Cyclodextrin Production by Bacillus lehensis Isolated from Cassava Starch: Characterisation of a Novel Enzyme

The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using beta-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cydodextrin glycosyltransferase were 55 degrees C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h.

Associação de variedades de goiaba, bioinseticidas e o parasitóide Diachasmimorpha longicaudata no controle de Anastrepha fraterculus

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Avaliação de genótipos de maracujazeiro e viabilidade econômica da cv. Roxinho do Kênia para exportação

Pós-graduação em Agronomia (Horticultura) - FCA

Use of EP(Peroxidase) allele in soybean varietal characterization

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento inicial e absorção de zinco pelo milho em função do modo de aplicação e fontes do nutriente

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morfometria, umidade do solo e risco de erosão laminar da microbacia do córrego pimenta da fazenda São Manuel

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Response of pullets to digestible lysine intake

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência do uso de malhas de sombreamento azul, vermelha e preta no desenvolvimento inicial do pepino Taiko

Cucumber (Cucumis sativus L.) is a plant of the Cucurbitaceae family of great commercial importance and their culture is widespread throughout the country. The production of vegetables, high-quality care begins with training ranging from seedlings and shading techniques have an important role in creating environments more conducive to the production of seedlings and many researchers and producers of seedlings have opted for the use of color shading screens, making this way, the wavelength and the amount of incident energy are changed in favor of the plants. Work will be performed at the Department of Forest Sciences, Faculty of Agricultural Sciences, Universidade Estadual Paulista (UNESP), Botucatu campus. The statistical design will be in three blocks, each containing 100 plots of each treatment (seedlings covered with blue screen, red and black). Evaluations of dry mass and height will be made at 7, 14, 21 and 28 days after emergence, and 5 will be selected seedlings of each treatment. At 14 and 28 DAE was measured, and SPAD chlorophyll content at 30 DAE, was evaluated by measuring gas exchange in the second pair of leaves, fully expanded for each plant, which was put in a chamber controlled with infrared gas analyzer (IRGA LI 6400, Licor) to perform measurements of photosynthesis. Treatment with black shading showed statistically better results for total dry matter, plant height, SPAD chlorophyll content and net assimilation of CO2, showing a better quality seedlings and its use is recommended in the cultivation of cucumber Taiko

Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros

Pós-graduação em Medicina Veterinária - FCAV

Resistência de cultivares de milho convencional e transgênico à Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae)

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Balanço hídrico, crescimento e produtividade de genótipos RB de cana-de-açúcar em cultivo de sequeiro na região de Rio Largo-AL

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Utilização de resíduos de tratamento de esgoto como suprimento hídrico e nutricional na cultura do girassol

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Clones de Eucalyptus sp. submetidos a estresse hídrico em dois tipos de solo

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Aplicação de regulador vegetal e poda nas relações fisiológicas na produção do tomateiro

Pós-graduação em Agronomia (Horticultura) - FCA