Análise filogenética e revisão taxonômica de Hasemania Ellis, 1911 (Characiformes, Characidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A espermiogênese e a ultraestrutura dos espermatozóides de representantes de alguns gêneros incertae sedis em characidae, anteriormente alocados em tetragonopterinae(Teleostei: Characiformes) com ênfase em Moenkhausia, e suas aplicações filogenéticas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Enteroparasitoses em indivíduos HIV/aids: aspectos epidemiológicos, nutricionais e imunológicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Epidemiologia das parasitoses intestinais e caracterização genotípica de isolados de Giardia duodenalis de escolares do município de Pratânia, estado de São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Infecção experimental de Mazana gouazoubira (Ficher, 1814) (Cervidae: Odocoileinae) com Haemonchus contortus (Rudolphi, 1803) (Nematoda: Trichostrongyloidea)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO

A pesquisa de infecções por Giardia e a caracterização genotípica deste protozoário foi realizada em primatas não humanos (PNH) mantidos em Zoológico a fim de avaliar o seu potencial zoonótico. As amostras dos animais consistiram de fezes colhidas do piso de 22 baias onde eram mantidos 47 primatas de 18 diferentes espécies. Exames coproparasitológicos foram realizados pelos métodos de concentração por sedimentação e centrífugo-flutuação e revelaram a presença dos seguintes parasitas e suas respectivas frequências: Giardia (18%); Entamoeba spp. (18%); Endolimax nana (4.5%); Iodamoeba spp. (4.5%); oxiurídeos (4.5%) e estrongilídeos (4.5%). O DNA extraído de todas as amostras fecais foi submetido à técnica de PCR para a amplificação dos genes gdh e tpi de Giardia, porém, só foram obtidos amplicons das quatro amostras positivas provenientes de Ateles belzebuth, Alouatta caraya, Alouatta fusca and Alouatta seniculus. O seqüenciamento dos fragmentos amplificados foi possível apenas para as amostras oriundas de Ateles belzebuth (BA1), Alouatta fusca (BA2) e Alouatta caraya (BA3), cuja análise fenética de ambos os genes revelou pertencerem ao genótipo A. As análises das sequências de tpi revelaram que todas as amostras pertencem ao subgenótipo AII. No que se refere ao gene gdh as análises revelaram uma amostra pertencente ao subgenótipo AII (BA3) e duas ao subgenótipo A1 (BA1 e BA2). Considerando o potencial zoonótico do genótipo A e o fato de que os animais não apresentavam sintomas de infecção, os dados do presente trabalho salientam a importância de se realizar, periodicamente, exames coproparasitológicos dos animais de zoológico, para implementação de medidas preventivas para resguardar a saúde dos animais em cativeiro, a de seus tratadores e dos visitantes de parques zoológicos.

The role of chromosome variation in the speciation of the red brocket deer complex: the study of reproductive isolation in females

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Levantamento da mastofauna terrestre de médio e grande porte em remanescente de floresta neotropical – Itatinga, SP

The Brazil´s Atlantic coastal forest biome is considered one of the richest and most exuberant biodiversity of the whole planet. Still, other data shows the reality of this Brazilian forest: more than 93% of its original formation has been already devastated, being the human actions the greatest threat to the perpetuation of the balance and diversity of this ecosystem. This destruction affects all the fauna and flora, with even greater impact on medium and large mammals, due to its high demand on the availability of resources, living area and sensitivity to disturbances. However, today it is known the vital importance of these mammals in the preservation and maintenance of biological systems. Thus, the purpose of this study was to survey the species richness of medium and large mammals in a remnant neotropical forest located in Itatinga, SP,Brazil, using the indirect method of recording (traces on sand-plots, marking territorial, faeces, carcasses, scratches on tree trunks and vocalizations) and camera trapping. Data were collected during ten (10) consecutive months in the year 2008, from February to November. Within twenty-five plots of sand installed and monitored during the ten months were found fifteen (15) species of terrestrial mammals of medium and large, five (5) orders and twelve (12) families. Among them, we highlight the presence of threated species like the puma (Puma concolor), the deer (Mazama gouazoubira) and the giant anteaters (Myrmecophaga tridactyla). The chosen method showed up effective in raising terrestrial mammalian species of medium and large neotropical forest area, having a good applicability and advantages over other existing methods. The study area presents a huge diversity of species and, therefore, this work it is recommended to be only the beginning of new researches in the area in order to enrich... (Complete abstract click electronic access below)

Levantamento da mastofauna e identificação das influências antrópicas em dois fragmentos de Mata Atlântica transição com Cerrado de Rubião Junior – Botucatu - SP

Atlantic Forest and Cerrado are between the biological richest areas in the world, for that are considered top conservation priorities. Those ecosystems are found at Botucatu region, where it´s extremely fragmented by routes, agricultural areas and urban centres. This fragmentation causes restrictions on animals populations, in particular to medium and large mammals, that needs larger areas to live and also are often hunted. Even isolated and suffering anthropic influences the fragments are fundamental for local biodiversity conservation. We realized a mammal survey on two forest fragments of Atlantic forest transition to Cerrado of Rubião Júnior – Botucatu – SP – Brazil: Santo Antônio Church and Parque das Cascatas forests. The Santo Antônio church munt is considered a turistic point and its forest receive many visitants, Parque das Cascatas forest is inside of a residential condominium. Both areas are classify as semidecidual stational forest and are disconected by Domingos Sartori route, that connect Botucatu centre to Rubião Júnior district. Around both fragments can be found residences, plantations and pastures. The utilized method included: recognition of tracks on the forests, interviews with next residents and sand plots mounting. The interviews indicated 29 mammals species, and 19 was validated for animal traces, overruns or visualization. Other 3 species found were not mentioned on interviews. Totally, was found 22 small and medium mammals presence evidences on both forest fragments. Parque das Cascatas forest showed more diversity (19) then Santo Antônio Church forest (11), and species like Eira barbara, Procyon cancrivorus, Mazama americana, Shiggurus villosus, Dasypus novemcinctus, Didelphis albiventris, Lutreolina crassicaudata and Lepus europaeus looked for other areas once that was verifyed overruns of individuals... (Complete abstract click electronic access below)

