survival. Few insects survived at temperatures of 15 and 30°C, and 100% of mortality was obtained at 35°C. The duration of the nymphal stage was reduced when the temperature was increased from 20 to 25°C, with a survival rate of 80% at both temperatures. The thermal parameters varied according to the development stage of the mealybug and the base temperature was fixed at 8.0 °C for the nymphal stage of females, a thermal constant of 422.1 day degrees and number of generations increased with rising temperature. The optimal temperature for the insect development was 25°C.

Estado nutricional e níveis plasmáticos de trauma e seus precursores em pacientes portadores de neoplasias malignas de esôfago

Pós-graduação em Bases Gerais da Cirurgia - FMB

Atividade mutagênica e ativadora da resposta imune celular induzidas por Byrsonima crassa Niedenzu e Byrsonima intermedia A.Juss. (Malpighiaceae)

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Efeito de dietas e altura da coluna d’água na sobrevivência de larvas de Betta splendens e o aporte de nitrogênio e fósforo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Utilização do 'beta' - glucano sobre o desempemho produtivo, indicadores de estresse, perfil hematológico e sobrevivência do pacu (Piaractus mesopotamicus)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da densidade de estocagem na sobrevivência e crescimento do pampo (Trachinotus carolinus) em tanques-rede marinhos

Pós-graduação em Aquicultura - FCAV

Vitamina E em dietas para reprodutoras de tilápia-do-nilo (Oreochromis niloticus)

Pós-graduação em Aquicultura - FCAV

Treinamento alimentar de pintado Pseudoplatystoma coruscans (Agassiz, 1829): sobrevivência, crescimento e aspectos econômicos

Pós-graduação em Aquicultura - FCAV

Alocação de canais em sistemas de comunicação celular empregando algoritmo genético distribuído

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Monitoramento de dados via internet baseado em telefonia celular

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação dos efeitos citotóxicos, genotóxicos e mutagênicos de efluentes de refinaria de petróleo, por meio dos sistemas testes de Allium cepa e Oreochromis niloticus

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Avaliação dos potenciais citotóxico, genotóxico e mutagênico das águas de um ambiente lêntico, por meio dos sistemas-teste de Allium cepa e Oreochromis niloticus

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito α-tomatina na proliferação celular, apoptose e expressão de RNAm dos genes APC, Ciclina A2, Catenina, CASP9, BAK, BAX e BCL-XL em células HT29

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)