Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones

Bacterial artificial chromosomes (BAC) have been widely used for fluorescence in situ hybridization (FISH) mapping of chromosome landmarks in different organisms, including a few in teleosts. In this study, we used BAC-FISH to consolidate the previous genetic and cytogenetic maps of the turbot (Scophthalmus maximus), a commercially important pleuronectiform. The maps consisted of 24 linkage groups (LGs) but only 22 chromosomes. All turbot LGs were assigned to specific chromosomes using BAC probes obtained from a turbot 5x genomic BAC library. It consisted of 46,080 clones with inserts of at least 100 kb and < 5 % empty vectors. These BAC probes contained gene-derived or anonymous markers, most of them linked to quantitative trait loci (QTL) related to productive traits. BAC clones were mapped by FISH to unique marker-specific chromosomal positions, which showed a notable concordance with previous genetic mapping data. The two metacentric pairs were cytogenetically assigned to LG2 and LG16, and the nucleolar organizer region (NOR)-bearing pair was assigned to LG15. Double-color FISH assays enabled the consolidation of the turbot genetic map into 22 linkage groups by merging LG8 with LG18 and LG21 with LG24. In this work, a first-generation probe panel of BAC clones anchored to the turbot linkage and cytogenetical map was developed. It is a useful tool for chromosome traceability in turbot, but also relevant in the context of pleuronectiform karyotypes, which often show small hardly identifiable chromosomes. This panel will also be valuable for further integrative genomics of turbot within Pleuronectiformes and teleosts, especially for fine QTL mapping for aquaculture traits, comparative genomics, and whole-genome assembly.

Amazon forest biomass density maps: tackling the uncertainty in carbon emission estimates

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Relaxation to Fixed Points in the Logistic and Cubic Maps: Analytical and Numerical Investigation

Convergence to a period one fixed point is investigated for both logistic and cubic maps. For the logistic map the relaxation to the fixed point is considered near a transcritical bifurcation while for the cubic map it is near a pitchfork bifurcation. We confirmed that the convergence to the fixed point in both logistic and cubic maps for a region close to the fixed point goes exponentially fast to the fixed point and with a relaxation time described by a power law of exponent -1. At the bifurcation point, the exponent is not universal and depends on the type of the bifurcation as well as on the nonlinearity of the map.

COINCIDENCES OF FIBREWISE MAPS BETWEEN SPHERE BUNDLES OVER THE CIRCLE

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biogeographic Distribution Patterns and Their Correlates in the Diverse Frog Fauna of the Atlantic Forest Hotspot

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determination of lead in eye shadow and blush by high-resolution continuum source graphite furnace atomic absorption spectrometry employing directSolid sampling

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Structural damage detection in an aeronautical panel using analysis of variance

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Implantes curtos do tipo cone-Morse: proporção coroa-implante

The purpose of this study was to analyze the biomechanical interactions in bone tissue between short implants and implant-supported crowns with different heights. Two models were made using the programs InVesalius 3.0, Rhinoceros 4.0 and Solidworks 2010. The models were established from a bone block with the short implant (3.75 x 8.5 mm) with geometry Morse taper connection (MT). The height of the crown (cemented) was set at 10.0 mm and 15.00 mm. The models were processed by programs and 10 NEiNastran Femap 10.0. The force applied was 200N (vertical) and 100N (oblique). The results were plotted on maps Voltage Maximum Principal. Statistical analysis was performed using ANOVA. The results showed that the increase in crown height, increased stress concentration in the crown of 15 mm under oblique loading (p <0.001), the oblique loading has significantly expanded the area of stress concentration (p <0.001). Conclusion:the increase of the crown increased the stress concentration, being statistically significant for short implants Morse taper. The mesial and distal region had the highest concentration of stresses under oblique loading. The oblique loading was more harmful when compared with axial loading, being statistically significant.

Precision of distances and ordering of microsatellite markers in consensus linkage maps of chromosomes 1, 3 and 4 from two reciprocal chicken populations using bootstrap sampling

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)