Idade masculina: Qualidade do sêmen e fertilidade: Idade masculina X qualidade seminal

The man's age seems to affect reproductive function but to a lower degree compared with the woman's age. In fact, the effect of paternal age on semen quality has been discussed, but the results are contradictory. Considering the differences in the results of studies, this review aimed to analyze the literature regarding the effect of paternal age on semen parameters (volume, concentration, motility and morphology) in sperm DnA damage in assisted reproduction results and genetic diseases.

Effect of Removing Seminal Plasma Using a Sperm Filter on the Viability of Refrigerated Stallion Semen

Cooling of equine semen obtained from some stallions results in lower seminal quality and viability when the seminal plasma (SP) is present. The objective of this study was to evaluate the effect of the removal of SP using a Sperm Filter on the viability of cooled stallion semen. For this purpose, 31 stallions were used. Their ejaculates were divided into three groups: CN, semen was diluted with an extender; FLT, SP was removed by filtration; and CT, SP was removed by centrifugation and cooled to 15°C for 24 hours. Sperm kinetics and plasma membrane integrity were evaluated immediately after collection (T0) and after 24 hours of refrigeration (T1). No difference (P > .05) was noted at T1 for total sperm motility (TM), progressive sperm motility, or plasma membrane integrity when semen samples from all the stallions were analyzed. However, when samples from stallions termed bad coolers were analyzed (TM = <30% at T1), a difference was observed in TM and progressive sperm motility for CN compared with FLT and CT at T1. Sperm recovery was greater when SP was removed using the filter (FLT) to that when the SP was removed by centrifugation (CN) (89% vs. 81%). Thus, we concluded that filtering with a Sperm Filter is an efficient and practical method for removal of SP from stallion ejaculates, with lower sperm loss than centrifugation. We also found that the presence of SP reduces the quality and viability of cooled semen from stallions whose semen is sensitive to the process of refrigeration. © 2013 Elsevier Inc.

New seminal plasma removal method for freezing stallion semen

Seminal plasma removal, an indispensable step in equine semen cryopreservation, is usually done by centrifugation, but this might cause mechanical damage to sperm. A new method for seminal plasma removal from stallion semen, namely a filter composed of a synthetic hydrophilic membrane (Sperm Filter, BotuPharma, Botucatu, Sao Paulo, Brazil), was recently proposed. The objective of this study was to test the use of the Sperm Filter in the removal of seminal plasma before freezing stallion semen. Ejaculates from 31 stallions were divided into two groups and cryopreserved. In group 1 (G1), seminal plasma was removed with the Sperm Filter, and in group 2 (G2), seminal plasma was removed by centrifugation (600× g for 10 minutes). There were no differences (P < 0.05) between G1 and G2 in sperm kinetic parameters or plasma membrane integrity before or after cryopreservation. However, sperm recovery rate was higher (P < 0.05) for G1 versus G2 (mean ± SD, 89.4 ± 7.4% vs. 80.9 ± 5.5%). Therefore, the Sperm Filter was as efficient as centrifugation in removing seminal plasma from the stallion ejaculate. However, filtering was more practical and had significantly fewer sperm lost than the centrifugation technique. © 2013 Elsevier Inc.

Morfologia, ultraestrutura e morfometria do tegumento da paca (Cuniculus paca Linnaeus, 1766) criada em cativeiro

Considering the lack of detailed information about the morphology of paca, which is the second largest rodent of Brazilian's fauna, with excellent meat quality and, due to the importance of the common integument, we described the morphology, ultrastructure and morphometry of the skin of eight pacas (Cuniculus paca), males and females, through comparative analysis of skin's segments of the cervical, dorsal and medial carpal portions. Macroscopic characteristics of the coat were studied. Part of the segments of cutaneous regions was analyzed by light microscopy, and the other part by scanning electron microscopy. We measured the thickness of dermis, epidermis, stratum corneum, the profiles of the collagen fibers of reticular dermis and the area of filled sebaceous gland cells. The results were analyzed by descriptive statistics and T test (p<0.001). The color of the coat is reddish brown with bristles arranged in groups. The architecture of the skin and skin appendages resembles those of mammals in general, although there are no sweat glands. According to the morphometric analysis, this study shows differences of the skin architecture between male and female pacas and also between various body regions in the same animal.

Alterações morfofisiológicas do tegumento de coelhos infestados por carrapatos Rhipicephalus sanguineus (Acari: Ixodidae) e expostos à selamectina (princípio ativo do acaricida Revolution®, Pfizer)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da separação materna e do alcoolismo sobre a vesícula seminal e a galândula de coagulação de ratos UCh (bebedores voluntários de etanol a 10%)

Pós-graduação em Biologia Geral e Aplicada - IBB

Morfologia, ultraestrutura e morfometria do tegumento da paca (Cuniculus paca)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação dos parâmetros espermáticos e caracterização do perfil bioquímico do plasma seminal de cães (Canis familiaris – LINNAEUS, 1758) com hiperplasia prostática benigna, tratados com toxina botulínica A

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação seminal e testicular de cães submetidos à administração de cisplatina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos morfométricos e histológicos do tegumento de equinos das raças Árabe e Quarto de Milha

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização eletroforética do perfil protéico e análise bioquímica do plasma seminal canino

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Viabilidade e capacitação espermáticas, geração de peróxido de hidrogênio(H2O2) e lipoperoxidação no sêmen ovino adicionado de plasma seminal ou antioxidante após a criopreservação

Pós-graduação em Medicina Veterinária - FMVZ

Anatomia e desenvolvimento pós-seminal de espécies de Bromeliaceae (Poales) da Amazônia - MT

Pós-graduação em Ciências Biológicas (Biologia Vegetal) - IBRC

Estudos bioquímicos de tegumento de soja brasileira: isolamento, purificação e caracterização de peroxidase com guaiacol como substrato

Pós-graduação em Biotecnologia - IQ

Comprometimento fisiológico e seminal de cães machos infectados experimentalmente por leptospira interrogans sorovar Canicola

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)