Parasitos e comensais intestinais em pacientes neoplásicos submetidos à quimioterapia

The objective of this study was to verify the prevalence of intestinal parasites and/or commensals in the neoplastic patients undergoing chemotherapy. Stool samples were analyzed by the method of Lutz (1919) and Rugai (1954), in triplicate. This work was composed of three groups, the first one (GI) formed by neoplastic patients that are not undergoing chemotherapy, the second (GII) comprised patients who were undergoing chemotherapy, and the third group (GIII) consisting of patients who completed chemotherapy. A total of 30 patients (GI-5, GII-18 and GIII-7) were screened at the Assis Regional Hospital of the Unified Health System of Assis, São Paulo.Additional information on antiparasitic treatment and tumor type were obtained by questionnaire. The positivity was 66.7% (20 cases) for intestinal parasites and/or commensals. The helminths were Ascaris lumbricoides (36.7%), Hookworms (20%) and Hymenolepis diminuta (3.3%). Among the highlights are protozoan Giardia lamblia (46.7%), Entamoeba coli (6.7%), E. histolytica /dispar (3.3%), Endolimax nana (3.3%) and Iodameba butschlii (3.3%). The high frequency of intestinal parasites and/or commensals in the neoplastic patients can be attributed to poor personal hygiene and lack of immunity to reinfection and poor knowledge of the prophylaxis of infection by protozoa and helminths. The results indicate the necessity of adopting a new criterion for neoplastic patients undergoing chemotherapy, primarily performing parasitological diagnosis, treatment and monitoring of cure of intestinal parasitic infections in this risk group.

Diagnóstico parasitológico de horticultures no monitoramento da contaminação parasitária em ambientes rurais

The objective of this study was to detect possible carriers of parasites and/or commensals between the horticulturists of Fair of the Producer from Upper Paranaiba, Minas Gerais. A total of 30 horticulturists were instructed to collect three stool samples on alternate days during the months of August and September 2007. Horticulturists were positive 40% for one or more parasite and / or dinner, and found: Giardia lamblia (3.5%), Entamoeba histolytica / E. dispar (7%), Entamoeba coli (13%), Endolimax nana (13%) and Ascaris lumbricoides (3.5%). Stool of gardeners is of importance in monitoring parasite sanitary conditions of vegetables sold in urban environments.

Synchronization enhancement via an oscillatory bath in a network of self-excited cells

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nanoparticles increase the efficacy of cancer chemopreventive agents in cells exposed to cigarette smoke condensate

Lung cancer is a leading cause of death in developed countries. Although smoking cessation is a primary strategy for preventing lung cancer, former smokers remain at high risk of cancer. Accordingly, there is a need to increase the efficacy of lung cancer prevention. Poor bioavailability is the main factor limiting the efficacy of chemopreventive agents. The aim of this study was to increase the efficacy of cancer chemopreventive agents by using lipid nanoparticles (NPs) as a carrier. This study evaluated the ability of lipid NPs to modify the pharmacodynamics of chemopreventive agents including N-acetyl-L-cysteine, phenethyl isothiocyanate and resveratrol (RES). The chemopreventive efficacy of these drugs was determined by evaluating their abilities to counteract cytotoxic damage (DNA fragmentation) induced by cigarette smoke condensate (CSC) and to activate protective apoptosis (annexin-V cytofluorimetric staining) in bronchial epithelial cells both in vitro and in ex vivo experiment in mice. NPs decreased the toxicity of RES and increased its ability to counteract CSC cytotoxicity. NPs significantly increased the ability of phenethyl isothiocyanate to attenuate CSC-induced DNA fragmentation at the highest tested dose. In contrast, this potentiating effect was observed at all tested doses of RES, NPs dramatically increasing RES-induced apoptosis in CSC-treated cells. These results provide evidence that NPs are highly effective at increasing the efficacy of lipophilic drugs (RES) but are not effective towards hydrophilic agents (N-acetyl-L-cysteine), which already possess remarkable bioavailability. Intermediate effects were observed for phenethyl isothiocyanate. These findings are relevant to the identification of cancer chemopreventive agents that would benefit from lipid NP delivery.

Anatomical description of arterial segment of spleen of deer

The anatomosurgical segmentation of the arteries of the spleen was studied in 31 deer of the species Mazama gouazoubira and Blastocerus dichotomus by means of vascular injection with latex and vinyl acetate and radiographic examination. The arteria lienalis penetrated through the hilus lienis in 87% of the cases, whereas an extrahilar artery was present in the other cases. An extraparenchymal division of the lineal artery into two, three or four segmental arteries was observed in 74% of the cases. Anastomoses between intraparenchymal arterial branches were rare and of a reduced calibre.