Avaliação da existência de isolamento reprodutivo entre distintos citótipos de veado-mateiro (Mazama americana) por meio de machos híbridos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Presença de isolamento pós-zigótico entre diferentes citótipos de Mazama americana: avaliação de fêmeas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização de duas linhagens simpátricas de Atta laevigata

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Contribuição à análise do processo de africanização de Apis mellifera (Hymenoptera, apidae): características do desenvolvimento das glândulas de Dufour e de veneno

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Estudos taxonômicos no gênero Blechnum L. (Pterophyta : blechnaceae) para as regiões sudeste e sul do Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de um anticorpo policlonal anti-Duffy de Bos taurus taurus para detecção e quantificação do antígeno em eritrócitos bovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Evolução cromossômica no veado-mateiro – Mazama americana (Mammalia; Cervidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Criotolerância de embriões Bos Taurus Indicus e Bos Taurus Taurus produzidos in vitro e in vivo

Pós-graduação em Medicina Veterinária - FMVZ

Salmonella spp. in meat-type quails (Coturnix coturnix coturnix) in the State of São Paulo, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bacteriophage- induced reduction in Salmonella Enteritidis counts in the crop of broiler chickens undergoing preslaughter feed withdrawal

Salmonella food poisoning is a public health problem. Feed withdrawal from broiler chickens before slaughter can favor the multiplication of Salmonella in the cecum and crop of contaminated animals and subsequently lead to contamination of carcasses in the processing plant. In the present study, a cocktail of lytic bacteriophages isolated from sewage water was orally administered to 45-d-old broiler chickens 1 h after they received an oral dose of 107 cfu/mL Salmonella enterica subspecies enterica serotype Enteritidis. Immediately after phage administration and 30 min, 1, 3, 6, and 12 h thereafter, groups of chicken were killed. Ceca and crops were analyzed for the presence of Salmonella. At 3 h posttreatment, there were 103 cfu/g and 101 cfu/g of cecal and crop suspension, respectively. At 6 h after treatment, the number of Salmonella was 103 cfu/g in the cecal suspension, but below the detection limit in the crops. our results suggest that bacteriophage therapy may be able to reduce the contamination of chicken carcasses by reducing the preslaughter load of Salmonella in the birds.

Assessing signatures of selection through variation in linkage disequilibrium between taurine and indicine cattle

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Genomic divergence of zebu and taurine cattle identified through high-density SNP genotyping

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Development of a Loop-Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples

Improved methods for the detection of Histoplasma capsulatum are needed in regions with limited resources in which the organism is endemic, where delayed diagnosis of progressive disseminated histoplasmosis (PDH) results in high mortality rates. We have investigated the use of a loop-mediated isothermal amplification (LAMP) assay to facilitate rapid inexpensive molecular diagnosis of this disease. Primers for LAMP were designed to amplify the Hcp100 locus of H. capsulatum. The sensitivity and limit of detection were evaluated using DNA extracted from 91 clinical isolates of known geographic subspecies, while the assay specificity was determined using DNA extracted from 50 other fungi and Mycobacterium tuberculosis. Urine specimens (n = 6) collected from HIV-positive individuals with culture- and antigen-proven histoplasmosis were evaluated using the LAMP assay. Specimens from healthy persons (n = 10) without evidence of histoplasmosis were used as assay controls. The Hcp100 LAMP assay was 100% sensitive and specific when tested with DNA extracted from culture isolates. The median limit of detection was <= 6 genomes (range, 1 to 300 genomes) for all except one geographic subspecies. The LAMP assay detected Hcp100 in 67% of antigen-positive urine specimens (4/6 specimens), and results were negative for Hcp100 in all healthy control urine specimens. We have shown that the Hcp100 LAMP assay is a rapid affordable assay that can be used to expedite culture confirmation of H. capsulatum in regions in which PDH is endemic. Further, our results indicate proof of the concept that the assay can be used to detect Histoplasma DNA in urine. Further evaluation of this assay using body fluid samples from a larger patient population is warranted.

Genome-wide association analysis of feed intake and residual feed intake in Nellore cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Recognition of two morpho-types in eastern South American brackens (Pteridium-Dennstaedtiaceae-Polypodiopsida)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)